LOCUS Exported 4837 bp ds-DNA linear BCT 03-JUN-2020 DEFINITION Bacillus thuringiensis serovar morrisoni strain EG2158 transposon Tn5401 ORF2, ORF1, site-specific recombinase (tnpI), and transposase (tnpA) genes, complete cds. ACCESSION U03554 VERSION . KEYWORDS Tn5401-U03554.1 SOURCE Bacillus thuringiensis serovar morrisoni ORGANISM Bacillus thuringiensis serovar morrisoni REFERENCE 1 (bases 1 to 4837) AUTHORS Baum JA. TITLE Tn5401, a new class II transposable element from Bacillus thuringiensis JOURNAL J. Bacteriol. 176 (10), 2835-2845 (1994) PUBMED 7514590 REFERENCE 2 (bases 1 to 4837) AUTHORS Baum JA. TITLE Direct Submission JOURNAL Submitted (17-NOV-1993) Jim A. Baum, Ecogen Inc., Strain Development/Molecular Genetics, 2005 Cabot Boulevard West, Langhorne, PA 19047-1810, USA REFERENCE 3 (bases 1 to 4837) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Jun 10, 2020 from SnapGene 5.1.3 https://www.snapgene.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Bacteriol."; date: "1994"; volume: "176"; issue: "10"; pages: "2835-2845" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (17-NOV-1993) Jim A. Baum, Ecogen Inc., Strain Development/Molecular Genetics, 2005 Cabot Boulevard West, Langhorne, PA 19047-1810, USA" FEATURES Location/Qualifiers source 1..4837 /organism="Bacillus thuringiensis serovar morrisoni" /strain="EG2158" /mol_type="genomic DNA" /serovar="morrisoni" /clone="pEG911-1" /db_xref="taxon:1441" mobile_element 1..4837 /mobile_element_type="transposon:Tn5401" /label=Tn5401 /note="Accession = Tn5401-U03554.1; Family = Tn3; Group = Tn3; Synonyms = ; Partial = ; Transposition = ; OtherInformation = unusually long 53-bp TIRs contain adjacent recognition sites for TnpA and TnpI|| Consensus sequence for repeat element ATGTCCRCTAAY || Inverted repeats nt 639-666 are presumed to be the site of strand exchange during cointegrate resolution; Hosts: Organism = Bacillus thuringiensis serovar morrisoni EG2158|Taxonomy = |BacGroup = |MolecularSource = |Region = Kansas|Country = U.S.A.|OtherLocInfo = soybean grain dust|DateIdentified = 1994|First = yes; Capture = yes " repeat_region 1..53 /label=IRL (Tn5401) /note="Name = IRL; AssociatedElement = Tn5401; LibraryName = IRL (Tn5401); OtherInformation = ; Capture = yes " misc_feature complement(40..51) /label=res_site_d (Tn5401) /note="Name = res_site_d; AssociatedElement = Tn5401; LibraryName = res_site_d (Tn5401); Capture = yes" CDS complement(119..343) /codon_start=1 /transl_table=11 /gene="parE" /product="ParE" /function="unknown" /label=parE (Tn5401) /note="AssociatedElement = Tn5401; Class = Passenger Gene; Subclass = Toxin; SequenceFamily = ParE_toxin (Pfam:PF05016); Target = DNA gyrase; Chemistry = ; LibraryName = parE (Tn5401); OtherInformation = ; Capture = yes" /protein_id="AAA64591.1" /translation="MKFIAKQEKGIQKRIAEGLKGLLKIPPEGDIKSMKGYTELYRLRI GTFRILFEINHDEKVIYIQAIGNRGDIYK" CDS complement(351..608) /codon_start=1 /transl_table=11 /gene="parD-noDBD" /product="ParD-noDBD" /function="unknown" /label=parD-noDBD (Tn5401) /note="AssociatedElement = Tn5401; Class = Passenger Gene; Subclass = Antitoxin; SequenceFamily = parD (PDB:4Q2U); Target = ; Chemistry = ; LibraryName = parD (Tn5401); OtherInformation = no PBD ; Capture = yes" /protein_id="AAA64590.1" /translation="MAIRKDELYRLIDHLDQQDEKAAFDFLEFLVQRSRRKPKEWEKID MADPDHEPLSTQELEQLNSEEGYVSGEDAKREFGLQIDLP" misc_feature 639..737 /function="Recognition site for TnpI recombinase" xxxx /label=res (Tn5401) /note="Name =res; AssociatedElement = Tn5401; LibraryName = res (Tn5401);Capture = yes " misc_feature 639..650 /label=res_site_a (Tn5401) /note="Name =res_site_a; AssociatedElement = Tn5401; LibraryName = res_site_a (Tn5401);Capture = yes " misc_feature complement(655..666) /label=res_site_b (Tn5401) /note="Name = res_site_b; AssociatedElement = Tn5401; LibraryName = res_site_a (Tn5401); Capture = yes " misc_feature 688..694 /label=-35 /note="Baum et al 1999" misc_feature 691..702 /label=res_site_c (Tn5401) /note="Name = res_site_c; AssociatedElement = Tn5401; LibraryName = res_site_c (Tn5401); Capture = yes" misc_feature 711..716 /label=-10 /note="Baum et al 1999" misc_feature complement(718..723) /label=-10 /note="Baum et al 1999" misc_feature 726..737 /label=res_site_d (Tn5401) /note="Name = res_site_d; AssociatedElement = Tn5401; LibraryName = res_site_d (Tn5401); Capture = yes" misc_feature complement(741..746) /label=-35 /note="Baum et al 1999" CDS 764..1684 /codon_start=1 /transl_table=11 /gene="tnpI" /product="TnpI" /function="cointegrate resolution, transcriptional regulation" /label=tnpI (Tn5401) /note="AssociatedElement = Tn5401; Class = Accessory Gene; Subclass =Resolvase; Target = ; SequenceFamily = Tyrosine Site-Specific Recombinase;Chemistry = Tyrosine; LibraryName = tnpI (Tn5401); OtherInformation = belongs to the phage integrase family of site-specific recombinases; Capture = yes " /experiment="experimental evidence, no additional details recorded" /protein_id="AAA64588.1" /translation="MHSTKTISIQATSLISDFISSLSQEGDLHTKTLKEYTSDLKDFVF WFENVWGKHAEDTLFHPIEVTARTIARYRGHMQVTRLLKPSTINRRINSIKRYFDWAKQ KGLVQTNYSKSIKFVPTEKTSPKRMSDKEEAALMHAVEKYGTLRDRAMIIFMLHTGLRS MEVCDVQIEDVIMRKRGGYVVVRSGKRNKQREVPLNSTARCALEEHIRLSEISQSYLFP SSKTGKRLQERAIRHILQKYIRLAKLEGFSAHDLRHRFGYVMAERTPLHRLAQIMGHDN LNTTMIYVRATQEDLQGEVEKIAWN" gene 764..1684 /gene="tnpI" /label=tnpI CDS 1756..4773 /codon_start=1 /transl_table=11 /gene="tnpA" /product="TnpA" /function="cointegrate formation during transposition" /label=tnpA (Tn5401) /note="AssociatedElement= Tn5401; Class = Transposase; Subclass = ; SequenceFamily = ; Chemistry = DDE;Target = ; LibraryName = tnpA (Tn5401); OtherInformation =; Capture = yes" /experiment="experimental evidence, no additional details recorded" /protein_id="AAA64589.1" /translation="MPVDFLTPEQEEKYGCFCDTPTSEQLAKYFWLDDTDKELIWNRRG EHNQLGFAVQLGTVRFLGTFLSDPTNVPQSVITYMANQLHLDAQSFSRYRNKRSQWDQM QEIRSVYGYKNFTDKSTHWRFIRWLYARAWLYNERPSVLFDLATARCIEQKILLPGVSV LTRLVSTVRDRSAENIWKKLSSLPDNVQKKQLENLLQIDQKTKKTYLERLSNPPVPISV TGIKNTLIRLQELRQLNTENWDMSRIPSKRLQQFARHTVAVRSQAIARMPDQRRMAMLV AFAKMYTQSAQDDVIDIFDRYLTDLFAKTYRKEQKERLRTIKDLDKAARQLREACVILL EHTDPSVHPKTAVFEKISEKDLIQAVQIVDSLTYSPNQTLAYSGLLQHYGIIRKFLPLL MEEIELQATPAGLPILQAWNFVKEHGKSNKKRWKNAPLAGLNANWSKVVIDKDSGTVNH RAYTFWMLEQVLEALHRHDLYIVGSEKYGDLRAQLLQDEEWKSIRPSILRSLDWSIDSY ESLTPLKEELDKTYHQVIENWENNPAVQIDTFAGKERIVLTPLDKQPEPESLQKLKQQI HTMLPNIDIPQLLLEVNRWTGFMDGFRHISEAKSRINELPISICALLISQACNIGLRPL VQDGVPSLERDRLTWIEQNYFRAETLSESNAKLVDFHSQLQLAKMWGGGEIASADGLRF ITPVKSVHTGPNPKYFGSGRGVTYYNYTSDQFTGLHGLVIPGTIRDSLYLLQCVLEQNT NLQPKEIMTDTAGYSDIIFGLFGLLGYQFSPRLADISESRLWRFDANSDYSMLNNLSKS RIREELIHRHWEDMLRVAGSLKLNKINATHLIQALQYNGKPTMLGRAIGELGRLFKTRY LLLYLHDENYRRKILNQLNRGEARHSLARAVFYGKRGELHQSYREGQEEQLGALGLVVN AIIVWNTRYIESALQVLRNRGHTIDNDDISRLSPLGHKHINIVGRYSFVLPEEVKDGQL RTLTYEETNKKEPDSL" gene 1756..4773 /gene="tnpA" /label=tnpA repeat_region complement(4785..4837) /label=IRR (Tn5401) /note="Name = IRR; AssociatedElement = Tn5401;LibraryName = IRR (Tn5401); OtherInformation = ;Capture = yes" misc_feature 4787..4798 /label=res_site_d (Tn5401) /note="Name = res_site_d; AssociatedElement = Tn5401; LibraryName = res_site_d (Tn5401); Capture = yes" ORIGIN 1 ggggtatgtg tagcaatgga acagaatcac gcaacaagca ttagcggaca ttattcgcac 61 acaaaaaagg aaggttcttc gattcagaag acctttcttt taaaaatgca tgtttgcctt 121 atttatagat gtcaccacga tttccaattg cttgtatgta tatgactttc tcatcatgat 181 ttatttcaaa taaaattcga aaggttccaa tccgtaatcg atatagttct gtgtaacctt 241 tcatactttt aatatctcct tcaggaggaa tcttaagaag tcccttcaat ccttctgcaa 301 ttcttttttg aatccctttt tcttgctttg caataaattt caccgcggac ttatggtaaa 361 tcaatttgta gtccgaattc acgttttgcg tcctcccctg atacatatcc ttcttcactg 421 tttaactgtt ctaactcttg tgtagacagc ggttcatgat caggatctgc catatcaatt 481 ttttcccatt ctttaggttt tcttcttgac cgttgaacaa gaaattctaa aaagtcaaat 541 gctgcttttt catcttgttg atccaggtga tcaattaacc gatacaattc atctttacga 601 atagccatgt gttacaccta ctttcgagat agttttaaat gtccactaat taatattagt 661 ggacatgaag tgtgggaaaa taaatgtttg atgtccgcta acataattga taagattaaa 721 atatcatgtc cgctaatgta agtcaataaa agaggaggta tttatgcatt ccactaaaac 781 aatttctata caagcaacat ctttgatttc cgattttatt tctagcttat ctcaagaagg 841 agatttgcat acaaaaacac taaaagaata tacgagtgat ttaaaagatt ttgtattttg 901 gtttgaaaat gtgtggggaa aacatgctga ggatactctt tttcatccaa tagaagttac 961 cgctcgcact attgctcgat atcgagggca tatgcaagtt acaagattac taaaaccttc 1021 tacgattaac cggcgcatta attcaatcaa acgttatttt gactgggcta agcaaaaagg 1081 actggtacaa acaaattatt caaaatcaat taagtttgta ccaacagaaa aaacgagtcc 1141 caaacgcatg tcagataaag aagaagccgc tttaatgcat gccgttgaaa aatacggcac 1201 actacgtgac agggcaatga ttatttttat gcttcatact ggccttcgtt caatggaagt 1261 gtgtgatgtt caaatagagg atgttatcat gagaaaaaga ggcggctatg ttgttgttcg 1321 atctggaaaa cgaaataaac agagggaagt gcctttgaat agtacagctc gttgtgcact 1381 agaagaacat atcagattaa gtgagatttc acagagttat ttgtttcctt cttctaaaac 1441 aggaaaacgc ctacaagaaa gagcgatccg ccatattctt cagaagtata ttagacttgc 1501 aaagttagaa ggatttagtg cccatgattt aaggcatcgc tttggttatg tgatggctga 1561 acgcacacca ttacatcgtc ttgcacaaat tatgggacac gataacttga ataccacgat 1621 gatttatgta agagctacac aagaagattt acagggagaa gtagaaaaga ttgcctggaa 1681 ctaaagaatg cacattatcc tactcatttg gtcatgtgat acaaaataag aattgtaaca 1741 ggaggaacaa gggttatgcc tgtagatttt ttaacacctg aacaagaaga aaaatatggt 1801 tgtttttgtg acactccaac atcagagcag ttagcaaaat atttttggtt agatgataca 1861 gacaaagaac tgatatggaa tcgtcgtgga gagcataatc aacttggttt cgctgttcaa 1921 ttaggaaccg ttaggttctt aggaacattt ttatctgatc ctacaaatgt accacaatcg 1981 gttattacat atatggcaaa tcaacttcat ctagatgctc aaagcttttc tcgttatcga 2041 aataaacgaa gtcagtggga tcaaatgcaa gagatacgtt ctgtttatgg atataaaaac 2101 tttacagata aatcaacaca ttggcgattc atcagatggc tatatgcacg tgcttggcta 2161 tataatgaac ggccaagtgt cttatttgat ttagcaacag cacgatgtat cgaacaaaaa 2221 attttactac ctggtgtatc tgtattaaca aggctagtat caacggttcg tgatcgttca 2281 gcagaaaata tatggaaaaa gctctctagt cttccggata atgttcagaa aaaacaatta 2341 gaaaaccttc ttcagataga tcaaaaaaca aagaaaacgt atttagagcg tctaagtaat 2401 ccccctgttc cgattagtgt tacgggcatt aagaatacgc tgattcgttt acaagagctt 2461 cgtcaattga acactgaaaa ttgggatatg tctagaattc cttcgaaaag attacaacaa 2521 ttcgcgcgtc acacagtcgc tgttagatca caagcaattg ctagaatgcc cgatcaacga 2581 cgtatggcta tgttagttgc atttgctaaa atgtatacac aaagtgctca ggatgatgtc 2641 attgatattt ttgatagata tttaacagat ttatttgcta agacatatcg aaaggaacaa 2701 aaagaacgtc ttcgtacaat taaggattta gataaggcag cgcgccaatt acgggaagct 2761 tgtgtaatat tattagaaca tacggatcct tctgtccatc caaaaacggc agtgtttgaa 2821 aaaatttcag aaaaggattt aatacaagct gtccaaattg ttgattcact cacctattca 2881 ccaaatcaaa cactagccta ttcaggattg ttacaacatt atggcataat ccgaaaattt 2941 cttcctttac tcatggaaga aattgaatta caagcaacgc ctgctggatt acccatcttg 3001 caagcatgga attttgtaaa agagcatggg aaatccaata agaaaagatg gaaaaatgct 3061 cctcttgccg gtttgaatgc aaattggtct aaggttgtaa ttgataaaga ttccggaact 3121 gtaaatcatc gagcatatac gttttggatg ctcgaacaag tattagaagc tttgcaccga 3181 catgatctat atatagtagg aagtgaaaaa tatggggacc ttcgcgcaca attattacaa 3241 gacgaagaat ggaaaagtat tcgtcctagt attcttcgct cattagactg gtcaatagat 3301 tcttatgaat cattgacacc gttaaaagaa gagttagaca aaacttatca tcaagtcatt 3361 gagaattggg agaataatcc tgcggtgcaa atagacacat ttgcaggtaa agagagaatt 3421 gttttgacac ctttagacaa acaaccagaa cctgaatcac tacaaaaact aaaacaacaa 3481 atacatacga tgttgccaaa tatagatatt cctcaattat tactcgaagt aaatcgttgg 3541 acgggattta tggatggttt tcgacatatt agtgaggcta aatctagaat taacgagtta 3601 cctataagta tctgtgcatt gcttatatct caagcatgca atattgggtt aagaccttta 3661 gttcaagatg gggttccttc attagaacgt gatcgtctta catggattga acaaaattat 3721 tttcgtgcag aaacactttc agaatcaaac gcgaaacttg tagattttca tagccaatta 3781 cagctggcta aaatgtgggg tggtggagaa attgcttcag ctgatggatt acgtttcatc 3841 acaccagtaa aatccgtaca cactggtcca aatcctaaat atttcggttc tggtcgtggt 3901 gttacgtatt acaactatac gagcgatcaa tttaccggac tccacggttt ggtgattcca 3961 ggcacaattc gtgattcatt atacttactt caatgtgtgt tagaacaaaa tacgaactta 4021 cagccaaaag aaattatgac agatacagct gggtatagtg atattatttt tgggctcttt 4081 ggattattag gatatcaatt tagtcctcgt ttagctgata tcagtgaatc acgtctttgg 4141 cgttttgatg cgaactcaga ttatagcatg ttaaataatt tgtctaaaag tcgcattcgt 4201 gaagaactca tacatcgtca ttgggaagac atgcttcgtg ttgcgggatc tttgaaacta 4261 aataaaataa atgcaacaca tcttatccaa gcacttcagt ataatgggaa accaactatg 4321 ttagggcgag caattggaga attggggaga ctctttaaaa cacgttattt actcttatat 4381 ttacatgatg aaaattatcg tcgtaaaatt ttaaatcaac tcaatagagg ggaagcaagg 4441 catagtttag cgagggctgt attttacggc aaacgtggag aacttcatca atcctatcga 4501 gaaggacaag aagagcaatt aggtgcatta ggtttagtag taaatgcaat tattgtatgg 4561 aatacacgat atatagaatc tgcgttacaa gtactccgaa atcgcggtca tacaattgat 4621 aatgatgata tatctagact ttcaccatta ggccataaac acattaacat agtaggtcgg 4681 tattcatttg ttctcccaga agaagtaaaa gatgggcaat tacgtacact aacatatgaa 4741 gaaacaaaca aaaaggaacc tgattcttta taagaatagg ttcctaatgt ccgctaatgc 4801 ttgttgcgtg attttgttcc attgctacac atacccc //