ISAba5

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Acinetobacter baumannii
Acinetobacter baumannii
DNA section
IS Length : 1044 bp

Ends


IR Length : 15/16

IRL : GGCTTTGTTGCACAAACCTATCTGTAACGGCTTTTTCAGCGATATAATTT
IRR : GGCTTTGTTGCATAAAGGTTTGAAAGATAGAAGCGATCCTAATTGAGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
TTCAACTAAGCTATAAAAAT0

DNA sequence

GGCTTTGTTGCACAAACCTATCTGTAACGGCTTTTTCAGCGATATAATTTTTAAAAATGAAGAAGCCTACACACAAAATCTACCGCACAACCAATTGGCC
CGCATATAACCGAGCACTCATGAGTCGCGGAAATATTGCCATTTGGTTTGATCCTGCTACGCAATGGTATGCTCCATCAAAAGGCAAACAAGGGCGAAAT
CAAACCTACTCCGACGCAGCTATCCAATGCTGGTTAATGATTAAATCCTTATTCCGTCTATCTTTACATATGGTCACTGGCTTTGTCCAAAGTCTGATTA
AAATTTGTGTATTAAATTGGACAGCACCAGATTACACCACGCTTTGTAGAAGACAAAAGCATATTGATATTGCAATTAGCTACCAAAAAAGTAGAGATGG
TCTGCATCTACTCGTGGACTCTACTGGCTTGAAATTTCTAGGTGGAGGTGAATGGAAATGCAAGAAACATGGATCTGAATATCGTCGCCAATGGCGTAAG
CTCCATATTGCTATAGAAGCTGAAACCCTTCAAATACGTACAGTACAACTCACGACAAATAATGTCAGCGATTCACAAGTGCTCGAAGATTTACTTTCTC
AAGTTCCCTTGGATGAACGAATTGATTCAGTTTATACAGATGGTGCTTATGACACAAAGCACTGCCGACAAGTCATTTTAAATCGTGATGCACATGCGGT
CATTCCACCCAGGAAGAATACAAAGCCTCGGAAAGATTAGAACAAGTCAGGTCTATAGAGCGAAATGAGTTATTGAAAACAGTCAAACTGCTAGGAAGGT
CACTTTGGAAAAAGTGGTCTGGTTATCACCGTCGAAGTTTGGTGGAAACCAAGATGCATTGCATCAAATTATTAGGCGATAAATTAACAGCAAGGAGTTT
TCCTAGTCAGGTGAATGAGATTCATGCACGCGTAGCCCTTTTGAACAAATTCACAGAATTGGGGCGCCCTCACACCCAAGTTGTCACTTGAATTTGGCTC
AATTAGGATCGCTTCTATCTTTCAAACCTTTATGCAACAAAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
872 bp291 aa120991+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRGNIAIWFDPATQWYAPSKGKQGRNQTYSDAAIQCWLMIKSLFRLSLHMVTGFVQSLIKICVLNWTAPDYTTLCRRQKHIDIAISYQKSRDGLHLLVD
STGLKFLGGGEWKCKKHGSEYRRQWRKLHIAIEAETLQIRTVQLTTNNVSDSQVLEDLLSQVPLDERIDSVYTDGAYDTKHCRQVILNRDAHAVIPPRKN
TKPRKD*NKSGLSIERNELLKTVKLLGRSLWKKWSGYHRRSLVETKMHCIKLLGDKLTARSFPSQVNEIHARVALLNKFTELGRPHTQVVT

 

Blast result :
Comments
Identified on a plasmid.
ISAba5 is 91% aa similar to IS17.
The transposase of ISAba5 is mutated, there is a STOP codon in frame and one frameshift, the transposase is probably inactive.
References
1] Poirel, L. (2006) Direct submission.