ISAzo5
- Family IS4
- Group IS50
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006513 | ND | Azoarcus sp. | Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1 |
DNA section
IS Length : 1478 bp
Ends
IR Length : 19/20
IRL : CATCTCGTTATACACAACTCCGCACCGCCGGCACGCCGATCTGAATAATC
IRR : CATGTCGTTATACACAACTCAGGCCGCCTCATCGCGTAGCGCCTGTATGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTTCTACGC | ACTTTGACCG | 0 | |
| AGCCATGGTA | GCTTGAGCC | AGGTGGAATA | 9 |
| CATCGGACGG | CGCTGAGCC | CCGTTCCGGA | 9 |
| ACCGCATCGA | GGCAGAAGT | CGGTGGTCAG | 9 |
| ACCGCATCGA | GGCAGAAGT | CGGTGGTCAG | 9 |
| ATCGTCCTCG | ACCTTTCCT | GCGAACATCG | 9 |
| TTATACACGG | ACTTAAACC | AACGGACCAA | 9 |
| CAAGCGCAAT | GGCTAAGGA | GGCTTTGCAA | 9 |
DNA sequence
CATCTCGTTATACACAACTCCGCACCGCCGGCACGCCGATCTGAATAATCGTTGACCTTCAATGACTTACACGAAGTGCTTGTAAACCCATATAAAAACG
CGTCTACTTTTGCAATTTGGGTGACCTGTTGGCGACGAAGAGCTGGGTGGTGGAGGAGTTTGCGGGGATGGCGCTGGGCGACGAGAGACTGAACAAGCGG
GCGAAGGTGCTGATGGAGCGGTTGTCGGCGAAGCCGACGGCGGGCATTCCGATGGCGTGCCGGGGCTGGGGCGAGACGGTTGGCGCGTACCGCTTCCTGG
GCAACGACGAGGTCGACTGGCAGGCGATCCTGGCGCCGCATTGGGAGCGCACCCGCAGCCGCATGCGTGCCCATCCGGTGGTGTTGTGCCTGCAGGACAC
GACCGAGCTGGACTTCAACGGGCAGGGCATCGCGGGTCTGGGGCGGCTGAACTACGAGGCGCGCCGCGGCATGTACCTGCACCCGACCTATGCGGTGACG
CCCGAGCGCGAACCGCTGGGCGTGCTCGATGCGTGGATGTGGGCGCGCGAGGATGTCGATGAACCAGGCGAGCGCACCAGCATCAAGGAGAGCGCGCGCT
GGCCCGAAGGCTACGAGCGGGTCGCGGAGTTGGCCACGGGGATGGCGACCACCCGCCTGGTGTATGTAGCCGACCGCGAAGCGGATCTGATGGAAATGAT
GCTGCGCGCCCAGACGCTGGGCACGCCGGCCGACTGGCTGGTGCGGGCCAAGCACAACCGTTGCTTGCCAGAGGGCGCGAAGCTGTGGTCGCACACCTGC
GCAGGCGAGCCGCTGGGCGAGATCGTGTTCACGATGGGCTCGCGCCACGGCCAGAAGGCGCGCGAGGTGCGCCAGCAACTGTGGGCCCGACGGGTCAGCC
TGCCTGCGGGCAAGGCGGGCACGGTATCGGCCACCTGCATCGTGGCGCGCGAGATCGGCGCGCCGGCCGGCACCAAGCCCGTGGAGTGGCGCCTGCTCAC
CAACCGGGAAGCTGCGACGGCTGCCGACGTGGTCGAGCTGATCGACTGGTATCGGGCGCGCTGGGAGATCGAGACTTTCTTTCACGTGCTCAAGAACGGC
TGTCGGATCGAAGCGCTGCAACTGTCCGCCATCGAGCGCCTGGAGCGCGCGCTGGCGTTGTTCATGGTCGTCGCCTGGCGCATCGCGCATCTGATGCGCA
CCGGCCGAACCTGCCCCGAGCTGGATGCCACGCTCTTCTTCGACCCCGACGAGATCCGCGGCGCGTATCTGCTCACGAAGAAGAAACCGCCCGCCACCCC
GCCCACCATCAACGAAGTGCTGCGCCTGATCGCCACCCTCGGCGGCTTCCTGGCGCGCAAGGGCGACGGCGAGCCGGGTGTGAAGACCATCTGGCTGGGC
TTGCAGCGCGTCATGGATGCCGCCGCCACCATACAGGCGCTACGCGATGAGGCGGCCTGAGTTGTGTATAACGACATG
CGTCTACTTTTGCAATTTGGGTGACCTGTTGGCGACGAAGAGCTGGGTGGTGGAGGAGTTTGCGGGGATGGCGCTGGGCGACGAGAGACTGAACAAGCGG
GCGAAGGTGCTGATGGAGCGGTTGTCGGCGAAGCCGACGGCGGGCATTCCGATGGCGTGCCGGGGCTGGGGCGAGACGGTTGGCGCGTACCGCTTCCTGG
GCAACGACGAGGTCGACTGGCAGGCGATCCTGGCGCCGCATTGGGAGCGCACCCGCAGCCGCATGCGTGCCCATCCGGTGGTGTTGTGCCTGCAGGACAC
GACCGAGCTGGACTTCAACGGGCAGGGCATCGCGGGTCTGGGGCGGCTGAACTACGAGGCGCGCCGCGGCATGTACCTGCACCCGACCTATGCGGTGACG
CCCGAGCGCGAACCGCTGGGCGTGCTCGATGCGTGGATGTGGGCGCGCGAGGATGTCGATGAACCAGGCGAGCGCACCAGCATCAAGGAGAGCGCGCGCT
GGCCCGAAGGCTACGAGCGGGTCGCGGAGTTGGCCACGGGGATGGCGACCACCCGCCTGGTGTATGTAGCCGACCGCGAAGCGGATCTGATGGAAATGAT
GCTGCGCGCCCAGACGCTGGGCACGCCGGCCGACTGGCTGGTGCGGGCCAAGCACAACCGTTGCTTGCCAGAGGGCGCGAAGCTGTGGTCGCACACCTGC
GCAGGCGAGCCGCTGGGCGAGATCGTGTTCACGATGGGCTCGCGCCACGGCCAGAAGGCGCGCGAGGTGCGCCAGCAACTGTGGGCCCGACGGGTCAGCC
TGCCTGCGGGCAAGGCGGGCACGGTATCGGCCACCTGCATCGTGGCGCGCGAGATCGGCGCGCCGGCCGGCACCAAGCCCGTGGAGTGGCGCCTGCTCAC
CAACCGGGAAGCTGCGACGGCTGCCGACGTGGTCGAGCTGATCGACTGGTATCGGGCGCGCTGGGAGATCGAGACTTTCTTTCACGTGCTCAAGAACGGC
TGTCGGATCGAAGCGCTGCAACTGTCCGCCATCGAGCGCCTGGAGCGCGCGCTGGCGTTGTTCATGGTCGTCGCCTGGCGCATCGCGCATCTGATGCGCA
CCGGCCGAACCTGCCCCGAGCTGGATGCCACGCTCTTCTTCGACCCCGACGAGATCCGCGGCGCGTATCTGCTCACGAAGAAGAAACCGCCCGCCACCCC
GCCCACCATCAACGAAGTGCTGCGCCTGATCGCCACCCTCGGCGGCTTCCTGGCGCGCAAGGGCGACGGCGAGCCGGGTGTGAAGACCATCTGGCTGGGC
TTGCAGCGCGTCATGGATGCCGCCGCCACCATACAGGCGCTACGCGATGAGGCGGCCTGAGTTGTGTATAACGACATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1293 bp | 430 aa | 168 | 1460 | + | No |
Chemistry : DDE
ORF sequence :
MALGDERLNKRAKVLMERLSAKPTAGIPMACRGWGETVGAYRFLGNDEVDWQAILAPHWERTRSRMRAHPVVLCLQDTTELDFNGQGIAGLGRLNYEARR
GMYLHPTYAVTPEREPLGVLDAWMWAREDVDEPGERTSIKESARWPEGYERVAELATGMATTRLVYVADREADLMEMMLRAQTLGTPADWLVRAKHNRCL
PEGAKLWSHTCAGEPLGEIVFTMGSRHGQKAREVRQQLWARRVSLPAGKAGTVSATCIVAREIGAPAGTKPVEWRLLTNREAATAADVVELIDWYRARWE
IETFFHVLKNGCRIEALQLSAIERLERALALFMVVAWRIAHLMRTGRTCPELDATLFFDPDEIRGAYLLTKKKPPATPPTINEVLRLIATLGGFLARKGD
GEPGVKTIWLGLQRVMDAAATIQALRDEAA
GMYLHPTYAVTPEREPLGVLDAWMWAREDVDEPGERTSIKESARWPEGYERVAELATGMATTRLVYVADREADLMEMMLRAQTLGTPADWLVRAKHNRCL
PEGAKLWSHTCAGEPLGEIVFTMGSRHGQKAREVRQQLWARRVSLPAGKAGTVSATCIVAREIGAPAGTKPVEWRLLTNREAATAADVVELIDWYRARWE
IETFFHVLKNGCRIEALQLSAIERLERALALFMVVAWRIAHLMRTGRTCPELDATLFFDPDEIRGAYLLTKKKPPATPPTINEVLRLIATLGGFLARKGD
GEPGVKTIWLGLQRVMDAAATIQALRDEAA
Blast result :
Comments
ISAzo5 is 41% aa simialr to IS50.
There are 8 copies in Azoarcus sp. EbN1 genome.
There are 8 copies in Azoarcus sp. EbN1 genome.
References
1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation
2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.
3] PROSCIENCE (2004) Direct Submission GenBank.
4] ISfinder annotation