ISTvo2

  • Family ISNCY
  • Group ISA1214
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_002689 ND Thermoplasma volcanium
Thermoplasma volcanium GSS1
DNA section
IS Length : 1201 bp

Ends


IR Length : 14/18

IRL : GGACAAAAATAGTCATTCATCTCCCTCATCATCTTTCTTGATGATCAGAC
IRR : GGGACATAATTAGTCTTATCCATTCATGAACATCAGGTTGTGCCAGAGTC

Insertion site


Left flankDirect repeatRight flankDR Length
AGAGTCTGATCGACTCTAAGAGTCGGGAATGTA0

DNA sequence

GGACAAAAATAGTCATTCATCTCCCTCATCATCTTTCTTGATGATCAGACACACCCTCTTTCCCAGATATTCCTTAGGACAATCAACTTTGGCACCGGAT
CCAAACTTTGTAACAGTACGTTCCATGTAAGCCTCTATGTTGTGCGCTGTGATCTCGTTTGCCTCCTTCACCTTAACCTTCTTTACAAATGTATATATAC
AGTATATATTATACTTTGTCATTCAACAATCCATGTATGAGGAATTCAGGGATAACATAAACTCCCTCATTGAGGAGTACAGGGAGAAGTTATCCATTCC
TCATGTCATGGACTGAAAGGAGTACGAGAAAAAATACAGATCCAGGCTGACCGCAGCCTCCAGGGAACTGCGATCACTGTTGCTGGAGGCATCATCCCGT
ATACGGGAGGATGAATTTGGTAGACCGTCAGTCATACATGAAGTTGACAGGGCACAGATCATACTGTCAAAAGAGATGTTCGACCTGAGCAACCGCAAGG
CTGCCTATCTCATGCCCCTCATGGGCATGGAATATGAGGTGTCCTATAAAACAGTCGAAAGGATCTATTCAGATGATGTTGTGCCAATGATAATGCACAA
CCACTTCGTACTCTCAGTGAAAAAGAGGGGCATTGATATATCGAACGCATGCAGGGACAGAACCGGTTATTCGCTGACAGTTTCAGACCACCACAGATCT
GTGAGGGAGAAGCTGGGAGAATCTGTGAAGCATGGAAAATACAGGTATTCATTTGCCATCATGGATCTGAAGACACGCATGTACATAGGATACGCATCCT
TTGTGAGATCGGAGAGTGATGCTTACCAGAAAGCTTCGAGGATTATCTCTGACCTCGGGATACAGATAAATTCAGTGAGGCTTAACAAGTAATATTCAGG
GCAGAGCATACTGGGTGATTTCTCAGATAACACAAGGATATTCATCATTCCAAAGAAGAATTCACGCATTCACGGTAAAAAAAGATGGAGGGACATAATT
GCCTCATTCATGAGCGATCCCATGGAGTTCCTCAAGGAATACTTCAAACGCAACAACTCTGAGGCAGGTTTCTCCTCAGACAAGCGAGCCACAGGACGGA
GGATATTCCAAAGGCGCAACGACAGGATTGAGACATCGATATTCATAAAGGGACTCTGGCACAACCTGATGTTCATGAATGGATAAGACTAATTATGTCC
C
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
210 bp69 aa13222-No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTKYNIYCIYTFVKKVKVKEANEITAHNIEAYMERTVTKFGSGAKVDCPKEYLGKRVCLIIKKDDEGDE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
950 bp349 aa1371086+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MLCAVISFASFTLTFFTNVYIQYILYFVIQQSMYEEFRDNINSLIEEYREKLSIPHVMD*KEYEKKYRSRLTAASRELRSLLLEASSRIREDEFGRPSVI
HEVDRAQIILSKEMFDLSNRKAAYLMPLMGMEYEVSYKTVERIYSDDVVPMIMHNHFVLSVKKRGIDISNACRDRTGYSLTVSDHHRSVREKLGESVKHG
KYRYSFAIMDLKTRMYIGYASFVRSESDAYQKASRIISDLGIQINSVRLNK*YSGQSILGDFSDNTRIFIIPKKNSRIHGKKRWRDIIASFMSDPMEFLK
EYFKRNNSEAGFSSDKRATGRRIFQRRNDRIETSIFIKGLWHNLMFMNG

 

Blast result :
Comments
ISTvo2 is 81% (ORF1) and 61% (ORF2) aa similar to ISA1214. We extended the transposase to the first methionine, so it is too long compared to the others members of this family.
The transposase is certainly inactive there are two STOP codon.
References
1] Kawashima,T., Amano,N., Koike,H., Makino,S., Higuchi,S., Kawashima-Ohya,Y., Watanabe,K., Yamazaki,M., Kanehori,K., Kawamoto,T., Nunoshiba,T., Yamamoto,Y., Aramaki,H., Makino,K. and Suzuki,M.
(2000) Proc. Natl. Acad. Sci. U.S.A. 97 (26), 14257-14262