ISAzo6

  • Family IS1634
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Azoarcus sp. EbN1
DNA section
IS Length : 1925 bp

Ends


IR Length : 16

IRL : CCGGAGTTCGACAGTTCAGCCCGAAAAACGCCGTTTTTTGAAAACCCGCC
IRR : CCGGAGTTCGACAGTTAATCTCGTAACTATCTGATTTCGCTAGAGGCGAC

Insertion site


Left flankDirect repeatRight flankDR Length
GAAGACGATCTGGATTGGCCTGCAGC6

DNA sequence

CCGGAGTTCGACAGTTCAGCCCGAAAAACGCCGTTTTTTGAAAACCCGCCTCAGCATTCATGCGGGTTTCAGCCCGATTTTTCCGAAACTCTTGTGGTGG
CACGTTTGTAACAGCTACGACCTTGCTTGACGATGTATGCCGTGCTGCTAAAGTGGCGGCATGTTCGTCAAGATCACCACCTCCGGCGGCCGCCGCTACC
TTCAGCTCGTCCAGTCCTATCGGGATGATGCCGGCCGGGTGAAGAAGCGTACCGTGGCAACGCTTGGTCGGCTCGACCAGGTCGGCGGTGAGCTGGATTC
CGTGATCAACGGACTGCTCAGGGTCTCCGGCCGAGAACCCATGGGTGAGCGCCCTGCACCCCCTTCGGTCGCGTTCGAATCGGCCCGCGCACTGGGCGAC
GTCTGGGCGCTGACCGAGCTGTGGAAGGAGTTGGGCTTCGGGGCGCTGCGCCGGGTGTTTCGCCGTACGCGCCACACGACCGATGTCGAGGCGCTCATCC
GCGTGATGGTGCTCAACCGCCTGTGCGATCCGGACTCCAAGCTCGGCGTGCTGCGCTGGCTCGAGACGGTCGCGCTGCCCGAGGTCGACGTCAAGGCGAT
CACGCATCAGCAATTGCTGCGCAGCATGGATGCGCTGATGGCTCACCAGGCGGCGGTCGATGCGGTGGTGACGGGACTCCTGCGCCCGCTCGTCGATCAG
GACCTGTCGGTCGTCTTCTACGATCTCACCACGATCCGCGCCGAGGGGCTCACGAACGTGGCCGGCGACGTACGCGCCTTCGGCATGGCCAAAGAGGGGT
TGATTGCCCGCCAGTTCATGCTTGGCGTGGTGCAGACGGCCGACGGGCTTCCGATCTATCACGAGGTGTTCGCGGGCAACGCGGCCGAGACGACGACGCT
CCTGCCCACCCTCACGACGGTGCTCGAACGCTTCCCCGAAGTGCGCCGGCTTATCCTCGTGGCCGACCGCGGGTTGCTCAGCCTGGACAACCTCGAGGCG
CTCGAGGCGGTGCGGCTCGCGAGCGGTGAGCCGCTCGAGTTCATCATCGCGGTGCCGGGGCGGCGCTATCACGAGTTCGCCGAGCTCCTCGGCCCCTTCC
AGCGCACGCACTGCACGCAGGCGAGGGCCGAGGTGACCGGCGAACTCGCTTGGCAGGGGCTGCGGCTCATCGTCGCCCACGCTCCGGCGACAGCCGCGGT
GCAGAGCACACGGCGCAACGGCGAGATCGCCGCGCTCATCGCGCAGGGCGAAGCCTGGGCCAGCAAGCTCGTCGAGCAAGACGGCGGGGTCAAGCACCGC
GGCCGCAAACTCTCGGACAGCGGGGCCAAGGCGCGCTTCTACCACGCGGTGTGCGAGGCGCATCTGTCCAAAATCATCCGCGTCGACCTCAAGGCCGAGC
TCTTCCGCTACGAAATCGACGAGACGGCCCGGCGGCTTGCCGAACTCATGGACGGCAAGCTCCTGCTCGTGACCAACACGAAGGATCTCGCCCCTGCCGA
CGTCGTCACGCGCTACAAGGCGCTCGCCGACATCGAGCGCGGCTTCAAGGTGCTCAAATCCGAGATCGAGATCGGGCCGGTCTATCACCGGTTGCCCGAA
CGCATCCGCGCACACGCCTCGATCTGCTTCATGGCGCTGATCCTGCACCGCATCATGCGCGCGCGGCTGCGTGCGGCGCACACCGGGCTCACCCCCGAGC
GGGCGCTCGAGCAGCTGCATCGCATCCAGCACCACCGCGTCCGGCTCGGCGCCGCCGAACCTGTGACGGGGGTGTCTTCCATCAACGCACACCAGAACGA
GGTCCTCAGTGCCCTGCGGGTGAAAAAACCGGTCGCGCCTGAGCAGCTGACGCTGTTGTAGGGGCACGTTTGAAAGTCGCCTCTAGCGAAATCAGATAGT
TACGAGATTAACTGTCGAACTCCGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1701 bp566 aa1611861+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MFVKITTSGGRRYLQLVQSYRDDAGRVKKRTVATLGRLDQVGGELDSVINGLLRVSGREPMGERPAPPSVAFESARALGDVWALTELWKELGFGALRRVF
RRTRHTTDVEALIRVMVLNRLCDPDSKLGVLRWLETVALPEVDVKAITHQQLLRSMDALMAHQAAVDAVVTGLLRPLVDQDLSVVFYDLTTIRAEGLTNV
AGDVRAFGMAKEGLIARQFMLGVVQTADGLPIYHEVFAGNAAETTTLLPTLTTVLERFPEVRRLILVADRGLLSLDNLEALEAVRLASGEPLEFIIAVPG
RRYHEFAELLGPFQRTHCTQARAEVTGELAWQGLRLIVAHAPATAAVQSTRRNGEIAALIAQGEAWASKLVEQDGGVKHRGRKLSDSGAKARFYHAVCEA
HLSKIIRVDLKAELFRYEIDETARRLAELMDGKLLLVTNTKDLAPADVVTRYKALADIERGFKVLKSEIEIGPVYHRLPERIRAHASICFMALILHRIMR
ARLRAAHTGLTPERALEQLHRIQHHRVRLGAAEPVTGVSSINAHQNEVLSALRVKKPVAPEQLTLL

 

Blast result :
Comments
ISAzo6 is 43% aa similar to ISMyca1. There are 1 copy in Azoarcus sp. EbN1 genome and two copies in plasmid 2.
References
1] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36