ISAs4

  • Family IS5
  • Group IS903
MGE type ISRelated element(s) :
Isoform Synonym(s) ISAsa4
Accession numberTranspositionOriginHost
AY613940 ND Aeromonas salmonicida
Aeromonas salmonicida subsp. masoucida
Aeromonas salmonicida subsp. achromogenes
DNA section
IS Length : 1062 bp

Ends


IR Length : 16/18

IRL : GGCGTTGTTTCCTAAATCGATGCAGCTTGAATGGAACTAATTGAAAACCC
IRR : GGCGTTGTTGCCCAAATCAGCCAGCAAATCATGATTTCCCCAGCCCCAAG

Insertion site


Left flankDirect repeatRight flankDR Length
ACTTCCTGCATATCAGACCTATACGCAGA9

DNA sequence

GGCGTTGTTTCCTAAATCGATGCAGCTTGAATGGAACTAATTGAAAACCCAGTGATCTGAGCACCACCATCCCCCGCAAGTCTGCATCATGGGTAAAGCG
CTTCACCCCATCACCAATTGGCCTCAATACAACAGGTCTTTGATCAACCGTGGCTCTCTCACTTTCTGGGTTGATGCCGAGGCCATGGATAACTGGTTTC
ACCAAGACCATCACGGAAAACGGGGACGCAGTCAGCTCTACACCGACCAGAGCATCTGTACCTTTCTGATGCTCAAAGGGATTTTCAGCCTCACGCTGCG
AGCCACTCAGGGGCTACTCGACTCCCTGTTCGAGCTGATGAATGTGCCGCTGTGTGCCCCGGACTACAGCTGTGTCAGCAAGCGTGCACGCACAGTGAAG
GTGGCCTATCGACAGCCCCCTAAGGGGCGCATTACCGACCTGGTTATCGACTCGACGGGTCTGAAAGTGTTTGGTGAAGGCGAGTGGAAAGTCAGAAAAC
ACGGCGCTGAGAAACGTCGAGTCTGGCGCAAGTTGCATCTGGCGGTAGATCCGGTGACTCACGATATCGTGGCGGCAGAAGTGTCGCTGGAGAATGTGCA
TGACGCTGAGGTGTTGCCGACCTTGCTCAATCCTCTGCGTCGCAAGCTGGGGCGTGTTTACGCGGATGGCGCCTATGACAGCAAAGCCAGCCATCGGTTG
ATTGCGCGTAAAGGGGCGACGGCGTGTATCCCGCCTCGCAAGAACGCAGGGCTATGGAAAAAGGGCCATCCGAGAAATGAGGCGGTGCAGGTGATGCGCA
AGGAAGGGCTGGCGCACTGGAAGAAGATATCGGGGTATCACCGTCGTTCGCTGGCAGAGACAGCGATGTTCCGGTTCAAACAGTTAATGGCAGGCCAAAT
CACCCTGCGAAAATACAATGGTCAAGTGGGAGAAGTGATGGCGTATGTGAGCGCGATAAACAAACTGAACACCCTTGGTCTGCCTGTCAGAAAGCCCCGA
GTGTAACGGTCACTTGGGGCTGGGGAAATCATGATTTGCTGGCTGATTTGGGCAACAACGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
919 bp306 aa881006+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGKALHPITNWPQYNRSLINRGSLTFWVDAEAMDNWFHQDHHGKRGRSQLYTDQSICTFLMLKGIFSLTLRATQGLLDSLFELMNVPLCAPDYSCVSKRA
RTVKVAYRQPPKGRITDLVIDSTGLKVFGEGEWKVRKHGAEKRRVWRKLHLAVDPVTHDIVAAEVSLENVHDAEVLPTLLNPLRRKLGRVYADGAYDSKA
SHRLIARKGATACIPPRKNAGLWKKGHPRNEAVQVMRKEGLAHWKKISGYHRRSLAETAMFRFKQLMAGQITLRKYNGQVGEVMAYVSAINKLNTLGLPV
RKPRV

 

Blast result :
Comments
ISAs4 is 76% aa similar to ISVa1.
ISAs4 is also found in Aeromonas salmonicida strains designated as atypical.
References
1] William Nilsson (2005) Direct submission
2] Nilsson WB, Gudkovs N, Strom MS. (2006) Dis Aquat Organ. 23;70(3):209-17.