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Recombination Sites | |
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| Name: Tn903 |
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| Family: Compound Transposon Group: IS903 |
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| Evidence of Transposition: yes |
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| Host |
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| Host Organism: | Escherichia coli | Molecular Source: | plasmid R6-5 |
| | Date of Isolation: | 1978 |
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| Map |
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| Terminal Inverted Repeats (IR) |
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| IRR (Length: 18 bp) |  | GGCTTTGTTGAATAAATC |
| IRR (Length: 18 bp) | | GGCTTTGTTGAATAAATC |
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| Sequence |
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--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
GGCTTTGTTG AATAAATCAG ATTTCGGGTA AGTCTCCCCC GTAGCGGGTT GTGTTTTCAG GCAATACGCA CGCTTTCAGG CATACCTGCT TTCGTCATTT 100
TGTTCAGCGC TCGTACCAGG GCCATAGCCT CCGCAACCTG ACCATCGTAG TCACGCAGCG TCAGTGAACC CCCGAACAGC TGTTTTACCC GGTACATCGC 200
CGTTTCCGCT ATCGAGCGAC GGTTGTAATC TGTTGTCCAT TTCCACCGCG CATTACTCCC GGTCATTCGC TGATTAGCCA CTGCACGGTT ACGGTCTGCA 300
TATTCACCGG GCCAGTAACC CGCACCTTTT CGGGGAGGGA TAAGCGCGCT GATTTTCTTA CGCCGCAGTT CATCGTGACA GAGCCGGGTG TCGTAAGCGC 400
CGTCTGCCGA TGCTGCCCTG ATTTTTCTGT GAGTCTGCCG GATAAGACCC GGGAAGGCTT CTGAGTCGGT CACATTGTTC AGCGACAGGT CAGCGCAGAT 500
GATTTCATGT GTTTTACTGT CAACGGCGAG ATGCAGCTTA CGCCAGATAC GGCGGCGTTC CTGGCCATGC TTTTTGACTT TCCATTCGCC TTCACCAAAG 600
ACCTTCAGCC CGGTGGAATC AATCACCAGA TGCGCGATTT CACCCCGGGT GAACGTTTTG AAACTGATAT TAACCGACTT TGCCCGCCTG CTGACACAGC 700
TGTAATCCGG GCAGCGCAAC GGAACATTCA TCAGTGTAAA AATGGAATCA ATAAAGCCCT GCGCAGCGCG CAGGGTCAGC CTGAATACGC GTTTAATGAC 800
CAGCACAGTC GTGATGGCAA GGTCAGAATA GCGCTGAGGT CTGCCTCGTG AAGAAGGTGT TGCTGACTCA TACCAGGCCT GAATCGCCCC ATCATCCAGC 900
CAGAAAGTGA GGGAGCCACG GTTGATGAGA GCTTTGTTGT AGGTGGACCA GTTGGTGATT TTGAACTTTT GCTTTGCCAC GGAACGGTCT GCGTTGTCGG 1000
GAAGATGCGT GATCTGATCC TTCAACTCAG CAAAAGTTCG ATTTATTCAA CAAAGCCACG TTGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 1100
AAATATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCTTG CTCGAGGCCG 1200
CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGATTG TATGGGAAGC 1300
CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 1400
TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGG AAAACAGCAT TCCAGGTATT AGAAGAATAT 1500
CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT 1600
TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 1700
AGAAATGCAT AAGCTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT 1800
TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC 1900
TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 2000
ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGGC TTTGTTGAAT AAATCGAACT TTTGCTGAGT TGAAGGATCA GATCACGCAT CTTCCCGACA 2100
ACGCAGACCG TTCCGTGGCA AAGCAAAAGT TCAAAATCAC CAACTGGTCC ACCTACAACA AAGCTCTCAT CAACCGTGGC TCCCTCACTT TCTGGCTGGA 2200
TGATGGGGCG ATTCAGGCCT GGTATGAGTC AGCAACACCT TCTTCACGAG GCAGACCTCA GCGCTATTCT GACCTTGCCA TCACGACTGT GCTGGTCATT 2300
AAACGCGTAT TCAGGCTGAC CCTGCGCGCT GCGCAGGGCT TTATTGATTC CATTTTTACA CTGATGAATG TTCCGTTGCG CTGCCCGGAT TACAGCTGTG 2400
TCAGCAGGCG GGCAAAGTCG GTTAATATCA GTTTCAAAAC GTTCACCCGG GGTGAAATCG CGCATCTGGT GATTGATTCC ACCGGGCTGA AGGTCTTTGG 2500
TGAAGGCGAA TGGAAAGTCA AAAAGCATGG CCAGGAACGC CGCCGTATCT GGCGTAAGCT GCATCTCGCC GTTGACAGTA AAACACATGA AATCATCTGC 2600
GCTGACCTGT CGCTGAACAA TGTGACCGAC TCAGAAGCCT TCCCGGGTCT TATCCGGCAG ACTCACAGAA AAATCAGGGC AGCATCGGCA GACGGCGCTT 2700
ACGACACCCG GCTCTGTCAC GATGAACTGC GGCGTAAGAA AATCAGCGCG CTTATCCCTC CCCGAAAAGG TGCGGGTTAC TGGCCCGGTG AATATGCAGA 2800
CCGTAACCGT GCAGTGGCTA ATCAGCGAAT GACCGGGAGT AATGCGCGGT GGAAATGGAC AACAGATTAC AACCGTCGCT CGATAGCGGA AACGGCGATG 2900
TACCGGGTAA AACAGCTGTT CGGGGGTTCA CTGACGCTGC GTGACTACGA TGGTCAGGTT GCGGAGGCTA TGGCCCTGGT ACGAGCGCTG AACAAAATGA 3000
CGAAAGCAGG TATGCCTGAA AGCGTGCGTA TTGCCTGAAA ACACAACCCG CTACGGGGGA GACTTACCCG AAATCTGATT TATTCAACAA AGCC
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| ORFs |
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| Gene Name |
Associated TE |
Coordinates |
Class |
Sub Class |
Orientation |
| tnpA |
IS903 |
57-980 |
Transposase |
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- |
| APH(3')-Ia (ARO:3002641) |
Tn903 |
1162-1977 |
Passenger Gene |
Antibiotic Resistance |
+ |
| tnpA |
IS903 |
2115-3038 |
Transposase |
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+ |
| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnpA |
TnpA |
IS903 |
924 |
57-980 |
- |
| Class: | Transposase |
| Function: | GO molecular function: DNA binding, transposase activity. |
| Transpoase Chemistry: | DDE |
Protein Sequence:
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MAKQKFKITN WSTYNKALIN RGSLTFWLDD GAIQAWYESA TPSSRGRPQR YSDLAITTVL VIKRVFRLTL RAAQGFIDSI FTLMNVPLRC PDYSCVSRRA
KSVNISFKTF TRGEIAHLVI DSTGLKVFGE GEWKVKKHGQ ERRRIWRKLH LAVDSKTHEI ICADLSLNNV TDSEAFPGLI RQTHRKIRAA SADGAYDTRL
CHDELRRKKI SALIPPRKGA GYWPGEYADR NRAVANQRMT GSNARWKWTT DYNRRSIAET AMYRVKQLFG GSLTLRDYDG QVAEAMALVR ALNKMTKAGM
PESVRIA
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| APH(3')-Ia (ARO:3002641) |
APH(3')-Ia |
Tn903 |
816 |
1162-1977 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Antibiotic Resistance |
| Function: | antibiotic inactivation (ARO:0001004) |
| Target: | aminoglycoside antibiotic (ARO:0000016) |
| Sequence Family: | APH(3') (ARO:3000126) |
| Comment: | strict match to reference sequence for ARO:3002641 (bitscore: 560)||Synonyms: aphA-1, apha1-1AB, APH(3')-Ic, apha7 |
Protein Sequence:
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MSHIQRETSC SRPRLNSNMD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP ELFLKHGKGS VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA
IPGKTAFQVL EEYPDSGENI VDALAVFLRR LHSIPVCNCP FNSDRVFRLA QAQSRMNNGL VDASDFDDER NGWPVEQVWK EMHKLLPFSP DSVVTHGDFS
LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS PSLQKRLFQK YGIDNPDMNK LQFHLMLDEF F
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnpA |
TnpA |
IS903 |
924 |
2115-3038 |
+ |
| Class: | Transposase |
| Function: | GO molecular function: DNA binding, transposase activity. |
| Transpoase Chemistry: | DDE |
Protein Sequence:
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MAKQKFKITN WSTYNKALIN RGSLTFWLDD GAIQAWYESA TPSSRGRPQR YSDLAITTVL VIKRVFRLTL RAAQGFIDSI FTLMNVPLRC PDYSCVSRRA
KSVNISFKTF TRGEIAHLVI DSTGLKVFGE GEWKVKKHGQ ERRRIWRKLH LAVDSKTHEI ICADLSLNNV TDSEAFPGLI RQTHRKIRAA SADGAYDTRL
CHDELRRKKI SALIPPRKGA GYWPGEYADR NRAVANQRMT GSNARWKWTT DYNRRSIAET AMYRVKQLFG GSLTLRDYDG QVAEAMALVR ALNKMTKAGM
PESVRIA
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| Internal Transposable Elements (TE) |
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| TnCentral Accession |
TE Name |
Type |
Coordinates |
Length |
| IS903-V00359.1 |
IS903 |
Insertion Sequence |
1-1057 |
1057 |
| IS903-V00359.1 |
IS903 |
Insertion Sequence |
2038-3094 |
1057 |
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| Internal Repeat Elements |
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| Name |
Associated Mobile Element |
Coordinates |
Sequence (Top Strand) |
| IRL |
IS903 |
1040-1057 |
CTAAATAAGT TGTTTCGG |
| IRL |
IS903 |
2038-2055 |
GGCTTTGTTG AATAAATC |
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| References |
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| 1. | Hershfield V, Boyer HW, Chow L, Helinski DR. Characterization of a mini-ColC1 plasmid. J Bacteriol. 1976 Apr;126(1):447-53. doi: 10.1128/jb.126.1.447-453.1976. PubMed ID: 770430
| | | 2. | Oka A, Sugisaki H, Takanami M. Nucleotide sequence of the kanamycin resistance transposon Tn903. J Mol Biol. 1981 Apr 5;147(2):217-26. doi: 10.1016/0022-2836(81)90438-1. PubMed ID: 6270337
| | | 3. | Nomura N, Yamagishi H, Oka A. Isolation and characterization of transducing coliphage fd carrying a kanamycin resistance gene. Gene. 1978 Feb;3(1):39-51. doi: 10.1016/0378-1119(78)90006-9. PubMed ID: 344143
| | | 4. | Grindley ND, Joyce CM. Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7176-80. doi: 10.1073/pnas.77.12.7176. PubMed ID: 6261245
| | | 5. | Swingle B, O'Carroll M, Haniford D, Derbyshire KM. The effect of host-encoded nucleoid proteins on transposition: H-NS influences targeting of both IS903 and Tn10. Mol Microbiol. 2004 May;52(4):1055-67. doi: 10.1111/j.1365-2958.2004.04051.x. PubMed ID: 15130124
| | | 6. | Twiss E, Coros AM, Tavakoli NP, Derbyshire KM. Transposition is modulated by a diverse set of host factors in Escherichia coli and is stimulated by nutritional stress. Mol Microbiol. 2005 Sep;57(6):1593-607. doi: 10.1111/j.1365-2958.2005.04794.x. PubMed ID: 16135227
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