ISfinder Mirror Site

ISTac1

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_002578	ND	Thermoplasma acidophilum	Thermoplasma acidophilum

DNA section

IS Length : 1569 bp

Ends

Left end : GTAAGAAATAGCAAGAAAGCTCCGATCTTCAGAGAGGAGATGAATTGCGGAAGAGTATATGTATGATAATAGTATATGATATTCGATATATGGAAATAAG II struct. : Yes
Right end : GTGATGCATGCAAGGGGAGCACTGATACCCCTGAAGAGGCAACCCCGGGAACGACGGGGACCTTAGAACCTCCGAAGCTTTAGCGAGGAGAGTATGTCAG II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TGTAAGCTGACCCATAAAA	TTAC	CATGAAGGTATAGTTTATCAGAAC	TCAG

DNA sequence

GTAAGAAATAGCAAGAAAGCTCCGATCTTCAGAGAGGAGATGAATTGCGGAAGAGTATATGTATGATAATAGTATATGATATTCGATATATGGAAATAAG
TAATGTAGAAAAGGAATACCACCACGAACCACATCTGGTGTATAGTTGCCAATACCATGTCATATTCTGCCCAAAATACAGGCGAAAAGTCCTAGTGGAC
GGTATTGATAAAAGGCTGAAAGAGTTGATCCTTGAGAAACAGAACGAATATCAGTACAAAATCCTTGAAATGGAGGTGATGCCTGACCATGTGCATCTCC
TCATAGACGTTGATCCGAGAATGGGTGTATACCATGTCGTGAACAGGATCAAGGGGTACACGTCACGTGTTCTGAGGGCGGAATTCCCGGAGCTGAGGAA
AAGATTACCCACATTGTGGACGCATTCAAAATTCATATCCTCTGTTGGCGCAGTAACGCTTGAAGTTGTGCAAAAATACATAGAGGATCAGAAGGGAAAA
TGATATTAACATTCAAAATCAAGCATGGAAGGGATTTTTCTGCGGAACTGAAGAAGGCAAAACAGATAGCTGAATTTGCAGTAAAAACGCACACGCTATC
ATCAAAAGATGTTAAGCAGTTCGGACTGAAGAGTATTATAGCGAACCAGATACTGAGAAAATATTCGAGGAATAAACAAATAAAGAAGGTAAAGAGTGTT
AAGCTTACGGTACCGAATCAGGGCATTAAAGTTGATAAAGAAAAAAGGGAAATATATATCCCGTCGCTGAAACTGACCCTCACATACATGTTCAGAAACG
ATTTCGAGAAGATAAACCAGATCGAGATCGATGAATGCTACGCATACGTATCGGTAAATATACCCGAAAAGAAGATGATTGAACCTAAAAAGTGGATAGG
CATAGACAGGAACGCCACAGGCCATATTGCTGTTGTTGCAGATCCTCAGACAGGTAAAATATGGAAATTGGGGAAAAAAGCAGAGCACATACACAATAAA
TACAAAGCAATCAGAAAGAAATTGCAAAAGAAGAAGAAATACAAGTTACTGAAAAAAATTAAAGACAGAGAGAGCAGAATAGTCAGAGACTTGAACCACA
AAGTTTCGAAGAAGATAGTTGAGATAGCACAAGGGGAGAATGCAGGAATAAAACTTGAGAAACTCAACGGAATAAGAAGCAACAAAAAACACACAAAGCC
GTTCAAATATGGCCTGAATTCATGGTCGTTCTACCAGCTCGAATTCTTCATACAGTATAAAGCTAAAATGAACGGCATCCCCCTAACCTATGTTGATCCT
AGATACACATCGAAAGAGTGCTCTAGGTGTGGATACATAGGTATTAGGGAGGACAAATCATTTGAGTGCCCGAGGTGCGGGCACGTTGACCATGCCGACG
CCAACGCCTCGTTCAATATAGCGGTGCGTCCTCCTTTGATGGAAGGCACAGGTCGATTGCATGCAGACAGTGATGCATGCAAGGGGAGCACTGATACCCC
TGAAGAGGCAACCCCGGGAACGACGGGGACCTTAGAACCTCCGAAGCTTTAGCGAGGAGAGTATGTCAG

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
414 bp	137 aa	90	503	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MEISNVEKEYHHEPHLVYSCQYHVIFCPKYRRKVLVDGIDKRLKELILEKQNEYQYKILEMEVMPDHVHLLIDVDPRMGVYHVVNRIKGYTSRVLRAEFP
ELRKRLPTLWTHSKFISSVGAVTLEVVQKYIEDQKGK

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1053 bp	350 aa	500	1552	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MILTFKIKHGRDFSAELKKAKQIAEFAVKTHTLSSKDVKQFGLKSIIANQILRKYSRNKQIKKVKSVKLTVPNQGIKVDKEKREIYIPSLKLTLTYMFRN
DFEKINQIEIDECYAYVSVNIPEKKMIEPKKWIGIDRNATGHIAVVADPQTGKIWKLGKKAEHIHNKYKAIRKKLQKKKKYKLLKKIKDRESRIVRDLNH
KVSKKIVEIAQGENAGIKLEKLNGIRSNKKHTKPFKYGLNSWSFYQLEFFIQYKAKMNGIPLTYVDPRYTSKECSRCGYIGIREDKSFECPRCGHVDHAD
ANASFNIAVRPPLMEGTGRLHADSDACKGSTDTPEEATPGTTGTLEPPKL

Blast result :

Comments

ISTac1 is 79% (ORF A) and 49% (ORF B) aa similar to ISClte2. There is only one copy of ISTac1 in Thermoplasma acidophilum genome.

References

1]Ruepp,A., Graml,W., Santos-Martinez,M.L., Koretke,K.K., Volker,C., Mewes,H.W., Frishman,D., Stocker,S., Lupas,A.N. and Baumeister,W.(2000) Nature 407 (6803), 508-513