ISAba125

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AY751533 ND Acinetobacter baumannii
Acinetobacter baumannii
DNA section
IS Length : 1087 bp

Ends


IR Length : 20/26

IRL : GGCAGAGTAAAACTTGAAGTGCGACATAAACCACCTAATTAATTTAAAGG
IRR : GGCGAATTCAAACATGAGGTGCGACAGTTTCAAAAGCCATATGATAATCA

Insertion site


Left flankDirect repeatRight flankDR Length
TACTTAGATAATGATTATGACCT3

DNA sequence

GGCAGAGTAAAACTTGAAGTGCGACATAAACCACCTAATTAATTTAAAGGGTTTATGGAGTATATAAAATTGTCATACCATCATCTTAACTTTGAAGATC
GTACTGCATTAATGCTTGAGTCAAGAAAAGAAGGCTTTTCAGCCAGAAAATTTGCTGAACTTATTAAAAGACATCCTAGTACGATCTATCGTGAGCTTAA
AAGAAATAGCATCAATGACGTTTATCAAGCTCAATATGCTTCTGATAACACTTTTGCTAGACGTAGACGTGGTCACAGAAAACTCAAAATCGATTCAATC
CTCTGGAAATTTATTGTTGAAGCGATCCGTTGTTTATGGTCTCCTCAGCAAATAGCAAAGCGTTTAAAGACATTTCCTGATTTGGATCAAACAATGAATG
TAAGCCATACAACGATTTATTCAACGATACGAGCATTACCCAAGGGTGAGTTGAAAAAAGACTTATTATCCTGTCTACGTCATGAAAATAAAAAGCGAAA
AGCTAACGGTGAACCTAAAAAAGATTCTATATTACAGGATATTAAAACTATTCATGAGCGCCCAGCCGAAGTTCAAGAAAGAAAAATACCGGGTCATTGG
GAAGCTGATTTAATTAAAGGTAAAGACAATAAAAGTTCGATAGCAACACTTATTGAACGAAATACACGGCTCTGTATCTTGGCAACATTACCTGATGCAA
AGGCAGAATCAGTGCGCAAGGCTTTAACTGAAGCTCTGAAATATTTACCTGCAGAACTGCGTAAAACGTTGACCCATGACCGTGGACGTGAGATGTCAGA
ACATAAAATACTCGAAGAAGATTTAGGCATAGATGTATATTTCTGTGACCCACATTCACCCTGGCAAAAAGGCACATGCGAAAATATGAATGGTTTAATT
AGGCAATATTTACCTAAAGGGATTGATTTAAATCAGGCAGATCAGCATTATTTAAATCAAGTTGCCATGTCACTGAATACTCGTCCTAGAAAGGCGTTAG
ATTGGCTTACACCATTAGAGAAATTTGCTCAGCTTGTTGATTATCATATGGCTTTTGAAACTGTCGCACCTCATGTTTGAATTCGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
969 bp322 aa1121080+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLESRKEGFSARKFAELIKRHPSTIYRELKRNSINDVYQAQYASDNTFARRRRGHRKLKIDSILWKFIVEAIRCLWSPQQIAKRLKTFPDLDQTMNVSHT
TIYSTIRALPKGELKKDLLSCLRHENKKRKANGEPKKDSILQDIKTIHERPAEVQERKIPGHWEADLIKGKDNKSSIATLIERNTRLCILATLPDAKAES
VRKALTEALKYLPAELRKTLTHDRGREMSEHKILEEDLGIDVYFCDPHSPWQKGTCENMNGLIRQYLPKGIDLNQADQHYLNQVAMSLNTRPRKALDWLT
PLEKFAQLVDYHMAFETVAPHV

 

Blast result :
Comments
ISAba125 is 66% aa similar to ISC1041.The "carO" gene is disrupted by ISAba125 insertion.
The file was corrected by Sally Partridge indication the 14/09/2011.
References
1] Mussi,M.A., Limansky,A.S. and Viale,A.M.(2005) Antimicrob. Agents Chemother. 49 (4), 1432-1440