ISAzo3

  • Family ISAs1
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006513 ND Azoarcus sp.
Azoarcus sp. EbN1
DNA section
IS Length : 1282 bp

Ends


IR Length : 14/15

IRL : CAGGGCAATCGCACCTAAAATGCATAACCGATACAGGTAAACCGTTTACG
IRR : CAGGGCAATTGCACCGTCTTGTCATAACCCGAGGCGCTCAAATGGCCAAC

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

CAGGGCAATCGCACCTAAAATGCATAACCGATACAGGTAAACCGTTTACGATCATGCGCTTAGCGAGCCCCTGTTCACAACGTCATATTTTGTGTAGCTT
ACTGTCTTTTTGGGACGCCCATGAAGACAGGCCAGTTGATGCCCGTGAGCGAAGTGTTCGTGTCGGTACCCGACCCGCGCAGCAAGCGTCAGGCCCGGCA
CGATCTGTCCGAGTTGCTGACGGTGGCGGTGTGCGCGGTGCTGTGCGGGGCGAACGATTTCGTGGATGTGGCGCTGTGGGGCAAGTCCAACCTGGCCTGG
CTGCGCAAGTTCCTGAAGCTCAAGGCGGGCGTGCCCTCGCATGACACGTTCTGCCGGGTGCTCGCGATGATCGATCCCGCGGCCTTCGAGGCGGCCTTTC
TGCGCTGGGTGGGCGTGCTGGTGCCGGCGCTGGCGCCGGACAGCGTGGTGGCCATCGATGGCAAGACCAGTCGGCGCAGTGGCGGCAAGGATACGTCGGG
GCCGTTGCACATGGTCAGCGCCTTTGCCGCCGGGATGGGTCTGGTGCTGGGGCAGCGCGCCATCGATCAGAAGAGCAACGAGATCACGGCGATTCCCGAG
TTGCTCGCCATGCTGGCGCTCGAAGGCACCATCGTCACGATCGATGCGATGGGCACCCAGGCAGCGATTGCCCGCACCATCCGTAGTCGTGGCGCAGACT
ACGTGTTGTGCGTCAAAGACAACCACCCCACGCTGACCGACTCGATTCTGCTCACCCTGGCCGGCGTGGCGGAAAAGATCGCGCCGGCTTCGCATTTCGA
AGAACAAACCAAGGGCCACGGCCGCGTGGAGGTGCGCCGCTGCTGGGCCTATGATGCGGTCAGCCAGTTGTACAAGTCCGAGCAGTGGGCCGGGCTGCAA
TCGTTTGCGCTCGTCGAGCGCGAACGCACCGTCGACGGCAAGACCAGCGTCGAGCGCCATTACTACATCAGTTCCCTGCCCGCAGATGCCGCCAGAATCG
CGCAGGCCGTGCGCAGCCACTGGGCGGTCGAAAATCAGCTTCACTGGTCGCTCGATGTGCAGTTCAACGATGATCAGTCCCGTGTGCGCCGAGGCTATGC
GGCCAACAACTTCGTGGTGCTGCGCCACATCGTACTGAACCTGCTGCGCCACAACACCACCCGCAAGGCCAGCATCAAGTCCAAGCGACTGCTCGCCTGC
ATGGAGGATGACTTTCGCGAAGAGTTACTTGGGTTGGCCATTTGAGCGCCTCGGGTTATGACAAGACGGTGCAATTGCCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1125 bp374 aa1211245+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MKTGQLMPVSEVFVSVPDPRSKRQARHDLSELLTVAVCAVLCGANDFVDVALWGKSNLAWLRKFLKLKAGVPSHDTFCRVLAMIDPAAFEAAFLRWVGVL
VPALAPDSVVAIDGKTSRRSGGKDTSGPLHMVSAFAAGMGLVLGQRAIDQKSNEITAIPELLAMLALEGTIVTIDAMGTQAAIARTIRSRGADYVLCVKD
NHPTLTDSILLTLAGVAEKIAPASHFEEQTKGHGRVEVRRCWAYDAVSQLYKSEQWAGLQSFALVERERTVDGKTSVERHYYISSLPADAARIAQAVRSH
WAVENQLHWSLDVQFNDDQSRVRRGYAANNFVVLRHIVLNLLRHNTTRKASIKSKRLLACMEDDFREELLGLAI

 

Blast result :
Comments
ISAzo3 is 55% aa simialr to IS1358. There are 7 full copies and 3 partial copies in Azoarcus sp. EbN1 genome.
References
1] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36