ISfinder Mirror Site

ISNsp3

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_003276	ND	Nostoc sp.	Nostoc sp. PCC 7120 Nostoc sp. PCC 7120 plasmid pCC7120alpha

DNA section

IS Length : 1680 bp

Ends

Left end : GAGTCGTGATGCGTAAAGCCCCCGTCATTCATGCGGGGGATATAAGCGCTGGGGGATGAATTTATTCATCCGTGATGTGGGTCATTTATGTACTCCAAAA II struct. : Yes
Right end : GGGGCATCGACTGTTGGGTTAGGTGATGTCAGACTGGCTACGCCAGCAATCGCTGTTTGAGCCTAGAATCCCCCGAATTATATTCGGGGGAGTATGTCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TGGTGTGAGAACCAGAAGCATTGAGT	TTAC	AAGCCTCAATTCCTGAATTAGTTGAGGTGA	TCAA

DNA sequence

GAGTCGTGATGCGTAAAGCCCCCGTCATTCATGCGGGGGATATAAGCGCTGGGGGATGAATTTATTCATCCGTGATGTGGGTCATTTATGTACTCCAAAA
CTTTCTGAGTGCTGACTTTGCCCGTTGTGTCAAACATGAAAGAGTGAGTCCACAAACTAGGAAGCTTCATTAGTTCTGGAAATTCTTGCCTCAAGTATCT
TGATGACCTGCCTTTAAACGCTTTCACTACTTGAGATATTGAGTGATGCGGGTCATATTCTACCAACAAATGAATGTGGTCAGGAGCAATTTCTAGTGCT
TTGATAATCCACTTTTTATCATTAGCAACTGACTGAAATATCTCAATACATCGTAGTTTTAATTTTTCGTTGTTATTGAAAACTCTCCTGCGCCTACAGG
GTATCCAGACTAAGTGAACAGTAGCTAAACCTATTGAATGGTTGTAATGATTATATGTAGTTTTCATTTGTAGATGAGTGTTGATTATCTACACCAATGA
TGATATTATAACCACATGAAAACCTTGAAGTTCAAACTGTACCAGCACAAAAGAAATAGATTCCTCAAGCGCATGATTAATGCGAGTGGGGTAATCTACA
ACCATTGTATTGCCCTACACAAACGCTACTACCGAATGTGGGGCAAACACTTAAGTTGTGCAAAACTTCAAGCTCACATTGCTAAACTGCGGAAGCGTAA
CCCATTTTGGCAAACTGTAGGATCTCAGGCAGTGCAAGATATCTGTCAACGCATTGAAAAAGCTTACCAATTATTTTTTAAACACAATAAGAAGGGGGTT
AGACCACCAGGATTTAAGAAGGTGAAGAAATATAAATCCTTCACCCTAAAACAAGCAGGTTATAAGTTTTTAGGTGGCAACAGAGTAAAAATCGGTAATC
GAGTTTATCAATTCTGGAAGTCGAGACAGATAGAGGGAACAGTTAAAACCCTAACTATAAAGCGCACACCGTTAGGTGAGTTGTTCGTGGTTGTGGTAGT
TGACGACGGTATTAAGCCAGAAATCGAGATTAAGACGGGTAAAATCGCTGGTTTTGACTTTGGTTTGAAGACATTTCTCACTTGCTCAGACGGCACAAAA
ATTGATTCTCCTCAGTTTTTCAAACAATCTCTAAACGCTATCACCAAAGCTAGTAGACAACACTCAAAGAAACTCAAAGGTTCGGCTAATCGGAAACGAG
CGAGACTTAATCTAGTACGCAAGTACGAAGATATTTCTAATCGCCGTCGTGACTGGTTCTGGAAACTAGCTTATGAGTTAACAGATAAGTTTGATGTTCT
CTGCTTTGAAACCTTAAACCTCAAGGGAATGCAACGACGTTGGGGCAGGAAAATATCAGATTTGGCGTTTGGTGAGTTTCTGGAAATTCTAGAATGGGTT
GCCAAAAAGAAGAATAAACTGATTGTCTTCATTGATCAGTGGTATCCCAGCACTAAGACCTGTTCTCACTGTGGTCATGTCTTAGAGAGTATTGATTTGT
CTGTTCGGTCGTGGAGTTGTCCGTCCTGCCAGTCAGTGAACGAGAGAGACGAAAACGCCTCTAAGGTAATTTGTGCAGTCGGGGCATCGACTGTTGGGTT
AGGTGATGTCAGACTGGCTACGCCAGCAATCGCTGTTTGAGCCTAGAATCCCCCGAATTATATTCGGGGGAGTATGTCAA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
402 bp	133 aa	467	66	-	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKTTYNHYNHSIGLATVHLVWIPCRRRRVFNNNEKLKLRCIEIFQSVANDKKWIIKALEIAPDHIHLLVEYDPHHSISQVVKAFKGRSSRYLRQEFPELM
KLPSLWTHSFMFDTTGKVSTQKVLEYINDPHHG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1125 bp	374 aa	516	1640	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKTLKFKLYQHKRNRFLKRMINASGVIYNHCIALHKRYYRMWGKHLSCAKLQAHIAKLRKRNPFWQTVGSQAVQDICQRIEKAYQLFFKHNKKGVRPPGF
KKVKKYKSFTLKQAGYKFLGGNRVKIGNRVYQFWKSRQIEGTVKTLTIKRTPLGELFVVVVVDDGIKPEIEIKTGKIAGFDFGLKTFLTCSDGTKIDSPQ
FFKQSLNAITKASRQHSKKLKGSANRKRARLNLVRKYEDISNRRRDWFWKLAYELTDKFDVLCFETLNLKGMQRRWGRKISDLAFGEFLEILEWVAKKKN
KLIVFIDQWYPSTKTCSHCGHVLESIDLSVRSWSCPSCQSVNERDENASKVICAVGASTVGLGDVRLATPAIAV

Blast result :

Comments

ORF A coordinates are complement (66-467). ISNsp3 is 56% aa (ORF A) and 60% aa (ORF B) similar to IS608.

References

1] Kaneko,T., Nakamura,Y., Wolk,C.P., Kuritz,T., Sasamoto,S., Watanabe,A., Iriguchi,M., Ishikawa,A., Kawashima,K., Kimura,T., Kishida,Y., Kohara,M., Matsumoto,M., Matsuno,A., Muraki,A., Nakazaki,N., Shimpo,S., Sugimoto,M., Takazawa,M., Yamada,M., Yasuda,M. and Tabata,S. (2001) DNA Res. 8 (5), 205-213