ISfinder Mirror Site

ISBwe1

Family IS1595
Group ISPna2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010180	ND	Bacillus weihenstephanensis	Bacillus weihenstephanensis KBAB4 plasmid pBWB401

DNA section

IS Length : 1536 bp

Ends

IR Length : 22/26

IRL : GGCTTTGTTAAAGGTAAATGTTGATATTTGAAACCTATCCGAAATCTTCG
IRR : GGCTCTGTTAATGTTGAATGTTGATAAAAAGTATTAAATATGGGAAGACA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGGGACCTTGTTTTAATTTT		AAAAATTAAAGAAACAAGACTTT	0

DNA sequence

GGCTTTGTTAAAGGTAAATGTTGATATTTGAAACCTATCCGAAATCTTCGTATAATTGTATAAGAACAAACATTCGAGAGGTGTTAAGGTTGAGCAAAGC
GTTCAGTAATCTATTGAAACATATTGGCAATTTGTCACACACAGAGAAAGAGCAAGTGTTTCAATGGGTAAAACGCTATGTTGAACCTTCTTCCTCTGTC
GGTGGTCGTCTAATCAATGAAATGAGAGAAACTCGTTTCAAGGAAGGGTTTGAATGTCCTCATTGTGCATCCGAACACGTTGTTCGTTTCGGTAAACACA
ATAATCGCCAACGCTATCGTTGTAAATGTTGTAGTAAGACATTCACTGATACAACGAATACTGTTCTTTACCGTACTCGAAAAGGAAACGAATGGATTAC
ATTTGTTGATTGTATGTTTAAAGGATATTCCTTGCGAAAGTCCGCTGAAATCGTGGGGGTTACATGGGTTACACTTTTTTATTGGAGACATAAATTGTTA
AGTGCGTTAAAACAATTGGATTTTGAGAAATTTGAAGGTATCGTTGAAGTTGACGAGACCTATTTCCTATACTCTCAAAAGGGTCAACGTGGTATTTCAG
AACGCAAACCTCGTAGGCGTGGTGGAAAATCTAAACACAGAGGTATCAGCCACGAACAAGTTTGTGTTCTTGTTGCCAGAGACCGTACCAAAGCGACTGT
CTCCAAGATTGCTTGTATGGGGCGTGTTGTGAAAACAAAAGTTGATAGTATGATTGGTTCTAAATTAACATCGGATAATGTACTTGTTACAGATGCTTGG
AGAGCCTACAAAACGTATGCAAAAGAAAAAGGAATAGAGCATTACAGAATTAAATCAAACGATGGTAAGCACGTTATTAAAGGCTTGTACCATATCCAGA
ATGTAAATGGTCTCCATTCTCGTATGAAACAATGGATAGACCGTTTTAAAGGTGTGGCTACAAAATATTTAGACAACTACCTTGCGTGGTTCTTGTTTAT
AGATAGAAAAAGTAACGAAAGCACAAAACACAACATTAAAGAGTTCTTACTCACATCATTTGTATTTGAAATGACTGATACTTATGATAGTTTGCGTCTG
TCAAAATTCAGTGTATAAACGAACGAATTAAGAAAAGGGTGAAATATTGGATAACTCGAAATGGATATCTTTAAGTAATGTTAATGATGAGGAGGAAATT
TGGGCAGGGGCAAGAGTTAGGTTGTACAATGTTGGTATGAATAGAGAAGACAAAGAAAATGATTTCTACGAATACATTATTTCTTATATCTACGACAACG
ATAACTACTTACAACTAACTAATTTAACAACAGGAAAAGCAGGTTATATTATTTGTGTCATTGAAAAAGAATTGCCGAATAATTATGCTTTGGGTAAAAC
ATTAAAGCAAAGAATGGGCTTAGAAAATACCTATTTTAGATTTGAATATGAATAATCAACAACTAAGGGAAGCACCAACTCTAAGGTGTCTTCCCATATT
TAATACTTTTTATCAACATTCAACATTAACAGAGCC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1029 bp	342 aa	90	1118	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSKAFSNLLKHIGNLSHTEKEQVFQWVKRYVEPSSSVGGRLINEMRETRFKEGFECPHCASEHVVRFGKHNNRQRYRCKCCSKTFTDTTNTVLYRTRKGN
EWITFVDCMFKGYSLRKSAEIVGVTWVTLFYWRHKLLSALKQLDFEKFEGIVEVDETYFLYSQKGQRGISERKPRRRGGKSKHRGISHEQVCVLVARDRT
KATVSKIACMGRVVKTKVDSMIGSKLTSDNVLVTDAWRAYKTYAKEKGIEHYRIKSNDGKHVIKGLYHIQNVNGLHSRMKQWIDRFKGVATKYLDNYLAW
FLFIDRKSNESTKHNIKEFLLTSFVFEMTDTYDSLRLSKFSV

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
309 bp	102 aa	1147	1455	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDNSKWISLSNVNDEEEIWAGARVRLYNVGMNREDKENDFYEYIISYIYDNDNYLQLTNLTTGKAGYIICVIEKELPNNYALGKTLKQRMGLENTYFRFE
YE

Blast result :

Comments

ISBwe1 is 61% aa similar to ISBsp1.

References

1] Lapidus,A., Goltsman,E., Copeland,A., Lucas,S., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E.,Tice,H., Bruce,D., Pitluck,S., Richardson,P., Auger,S., Galleron,N., Sanchis,V., Gohar,M., Broussole,V., Brillard,J., Guinebretiere,M.-H., Ehrlich,S.D., Lereclus,D., Aymerich,S., Nguen-the,C. and Sorokin,A. (2006) Direct submission GenBank.