ISBcen17
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Burkholderia cenocepacia | Burkholderia cenocepacia J2315 |
DNA section
IS Length : 1734 bp
Ends
IR Length : 27
IRL : GGCGCATTCAACCGGTCGTCGCAACACTCGATTGTTGATCAGATTTTAGA
IRR : GGCGCATTCAACCGGTCGTCGCAACACCTTTAATCGAAGAGGTGTTGCAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAAGGCTGG | CAA | TACCGGATGC | 3 |
DNA sequence
GGCGCATTCAACCGGTCGTCGCAACACTCGATTGTTGATCAGATTTTAGATATTCGTTCAGAGCTTCTGCCGGCGTCTTCCACCCGAGCGCCTTTCGAGG
CCTTGTGTTCAGTGTATGTGCAACGGCTTGGATCTCTCGTGCACTCCATCGGGATAGGTCTGTACCCTTCGGAAAGTATTGTCGCAATAGGCCGTTCGTA
TTCTCATTCGTGCCACGTTGCCATGGACTATGTGGGTCGGCGAAAAATACCTGTACACCGGACTCGATAGTGAAGCGAGCATGATCGGACTGAGCTTCCC
CTGAAATTTCGCCTTCCAATAGGTCGTGTGTAAACTCGACGGAAAGGAAGGAAATCAGGCATGTTCAAGGTACCGAAACAGTCGTACACGGCAGAGTTCA
AGTTGGCCGCCGTACGGCGGGTCAAGGATGGCCAGAGAGTGGCCGTGGTGGCCCGCGAGTTGGGCATTTCTGAGCAGACGCTTCGCAACTGGGTGAAGAG
CGAAGCGTCGGGCAAGCTCAACGGCGCCGGAGCGAAGCCGGTCACGCCGGAGCAGATGGAACTGTCGCGTCTGCGAGCGGAGAACAAGCGCCTGCAGATG
GAGCTTGAGATCGCAAAAAAAGCGGCAGCATACTTCGCGAAGGACCTGCTGTGAGGTATGCCTGGATCGACCGGCAGGTCGGGCAATATCCGCTGTCGTC
GTTGTGCTACGTGCTGTCGGTTAGCGTGAACGGTTATCGCGCGTGGAAGCGCGGCGGTAGACCGGGTCGAACGCGATTGACGGACACGCAATTGCTGACG
CTGATCCGGACGGTGCACGCCGAGGCCAAGGGTGCATACGGCTCGCCACGCATGACCAAGGAAATTCGTGAGCGCGGCTTTCCAGCCAGCAAGGAGCGCG
TGGAACGGCTGATGCGTGAGAACGGCATCCGGGCCCGGCACAAGCGCCGTTACCGAGCGACGACCGATTCGAAGCACAAGCTGCCGGTTGCGCCGAATCT
GTTGAATCGCGAGTTCACGCCCGCCGAGCCGAATCAGGTGTTCAGCTCGGACATCACGTACATCTGGACCGACGAGGGTTGGCTGTACTTGGCCGTGGTG
CTTGACCTGTTCAACCGGGAGGTGGTTGGCTGGTCGATCAGGCCGCGCATGACGGCGGATCTCGTGACGGACGCGCTGACGATGGCTTGGTTCCGCCGCC
GACCCGCGCCGGGCGCACTGTGCCACTCGGATCGGGGCAGCCAGTACGCGAGTCACGAGTTCCAGCGCAAACTGGCCAGCTACGGTATGCGCTGTTCGAT
GAGCCGCAAGGGAAATTGTTGGGATAACGCCCCGACGGAAAGCTTCTTCAACAGTTTGAAGAACGAGCGGGTCCACGCCACGCGCTATCGGACGCATCAG
GACGCGAAGGCCGATCTGTTCGAATACATCGAAGTGTTTTACAACCGCAGTCGTCGCCATTCGTCTCTCGGCCTCGTTTCTCCGGAGCAGTTCCTTCGGG
ACTGGATCAAAGCTCAGCAGACCAAGGATGCGGCTGCATAACTCGGGACCGTTGGAAGGCGAAAAACCGAGGGAACCTCAGTTCCCAAAACCTACGCTCG
ATCTCATTCCGAAGCGATGGGGCACCCGGCGAAGGCATCGCTTCTCGCCCCGTCAGTTGATATCCCCGTCCCGCCGTTCGCTTCATGCAACACCTCTTCG
ATTAAAGGTGTTGCGACGACCGGTTGAATGCGCC
CCTTGTGTTCAGTGTATGTGCAACGGCTTGGATCTCTCGTGCACTCCATCGGGATAGGTCTGTACCCTTCGGAAAGTATTGTCGCAATAGGCCGTTCGTA
TTCTCATTCGTGCCACGTTGCCATGGACTATGTGGGTCGGCGAAAAATACCTGTACACCGGACTCGATAGTGAAGCGAGCATGATCGGACTGAGCTTCCC
CTGAAATTTCGCCTTCCAATAGGTCGTGTGTAAACTCGACGGAAAGGAAGGAAATCAGGCATGTTCAAGGTACCGAAACAGTCGTACACGGCAGAGTTCA
AGTTGGCCGCCGTACGGCGGGTCAAGGATGGCCAGAGAGTGGCCGTGGTGGCCCGCGAGTTGGGCATTTCTGAGCAGACGCTTCGCAACTGGGTGAAGAG
CGAAGCGTCGGGCAAGCTCAACGGCGCCGGAGCGAAGCCGGTCACGCCGGAGCAGATGGAACTGTCGCGTCTGCGAGCGGAGAACAAGCGCCTGCAGATG
GAGCTTGAGATCGCAAAAAAAGCGGCAGCATACTTCGCGAAGGACCTGCTGTGAGGTATGCCTGGATCGACCGGCAGGTCGGGCAATATCCGCTGTCGTC
GTTGTGCTACGTGCTGTCGGTTAGCGTGAACGGTTATCGCGCGTGGAAGCGCGGCGGTAGACCGGGTCGAACGCGATTGACGGACACGCAATTGCTGACG
CTGATCCGGACGGTGCACGCCGAGGCCAAGGGTGCATACGGCTCGCCACGCATGACCAAGGAAATTCGTGAGCGCGGCTTTCCAGCCAGCAAGGAGCGCG
TGGAACGGCTGATGCGTGAGAACGGCATCCGGGCCCGGCACAAGCGCCGTTACCGAGCGACGACCGATTCGAAGCACAAGCTGCCGGTTGCGCCGAATCT
GTTGAATCGCGAGTTCACGCCCGCCGAGCCGAATCAGGTGTTCAGCTCGGACATCACGTACATCTGGACCGACGAGGGTTGGCTGTACTTGGCCGTGGTG
CTTGACCTGTTCAACCGGGAGGTGGTTGGCTGGTCGATCAGGCCGCGCATGACGGCGGATCTCGTGACGGACGCGCTGACGATGGCTTGGTTCCGCCGCC
GACCCGCGCCGGGCGCACTGTGCCACTCGGATCGGGGCAGCCAGTACGCGAGTCACGAGTTCCAGCGCAAACTGGCCAGCTACGGTATGCGCTGTTCGAT
GAGCCGCAAGGGAAATTGTTGGGATAACGCCCCGACGGAAAGCTTCTTCAACAGTTTGAAGAACGAGCGGGTCCACGCCACGCGCTATCGGACGCATCAG
GACGCGAAGGCCGATCTGTTCGAATACATCGAAGTGTTTTACAACCGCAGTCGTCGCCATTCGTCTCTCGGCCTCGTTTCTCCGGAGCAGTTCCTTCGGG
ACTGGATCAAAGCTCAGCAGACCAAGGATGCGGCTGCATAACTCGGGACCGTTGGAAGGCGAAAAACCGAGGGAACCTCAGTTCCCAAAACCTACGCTCG
ATCTCATTCCGAAGCGATGGGGCACCCGGCGAAGGCATCGCTTCTCGCCCCGTCAGTTGATATCCCCGTCCCGCCGTTCGCTTCATGCAACACCTCTTCG
ATTAAAGGTGTTGCGACGACCGGTTGAATGCGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 97 aa | 361 | 651 | + | No |
Description : First part of the transposase
ORF sequence :
MFKVPKQSYTAEFKLAAVRRVKDGQRVAVVARELGISEQTLRNWVKSEASGKLNGAGAKPVTPEQMELSRLRAENKRLQMELEIAKKAAAYFAKDLL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 310 aa | 609 | 1538 | + | No |
Description : Second part of the transposase
ORF sequence :
DRKKSGSILREGPAVRYAWIDRQVGQYPLSSLCYVLSVSVNGYRAWKRGGRPGRTRLTDTQLLTLIRTVHAEAKGAYGSPRMTKEIRERGFPASKERVER
LMRENGIRARHKRRYRATTDSKHKLPVAPNLLNREFTPAEPNQVFSSDITYIWTDEGWLYLAVVLDLFNREVVGWSIRPRMTADLVTDALTMAWFRRRPA
PGALCHSDRGSQYASHEFQRKLASYGMRCSMSRKGNCWDNAPTESFFNSLKNERVHATRYRTHQDAKADLFEYIEVFYNRSRRHSSLGLVSPEQFLRDWI
KAQQTKDAAA
LMRENGIRARHKRRYRATTDSKHKLPVAPNLLNREFTPAEPNQVFSSDITYIWTDEGWLYLAVVLDLFNREVVGWSIRPRMTADLVTDALTMAWFRRRPA
PGALCHSDRGSQYASHEFQRKLASYGMRCSMSRKGNCWDNAPTESFFNSLKNERVHATRYRTHQDAKADLFEYIEVFYNRSRRHSSLGLVSPEQFLRDWI
KAQQTKDAAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1178 bp | 393 aa | 361 | 1538 | + | Yes |
Chemistry : DDE
ORF sequence :
MFKVPKQSYTAEFKLAAVRRVKDGQRVAVVARELGISEQTLRNWVKSEASGKLNGAGAKPVTPEQMELSRLRAENKRLQMELEIAKKSGSILREGPAVRY
AWIDRQVGQYPLSSLCYVLSVSVNGYRAWKRGGRPGRTRLTDTQLLTLIRTVHAEAKGAYGSPRMTKEIRERGFPASKERVERLMRENGIRARHKRRYRA
TTDSKHKLPVAPNLLNREFTPAEPNQVFSSDITYIWTDEGWLYLAVVLDLFNREVVGWSIRPRMTADLVTDALTMAWFRRRPAPGALCHSDRGSQYASHE
FQRKLASYGMRCSMSRKGNCWDNAPTESFFNSLKNERVHATRYRTHQDAKADLFEYIEVFYNRSRRHSSLGLVSPEQFLRDWIKAQQTKDAAA
AWIDRQVGQYPLSSLCYVLSVSVNGYRAWKRGGRPGRTRLTDTQLLTLIRTVHAEAKGAYGSPRMTKEIRERGFPASKERVERLMRENGIRARHKRRYRA
TTDSKHKLPVAPNLLNREFTPAEPNQVFSSDITYIWTDEGWLYLAVVLDLFNREVVGWSIRPRMTADLVTDALTMAWFRRRPAPGALCHSDRGSQYASHE
FQRKLASYGMRCSMSRKGNCWDNAPTESFFNSLKNERVHATRYRTHQDAKADLFEYIEVFYNRSRRHSSLGLVSPEQFLRDWIKAQQTKDAAA
Blast result :
Comments
1 copy within J2315. Ends defined by inverted repeats, and insertional context (pseudogene). ISBcen17 is interrupted by ISBcen18.
ISBcen17 is 91% (ORF1) and 93% (ORF2) aa similar to ISRso8.
Note : the ends of this IS are different of those of the others IS3 family members.
ISBcen17 is 91% (ORF1) and 93% (ORF2) aa similar to ISRso8.
Note : the ends of this IS are different of those of the others IS3 family members.
References
1] The Wellcome Trust Sanger Institute. http://www.sanger.ac.uk
2] Seth-Smith, H. (2006) Direct submission.
2] Seth-Smith, H. (2006) Direct submission.