Transposon
Name: Tn5393.3
Family: Tn3        Group: Tn163
Evidence of Transposition: no
 Host     

Host Organism:Escherichia coli RPC3 Molecular Source:plasmid RCS69_pI
Place of Origin:France Date of Isolation:2018

 Map     



 Terminal Inverted Repeats (IR)     

IRL (Length: 81 bp)GGGGTCGTTTGCGGGAGAGGGCGAAATCCTACGCTAAGGCTTTGGCCAACGATATTCTCCGGTAAGATTGATGTGTTCCCA
IRR (Length: 40 bp)GGGGTCGTTTGCGGGAGGGGGCGGAATCCTACGCTAAGGC

 Sequence     
DNA SequenceLength  15282 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
GGGGTCGTTT GCGGGAGAGG GCGAAATCCT ACGCTAAGGC TTTGGCCAAC GATATTCTCC GGTAAGATTG ATGTGTTCCC AGGGGATAGG AGAAGTCGCT 100
TGATATCTAG TATGACGTCT GTCGCACCTG CTTGATCGCG GCCGCGATAG CTAGATCGCG TTGCTCCTCT TCTCCATCCG CGTTCCAAGC TGCGGAAAGG 200
CACCCATAAG CGTACGCCTG GTCGAGCAGG CGACGCGGAT CGACGTCCAG CGCACGAGAG AATGCGTCCG CCATCTGTGC AATGCGTCTA GGATCGAGAC 300
AAAGGTCGTC TCTGTCAGCC GGATCGTAGA ACATATTGGC GGCGCCAAAG CCCACTTCAC CGACCAGACC GACGGGATCT ATCACCAGCC AGCCGCGACT 400
GGAGAACATG ATGTTTTCAT GATGCAGATC GCCATGTAGC CCACGCAGTT CCGAGGCATT GCTCATCATT TGATCGGCTA TAATCGCCGC GTGGACGTAG 500
TCAGTTTGAC AACCTGCGTT TTGATCATCG CGCGCCCGCT GAAACAAAGC TGCAAAGCGA TCCCGGATCG GGAGAAGGGC AGAAGGCAGG GGTTCCTCAG 600
ATGCGGCATA CAGCTTCGCC ATTAGTTCCG CTGCAATTTC GGTCGCCTGG TAGTCGCCGT GCTCGGCAAC GATGTGAGAG AGCATTCGCT CCCCGGCATA 700
TTCGAGCAAC ATCAGATTGT TCTCACGACC GAGCAACCGG ACTGCTCCCC TCCCATTGCG CCATACCAGA TAGTCGGCCC CGCGCAGTTC ATCAGCAATG 800
TCTTCTATAG GTTTCAATCC CTTGACGATT GCAGGAGTCC CGTCTGGCAA TGAAACTTTC CAAACGAGGC TGGAAAAGGT GTCCGCAATG AGAACAGGTT 900
GCGAAACGTG CCAATGAGCA GGAAAAACAG GCGGCATGAA CATCAACCCC AAGTCAGAGG GTCCAATCGC AGATAGAAGG CAAGGCGTTC GCGGTCGGGG 1000
GCTTCGATCC CCAATACATT GAATAGGACA GCGAAGGCGC GCTCTGCTTC ATCTGGCGCT GCCCAGTTCT CTTCGGCGTT AGCAATCATG AGTGCCAAAT 1100
CGGCATAGCG ATCTGCTGTT CCGAGCCGCC CAAGGTCGAT CAGACCCGTG CATTGAAGAG TTTTAGGGTC CACCATGAAG TTCGGCATGC AGGGATCACC 1200
ATGGCAAACA ACCATATCGG TGCGCTCTTG GTCGAGCCGC ACCGGTAGCT CTCGTTCGAC ACGAGCCAAA AGATCGAGCT GCGGCGTACT CTTGTCCTCG 1300
TCCGGTAAGA AGTCGGGATT GACGGCATTG CGGGACACCA CATCAACGGC GCGTCCGAAC ATTCGCGACA GCCTGCGCTC AAACGGACAT TGATCAACCG 1400
ATAGGCTGTG AACAGCGCCA AGTTGCTGCC CCATTGACGG CCACGCTTTG AGCAAATCCG CTCCAGACAG ATCAGCCGCC GGTACTCCCG GAATTGCCGT 1500
TATCACCAAG CATGCACCCT CCTGTTCCTC CTGCCAGTTG ATCACCTCGG GGCAAGCCAC ACCTCGACCT TTGAGCCAAA TGAGGCGGTC ACGCTCTCCA 1600
GCGAGCTCAC CGCGGCGGGA AGCAGGTGCG ATTTTCGCGA AGGCATGCCC GTCACCACGT CGAAAAACAA AATCACCAGA TTCTCCGCCT CTGACAGGCA 1700
ACCAGTCAGA ATGCGATTCA CCAAAAAAAA TATTAGTTCG ATTCAATGGA GGTTCCTTCA GTTTTCTGAT GAAGCGCGGA GGTGGCTCAA CCTGCGAAAA 1800
GAAACGAGTT GCTATGGACT TGCACCGGTT GTGTTCCGGT CTATCTCTCA TTTTAAGCGG CTTTTTTCTC AAATGCCACC GGCGATTTCC AGCCGAGTGT 1900
TGAATGTCTT CGGCGTGGAT TATAAAAGCC ATTTATATAT TCGAAGATTG CAATCTCAAT ATCTCGCCTT GTTTGCCAGT GTCTGCGCCA AATCAACTCA 2000
GCCTTTAATG ATTTAAAGAA GCTTTCTACT GCGGAGTTAT CAAAACAATT GCCTTTCCCG CTCATGGACG GCAGCAATTG ATGTTTGAGC AGTAGCTTTT 2100
GATATTCATG AGCGCAATAT TGGCTCCCAC GGTCTGTGTG TTGAATACAA CCCGGTGGTG GTTTGCGTAA AGCCAACGCC ATATTCAGTG CCCTTAATGC 2200
AAGATCCTGC TTTAATCGAT CACCTGTTGC CCAGCCAATC ACGCGACGGG AATACAGGTC AAGGATAACA GCAAGATAGA CCCATCCTTC TCTGGTCCAA 2300
ACATAAGTGA TATCGCCTGC CCATTTCTGG TTGGGTGCGC TTGCGCTAAA GTCTTGTTTT AATAGGTTCG GTGCAATGTT GAAGGTATGA TGACTATCCG 2400
TTGTCCGTTT GAATTTACGC GTTCGAACAA CTGTAATGTT ATTCTGGCGC ATCAAACGTC CAACCCGACG CTGCCCAACC TGCAGGCCCA GCGCTTTCAA 2500
CTCTTCTGTC ATACGCGGCC TACCATAGCT CCCCAAACAC AACCGATGCT GCTCACGTAT ATGCGCTAGA AGTATAAGAT CACGACGCTG GCGCAGTGAT 2600
GGAGGACGGC GTTTCCATGC ACGTAAACCA CGATCTGTTA CGCCCATCAA ACGACATATG CGTGAACGTG AGAGAGAGCC ACGGTAATCC GTAATAAACT 2700
GAAATCTCAC AGCTTTTGTA CTGCGAAAAA TATTGCTGCC TTTTTTAATA TCTCCCTCTC CTCCCGAAGG ATACGGTTCT CTTTGCGTAA ACGTTCATTC 2800
TCACGCAGAA GATCAGTGTC TTGGGTAGGA ATTTTAGTTT CATCGGAAAT TGATGCGATC CATTTCCCAA GCGTGGAAAG CCCAATACTT AAATCTGACG 2900
CAACTTGACG GCGTGTTAAG CCACTAGTGA GTGCTATGCG AACTGCATCA CGCTTAAATT CATCACTATG CTTTAATGAC ATATATGGTC TCCTTGATAA 3000
CAAATAATGC TCTCAAAAAG ACCGGAACGA AACCGAGGCA AGTCCACTAC GTAAGTCCGA GAACATGCTT TCCATGGTCT CTGAGCTCGC CTTTGGGACC 3100
GACATATCGG TAGAGAGTGA CGCGCTCGAT GCCGAGTTCC TTGCAGAGAT CGGAAACTGA AGTATCGCGC TGGGCCATGG CGGCTTGCGC GAGACGCACC 3200
TGAGCTTTGG TGAGCGCGAA TTTTCGTCCG CCCTTGCGAC CGCGCGCTCT CGCGGAGGCG AGACCCGCCA TGGTGCGCTC TCGGATCAGA TCCCGCTCGA 3300
ACTCGGCCAA GGTGGCGAAG ATTCCGAACA CCATGCGACC GGACGCAGTC GTGGTGTCGA TCTGAGCGCC CTTTCCAGTC AGAACCCGCA GGCCGATCTT 3400
GCGGTCTGAC AGCTCCTTCA CCGTGTTGAC CAGATGGGCA AGCGATCGTC CGAGGCGATC GAGCTTCCAG ACCACCAGCA CATCGCCGTC ACGCAATGAC 3500
TTGAGGCAGG CAGTCAAGCC AGGGCGATCA TCACGACCGC CGGAAGCAAG ATCATCATAG ATATTGTCCC GTTCGACACC TGCGGCGCGC AAGGCGTCGT 3600
GCTGCAGGTC GAGAGACTGC GAGCCATCGG CTTTGGAGAC GCGGGCATAT CCGATCAGCA TGTATCACAA ACGTTGGTTT GAGGCGGCGC TTCGGCCACG 3700
ATTGCATTGA CCTCTGGAAA TGTATCTCAA CCAGCTTCAT AAACAAAGCG TCTTGAACGC TATCAGATTT TGAAAAAGGA ACATGTATGC CGCGTCGCGT 3800
CACTCTAACC GATCGGCAGA AAGACGCGCT GTTGCGCTTG CCGACTTCAC AGACGGATTT GCTCAAGCAC TATACGCTGA GTGATGAAGA CCTTGGGCAT 3900
ATCAGGCTGC GTCGGCGCGC TCACAACAGG TTCGGCTTCG CCCTGCAATT GTGTGTCCTG CGCTATCCCG GCCGGGTGCT GGCTCCAGGC GAACTGATCC 4000
CTGCAGAGGT CATCGAATTT ATCGGAGCGC AGCTTGGCCT GGGTGCCGAC GATCTCGTAG ACTATGCTGC CCGCGAGGAA ACACGGCACG AGCATCTTGC 4100
CGAGTTACGG GGGCTCTACG GCTTCCGCAC CTTCTCCGGA CGTGGTGCGA GCGAGCTGAA GGAATGGTTG TTCCGAGAAG CCGAGATGGC GGTGTCGAAC 4200
GAGGATATCG CCCGTCGCTT CGTAGCCGAG TGCCGACGCA CCCGCACTGT CCTTCCCGCG ACATCCACGA TCGAGCGGCT TTGTGCCGCG GCTCTCGTCG 4300
ATGCCGAGCG ACGCATCGAG ACGAGGATCG CCAGTCGGCT GCCTATGTCG ATCCGAGAAC AGTTGCTGGC ATTGCTCGAG GAGACGGCTG ATGATCGGGT 4400
GACCCGTTTT GTGTGGCTGC GCCAGTTCGA GCCTGGCTCG AACTCTTCGT CGGCCAACCG GCTGCTCGAC CGGCTCGAAT ATCTGCAACG CATCGATCTC 4500
CCCGAGGATC TGCTTGCCGG CGTTCCTGCC CATCGGGTGA CTCGTCTGCG CAGGCAGGGT GAACGGTATT ATGCCGACGG CATGCGCGAT CTCCCGGAGG 4600
ACAGGCGGCT TGCGATCTTG GCTGTTTGCG TCTCGGAATG GCAGGCGATG TTGGCCGACG CAGTGGTCGA AACCCACGAC CGGATCGTCG GCCGTCTCTA 4700
CCGTGCTTCG GAGCGTATTT GCCATGCAAA GGTCGCAGAC GAAGCGGGGG TGGTGCGTGA CACCCTGAAA TCCTTCGCCG AGATCGGGGG CGCCCTGGTC 4800
GATGCACAGG ATGATGGCCA GCCGCTGGGC GATGTCATCG CGAGTGGGTC AGGGTGGGAC GGCTTAAAAA CCCTTGTTGC TGACTCTTAT ACACAAGTAG 4900
CGTCCTGAAC GGAACCTTTC CCGTTTTCCA GGATCTGATC TTCCATGTGA CCTCCTAACA TGGTAACGTT CATGATAACT TCTGCTCTTC ATCGTGCGGC 5000
CGACTGGGCT AAATCTGTGT TCTCTTCGGC GGCGCTGGGT GATCCTCGCC GTACTGCCCG CTTGGTTAAC GTCGCCGCCC AATTGGCAAA ATATTCTGGT 5100
AAATCAATAA CCATCTCATC AGAGGGTAGT GAAGCCATGC AGGAAGGCGC TTACCGATTT ATCCGCAATC CCAACGTTTC TGCCGAGGCG ATCAGAAAGG 5200
CTGGCGCCAT GCAAACAGTC AAGTTGGCTC AGGAGTTTCC CGAACTGCTG GCCATTGAGG ACACCACCTC TTTGAGTTAT CGCCACCAGG TCGCCGAAGA 5300
GCTTGGCAAG CTGGGCTCTA TTCAGGATAA ATCCCGCGGA TGGTGGGTTC ACTCCGTTCT CTTGCTCGAG GCCACCACAT TCCGCACCGT AGGATTACTG 5400
CATCAGGAGT GGTGGATGCG CCCGGATGAC CCTGCCGATG CGGATGAAAA GGAGAGTGGC AAATGGCTGG CAGCGGCCGC AACTAGCCGG TTACGCATGG 5500
GCAGCATGAT GAGCAACGTG ATTGCGGTCT GTGACCGCGA AGCCGATATT CATGCTTATC TGCAGGACAA ACTGGCGCAT AACGAGCGCT TCGTGGTGCG 5600
CTCCAAGCAC CCACGCAAGG ACGTAGAGTC TGGGTTGTAT CTGTACGACC ATCTGAAGAA CCAACCGGAG TTGGGTGGCT ATCAGATCAG CATTCCGCAA 5700
AAGGGCGTGG TGGATAAACG CGGTAAACGT AAAAATCGAC CAGCCCGCAA GGCGAGCTTG AGCCTGCGCA GTGGGCGCAT CACGCTAAAA CAGGGGAATA 5800
TCACGCTCAA CGCGGTGCTG GCCGAGGAGA TTAACCCGCC CAAGGGTGAG ACCCCGTTGA AATGGTTGTT GCTGACCAGC GAACCGGTCG AGTCGCTAGC 5900
CCAAGCCTTG CGCGTCATCG ACATTTATAC CCATCGCTGG CGGATCGAGG AGTTCCATAA GGCATGGAAA ACCGGAGCAG GAGCCGAGAG GCAACGCATG 6000
GAGGAGCCGG ATAATCTGGA GCGGATGGTC TCGATCCTCT CGTTTGTTGC GGTCAGGCTG TTACAGCTCA GAGAAAGCTT CACGCTGCCG CAAGCACTCA 6100
GGGCGCAAGG GCTGCTAAAG GAAGCGGAAC ACGTAGAAAG CCAGTCCGCA GAAACGGTGC TGACCCCGGA TGAATGTCAG CTACTGGGCT ATCTGGACAA 6200
GGGAAAACGC AAGCGCAAAG AGAAAGCAGG TAGCTTGCAG TGGGCTTACA TGGCGATAGC TAGACTGGGC GGTTTTATGG ACAGCAAGCG AACCGGAATT 6300
GCCAGCTGGG GCGCCCTCTG GGAAGGTTGG GAAGCCCTGC AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT GGCGCAGGGG ATCAAGATCT 6400
GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT 6500
GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 6600
GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 6700
TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC 6800
ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 6900
TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 7000
GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG 7100
CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 7200
TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 7300
CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCGG 7400
GCTCGATCCC CTCGCGAGTT GGTTCAGCTG CTGCCTGAGG CTGGACGACC TCGCGGAGTT CTACCGGCAG TGCAAATCCG TCGGCATCCA GGAAACCAGC 7500
AGCGGCTATC CGCGCATCCA TGCCCCCGAA CTGCAGGAGT GGGGAGGCAC GATGGCCGCT TTGGTCGACC CGGACGGGAC GCTCCTGCGC CTGATACAGA 7600
ACGAATTGCT TGCAGGCATC TCATGAGTGT GTCTTCCCGT TTTCCGCCTG AGGTCACTGC GTGGATGGAG CGCTGGCGCC TGCTGCGCGA CGGCGAGCTG 7700
CTCACCACCC ACTCGAGCTG GATACTTCCC GTCCGCCAGG GGGACATGCC GGCGATGCTG AAGGTCGCGC GCATTCCCGA TGAAGAGGCC GGTTACCGCC 7800
TGTTGACCTG GTGGGACGGG CAGGGCGCCG CCCGAGTCTT CGCCTCGGCG GCGGGCGCTC TGCTCATGGA GCGCGCGTCC GGGGCCGGGG ACCTTGCACA 7900
GATAGCGTGG TCCGGCCAGG ACGACGAGGC TTGCAGGATC CTCTGCGACA CCGCCGCTCG TCTGCACGCG CCGCGGTCCG GACCGCCGCC CGATCTCCAT 8000
CCGCTACAGG AATGGTTCCA GCCGCTTTTC CGGTTGGCCG CTGAGCACGC GGCACTTGCG CCCGCCGCCA GCGTAGCGCG CCAACTTCTG GCGGCGCCGC 8100
GCGAGGTGTG CCCGCTCCAC GGCGACCTGC ACCACGAGAA CGTGCTCGAC TTCGGCGACC GCGGCTGGCT GGCCATCGAC CCGCACGGAC TGCTCGGCGA 8200
GCGCACCTTC GACTATGCCA ACATCTTCAC GAATCCCGAT CTCAGCGACC CCGGTCGCCC GCTTGCGATC CTGCCGGGCA GGCTGGAGGC TCGACTCAGC 8300
ATTGTGGTCG CGACGACCGG GTTTGAGCCC GAACGGCTTC TTCGCTGGAT CATTGCATGG ACGGGCTTGT CGGCAGCCTG GTTCATCGGC GACGGCGACG 8400
GCGAGGGCGA GGGCGCTGCG ATTGATCTGG CCGTAAACGC CATGGCACGC CGGTTGCTTG ACTAGCGCGG TCACCGATCT CACCTGGTCG TCGAGCTAGG 8500
TCAGGCCGTG TCGGGCGTGA TCCGCTGGAA GTCGTTGCGG GCCACACCCG CCGCCTCGAA GCCCTGCACC AGGCCGGCAT CGTGGTGTGC GTGGCCGAGG 8600
GACTATGGAA GGTGCCGGAC GATCTGCCCG AGCAGGGCCG CCGCTATGAC GCCCAGCGTC TTGGTGGCGT GACGGTGGAG CTGAAATCGC ACCTGCCCAT 8700
CGAGCGGCAG GCCCGCGTGA TCGGTGCCAC CTGGCTTGAC CAGCAGTTGA TCGACGGTGG CTCGGGCTTG GGCGACCTGG GCTTTAGCAG TGAGGCCAAG 8800
TAGGCGATAC AGCAGCGCGC GGACTTCCTG GCCGAACAGG GACTGGCCGA GCGGCGCGGG CAGCGCGTGA TCCTCACCGG AATCTGCTGG GCAGCAGCGG 8900
GCTCGGGAAC TGGCGCAGGC CGCGAAGGAC ATTGCCGCCG ATACCGGCCT GGAGCATCGC CCCGTGGCCG ACGGCCAGCG CGTTGCCGGC GTCTACCGGC 9000
GCCCCGTCAT GCTCGCCAGC GGGCGAAATG GGATGCTTGA TGACGCCAAG GGGTCCAGCC TCGTGCGGTG GAAGCCCATC GAACAGCGGC TTGGGGAGCA 9100
GCTCGCCGCG ACGGTGCGCG GTGGCGGCGT GTCTTGGGAG ATTGGACGAC AGCGTGGGCC GGCGCCGGCC CACGCTGTCG TCCAATCTCC CAAGACACGC 9200
CGCCACCGCG CACCGTCGCG GCGAGCTGCT CCCCAAGCCG CTGTTCGATG GGCTTCCACC GCACGAGGCT GGACCCCTTG GCGTCATCAA GCATCCCATT 9300
TCGCCCGCTG GCGAGCATGA CGGGGCGCCG GTAGACGCCG GCAACGCGCT GGCCGTCGGC CACGGGGCGA TGCTCCAGGC CGGTATCGGC GGCAATGTCC 9400
TTCGCGGCCT GCGCCAGTTC CCGAGCCCGC TGCTGCCCAG CAGATTCCGG TGAGGATCAC GCGCTGCCCG CGCCGCTCGG CCAGTCCCTG TTCGGCCAGG 9500
AAGTCCGCGC GCTGCTGTAT CGCCTACTTG GCCTCACTGC TAAAGCCCAG GTCGCCCAAG CCCGAGCCAC CGTCGATCAA CTGCTGGTCA AGCCAGGTGG 9600
CACCGATCAC GCGGGCCTGC CGCTCGATGG GCAGGTGCGA TTTCAGCTCC ACCGTCACGC CACCAAGACG CTGGGCGTCA TAGCGGCGGC CCTGCTCGGG 9700
CAGATCGTCC GGCACCTTCC ATAGTCCCTC GGCCACGCAC ACCACGATGC CGGCCTGGTG CAGGGCTTCG AGGCGGCGGG TGTGGCCCGC AACGACTTCC 9800
AGCGGATCAC GCCCGACACG GCCTGACCTA GCTCGACGAC CAGGTGAGAT CGGTGACCGC GCTAGTCAAG CAACCGGCGT GCCATGGCGT TTACGGCCAG 9900
ATCAATCGCA GCGCCCTCGC CCTCGCCGTC GCCGTCGCCG ATGAACCAGG CTGCCGACAA GCCCGTCCAT GCAATGATCC AGCGAAGAAG CCGTTCGGGC 10000
TCAAACCCGG TCGTCGCGAC CACAATGCTG AGTCGAGCCT CCAGCCTGCC CGGCAGGATC GCAAGCGGGC GACCGGGGTC GCTGAGATCG GGATTCGTGA 10100
AGATGTTGGC ATAGTCGAAG GTGCGCTCGC CGAGCAGTCC GTGCGGGTCG ATGGCCAGCC AGCCGCGGTC GCCGAAGTCG AGCACGTTCT CGTGGTGCAG 10200
GTCGCCGTGG AGCGGGCACA CCTCGCGCGG CGCCGCCAGA AGTTGGCGCG CTACGCTGGC GGCGGGCGCA AGTGCCGCGT GCTCAGCGGC CAACCGGAAA 10300
AGCGGCTGGA ACCATTCCTG TAGCGGATGG AGATCGGGCG GCGGTCCGGA CCGCGGCGCG TGCAGACGAG CGGCGGTGTC GCAGAGGATC CTGCAAGCCT 10400
CGTCGTCCTG GCCGGACCAC GCTATCTGTG CAAGGTCCCC GGCCCCGGAC GCGCGCTCCA TGAGCAGAGC GCCCGCCGCC GAGGCGAAGA CTCGGGCGGC 10500
GCCCTGCCCG TCCCACCAGG TCAACAGGCG GTAACCGGCC TCTTCATCGG GAATGCGCGC GACCTTCAGC ATCGCCGGCA TGTCCCCCTG GCGGACGGGA 10600
AGTATCCAGC TCGAGTGGGT GGTGAGCAGC TCGCCGTCGC GCAGCAGGCG CCAGCGCTCC ATCCACGCAG TGACCTCAGG CGGAAAACGG GAAGACACAC 10700
TCATGAGATG CCTGCAAGCA ATTCGTTCTG TATCAGGCGC AGGAGCGTCC CGTCCGGGTC GACCAAAGCG GCCATCGTGC CTCCCCACTC CTGCAGTTCG 10800
GGGGCATGGA TGCGCGGATA GCCGCTGCTG GTTTCCTGGA TGCCGACGGA TTTGCACTGC CGGTAGAACT CCGCGAGGTC GTCCAGCCTC AGGCAGCAGC 10900
TGAACCAACT CGCGAGGGGA TCGAGCCCGG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 11000
TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG 11100
CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC 11200
AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGGT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 11300
TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCGCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG 11400
CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA 11500
TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 11600
CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC 11700
GCGCTGCCTC GTCCTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC 11800
AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 11900
AACGATCCTC ATCCTGTCTC TTGATCAGAT CTTGATCCCC TGCGCCATCA GATCCTTGGC GGCAAGAAAG CCATCCAGTT TACTTTGCAG GGCTTCCCAA 12000
CCTTCCCAGA GGGCGCCCCA GCTGGCAATT CCGGTTCGCT TGCTGTCCAT AAAACCGCCC AGTCTAGCTA TCGCCATGTA AGCCCACTGC AAGCTACCTG 12100
CTTTCTCTTT GCGCTTGCGT TTTCCCTTGT CCAGATAGCC CAGTAGCTGA CATTCATCCG GGGTCAGCAC CGTTTCTGCG GACTGGCTTT CTACGTGTTC 12200
CGCTTCCTTT AGCAGCCCTT GCGCCCTGAG TGCTTGCGGC AGCGTGAAGC TTTCTCTGAG CTGTAACAGC CTGACCGCAA CAAACGAGAG GATCGAGACC 12300
ATCCGCTCCA GATTATCCGG CTCCTCCATG CGTTGCCTCT CGGCTCCTGC TCCGGTTTTC CATGCCTTAT GGAACTCCTC GATCCGCCAG CGATGGGTAT 12400
AAATGTCGAT GACGCGCAAG GCTTGGGCTA GCGACTCGAC CGGTTCGCTG GTCAGCAACA ACCATTTCAA CGGGGTCTCA CCCTTGGGCG GGTTAATCTC 12500
CTCGGCCAGC ACCGCGTTGA GCGTGATATT CCCCTGTTTT AGCGTGATGC GCCCACTGCG CAGGCTCAAG CTCGCCTTGC GGGCTGGTCG ATTTTTACGT 12600
TTACCGCGTT TATCCACCAC GCCCTTTTGC GGAATGCTGA TCTGATAGCC ACCCAACTCC GGTTGGTTCT TCAGATGGTC GTACAGATAC AACCCAGACT 12700
CTACGTCCTT GCGTGGGTGC TTGGAGCGCA CCACGAAGCG CTCGTTATGC GCCAGTTTGT CCTGCAGATA AGCATGAATA TCGGCTTCGC GGTCACAGAC 12800
CGCAATCACG TTGCTCATCA TGCTGCCCAT GCGTAACCGG CTAGTTGCGG CCGCTGCCAG CCATTTGCCA CTCTCCTTTT CATCCGCATC GGCAGGGTCA 12900
TCCGGGCGCA TCCACCACTC CTGATGCAGT AATCCTACGG TGCGGAATGT GGTGGCCTCG AGCAAGAGAA CGGAGTGAAC CCACCATCCG CGGGATTTAT 13000
CCTGAATAGA GCCCAGCTTG CCAAGCTCTT CGGCGACCTG GTGGCGATAA CTCAAAGAGG TGGTGTCCTC AATGGCCAGC AGTTCGGGAA ACTCCTGAGC 13100
CAACTTGACT GTTTGCATGG CGCCAGCCTT TCTGATCGCC TCGGCAGAAA CGTTGGGATT GCGGATAAAT CGGTAAGCGC CTTCCTGCAT GGCTTCACTA 13200
CCCTCTGATG AGATGGTTAT TGATTTACCA GAATATTTTG CCAATTGGGC GGCGACGTTA ACCAAGCGGG CAGTACGGCG AGGATCACCC AGCGCCGCCG 13300
AAGAGAACAC AGATTTAGCC CAGTCGGCCG CACGATGAAG AGCAGAAGTT ATCATGAACG TTACCATGTT AGGAGGTCAC ATGGAAGATC AGATCCTGGA 13400
AAACGGGAAA GGTTCCGTTC AGGACGCTAC TTGTGTATAA GAGTCAGCCC TTGTTGCAAT GGCAACCAGG CTGACCGCCA CCATGGCCGA CGATCCGCTC 13500
AATCATGTGC TCGACGGTTA TCACCGCTTC CGCCGATACG CTCCACGCAT GTTGCGCCTG CTCGATCTGC GAGCTGCGCC CGTTGCACTG CCGCTTCTGG 13600
AAGCGGTGAC GGCCCTTCGT ACCGGTTTGA ACGATGCCGC GATGACCAGC TTCTTGCGGC CCAGCTCGAA ATGGCATCGC CACCTTCGGG CCCAGAGGGC 13700
TGGCGACGCT CGCCTATGGG AGATCGCGGT GCTGTTCCAT CTGCGCGATG CGTTCCGCTC CGGAGATGTC TGGCTTACTA GGTCCCGGCG CTATGGCGAT 13800
CTGAAACACG CACTCGTTCC GGCACAATCC ATCGCGGAAG GCGGTCGTCT CGCTGTGCCA TTGCGGCCGG AGGAATGGCT GGCAGACCGG CAAGCTCGCC 13900
TCGACATGCG GTTGCGCGAG CTTGGCCGTG CCGCTCGCGC AGGCACGATC CCGGGCGGGT CGATTGAAAA CGGCGTTCTG CATATCGAGA AACTCGAAGC 14000
CGCCGCGCCG ACAGGCGCCG AAGATCTGGT GCTCGATCTC TACAAGCAGA TCCCGCCCAC GCGCATCACC GATCTCCTGC TGGAGGTGGA TGCGGCGACC 14100
GGCTTCACCG AAGCGTTCAC CCATCTGCGC ACAGGAGCAC CCTGCGCTGA CCGGATCGGG CTAATGAACG TTATCTTGGC GGAAGGGATC AACCTCGGCT 14200
TGCGCAAAAT GGCGGATGCG ACAAACACCC ACACCTTCTG GGAATTGATC CGCATTGGAC GGTGGCATGT CGAGGGCGAA GCCTATGACC GGGCGCTGGC 14300
CATGGTGGTC GAGGCACAGG CAGCGTTACC CATGGCCCGG TTCTGGGGCA TGGGCACGTC GGCTTCGAGC GACGGACAGT TCTTCGTCGC TACAGAGCAA 14400
GGTGAGGCCA TGAACCTGGT CAACGCGAAA TATGGCAATA CCCCGGGCCT GAAAGCCTAT AGCCACGTCT CCGACCAATA TGCGCCGTTC GCAACCCAGG 14500
TGATTCCTGC AACGGCAAGC GAAGCGGCTT ACATCCTCGA TGGCCTGCTG ATGAACGATG CTGGACGCCA TATCCGCGAG CAGTTCACCG ACACGGGCGG 14600
CTTCACCGAT CACGTCTTTG CCGCATGTGC CATTCTCGGC TACCGGTTCG CTCCGCGCAT CCGCGACCTG CCATCCAAAC GGCTCTACGC GTTCAATCCG 14700
TCGGCCGCCC CGGCGCACCT GCGAGCGTTG ATCGGCGGAA AGGTCAACCA AGCCATGATC GAGCGCAATT GGCCCGACAT CCTGCGCATC GCCGCCACCA 14800
TTGCTGCCGG GACCGTCGCG CCAAGCCAGA TTCTGCGGAA ACTCGCCTCC TATCCGCGGC AGAACGAGCT CGCGACAGCC CTGCGGGAAG TCGGTCGCGT 14900
CGAGCGCACC CTGTTCATGA TCGACTGGAT TCTGGATGCC GAACTCCAAC GGCGTGCCCA GATCGGGCTC AACAAAGGCG AAGCTCATCA TGCGCTGAAG 15000
CGGGCAATCA GCTTCCACCG CCGCGGTGAA ATCCGCGACC GTTCCGCCGA AGGCCAGCAT TACCGCATCG CCGGCATGAA TCTGCTCGCC GCCATCATCA 15100
TCTTCTGGAA CACCATGAAG CTCGGCGAGG TCGTTGCAAA CCAGAAACGC GATGGAAAGC TGCTATCGCC CGATCTCTTG GCCCATGTTT CGCCGCTCGG 15200
ATGGGAACAC ATCAATCTCA CCGGAGAATA TCGCTGGCCA AAGCCTTAGC GTAGGATTCC GCCCCCTCCC GCAAACGACC CC

 Recombination Sites     

Name Coordinates Gene Sequence
res 3565-3786 222 TGTCCCGTTC GACACCTGCG GCGCGCAAGG CGTCGTGCTG CAGGTCGAGA GACTGCGAGC
CATCGGCTTT GGAGACGCGG GCATATCCGA TCAGCATGTA TCACAAACGT TGGTTTGAGG
CGGCGCTTCG GCCACGATTG CATTGACCTC TGGAAATGTA TCTCAACCAG CTTCATAAAC
AAAGCGTCTT GAACGCTATC AGATTTTGAA AAAGGAACAT GT

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
APH(6)-Id (ARO:3002660) Tn5393.3 107-952 Passenger Gene Antibiotic Resistance -
APH(3'')-Ib (ARO:3002639) Tn5393c 943-1746 Passenger Gene Antibiotic Resistance -
orfAB IS1133 1853-2982 Transposase   -
orfB IS1133 1853-2710 Transposase   -
orfA IS1133 2707-2982 Accessory Gene Regulator -
tnpR Tn5393.3 3050-3661 Accessory Gene Resolvase -
tnpA 5'-end Tn5393.3 3787-4908 Transposase   +
tnp IS50R 4972-6402 Transposase   +
inh IS50R 5137-6401 Accessory Gene Inhibitor +
APH(3')-IIa (ARO:3002644) Tn5.1 6431-7225 Passenger Gene Antibiotic Resistance +
BRP(MBL) (ARO:3001205) Tn5.1 7246-7626 Passenger Gene Antibiotic Resistance +
APH(6)-Ic (ARO:3002659) Tn5.1 7665-8465 Passenger Gene Antibiotic Resistance +
APH(6)-Ic (ARO:3002659) Tn5.1 9862-10662 Passenger Gene Antibiotic Resistance -
BRP(MBL) (ARO:3001205) Tn5.1 10701-11081 Passenger Gene Antibiotic Resistance -
APH(3')-IIa (ARO:3002644) Tn5.1 11102-11896 Passenger Gene Antibiotic Resistance -
tnp IS50R 11925-13355 Transposase   -
inh IS50R 11926-13190 Accessory Gene Inhibitor -
tnpA 3'-end Tn5393.3 13459-15249 Transposase   +

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(6)-Id (ARO:3002660) APH(6)-Id Tn5393.3 846 107-952 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(6) (ARO:3000151)
Comment:   strB, orfI || strict match to reference sequence for ARO:3002660 (bitscore: 568)
Protein Sequence:  
MGLMFMPPVF PAHWHVSQPV LIADTFSSLV WKVSLPDGTP AIVKGLKPIE DIADELRGAD YLVWRNGRGA VRLLGRENNL MLLEYAGERM LSHIVAEHGD
YQATEIAAEL MAKLYAASEE PLPSALLPIR DRFAALFQRA RDDQNAGCQT DYVHAAIIAD QMMSNASELR GLHGDLHHEN IMFSSRGWLV IDPVGLVGEV
GFGAANMFYD PADRDDLCLD PRRIAQMADA FSRALDVDPR RLLDQAYAYG CLSAAWNADG EEEQRDLAIA AAIKQVRQTS Y

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(3'')-Ib (ARO:3002639) APH(3'')-Ib Tn5393c 804 943-1746 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(3'') (ARO:3000127)
Comment:   strA, orfH || perfect match to reference sequence for ARO: 3002639
Protein Sequence:  
MNRTNIFFGE SHSDWLPVRG GESGDFVFRR GDGHAFAKIA PASRRGELAG ERDRLIWLKG RGVACPEVIN WQEEQEGACL VITAIPGVPA ADLSGADLLK
AWPSMGQQLG AVHSLSVDQC PFERRLSRMF GRAVDVVSRN AVNPDFLPDE DKSTPQLDLL ARVERELPVR LDQERTDMVV CHGDPCMPNF MVDPKTLQCT
GLIDLGRLGT ADRYADLALM IANAEENWAA PDEAERAFAV LFNVLGIEAP DRERLAFYLR LDPLTWG

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
orfAB OrfAB IS1133 1130 1853-2982 -
Class:   Transposase
Transpoase Chemistry:   DDE
Comment:   fusion protein from -1 programmed frameshifting between orfA and orfB
Protein Sequence:  
MSLKHSDEFK RDAVRIALTS GLTRRQVASD LSIGLSTLGK WIASISDETK IPTQDTDLLR ENERLRKENR ILREEREILK KAAIFFAVQK L*DFSLLRIT
VALSHVHAYV V*WA*QIVVY VHGNAVLHHC ASVVILYF*R IYVSSIGCVW GAMVGRV*QK S*KRWACRLG SVGLDV*CAR ITLQLFERVN SNGQRIVIIP
STLHRTY*NK TLAQAHPTRN GQAISLMFGP EKDGSILLLS LTCIPVA*LA GQQVID*SRI LH*GH*IWRW LYANHHRVVF NTQTVGANIA LMNIKSYCSN
INCCRP*AGK AIVLITPQ*K ASLNH*RLS* FGADTGKQGE ILRLQSSNI* MAFIIHAEDI QHSAGNRRWH LRKKPL

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
orfB OrfB IS1133 858 1853-2710 -
Class:   Transposase
Function:   transposase activity (GO:0004803)
Transpoase Chemistry:   DDE
Comment:   orfB, together with orfA, encodes a fusion protein formed by translational frame-shifting that is the transposase for IS1133
Protein Sequence:  
MRFQFITDYR GSLSRSRICR LMGVTDRGLR AWKRRPPSLR QRRDLILLAH IREQHRLCLG SYGRPRMTEE LKALGLQVGQ RRVGRLMRQN NITVVRTRKF
KRTTDSHHTF NIAPNLLKQD FSASAPNQKW AGDITYVWTR EGWVYLAVIL DLYSRRVIGW ATGDRLKQDL ALRALNMALA LRKPPPGCIQ HTDRGSQYCA
HEYQKLLLKH QLLPSMSGKG NCFDNSAVES FFKSLKAELI WRRHWQTRRD IEIAIFEYIN GFYNPRRRHS TLGWKSPVAF EKKAA

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
orfA OrfA IS1133 276 2707-2982 -
Class:   Accessory Gene
Sub Class:   Regulator
Function:   transposase activity (GO:0004803)
Comment:   orfA, together with orfB, encodes a fusion protein formed by translational frame-shifting that is the transposase for IS1133
Protein Sequence:  
MSLKHSDEFK RDAVRIALTS GLTRRQVASD LSIGLSTLGK WIASISDETK IPTQDTDLLR ENERLRKENR ILREEREILK KAAIFFAVQK L

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpR TnpR Tn5393.3 612 3050-3661 -
Class:   Accessory Gene
Sub Class:   Resolvase
Function:   recombinaseactivity (GO:0000150)
Transpoase Chemistry:   Serine
Sequence Family:  Serine Site-Specific Recombinase
Protein Sequence:  
MLIGYARVSK ADGSQSLDLQ HDALRAAGVE RDNIYDDLAS GGRDDRPGLT ACLKSLRDGD VLVVWKLDRL GRSLAHLVNT VKELSDRKIG LRVLTGKGAQ
IDTTTASGRM VFGIFATLAE FERDLIRERT MAGLASARAR GRKGGRKFAL TKAQVRLAQA AMAQRDTSVS DLCKELGIER VTLYRYVGPK GELRDHGKHV
LGLT

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA 5'-end N Tn5393.3 1122 3787-4908 +
Class:   Transposase
Transpoase Chemistry:   DDE
Comment:   disruped by insertion of Tn5.1
Protein Sequence:  
MPRRVTLTDR QKDALLRLPT SQTDLLKHYT LSDEDLGHIR LRRRAHNRFG FALQLCVLRY PGRVLAPGEL IPAEVIEFIG AQLGLGADDL VDYAAREETR
HEHLAELRGL YGFRTFSGRG ASELKEWLFR EAEMAVSNED IARRFVAECR RTRTVLPATS TIERLCAAAL VDAERRIETR IASRLPMSIR EQLLALLEET
ADDRVTRFVW LRQFEPGSNS SSANRLLDRL EYLQRIDLPE DLLAGVPAHR VTRLRRQGER YYADGMRDLP EDRRLAILAV CVSEWQAMLA DAVVETHDRI
VGRLYRASER ICHAKVADEA GVVRDTLKSF AEIGGALVDA QDDGQPLGDV IASGSGWDGL KTLVADSYTQ VAS

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp Tnp IS50R 1431 4972-6402 +
Class:   Transposase
Transpoase Chemistry:   DDE
Comment:   99% identical to tnp (Tn5)
Protein Sequence:  
MITSALHRAA DWAKSVFSSA ALGDPRRTAR LVNVAAQLAK YSGKSITISS EGSEAMQEGA YRFIRNPNVS AEAIRKAGAM QTVKLAQEFP ELLAIEDTTS
LSYRHQVAEE LGKLGSIQDK SRGWWVHSVL LLEATTFRTV GLLHQEWWMR PDDPADADEK ESGKWLAAAA TSRLRMGSMM SNVIAVCDRE ADIHAYLQDK
LAHNERFVVR SKHPRKDVES GLYLYDHLKN QPELGGYQIS IPQKGVVDKR GKRKNRPARK ASLSLRSGRI TLKQGNITLN AVLAEEINPP KGETPLKWLL
LTSEPVESLA QALRVIDIYT HRWRIEEFHK AWKTGAGAER QRMEEPDNLE RMVSILSFVA VRLLQLRESF TLPQALRAQG LLKEAEHVES QSAETVLTPD
ECQLLGYLDK GKRKRKEKAG SLQWAYMAIA RLGGFMDSKR TGIASWGALW EGWEALQSKL DGFLAAKDLM AQGIKI

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
inh Inh IS50R 1265 5137-6401 +
Class:   Accessory Gene
Sub Class:   Inhibitor
Function:   Inhibitor of transposition
Comment:   99% identical to inh (IS50R)|this is an internal start in the transposase gene from an internal promoter
Protein Sequence:  
MQEGAYRFIR NPNVSAEAIR KAGAMQTVKL AQEFPELLAI EDTTSLSYRH QVAEELGKLG SIQDKSRGWW VHSVLLLEAT TFRTVGLLHQ EWWMRPDDPA
DADEKESGKW LAAAATSRLR MGSMMSNVIA VCDREADIHA YLQDKLAHNE RFVVRSKHPR KDVESGLYLY DHLKNQPELG GYQISIPQKG VVDKRGKRKN
RPARKASLSL RSGRITLKQG NITLNAVLAE EINPPKGETP LKWLLLTSEP VESLAQALRV IDIYTHRWRI EEFHKAWKTG AGAERQRMEE PDNLERMVSI
LSFVAVRLLQ LRESFTLPQA LRAQGLLKEA EHVESQSAET VLTPDECQLL GYLDKGKRKR KEKAGSLQWA YMAIARLGGF MDSKRTGIAS WGALWEGWEA
LQSKLDGFLA AKDLMAQGIK I

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(3')-IIa (ARO:3002644) APH(3')-IIa Tn5.1 795 6431-7225 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(3') (ARO:3000126)
Protein Sequence:  
MIEQDGLHAG SPAAWVERLF GYDWAQQTIG CSDAAVFRLS AQGRPVLFVK TDLSGALNEL QDEAARLSWL ATTGVPCAAV LDVVTEAGRD WLLLGEVPGQ
DLLSSHLAPA EKVSIMADAM RRLHTLDPAT CPFDHQAKHR IERARTRMEA GLVDQDDLDE EHQGLAPAEL FARLKARMPD GEDLVVTHGD ACLPNIMVEN
GRFSGFIDCG RLGVADRYQD IALATRDIAE ELGGEWADRF LVLYGIAAPD SQRIAFYRLL DEFF

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
BRP(MBL) (ARO:3001205) BRP(MBL) Tn5.1 381 7246-7626 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   glycopeptide antibiotic (ARO:3000081)
Sequence Family:  Bleomycin resistant protein (ARO:3004256)
Comment:   loose match 57% to reference sequence for ARO:3001205 (bitscore: 142)||Synonyms: blmS, ble, BRP, blmA, BLMT
Protein Sequence:  
MTDQATPNLP SRDFDSTAAF YERLGFGIVF RDAGWMILQR GDLMLEFFAH PGLDPLASWF SCCLRLDDLA EFYRQCKSVG IQETSSGYPR IHAPELQEWG
GTMAALVDPD GTLLRLIQNE LLAGIS

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(6)-Ic (ARO:3002659) APH(6)-Ic Tn5.1 801 7665-8465 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(6) (ARO:3000151)
Comment:   perfect match to ARO:3002659 reference sequence||Synonym: str
Protein Sequence:  
MERWRLLRDG ELLTTHSSWI LPVRQGDMPA MLKVARIPDE EAGYRLLTWW DGQGAARVFA SAAGALLMER ASGAGDLAQI AWSGQDDEAC RILCDTAARL
HAPRSGPPPD LHPLQEWFQP LFRLAAEHAA LAPAASVARQ LLAAPREVCP LHGDLHHENV LDFGDRGWLA IDPHGLLGER TFDYANIFTN PDLSDPGRPL
AILPGRLEAR LSIVVATTGF EPERLLRWII AWTGLSAAWF IGDGDGEGEG AAIDLAVNAM ARRLLD

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(6)-Ic (ARO:3002659) APH(6)-Ic Tn5.1 801 9862-10662 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(6) (ARO:3000151)
Comment:   perfect match to ARO:3002659 reference sequence||Synonym: str
Protein Sequence:  
MERWRLLRDG ELLTTHSSWI LPVRQGDMPA MLKVARIPDE EAGYRLLTWW DGQGAARVFA SAAGALLMER ASGAGDLAQI AWSGQDDEAC RILCDTAARL
HAPRSGPPPD LHPLQEWFQP LFRLAAEHAA LAPAASVARQ LLAAPREVCP LHGDLHHENV LDFGDRGWLA IDPHGLLGER TFDYANIFTN PDLSDPGRPL
AILPGRLEAR LSIVVATTGF EPERLLRWII AWTGLSAAWF IGDGDGEGEG AAIDLAVNAM ARRLLD

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
BRP(MBL) (ARO:3001205) BRP(MBL) Tn5.1 381 10701-11081 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   glycopeptide antibiotic (ARO:3000081)
Sequence Family:  Bleomycin resistant protein (ARO:3004256)
Comment:   loose match 57% to reference sequence for ARO:3001205 (bitscore: 142)||Synonyms: blmS, ble, BRP, blmA, BLMT
Protein Sequence:  
MTDQATPNLP SRDFDSTAAF YERLGFGIVF RDAGWMILQR GDLMLEFFAH PGLDPLASWF SCCLRLDDLA EFYRQCKSVG IQETSSGYPR IHAPELQEWG
GTMAALVDPD GTLLRLIQNE LLAGIS

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(3')-IIa (ARO:3002644) APH(3')-IIa Tn5.1 795 11102-11896 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(3') (ARO:3000126)
Protein Sequence:  
MIEQDGLHAG SPAAWVERLF GYDWAQQTIG CSDAAVFRLS AQGRPVLFVK TDLSGALNEL QDEAARLSWL ATTGVPCAAV LDVVTEAGRD WLLLGEVPGQ
DLLSSHLAPA EKVSIMADAM RRLHTLDPAT CPFDHQAKHR IERARTRMEA GLVDQDDLDE EHQGLAPAEL FARLKARMPD GEDLVVTHGD ACLPNIMVEN
GRFSGFIDCG RLGVADRYQD IALATRDIAE ELGGEWADRF LVLYGIAAPD SQRIAFYRLL DEFF

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp Tnp IS50R 1431 11925-13355 -
Class:   Transposase
Transpoase Chemistry:   DDE
Comment:   99% identical to tnp (Tn5)
Protein Sequence:  
MITSALHRAA DWAKSVFSSA ALGDPRRTAR LVNVAAQLAK YSGKSITISS EGSEAMQEGA YRFIRNPNVS AEAIRKAGAM QTVKLAQEFP ELLAIEDTTS
LSYRHQVAEE LGKLGSIQDK SRGWWVHSVL LLEATTFRTV GLLHQEWWMR PDDPADADEK ESGKWLAAAA TSRLRMGSMM SNVIAVCDRE ADIHAYLQDK
LAHNERFVVR SKHPRKDVES GLYLYDHLKN QPELGGYQIS IPQKGVVDKR GKRKNRPARK ASLSLRSGRI TLKQGNITLN AVLAEEINPP KGETPLKWLL
LTSEPVESLA QALRVIDIYT HRWRIEEFHK AWKTGAGAER QRMEEPDNLE RMVSILSFVA VRLLQLRESF TLPQALRAQG LLKEAEHVES QSAETVLTPD
ECQLLGYLDK GKRKRKEKAG SLQWAYMAIA RLGGFMDSKR TGIASWGALW EGWEALQSKL DGFLAAKDLM AQGIKI

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
inh Inh IS50R 1265 11926-13190 -
Class:   Accessory Gene
Sub Class:   Inhibitor
Function:   Inhibitor of transposition
Comment:   99% identical to inh (IS50R)|this is an internal start in the transposase gene from an internal promoter
Protein Sequence:  
MQEGAYRFIR NPNVSAEAIR KAGAMQTVKL AQEFPELLAI EDTTSLSYRH QVAEELGKLG SIQDKSRGWW VHSVLLLEAT TFRTVGLLHQ EWWMRPDDPA
DADEKESGKW LAAAATSRLR MGSMMSNVIA VCDREADIHA YLQDKLAHNE RFVVRSKHPR KDVESGLYLY DHLKNQPELG GYQISIPQKG VVDKRGKRKN
RPARKASLSL RSGRITLKQG NITLNAVLAE EINPPKGETP LKWLLLTSEP VESLAQALRV IDIYTHRWRI EEFHKAWKTG AGAERQRMEE PDNLERMVSI
LSFVAVRLLQ LRESFTLPQA LRAQGLLKEA EHVESQSAET VLTPDECQLL GYLDKGKRKR KEKAGSLQWA YMAIARLGGF MDSKRTGIAS WGALWEGWEA
LQSKLDGFLA AKDLMAQGIK I

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA 3'-end N Tn5393.3 1791 13459-15249 +
Class:   Transposase
Transpoase Chemistry:   DDE
Comment:   disrupted by insertion of Tn5.1
Protein Sequence:  
MATRLTATMA DDPLNHVLDG YHRFRRYAPR MLRLLDLRAA PVALPLLEAV TALRTGLNDA AMTSFLRPSS KWHRHLRAQR AGDARLWEIA VLFHLRDAFR
SGDVWLTRSR RYGDLKHALV PAQSIAEGGR LAVPLRPEEW LADRQARLDM RLRELGRAAR AGTIPGGSIE NGVLHIEKLE AAAPTGAEDL VLDLYKQIPP
TRITDLLLEV DAATGFTEAF THLRTGAPCA DRIGLMNVIL AEGINLGLRK MADATNTHTF WELIRIGRWH VEGEAYDRAL AMVVEAQAAL PMARFWGMGT
SASSDGQFFV ATEQGEAMNL VNAKYGNTPG LKAYSHVSDQ YAPFATQVIP ATASEAAYIL DGLLMNDAGR HIREQFTDTG GFTDHVFAAC AILGYRFAPR
IRDLPSKRLY AFNPSAAPAH LRALIGGKVN QAMIERNWPD ILRIAATIAA GTVAPSQILR KLASYPRQNE LATALREVGR VERTLFMIDW ILDAELQRRA
QIGLNKGEAH HALKRAISFH RRGEIRDRSA EGQHYRIAGM NLLAAIIIFW NTMKLGEVVA NQKRDGKLLS PDLLAHVSPL GWEHINLTGE YRWPKP

 Internal Transposable Elements (TE)     

TnCentral Accession TE Name Type Coordinates Length
Tn5393.3-LT985287.1 Tn5393.3 Transposon 1-15282 15282
IS1133-Z12167 IS1133 Insertion Sequence 1815-3046 1232
Tn5.1-LT985287.1 Tn5.1 Transposon 4880-13447 8568
IS50R-U00004.1 IS50R Insertion Sequence 4880-6413 1534
IS50R-U00004.1 IS50R Insertion Sequence 11914-13447 1534

 Internal Repeat Elements     

Name Associated Mobile Element Coordinates Sequence (Top Strand)
IRL Tn5393.3 1-40 GGGGTCGTTT GCGGGAGAGG GCGAAATCCT ACGCTAAGGC
IRR IS1133 1815-1841 TGGACTTGCA CCGGTTGTGT TCCGGTC
IRL IS1133 3020-3046 CTGGCCTTGC TTTGGCTCCG TTCAGGT
IRL IS50R 4880-4888 CTGACTCTT
IRR IS50R 6405-6413 TTCTCTGTC
IRR IS50R 11914-11922 CTGTCTCTT
IRL IS50R 13439-13447 TTCTCAGTC
IRRl Tn5393.3 15202-15282 ACCCTTGTGT AGTTAGAGTG GCCTCTTATA GCGACCGGTT TCGGAATCGC
ATCCTAAGGC GGGGGAGGGC GTTTGCTGGG G

 References     

1.Siguier P, Varani A, Perochon J, Chandler M. Exploring bacterial insertion sequences with ISfinder: objectives, uses, and future developments. Methods Mol Biol. 2012;859:91-103. doi: 10.1007/978-1-61779-603-6_5. PubMed ID: 22367867
2.Blot M, Hauer B, Monnet G. The Tn5 bleomycin resistance gene confers improved survival and growth advantage on Escherichia coli. Mol Gen Genet. 1994 Mar;242(5):595-601. doi: 10.1007/BF00285283. PubMed ID: 7510018
3.Yuasa K, Sugiyama M. Bleomycin-induced beta-lactamase overexpression in Escherichia coli carrying a bleomycin-resistance gene from Streptomyces verticillus and its application to screen bleomycin analogues. FEMS Microbiol Lett. 1995 Oct 1;132(1-2):61-6. doi: 10.1111/j.1574-6968.1995.tb07811.x. PubMed ID: 7590166
4.Reznikoff WS. The Tn5 transposon. Annu Rev Microbiol. 1993;47:945-63. doi: 10.1146/annurev.mi.47.100193.004501. PubMed ID: 7504907