ISfinder Mirror Site

ISMae27

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010296	ND	Microcystis aeruginosa	Microcystis aeruginosa NIES-843

DNA section

IS Length : 1140 bp

Ends

IR Length : 14/15

IRL : TACCATTTTTCAAAAAGTATGTCATACTTGGTAAAGGTATAGTTTGTGTG
IRR : TACCATTTTTCTAAATTCCTGACAGACATAGAGCTTCTAAAATCCAAGAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTCTACGGTA	TTA	CCCACGGTAT	3
ACCCGAAGAA	TTA	TAATTGGTGC	3
CGATCAAGTC	TAA	GCGATGATTG	3
CCAATGATTG	TAA	CACCAGATAT	3
TTCCCCAAAC	TTA	CATTAGTAAC	3
AGTAATAACC	TTA	GTCCTGTCCT	3
AAATTTGGTA	TTA	TGTGACAGTT	3
TACCTCCACC	TTA	ATTCCTTCTT	3
TTTTTCTACT	TGA	CTCAATTGGC	3

DNA sequence

TACCATTTTTCAAAAAGTATGTCATACTTGGTAAAGGTATAGTTTGTGTGGTAAAAAGGCAATGAGTGGAGTCCCTAATATTAATGTTGCTGAGTCAGTA
GAAGACTTAAAATCCTTGTTGAAGCAACAAGTAACCTCTTTAAACTTTGCTAAAGTACAATCCCTGTATCTACTAAAAATTAAGGAGGTAGAAACGGTTC
GTCATCTCGCCGTGTTAATAGGACGCTCAGAAAGAACTATTCATCGCTGGTTAAGTTGTTATCGAGAAGGAGGGATAGAAAATCTCTTGTCAGAACCAGA
AAAACTGGGAAGACCCAAAAAGATTTCAGTGGAAGAAGCCGCTCTAATTCAGAATGAATTAAAAGACCCAGAAGGATTTCAAAGTTATAAAGAAATTCAT
TTTTGGGTATCAATTATTTTAGAAATACCCACCAGTTATATAACTGTTTACCGTCTCGTAAGAAATGAATTACAAGCTAAATTAAAAGTGGCTCGACCTC
AAAATTTAAAACAATTACCAGGAGAAGTAAAAATATTCCAAAATAATCTATCTGAACAGCTACAAGCTTTACTAGAAAAGGAATCTGAAAAAGTTAGTCA
ATATTTAAAAGTCCGCTTCTGGTGTCAAGATGAAAGTCGCTTCGGCTGTCATACCATTGTCAGAGATAAAATAACAATTAAAGGCATAAAGCCCCTTGGT
AATTTTCAGTATAATTTTCAATATCTCTGGCTCTATGGTTTAATAGAACCTCGAACAGGTAGCAGTTTTTTCTATGAATTTTCTCATTTAGATGGGGAAT
GTTTTAATCAATATTTAACGCTTTTTTCTCAAGCTTTTTCTGAGGAATTACATATTATTCAATTAGATAATGCTCCCGCACATACGGCGACCGACCTAGA
AATACCTGATAATATTATCCTATTTTATCAACCTCCCTATTGTCCAGAAGTAAATCCAATTGAGAGAGTTTGGCTATATTTGAAAAACCTATTGGCCTGG
GGCAATTTTAACTCCCTTGATAACTTAAGAAGTAAACTTTACCATCTTTTAAATTCTCTATCCAATGACACGATCGGGTATTTGACGGGATGGTCTTGGA
TTTTAGAAGCTCTATGTCTGTCAGGAATTTAGAAAAATGGTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1071 bp	356 aa	62	1132	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSGVPNINVAESVEDLKSLLKQQVTSLNFAKVQSLYLLKIKEVETVRHLAVLIGRSERTIHRWLSCYREGGIENLLSEPEKLGRPKKISVEEAALIQNEL
KDPEGFQSYKEIHFWVSIILEIPTSYITVYRLVRNELQAKLKVARPQNLKQLPGEVKIFQNNLSEQLQALLEKESEKVSQYLKVRFWCQDESRFGCHTIV
RDKITIKGIKPLGNFQYNFQYLWLYGLIEPRTGSSFFYEFSHLDGECFNQYLTLFSQAFSEELHIIQLDNAPAHTATDLEIPDNIILFYQPPYCPEVNPI
ERVWLYLKNLLAWGNFNSLDNLRSKLYHLLNSLSNDTIGYLTGWSWILEALCLSGI

Blast result :

Comments

ISMae27 is 59% aa similar to ISAcma28.

References

1] Modified and submitted by ISfinder (2010)

2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.