LOCUS Exported 5818 bp ds-DNA linear BCT 26-MAY-2020 DEFINITION Tn5-U00004.1. ACCESSION U00004 VERSION . KEYWORDS . SOURCE Escherichia coli ORGANISM Escherichia coli REFERENCE 1 (bases 1 to 5818) AUTHORS Berg DE. TITLE Julian Davies and the discovery of kanamycin resistance transposon Tn5. JOURNAL J. Antibiot. 2017;70:339-346. PUBMED 27731334 REFERENCE 2 (bases 1 to 5818) AUTHORS Berg DE, Davies J, Allet B, Rochaix JD. TITLE Transposition of R factor genes to bacteriophage lambda. JOURNAL Proc. Natl. Acad. Sci. U.S.A. 1975;72:3628-32. PUBMED 1059152 REFERENCE 3 (bases 1 to 5818) AUTHORS Auerswald EA, Ludwig G, Schaller H. TITLE Structural analysis of Tn5 JOURNAL Cold Spring Harb. Symp. Quant. Biol. 45 (PT 1), 107-113 (1981) PUBMED 6271452 REFERENCE 4 (bases 1 to 5818) AUTHORS Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H. TITLE Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5 JOURNAL Gene 19 (3), 327-336 (1982) PUBMED 6295884 REFERENCE 5 (bases 1 to 5818) AUTHORS Mazodier P, Cossart P, Giraud E, Gasser F. TITLE Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes JOURNAL Nucleic Acids Res. 13 (1), 195-205 (1985) PUBMED 3889831 REFERENCE 6 (bases 1 to 5818) AUTHORS Johnson RC, Yin JC, Reznikoff WS. TITLE Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat JOURNAL Cell 30 (3), 873-882 (1982) PUBMED 6291786 REFERENCE 7 (bases 1 to 5818) AUTHORS Rothstein SJ, Jorgensen RA, Yin JC, Yong-di Z, Johnson RC, Reznikoff WS. TITLE Genetic organization of Tn5 JOURNAL Cold Spring Harb. Symp. Quant. Biol. 45 (PT 1), 99-105 (1981) PUBMED 6271497 REFERENCE 8 (bases 1 to 5818) AUTHORS Rothstein SJ, Reznikoff WS. TITLE The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology JOURNAL Cell 23 (1), 191-199 (1981) PUBMED 6260374 REFERENCE 9 (bases 1 to 5818) AUTHORS Johnson RC, Reznikoff WS. TITLE DNA sequences at the ends of transposon Tn5 required for transposition JOURNAL Nature 304 (5923), 280-282 (1983) PUBMED 6306482 REFERENCE 10 (bases 1 to 5818) AUTHORS Berg DE. TITLE Transposon Tn5 JOURNAL (in) Berg,D.E. and Howe,M. (Eds.); MOBILE DNA: 185-210; American Society for Microbiology Press, Washington, D.C. (1989) REFERENCE 11 (bases 1 to 5818) AUTHORS Bradbury AR. TITLE Direct Submission JOURNAL Submitted (04-OCT-1993) International School for Advanced Studies (SISSA), Via Beirut 2-4, Trieste 34013, Italy REFERENCE 12 (bases 1 to 5818) AUTHORS Hengen PN. TITLE Direct Submission JOURNAL Submitted (04-OCT-1993) Laboratory of Mathematical Biology, National Cancer Institute, Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201, USA REFERENCE 13 (bases 1 to 5818) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, May 26, 2020 from SnapGene 5.1.3 https://www.snapgene.com COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Antibiot."; date: "2017"; volume: "70"; pages: "339-346" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Proc. Natl. Acad. Sci. U.S.A."; date: "1975"; volume: "72"; pages: "3628-32" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: " Cold Spring Harb. Symp. Quant. Biol. 45 (PT 1), 107-113 (1981)" COMMENT SGRef: number: 4; type: "Journal Article"; journalName: "Gene"; date: "1982"; volume: "19"; issue: "3"; pages: "327-336" COMMENT SGRef: number: 5; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "1985"; volume: "13"; issue: "1"; pages: "195-205" COMMENT SGRef: number: 6; type: "Journal Article"; journalName: "Cell"; date: "1982"; volume: "30"; issue: "3"; pages: "873-882" COMMENT SGRef: number: 7; type: "Journal Article"; journalName: " Cold Spring Harb. Symp. Quant. Biol. 45 (PT 1), 99-105 (1981)" COMMENT SGRef: number: 8; type: "Journal Article"; journalName: "Cell"; date: "1981"; volume: "23"; issue: "1"; pages: "191-199" COMMENT SGRef: number: 9; type: "Journal Article"; journalName: "Nature"; date: "1983"; volume: "304"; issue: "5923"; pages: "280-282" COMMENT SGRef: number: 10; type: "Journal Article"; journalName: "(in) Berg,D.E. and Howe,M. (Eds.)"; volume: " MOBILE DNA"; pages: " 185-210; American Society for Microbiology Press, Washington, D.C. (1989" COMMENT SGRef: number: 11; type: "Journal Article"; journalName: " Submitted (04-OCT-1993) International School for Advanced Studies (SISSA), Via Beirut 2-4, Trieste 34013, Italy" COMMENT SGRef: number: 12; type: "Journal Article"; journalName: " Submitted (04-OCT-1993) Laboratory of Mathematical Biology, National Cancer Institute, Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201, USA" COMMENT On May 17, 1994 this sequence version replaced gi:310980. FEATURES Location/Qualifiers source 1..5818 /organism="Escherichia coli" /mol_type="genomic DNA" /db_xref="taxon:562" mobile_element 1..5818 /mobile_element_type="transposon:Tn5" /label=Tn5 /note="Accession = Tn5-U00004.1; Family = Compound Transposon; Group = IS50; Synonyms = ; Partial = ; Transposition = yes; OtherInformation = ; Hosts:Organism = Pseudomonas aeruginosa|Taxonomy = |BacGroup = |MolecularSource = plasmid JR67|Region = |Country = |OtherLocInfo = clinical isolate from Schering-Plough/Upjohn|DateIdentified = 1975|First = yes; Capture= yes" mobile_element 1..1533 /mobile_element_type="insertion sequence:IS50L" /label=IS50L /note="Accession = IS50L-U00004.1; Family = IS4; Group = IS50; Synonyms =; Partial = ; Transposition = ; Otherinformation = ; Capture = yes" repeat_region 1..9 /label=IRL (IS50L) /note="Name = IRL; AssociatedElement = IS50L; LibraryName = IRL (IS50); OtherInformation=; Capture = yes" misc_feature 8..16 /label=putative DnaA box /note="putative DnaA box" CDS 92..1444 /codon_start=1 /gene="tnp_truncated" /product="Tnp_truncated" /label=tnp_truncated (IS50L) /note="AssociatedElement = IS50L; Class = Transposase; Subclass = ; Target = ; SequenceFamily = ; Chemistry = DDE ; Target = ; LibraryName = tnp_truncated (IS50L);OtherInformation = truncated to 421 amino acids by ochre mutation in C-terminal region which creates a promoter for aphA2 expression||non-functional; Capture= yes" /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS ITISSEGSEAMQEGAYRFYRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDRLAHNERFVVRSKHPRKDVESGL YLIDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA VLAEEINPPKGETPLKWLLLTGEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW" CDS 257..1444 /codon_start=1 /gene="inh_truncated" /product="Inh_truncated" /label=inh_truncated (IS50L) /note="AssociatedElement = IS50L; Class = Accessory Gene; Subclass = Inhibitor; SequenceFamily = ; Chemistry = ; Target = ; LibraryName = inh_truncated (IS50L); OtherInformation = truncated by ochre mutation in C-terminal end; Capture= yes" /translation="MQEGAYRFYRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTS LSYRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADE KESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDRLAHNERFVVRSKHPRKDV ESGLYLIDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNI TLNAVLAEEINPPKGETPLKWLLLTGEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGA GAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAET VLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALW" regulatory 1413..1462 /regulatory_class="promoter" /label=aphA2 /note="aminoglycoside phosphotransferase promoter region" /experiment="experimental evidence, no additional details recorded" variation 1442 /replace="g" /note="renders IS50L inactive by prematurely terminating the transposase" misc_feature 1510..1533 /label=putative IHF binding site /note="putative IHF binding site" repeat_region complement(1525..1533) /label=IRR (IS50L) /note="Name = IRR; AssociatedElement = IS50L; LibraryName = IRR (IS50) ; OtherInformation=; Capture = yes" CDS 1551..2345 /codon_start=1 /transl_table=11 /gene="APH(3')-IIa (ARO:3002644)" /locus_tag="RCS69_PI0065" /product="APH(3')-IIa" /function="antibiotic inactivation (ARO:0001004)" /EC_number="2.7.1.95" /label=APH(3')-IIa (Tn5) /note="AssociatedElement = Tn5; Class = Passenger Gene; Subclass = Antibiotic Resistance; SequenceFamily = APH(3') (ARO:3000126); Target = aminoglycoside antibiotic (ARO:0000016); Chemistry = ; LibraryName = APH(3')-IIa (Tn5); OtherInformation = ; Capture = yes" /inference="ab initio prediction:same species:2.0" /protein_id="SPE02301.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" CDS 2366..2746 /codon_start=1 /transl_table=11 /gene="BRP(MBL) (ARO:3001205)" /product="BRP(MBL)" /function="antibiotic inactivation (ARO:0001004)" /label=BRP(MBL) (Tn5) /note="AssociatedElement = Tn5; Class = Passenger Gene; Subclass = Antibiotic Resistance; SequenceFamily = Bleomycin resistant protein (ARO:3004256); Target = glycopeptide antibiotic (ARO:3000081); Chemistry = ; LibraryName = BRP(MBL) (Tn5); OtherInformation = loose match 57% to reference sequence for ARO:3001205 (bitscore: 142)||Synonyms: blmS, ble, BRP, blmA, BLMT; Capture = yes" /experiment="experimental evidence, no additional details recorded" /protein_id="AAA73391.1" /translation="MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLML EFFAHPGLDPLASWFSCCLRLDDLAEFYRQCKSVGIQETSSGYPRIHAPELQEWGGTMA ALVDPDGTLLRLIQNELLAGIS" CDS 2785..3585 /codon_start=1 /transl_table=11 /gene="APH(6)-Ic (ARO:3002659)" /product="APH(6)-Ic" /function="antibiotic inactivation (ARO:0001004)" /label=APH(6)-Ic (Tn5) /note="AssociatedElement = Tn5; Class = Passenger Gene; Subclass = Antibiotic Resistance; SequenceFamily = APH(6) (ARO:3000151); Target = aminoglycoside antibiotic (ARO:0000016); Chemistry = ; LibraryName = APH(6)-Ic (Tn5); OtherInformation = perfect match to ARO:3002659 reference sequence||Synonym: str; Capture = yes" /experiment="experimental evidence, no additional details recorded" /protein_id="AAA73392.1" /translation="MERWRLLRDGELLTTHSSWILPVRQGDMPAMLKVARIPDEEAGYR LLTWWDGQGAARVFASAAGALLMERASGAGDLAQIAWSGQDDEACRILCDTAARLHAPR SGPPPDLHPLQEWFQPLFRLAAEHAALAPAASVARQLLAAPREVCPLHGDLHHENVLDF GDRGWLAIDPHGLLGERTFDYANIFTNPDLSDPGRPLAILPGRLEARLSIVVATTGFEP ERLLRWIIAWTGLSAAWFIGDGDGEGEGAAIDLAVNAMARRLLD" mobile_element 4286..5818 /mobile_element_type="insertion sequence:IS50R" /label=IS50R /note="Accession = IS50R-U00004.1; Family = IS4; Group = IS50; Synonyms = ; Partial = ; Transposition = yes; Otherinformation = ; Capture = yes" misc_feature 4286..4309 /label=putative IHF binding site /note="putative IHF binding site" repeat_region 4286..4294 /label=IRR (IS50R) /note="Name = IRR; AssociatedElement = IS50R; LibraryName = IRR (IS50); OtherInformation=; Capture = yes" CDS complement(4297..5727) /codon_start=1 /gene="tnp" /product="Tnp" /label=tnp (IS50R) /note="AssociatedElement = IS50R; Class = Transposase; Subclass = ; Target = ; SequenceFamily = ; Chemistry = DDE; Target = ; LibraryName = tnp (IS50) ;OtherInformation = ; Capture= yes" /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS ITISSEGSEAMQEGAYRFYRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDRLAHNERFVVRSKHPRKDVESGL YLIDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA VLAEEINPPKGETPLKWLLLTGEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER QRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAETVLTP DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS KLDGFLAAKDLMAQGIKI" CDS complement(4298..5562) /codon_start=1 /gene="inh" /product="Inh" /function="Inhibitor of transposition" /label=inh (IS50R) /note="AssociatedElement = IS50R; Class = Accessory Gene; Subclass = Inhibitor; Target = ; SequenceFamily = ; Chemistry = ; Target = ; LibraryName = inh (IS50); OtherInformation = has an internal start in the transposase gene from an internal promoter; Capture= yes" /translation="MQEGAYRFYRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTS LSYRHQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADE KESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDRLAHNERFVVRSKHPRKDV ESGLYLIDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNI TLNAVLAEEINPPKGETPLKWLLLTGEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGA GAERQRMEEPDNLERMVSILSFVAVRLLQLRESFTLPQALRAQGLLKEAEHVESQSAET VLTPDECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWE ALQSKLDGFLAAKDLMAQGIKI" misc_feature 5803..5811 /label=putative DnaA box /note="putative DnaA box" repeat_region complement(5810..5818) /label=IRL (IS50R) /note="Name = IRL; AssociatedElement = IS50R; LibraryName = IRL (IS50); OtherInformation=; Capture = yes" ORIGIN 1 ctgactctta tacacaagta gcgtcctgaa cggaaccttt cccgttttcc aggatctgac 61 ttccatgtga cctcctaaca tggtaacgtt catgataact tctgctcttc atcgtgcggc 121 cgactgggct aaatctgtgt tctcttcggc ggcgctgggt gatcctcgcc gtactgcccg 181 cttggttaac gtcgccgccc aattggcaaa atattctggt aaatcaataa ccatctcatc 241 agagggtagt gaagccatgc aggaaggcgc ttaccgattt taccgcaatc ccaacgtttc 301 tgccgaggcg atcagaaagg ctggcgccat gcaaacagtc aagttggctc aggagtttcc 361 cgaactgctg gccattgagg acaccacctc tttgagttat cgccaccagg tcgccgaaga 421 gcttggcaag ctgggctcta ttcaggataa atcccgcgga tggtgggttc actccgttct 481 cttgctcgag gccaccacat tccgcaccgt aggattactg catcaggagt ggtggatgcg 541 cccggatgac cctgccgatg cggatgaaaa ggagagtggc aaatggctgg cagcggccgc 601 aactagccgg ttacgcatgg gcagcatgat gagcaacgtg attgcggtct gtgaccgcga 661 agccgatatt catgcttatc tgcaggacag gctggcgcat aacgagcgct tcgtggtgcg 721 ctccaagcac ccacgcaagg acgtagagtc tgggttgtat ctgatcgacc atctgaagaa 781 ccaaccggag ttgggtggct atcagatcag cattccgcaa aagggcgtgg tggataaacg 841 cggtaaacgt aaaaatcgac cagcccgcaa ggcgagcttg agcctgcgca gtgggcgcat 901 cacgctaaaa caggggaata tcacgctcaa cgcggtgctg gccgaggaga ttaacccgcc 961 caagggtgag accccgttga aatggttgtt gctgaccggc gaaccggtcg agtcgctagc 1021 ccaagccttg cgcgtcatcg acatttatac ccatcgctgg cggatcgagg agttccataa 1081 ggcatggaaa accggagcag gagccgagag gcaacgcatg gaggagccgg ataatctgga 1141 gcggatggtc tcgatcctct cgtttgttgc ggtcaggctg ttacagctca gagaaagctt 1201 cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag 1261 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa 1321 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc 1381 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg 1441 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat 1501 ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 1561 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 1621 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 1681 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 1741 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 1801 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 1861 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 1921 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 1981 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 2041 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 2101 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 2161 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 2221 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 2281 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 2341 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 2401 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 2461 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg 2521 gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt 2581 ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca 2641 tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc cggacgggac 2701 gctcctgcgc ctgatacaga acgaattgct tgcaggcatc tcatgagtgt gtcttcccgt 2761 tttccgcctg aggtcactgc gtggatggag cgctggcgcc tgctgcgcga cggcgagctg 2821 ctcaccaccc actcgagctg gatacttccc gtccgccagg gggacatgcc ggcgatgctg 2881 aaggtcgcgc gcattcccga tgaagaggcc ggttaccgcc tgttgacctg gtgggacggg 2941 cagggcgccg cccgagtctt cgcctcggcg gcgggcgctc tgctcatgga gcgcgcgtcc 3001 ggggccgggg accttgcaca gatagcgtgg tccggccagg acgacgaggc ttgcaggatc 3061 ctctgcgaca ccgccgctcg tctgcacgcg ccgcggtccg gaccgccgcc cgatctccat 3121 ccgctacagg aatggttcca gccgcttttc cggttggccg ctgagcacgc ggcacttgcg 3181 cccgccgcca gcgtagcgcg ccaacttctg gcggcgccgc gcgaggtgtg cccgctccac 3241 ggcgacctgc accacgagaa cgtgctcgac ttcggcgacc gcggctggct ggccatcgac 3301 ccgcacggac tgctcggcga gcgcaccttc gactatgcca acatcttcac gaatcccgat 3361 ctcagcgacc ccggtcgccc gcttgcgatc ctgccgggca ggctggaggc tcgactcagc 3421 attgtggtcg cgacgaccgg gtttgagccc gaacggcttc ttcgctggat cattgcatgg 3481 acgggcttgt cggcagcctg gttcatcggc gacggcgacg gcgagggcga gggcgctgcg 3541 attgatctgg ccgtaaacgc catggcacgc cggttgcttg actagcgcgg tcaccgatct 3601 cacctggtcg tcgagctagg tcaggccgtg tcgggcgtga tccgctggaa gtcgttgcgg 3661 gccacacccg ccgcctcgaa gccctgcacc aggccggcat cgtggtgtgc gtggccgagg 3721 gactatggaa ggtgccggac gatctgcccg agcagggccg ccgctatgac gcccagcgtc 3781 ttggtggcgt gacggtggag ctgaaatcgc acctgcccat cgagcggcag gcccgcgtga 3841 tcggtgccac ctggcttgac cagcagttga tcgacggtgg ctcgggcttg ggcgacctgg 3901 gctttagcag tgaggccaag taggcgatac agcagcgcgc ggacttcctg gccgaacagg 3961 gactggccga gcggcgcggg cagcgcgtga tcctcaccgg aatctgctcg gcagcagcgg 4021 gctcgggaac tggcgcaggc cgcgaaggac attgccgccg ataccggcct ggagcatcgc 4081 cccgtggccg acggccagcg cgttgccggc gtctaccggc gccccgtcat gctcgccagc 4141 gggcgaaatg ggatgcttga tgacgccaag gggtccagcc tcgtgcggtg gaagcccatc 4201 gaacagcggc ttggggagca gctcgccgcg acggtgcgcg gtggcggcgt gtcttgggag 4261 attggacgac agcgtgggcc ggcccctgtc tcttgatcag atcttgatcc cctgcgccat 4321 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttccca 4381 gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc 4441 tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt 4501 gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct 4561 ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa 4621 gctttctctg agctgtaaca gcctgaccgc aacaaacgag aggatcgaga ccatccgctc 4681 cagattatcc ggctcctcca tgcgttgcct ctcggctcct gctccggttt tccatgcctt 4741 atggaactcc tcgatccgcc agcgatgggt ataaatgtcg atgacgcgca aggcttgggc 4801 tagcgactcg accggttcgc cggtcagcaa caaccatttc aacggggtct cacccttggg 4861 cgggttaatc tcctcggcca gcaccgcgtt gagcgtgata ttcccctgtt ttagcgtgat 4921 gcgcccactg cgcaggctca agctcgcctt gcgggctggt cgatttttac gtttaccgcg 4981 tttatccacc acgccctttt gcggaatgct gatctgatag ccacccaact ccggttggtt 5041 cttcagatgg tcgatcagat acaacccaga ctctacgtcc ttgcgtgggt gcttggagcg 5101 caccacgaag cgctcgttat gcgccagcct gtcctgcaga taagcatgaa tatcggcttc 5161 gcggtcacag accgcaatca cgttgctcat catgctgccc atgcgtaacc ggctagttgc 5221 ggccgctgcc agccatttgc cactctcctt ttcatccgca tcggcagggt catccgggcg 5281 catccaccac tcctgatgca gtaatcctac ggtgcggaat gtggtggcct cgagcaagag 5341 aacggagtga acccaccatc cgcgggattt atcctgaata gagcccagct tgccaagctc 5401 ttcggcgacc tggtggcgat aactcaaaga ggtggtgtcc tcaatggcca gcagttcggg 5461 aaactcctga gccaacttga ctgtttgcat ggcgccagcc tttctgatcg cctcggcaga 5521 aacgttggga ttgcggtaaa atcggtaagc gccttcctgc atggcttcac taccctctga 5581 tgagatggtt attgatttac cagaatattt tgccaattgg gcggcgacgt taaccaagcg 5641 ggcagtacgg cgaggatcac ccagcgccgc cgaagagaac acagatttag cccagtcggc 5701 cgcacgatga agagcagaag ttatcatgaa cgttaccatg ttaggaggtc acatggaagt 5761 cagatcctgg aaaacgggaa aggttccgtt caggacgcta cttgtgtata agagtcag //