ISAzsp1
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ496234 | ND | Azotobacter sp. | Azotobacter sp. FA8 |
DNA section
IS Length : 1309 bp
Ends
IR Length : 25/28
IRL : TGAACCGCCCCGGGTTTCCCGGAGGCTCCAACCTTTGAGAAGATGGAGCC
IRR : TGAACCGCCCCGGGAATCTCGGAGGCTCTTTGGTGTGAGTCATGCCGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGTAGTCGATAAAGGG | CTTG | CGTGCAGTTGACGTGG | 4 |
DNA sequence
TGAACCGCCCCGGGTTTCCCGGAGGCTCCAACCTTTGAGAAGATGGAGCCATGAAGTCAGCAAGGAAGTATTCCCCTGAAGTCCGGCAACGGGCGGTTCG
CATGGTGCTCGAGCATCAGGACGAGCACGAGTCGCAGTGGGCGGCGATCAATTCCATCGCGGCGAAGATCGGCTGCACGGCCGAAACGCTGCGCCGCTGG
GTGAGGCAGGCCGAGCGCGATACCGGTCGGCGCGAGGGGCAGACGACGCCCGAGCGTGAACGGATCAAGGCGCTCGAGCGGGAAGTGCGTGAGCTGCGCC
AGGCCAACGAGATTCTGCGCAAGGCCTCGGCGTATTTTGCCCAGGCGGAGCTCGACCGCCGCTTCAAGCGATGAAGGCCTTCATCGACGATCACCGCCAG
GTCTACGGGGTCGAGCCGATCTGCAAGGTACTGCCGATTGCCCCGTCGACCTACTACGAGCATGTGACCCGGAAAATCGTCCCCGCACGGCAGTCAGCCC
GAGCACGCCGGGATGAACTGCTCTGCGAGCATGTTCAGCGTGTCTGGGACGAGAACTTCCAGGTCTATGGCGCGCGCAAGCTCTGGAGGCAGCTGTGCCG
GGAAGGCTTCGAGGTGGCGCGCTGCACGGTCGAGCGGCTGATGCGCAGACTGGGCCTGCAGGGCGTGGTGCGCGGCAAGACGCCTCGAACCACGGTCAGC
GACCGCGCGACACCGTGCCCGCGGGATCATGTCAATCGCCAGTTCCAAGCCAGCCGGGCGAATGCATTGTGGGTATCGGATTTCACTTATGTGTCGACCT
GGCAGGGTTTTGTCTACGTGGCCTTCGTCATCGATGTCTTCGCCCGACGGATCGTCGGCTGGAAGGTTTCCAGTTCGGCACGTACCGACTTCGTGCTGGA
TGCCCTGGAGCAGGCGCTGTATGCCCGTCAGCCGGTACGGGGCGGGCTGATCCATCATAGTGATAGAGGGGTGCAGTATGTCTCGATCCGCTACACGGAG
CGCCTGGCAGAGGCGGGTATCGAGCCGTCGGTGGGCAGTGTGGGGGACTCCTACGACAACGCCCTGGCCGAGACGATCAATGGCCTGTACAAGGCGGAGG
TGATTCATCGCCAGTCCTGGAAGAACCGCGAGGCCGTGGAGCTGGCCACGCTGACCTGGGTGGACTGGTACAACCACCGTCGACTGCTGGAGCCGATCGG
CAACATACCGCCGGCGGAAGCTGAAGCGACGTATTATCGACAACTCGACGAGTCAGCCCTGGCGGCATGACTCACACCAAAGAGCCTCCGAGATTCCCGG
GGCGGTTCA
CATGGTGCTCGAGCATCAGGACGAGCACGAGTCGCAGTGGGCGGCGATCAATTCCATCGCGGCGAAGATCGGCTGCACGGCCGAAACGCTGCGCCGCTGG
GTGAGGCAGGCCGAGCGCGATACCGGTCGGCGCGAGGGGCAGACGACGCCCGAGCGTGAACGGATCAAGGCGCTCGAGCGGGAAGTGCGTGAGCTGCGCC
AGGCCAACGAGATTCTGCGCAAGGCCTCGGCGTATTTTGCCCAGGCGGAGCTCGACCGCCGCTTCAAGCGATGAAGGCCTTCATCGACGATCACCGCCAG
GTCTACGGGGTCGAGCCGATCTGCAAGGTACTGCCGATTGCCCCGTCGACCTACTACGAGCATGTGACCCGGAAAATCGTCCCCGCACGGCAGTCAGCCC
GAGCACGCCGGGATGAACTGCTCTGCGAGCATGTTCAGCGTGTCTGGGACGAGAACTTCCAGGTCTATGGCGCGCGCAAGCTCTGGAGGCAGCTGTGCCG
GGAAGGCTTCGAGGTGGCGCGCTGCACGGTCGAGCGGCTGATGCGCAGACTGGGCCTGCAGGGCGTGGTGCGCGGCAAGACGCCTCGAACCACGGTCAGC
GACCGCGCGACACCGTGCCCGCGGGATCATGTCAATCGCCAGTTCCAAGCCAGCCGGGCGAATGCATTGTGGGTATCGGATTTCACTTATGTGTCGACCT
GGCAGGGTTTTGTCTACGTGGCCTTCGTCATCGATGTCTTCGCCCGACGGATCGTCGGCTGGAAGGTTTCCAGTTCGGCACGTACCGACTTCGTGCTGGA
TGCCCTGGAGCAGGCGCTGTATGCCCGTCAGCCGGTACGGGGCGGGCTGATCCATCATAGTGATAGAGGGGTGCAGTATGTCTCGATCCGCTACACGGAG
CGCCTGGCAGAGGCGGGTATCGAGCCGTCGGTGGGCAGTGTGGGGGACTCCTACGACAACGCCCTGGCCGAGACGATCAATGGCCTGTACAAGGCGGAGG
TGATTCATCGCCAGTCCTGGAAGAACCGCGAGGCCGTGGAGCTGGCCACGCTGACCTGGGTGGACTGGTACAACCACCGTCGACTGCTGGAGCCGATCGG
CAACATACCGCCGGCGGAAGCTGAAGCGACGTATTATCGACAACTCGACGAGTCAGCCCTGGCGGCATGACTCACACCAAAGAGCCTCCGAGATTCCCGG
GGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 324 bp | 107 aa | 51 | 374 | + | No |
Description : First part of the transposase
ORF sequence :
MKSARKYSPEVRQRAVRMVLEHQDEHESQWAAINSIAAKIGCTAETLRRWVRQAERDTGRREGQTTPERERIKALEREVRELRQANEILRKASAYFAQAE
LDRRFKR
LDRRFKR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 977 bp | 325 aa | 294 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
AAPGQRDSAQGLGVFCPGGARPPLQAMKAFIDDHRQVYGVEPICKVLPIAPSTYYEHVTRKIVPARQSARARRDELLCEHVQRVWDENFQVYGARKLWRQ
LCREGFEVARCTVERLMRRLGLQGVVRGKTPRTTVSDRATPCPRDHVNRQFQASRANALWVSDFTYVSTWQGFVYVAFVIDVFARRIVGWKVSSSARTDF
VLDALEQALYARQPVRGGLIHHSDRGVQYVSIRYTERLAEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRQSWKNREAVELATLTWVDWYNHRRLLE
PIGNIPPAEAEATYYRQLDESALAA
LCREGFEVARCTVERLMRRLGLQGVVRGKTPRTTVSDRATPCPRDHVNRQFQASRANALWVSDFTYVSTWQGFVYVAFVIDVFARRIVGWKVSSSARTDF
VLDALEQALYARQPVRGGLIHHSDRGVQYVSIRYTERLAEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRQSWKNREAVELATLTWVDWYNHRRLLE
PIGNIPPAEAEATYYRQLDESALAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 406 aa | 51 | 1270 | + | Yes |
Chemistry : DDE
ORF sequence :
MKSARKYSPEVRQRAVRMVLEHQDEHESQWAAINSIAAKIGCTAETLRRWVRQAERDTGRREGQTTPERERIKALEREVRELRQANEILRKASAYFCPGG
ARPPLQAMKAFIDDHRQVYGVEPICKVLPIAPSTYYEHVTRKIVPARQSARARRDELLCEHVQRVWDENFQVYGARKLWRQLCREGFEVARCTVERLMRR
LGLQGVVRGKTPRTTVSDRATPCPRDHVNRQFQASRANALWVSDFTYVSTWQGFVYVAFVIDVFARRIVGWKVSSSARTDFVLDALEQALYARQPVRGGL
IHHSDRGVQYVSIRYTERLAEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRQSWKNREAVELATLTWVDWYNHRRLLEPIGNIPPAEAEATYYRQLD
ESALAA
ARPPLQAMKAFIDDHRQVYGVEPICKVLPIAPSTYYEHVTRKIVPARQSARARRDELLCEHVQRVWDENFQVYGARKLWRQLCREGFEVARCTVERLMRR
LGLQGVVRGKTPRTTVSDRATPCPRDHVNRQFQASRANALWVSDFTYVSTWQGFVYVAFVIDVFARRIVGWKVSSSARTDFVLDALEQALYARQPVRGGL
IHHSDRGVQYVSIRYTERLAEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRQSWKNREAVELATLTWVDWYNHRRLLEPIGNIPPAEAEATYYRQLD
ESALAA
Blast result :
Comments
ISAzsp1 is 85% (ORFA) and 88% (ORFB) aa similar to IS51.
References
1] Julia Pettinari,M., Chaneton,L., Vazquez,G., Steinbuchel,A. and Mendez,B.S. (2003) Plasmid 50 (1), 36-44