ISfinder Mirror Site

ISSsu3

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Streptococcus suis	Streptococcus suis

DNA section

IS Length : 896 bp

Ends

IR Length : 20/25

IRL : TAGTTAAATGAAACAAAAACAGTACATTTATGATATAATGTATTTATGGC
IRR : TAGTCAACTGAAACAAGAGCAGGACGAAAGTGCCTCAAGAAAAGTATCGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTTTCAACTATGAAA	TA	ATTTACATACCGGTC	2

DNA sequence

TAGTTAAATGAAACAAAAACAGTACATTTATGATATAATGTATTTATGGCATATTCATTAGATTTTCGTAAAAAAGTTCTCGCATACTGTGAGAAAACCG
GCCGTATTACTGAAGCATCAGCTATTTTCCAAGTTTCACGTAACACTATCTATCAATGGCTAAAATTAAAAGAGAAAACCGGCGAGCTTCATCACCAAGT
TAAAGGAACCAAGCCAAGAAAAGTGGATAGAGATAAATTAAAGAATTATCTTGAAACTCATCCAGATGCTTATTTGACTGAAATAGCTTCTGAATTTGAC
TGTCATCCAACAGCTATTCATTACGCCCTCAAAGCTATGGGATATACTCGAAAAAAAAGAGCTGTACCTACTATGAACAAGACCCTGAAAAAGTAGAACT
GTTCCTTAAAGAATTGAATAACTTAAGCCACTTGACTCCTGTTTATATTGACGAGACAGGGTTTGAGACATGTTTTCATCGAGAATATGGTCGCTCTTTG
AAAGGTCAGTTGATAAAAGGTAAGGTCTCTGGAAGAAGATACCAGCGGATATCTTTAGTGGCAGGTCTCATAAATGGTGCGCTTATAGCCCCGATGACAT
ACAAAGATACTATGACGAGTGGCTTTTTCGAAGCTTGGTTCAAAATATTCTTACTACCCACTTTAGGTAAACCATCTGTTATCATCATGGACAATGCAAA
GTTTCATAGGATGAGTAAGCTAAAAGATTTATGCGAGGAGCAGGGACATAGACTTTTACCACTTCCTCCTTACTCACCGGAATATAATCCCATTGAGAAA
ATATGGGCTCACATCAAAAAACACCTCAGAAGAGTATTGCCAAATTGCGATACTTTTCTTGAGGCACTTTCGTCCTGCTCTTGTTTCAGTTGACTA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
351 bp	116 aa	46	396	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAYSLDFRKKVLAYCEKTGRITEASAIFQVSRNTIYQWLKLKEKTGELHHQVKGTKPRKVDRDKLKNYLETHPDAYLTEIASEFDCHPTAIHYALKAMGY
TRKKRAVPTMNKTLKK

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
549 bp	182 aa	345	893	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

YSKKKSCTYYEQDPEKVELFLKEMNNLSHLTPVYIDETGFETCFHREYGRSLKGQLIKGKVSGRRYQRISLVAGLINGALIAPMTYKDTMTSGFFEAWFK
IFLLPTLGKPSVIIMDNAKFHRMSKLKDLCEEQGHRLLPLPPYSPEYNPIEKIWAHIKKHLRRVLPNCDTFLEALSSCSCFS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
848 bp	282 aa	46	893	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAYSLDFRKKVLAYCEKTGRITEASAIFQVSRNTIYQWLKLKEKTGELHHQVKGTKPRKVDRDKLKNYLETHPDAYLTEIASEFDCHPTAIHYALKAMGY
TRKKKSCTYYEQDPEKVELFLKEMNNLSHLTPVYIDETGFETCFHREYGRSLKGQLIKGKVSGRRYQRISLVAGLINGALIAPMTYKDTMTSGFFEAWFK
IFLLPTLGKPSVIIMDNAKFHRMSKLKDLCEEQGHRLLPLPPYSPEYNPIEKIWAHIKKHLRRVLPNCDTFLEALSSCSCFS

Blast result :

Comments

Accession number : NZ_AAFA02000059 Genome in progress. ORF 3 is a fusion ORF.
ISSsu3 is 91% (ORF1) and 88% (ORF2) aa similar to IS630-Spn1.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Direct submission GenBank