Transposon
Name: Tn903
Family: Compound Transposon        Group: IS903
Evidence of Transposition: yes
 Host     

Host Organism:Escherichia coli Molecular Source:plasmid R6-5
Date of Isolation:1978

 Map     



 Terminal Inverted Repeats (IR)     

IRR (Length: 18 bp)GGCTTTGTTGAATAAATC
IRR (Length: 18 bp)GGCTTTGTTGAATAAATC

 Sequence     
DNA SequenceLength  3094 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
GGCTTTGTTG AATAAATCAG ATTTCGGGTA AGTCTCCCCC GTAGCGGGTT GTGTTTTCAG GCAATACGCA CGCTTTCAGG CATACCTGCT TTCGTCATTT 100
TGTTCAGCGC TCGTACCAGG GCCATAGCCT CCGCAACCTG ACCATCGTAG TCACGCAGCG TCAGTGAACC CCCGAACAGC TGTTTTACCC GGTACATCGC 200
CGTTTCCGCT ATCGAGCGAC GGTTGTAATC TGTTGTCCAT TTCCACCGCG CATTACTCCC GGTCATTCGC TGATTAGCCA CTGCACGGTT ACGGTCTGCA 300
TATTCACCGG GCCAGTAACC CGCACCTTTT CGGGGAGGGA TAAGCGCGCT GATTTTCTTA CGCCGCAGTT CATCGTGACA GAGCCGGGTG TCGTAAGCGC 400
CGTCTGCCGA TGCTGCCCTG ATTTTTCTGT GAGTCTGCCG GATAAGACCC GGGAAGGCTT CTGAGTCGGT CACATTGTTC AGCGACAGGT CAGCGCAGAT 500
GATTTCATGT GTTTTACTGT CAACGGCGAG ATGCAGCTTA CGCCAGATAC GGCGGCGTTC CTGGCCATGC TTTTTGACTT TCCATTCGCC TTCACCAAAG 600
ACCTTCAGCC CGGTGGAATC AATCACCAGA TGCGCGATTT CACCCCGGGT GAACGTTTTG AAACTGATAT TAACCGACTT TGCCCGCCTG CTGACACAGC 700
TGTAATCCGG GCAGCGCAAC GGAACATTCA TCAGTGTAAA AATGGAATCA ATAAAGCCCT GCGCAGCGCG CAGGGTCAGC CTGAATACGC GTTTAATGAC 800
CAGCACAGTC GTGATGGCAA GGTCAGAATA GCGCTGAGGT CTGCCTCGTG AAGAAGGTGT TGCTGACTCA TACCAGGCCT GAATCGCCCC ATCATCCAGC 900
CAGAAAGTGA GGGAGCCACG GTTGATGAGA GCTTTGTTGT AGGTGGACCA GTTGGTGATT TTGAACTTTT GCTTTGCCAC GGAACGGTCT GCGTTGTCGG 1000
GAAGATGCGT GATCTGATCC TTCAACTCAG CAAAAGTTCG ATTTATTCAA CAAAGCCACG TTGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 1100
AAATATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCTTG CTCGAGGCCG 1200
CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGATTG TATGGGAAGC 1300
CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 1400
TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGG AAAACAGCAT TCCAGGTATT AGAAGAATAT 1500
CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT 1600
TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 1700
AGAAATGCAT AAGCTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT 1800
TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC 1900
TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 2000
ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGGC TTTGTTGAAT AAATCGAACT TTTGCTGAGT TGAAGGATCA GATCACGCAT CTTCCCGACA 2100
ACGCAGACCG TTCCGTGGCA AAGCAAAAGT TCAAAATCAC CAACTGGTCC ACCTACAACA AAGCTCTCAT CAACCGTGGC TCCCTCACTT TCTGGCTGGA 2200
TGATGGGGCG ATTCAGGCCT GGTATGAGTC AGCAACACCT TCTTCACGAG GCAGACCTCA GCGCTATTCT GACCTTGCCA TCACGACTGT GCTGGTCATT 2300
AAACGCGTAT TCAGGCTGAC CCTGCGCGCT GCGCAGGGCT TTATTGATTC CATTTTTACA CTGATGAATG TTCCGTTGCG CTGCCCGGAT TACAGCTGTG 2400
TCAGCAGGCG GGCAAAGTCG GTTAATATCA GTTTCAAAAC GTTCACCCGG GGTGAAATCG CGCATCTGGT GATTGATTCC ACCGGGCTGA AGGTCTTTGG 2500
TGAAGGCGAA TGGAAAGTCA AAAAGCATGG CCAGGAACGC CGCCGTATCT GGCGTAAGCT GCATCTCGCC GTTGACAGTA AAACACATGA AATCATCTGC 2600
GCTGACCTGT CGCTGAACAA TGTGACCGAC TCAGAAGCCT TCCCGGGTCT TATCCGGCAG ACTCACAGAA AAATCAGGGC AGCATCGGCA GACGGCGCTT 2700
ACGACACCCG GCTCTGTCAC GATGAACTGC GGCGTAAGAA AATCAGCGCG CTTATCCCTC CCCGAAAAGG TGCGGGTTAC TGGCCCGGTG AATATGCAGA 2800
CCGTAACCGT GCAGTGGCTA ATCAGCGAAT GACCGGGAGT AATGCGCGGT GGAAATGGAC AACAGATTAC AACCGTCGCT CGATAGCGGA AACGGCGATG 2900
TACCGGGTAA AACAGCTGTT CGGGGGTTCA CTGACGCTGC GTGACTACGA TGGTCAGGTT GCGGAGGCTA TGGCCCTGGT ACGAGCGCTG AACAAAATGA 3000
CGAAAGCAGG TATGCCTGAA AGCGTGCGTA TTGCCTGAAA ACACAACCCG CTACGGGGGA GACTTACCCG AAATCTGATT TATTCAACAA AGCC

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
tnpA IS903 57-980 Transposase   -
APH(3')-Ia (ARO:3002641) Tn903 1162-1977 Passenger Gene Antibiotic Resistance +
tnpA IS903 2115-3038 Transposase   +

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA IS903 924 57-980 -
Class:   Transposase
Function:   GO molecular function: DNA binding, transposase activity.
Transpoase Chemistry:   DDE
Protein Sequence:  
MAKQKFKITN WSTYNKALIN RGSLTFWLDD GAIQAWYESA TPSSRGRPQR YSDLAITTVL VIKRVFRLTL RAAQGFIDSI FTLMNVPLRC PDYSCVSRRA
KSVNISFKTF TRGEIAHLVI DSTGLKVFGE GEWKVKKHGQ ERRRIWRKLH LAVDSKTHEI ICADLSLNNV TDSEAFPGLI RQTHRKIRAA SADGAYDTRL
CHDELRRKKI SALIPPRKGA GYWPGEYADR NRAVANQRMT GSNARWKWTT DYNRRSIAET AMYRVKQLFG GSLTLRDYDG QVAEAMALVR ALNKMTKAGM
PESVRIA

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(3')-Ia (ARO:3002641) APH(3')-Ia Tn903 816 1162-1977 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(3') (ARO:3000126)
Comment:   strict match to reference sequence for ARO:3002641 (bitscore: 560)||Synonyms: aphA-1, apha1-1AB, APH(3')-Ic, apha7
Protein Sequence:  
MSHIQRETSC SRPRLNSNMD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP ELFLKHGKGS VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA
IPGKTAFQVL EEYPDSGENI VDALAVFLRR LHSIPVCNCP FNSDRVFRLA QAQSRMNNGL VDASDFDDER NGWPVEQVWK EMHKLLPFSP DSVVTHGDFS
LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS PSLQKRLFQK YGIDNPDMNK LQFHLMLDEF F

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA IS903 924 2115-3038 +
Class:   Transposase
Function:   GO molecular function: DNA binding, transposase activity.
Transpoase Chemistry:   DDE
Protein Sequence:  
MAKQKFKITN WSTYNKALIN RGSLTFWLDD GAIQAWYESA TPSSRGRPQR YSDLAITTVL VIKRVFRLTL RAAQGFIDSI FTLMNVPLRC PDYSCVSRRA
KSVNISFKTF TRGEIAHLVI DSTGLKVFGE GEWKVKKHGQ ERRRIWRKLH LAVDSKTHEI ICADLSLNNV TDSEAFPGLI RQTHRKIRAA SADGAYDTRL
CHDELRRKKI SALIPPRKGA GYWPGEYADR NRAVANQRMT GSNARWKWTT DYNRRSIAET AMYRVKQLFG GSLTLRDYDG QVAEAMALVR ALNKMTKAGM
PESVRIA

 Internal Transposable Elements (TE)     

TnCentral Accession TE Name Type Coordinates Length
IS903-V00359.1 IS903 Insertion Sequence 1-1057 1057
IS903-V00359.1 IS903 Insertion Sequence 2038-3094 1057

 Internal Repeat Elements     

Name Associated Mobile Element Coordinates Sequence (Top Strand)
IRL IS903 1040-1057 CTAAATAAGT TGTTTCGG
IRL IS903 2038-2055 GGCTTTGTTG AATAAATC

 References     

1.Oka A, Sugisaki H, Takanami M. Nucleotide sequence of the kanamycin resistance transposon Tn903. J Mol Biol. 1981 Apr 5;147(2):217-26. doi: 10.1016/0022-2836(81)90438-1. PubMed ID: 6270337