Transposon
Name: Tn5
Family: Compound Transposon        Group: IS50
Evidence of Transposition: yes
 Host     

Host Organism:Pseudomonas aeruginosa Molecular Source:plasmid JR67
Date of Isolation:1975
Other Geographic Information:clinical isolate from Schering-Plough/Upjohn

 Map     



 Terminal Inverted Repeats (IR)     

IRL (Length: 9 bp)CTGACTCTT
IRL (Length: 9 bp)CTGACTCTT

 Sequence     
DNA SequenceLength  5818 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
CTGACTCTTA TACACAAGTA GCGTCCTGAA CGGAACCTTT CCCGTTTTCC AGGATCTGAC TTCCATGTGA CCTCCTAACA TGGTAACGTT CATGATAACT 100
TCTGCTCTTC ATCGTGCGGC CGACTGGGCT AAATCTGTGT TCTCTTCGGC GGCGCTGGGT GATCCTCGCC GTACTGCCCG CTTGGTTAAC GTCGCCGCCC 200
AATTGGCAAA ATATTCTGGT AAATCAATAA CCATCTCATC AGAGGGTAGT GAAGCCATGC AGGAAGGCGC TTACCGATTT TACCGCAATC CCAACGTTTC 300
TGCCGAGGCG ATCAGAAAGG CTGGCGCCAT GCAAACAGTC AAGTTGGCTC AGGAGTTTCC CGAACTGCTG GCCATTGAGG ACACCACCTC TTTGAGTTAT 400
CGCCACCAGG TCGCCGAAGA GCTTGGCAAG CTGGGCTCTA TTCAGGATAA ATCCCGCGGA TGGTGGGTTC ACTCCGTTCT CTTGCTCGAG GCCACCACAT 500
TCCGCACCGT AGGATTACTG CATCAGGAGT GGTGGATGCG CCCGGATGAC CCTGCCGATG CGGATGAAAA GGAGAGTGGC AAATGGCTGG CAGCGGCCGC 600
AACTAGCCGG TTACGCATGG GCAGCATGAT GAGCAACGTG ATTGCGGTCT GTGACCGCGA AGCCGATATT CATGCTTATC TGCAGGACAG GCTGGCGCAT 700
AACGAGCGCT TCGTGGTGCG CTCCAAGCAC CCACGCAAGG ACGTAGAGTC TGGGTTGTAT CTGATCGACC ATCTGAAGAA CCAACCGGAG TTGGGTGGCT 800
ATCAGATCAG CATTCCGCAA AAGGGCGTGG TGGATAAACG CGGTAAACGT AAAAATCGAC CAGCCCGCAA GGCGAGCTTG AGCCTGCGCA GTGGGCGCAT 900
CACGCTAAAA CAGGGGAATA TCACGCTCAA CGCGGTGCTG GCCGAGGAGA TTAACCCGCC CAAGGGTGAG ACCCCGTTGA AATGGTTGTT GCTGACCGGC 1000
GAACCGGTCG AGTCGCTAGC CCAAGCCTTG CGCGTCATCG ACATTTATAC CCATCGCTGG CGGATCGAGG AGTTCCATAA GGCATGGAAA ACCGGAGCAG 1100
GAGCCGAGAG GCAACGCATG GAGGAGCCGG ATAATCTGGA GCGGATGGTC TCGATCCTCT CGTTTGTTGC GGTCAGGCTG TTACAGCTCA GAGAAAGCTT 1200
CACGCTGCCG CAAGCACTCA GGGCGCAAGG GCTGCTAAAG GAAGCGGAAC ACGTAGAAAG CCAGTCCGCA GAAACGGTGC TGACCCCGGA TGAATGTCAG 1300
CTACTGGGCT ATCTGGACAA GGGAAAACGC AAGCGCAAAG AGAAAGCAGG TAGCTTGCAG TGGGCTTACA TGGCGATAGC TAGACTGGGC GGTTTTATGG 1400
ACAGCAAGCG AACCGGAATT GCCAGCTGGG GCGCCCTCTG GTAAGGTTGG GAAGCCCTGC AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT 1500
GGCGCAGGGG ATCAAGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 1600
GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 1700
ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG 1800
TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 1900
TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 2000
GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC 2100
TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 2200
CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 2300
TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG 2400
AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 2500
GAGTTCTTCG CCCACCCCGG GCTCGATCCC CTCGCGAGTT GGTTCAGCTG CTGCCTGAGG CTGGACGACC TCGCGGAGTT CTACCGGCAG TGCAAATCCG 2600
TCGGCATCCA GGAAACCAGC AGCGGCTATC CGCGCATCCA TGCCCCCGAA CTGCAGGAGT GGGGAGGCAC GATGGCCGCT TTGGTCGACC CGGACGGGAC 2700
GCTCCTGCGC CTGATACAGA ACGAATTGCT TGCAGGCATC TCATGAGTGT GTCTTCCCGT TTTCCGCCTG AGGTCACTGC GTGGATGGAG CGCTGGCGCC 2800
TGCTGCGCGA CGGCGAGCTG CTCACCACCC ACTCGAGCTG GATACTTCCC GTCCGCCAGG GGGACATGCC GGCGATGCTG AAGGTCGCGC GCATTCCCGA 2900
TGAAGAGGCC GGTTACCGCC TGTTGACCTG GTGGGACGGG CAGGGCGCCG CCCGAGTCTT CGCCTCGGCG GCGGGCGCTC TGCTCATGGA GCGCGCGTCC 3000
GGGGCCGGGG ACCTTGCACA GATAGCGTGG TCCGGCCAGG ACGACGAGGC TTGCAGGATC CTCTGCGACA CCGCCGCTCG TCTGCACGCG CCGCGGTCCG 3100
GACCGCCGCC CGATCTCCAT CCGCTACAGG AATGGTTCCA GCCGCTTTTC CGGTTGGCCG CTGAGCACGC GGCACTTGCG CCCGCCGCCA GCGTAGCGCG 3200
CCAACTTCTG GCGGCGCCGC GCGAGGTGTG CCCGCTCCAC GGCGACCTGC ACCACGAGAA CGTGCTCGAC TTCGGCGACC GCGGCTGGCT GGCCATCGAC 3300
CCGCACGGAC TGCTCGGCGA GCGCACCTTC GACTATGCCA ACATCTTCAC GAATCCCGAT CTCAGCGACC CCGGTCGCCC GCTTGCGATC CTGCCGGGCA 3400
GGCTGGAGGC TCGACTCAGC ATTGTGGTCG CGACGACCGG GTTTGAGCCC GAACGGCTTC TTCGCTGGAT CATTGCATGG ACGGGCTTGT CGGCAGCCTG 3500
GTTCATCGGC GACGGCGACG GCGAGGGCGA GGGCGCTGCG ATTGATCTGG CCGTAAACGC CATGGCACGC CGGTTGCTTG ACTAGCGCGG TCACCGATCT 3600
CACCTGGTCG TCGAGCTAGG TCAGGCCGTG TCGGGCGTGA TCCGCTGGAA GTCGTTGCGG GCCACACCCG CCGCCTCGAA GCCCTGCACC AGGCCGGCAT 3700
CGTGGTGTGC GTGGCCGAGG GACTATGGAA GGTGCCGGAC GATCTGCCCG AGCAGGGCCG CCGCTATGAC GCCCAGCGTC TTGGTGGCGT GACGGTGGAG 3800
CTGAAATCGC ACCTGCCCAT CGAGCGGCAG GCCCGCGTGA TCGGTGCCAC CTGGCTTGAC CAGCAGTTGA TCGACGGTGG CTCGGGCTTG GGCGACCTGG 3900
GCTTTAGCAG TGAGGCCAAG TAGGCGATAC AGCAGCGCGC GGACTTCCTG GCCGAACAGG GACTGGCCGA GCGGCGCGGG CAGCGCGTGA TCCTCACCGG 4000
AATCTGCTCG GCAGCAGCGG GCTCGGGAAC TGGCGCAGGC CGCGAAGGAC ATTGCCGCCG ATACCGGCCT GGAGCATCGC CCCGTGGCCG ACGGCCAGCG 4100
CGTTGCCGGC GTCTACCGGC GCCCCGTCAT GCTCGCCAGC GGGCGAAATG GGATGCTTGA TGACGCCAAG GGGTCCAGCC TCGTGCGGTG GAAGCCCATC 4200
GAACAGCGGC TTGGGGAGCA GCTCGCCGCG ACGGTGCGCG GTGGCGGCGT GTCTTGGGAG ATTGGACGAC AGCGTGGGCC GGCCCCTGTC TCTTGATCAG 4300
ATCTTGATCC CCTGCGCCAT CAGATCCTTG GCGGCAAGAA AGCCATCCAG TTTACTTTGC AGGGCTTCCC AACCTTCCCA GAGGGCGCCC CAGCTGGCAA 4400
TTCCGGTTCG CTTGCTGTCC ATAAAACCGC CCAGTCTAGC TATCGCCATG TAAGCCCACT GCAAGCTACC TGCTTTCTCT TTGCGCTTGC GTTTTCCCTT 4500
GTCCAGATAG CCCAGTAGCT GACATTCATC CGGGGTCAGC ACCGTTTCTG CGGACTGGCT TTCTACGTGT TCCGCTTCCT TTAGCAGCCC TTGCGCCCTG 4600
AGTGCTTGCG GCAGCGTGAA GCTTTCTCTG AGCTGTAACA GCCTGACCGC AACAAACGAG AGGATCGAGA CCATCCGCTC CAGATTATCC GGCTCCTCCA 4700
TGCGTTGCCT CTCGGCTCCT GCTCCGGTTT TCCATGCCTT ATGGAACTCC TCGATCCGCC AGCGATGGGT ATAAATGTCG ATGACGCGCA AGGCTTGGGC 4800
TAGCGACTCG ACCGGTTCGC CGGTCAGCAA CAACCATTTC AACGGGGTCT CACCCTTGGG CGGGTTAATC TCCTCGGCCA GCACCGCGTT GAGCGTGATA 4900
TTCCCCTGTT TTAGCGTGAT GCGCCCACTG CGCAGGCTCA AGCTCGCCTT GCGGGCTGGT CGATTTTTAC GTTTACCGCG TTTATCCACC ACGCCCTTTT 5000
GCGGAATGCT GATCTGATAG CCACCCAACT CCGGTTGGTT CTTCAGATGG TCGATCAGAT ACAACCCAGA CTCTACGTCC TTGCGTGGGT GCTTGGAGCG 5100
CACCACGAAG CGCTCGTTAT GCGCCAGCCT GTCCTGCAGA TAAGCATGAA TATCGGCTTC GCGGTCACAG ACCGCAATCA CGTTGCTCAT CATGCTGCCC 5200
ATGCGTAACC GGCTAGTTGC GGCCGCTGCC AGCCATTTGC CACTCTCCTT TTCATCCGCA TCGGCAGGGT CATCCGGGCG CATCCACCAC TCCTGATGCA 5300
GTAATCCTAC GGTGCGGAAT GTGGTGGCCT CGAGCAAGAG AACGGAGTGA ACCCACCATC CGCGGGATTT ATCCTGAATA GAGCCCAGCT TGCCAAGCTC 5400
TTCGGCGACC TGGTGGCGAT AACTCAAAGA GGTGGTGTCC TCAATGGCCA GCAGTTCGGG AAACTCCTGA GCCAACTTGA CTGTTTGCAT GGCGCCAGCC 5500
TTTCTGATCG CCTCGGCAGA AACGTTGGGA TTGCGGTAAA ATCGGTAAGC GCCTTCCTGC ATGGCTTCAC TACCCTCTGA TGAGATGGTT ATTGATTTAC 5600
CAGAATATTT TGCCAATTGG GCGGCGACGT TAACCAAGCG GGCAGTACGG CGAGGATCAC CCAGCGCCGC CGAAGAGAAC ACAGATTTAG CCCAGTCGGC 5700
CGCACGATGA AGAGCAGAAG TTATCATGAA CGTTACCATG TTAGGAGGTC ACATGGAAGT CAGATCCTGG AAAACGGGAA AGGTTCCGTT CAGGACGCTA 5800
CTTGTGTATA AGAGTCAG

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
tnp_truncated IS50L 92-1444 Transposase   +
inh_truncated IS50L 257-1444 Accessory Gene Inhibitor +
APH(3')-IIa (ARO:3002644) Tn5 1551-2345 Passenger Gene Antibiotic Resistance +
BRP(MBL) (ARO:3001205) Tn5 2366-2746 Passenger Gene Antibiotic Resistance +
APH(6)-Ic (ARO:3002659) Tn5 2785-3585 Passenger Gene Antibiotic Resistance +
tnp IS50R 4297-5727 Transposase   -
inh IS50R 4298-5562 Accessory Gene Inhibitor -

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp_truncated Tnp_truncated IS50L 1353 92-1444 +
Class:   Transposase
Transpoase Chemistry:   DDE
Comment:   truncated to 421 amino acids by ochre mutation in C-terminal region which creates a promoter for aphA2 expression||non-functional
Protein Sequence:  
MITSALHRAA DWAKSVFSSA ALGDPRRTAR LVNVAAQLAK YSGKSITISS EGSEAMQEGA YRFYRNPNVS AEAIRKAGAM QTVKLAQEFP ELLAIEDTTS
LSYRHQVAEE LGKLGSIQDK SRGWWVHSVL LLEATTFRTV GLLHQEWWMR PDDPADADEK ESGKWLAAAA TSRLRMGSMM SNVIAVCDRE ADIHAYLQDR
LAHNERFVVR SKHPRKDVES GLYLIDHLKN QPELGGYQIS IPQKGVVDKR GKRKNRPARK ASLSLRSGRI TLKQGNITLN AVLAEEINPP KGETPLKWLL
LTGEPVESLA QALRVIDIYT HRWRIEEFHK AWKTGAGAER QRMEEPDNLE RMVSILSFVA VRLLQLRESF TLPQALRAQG LLKEAEHVES QSAETVLTPD
ECQLLGYLDK GKRKRKEKAG SLQWAYMAIA RLGGFMDSKR TGIASWGALW

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
inh_truncated Inh_truncated IS50L 1188 257-1444 +
Class:   Accessory Gene
Sub Class:   Inhibitor
Comment:   truncated by ochre mutation in C-terminal end
Protein Sequence:  
MQEGAYRFYR NPNVSAEAIR KAGAMQTVKL AQEFPELLAI EDTTSLSYRH QVAEELGKLG SIQDKSRGWW VHSVLLLEAT TFRTVGLLHQ EWWMRPDDPA
DADEKESGKW LAAAATSRLR MGSMMSNVIA VCDREADIHA YLQDRLAHNE RFVVRSKHPR KDVESGLYLI DHLKNQPELG GYQISIPQKG VVDKRGKRKN
RPARKASLSL RSGRITLKQG NITLNAVLAE EINPPKGETP LKWLLLTGEP VESLAQALRV IDIYTHRWRI EEFHKAWKTG AGAERQRMEE PDNLERMVSI
LSFVAVRLLQ LRESFTLPQA LRAQGLLKEA EHVESQSAET VLTPDECQLL GYLDKGKRKR KEKAGSLQWA YMAIARLGGF MDSKRTGIAS WGALW

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(3')-IIa (ARO:3002644) APH(3')-IIa Tn5 795 1551-2345 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(3') (ARO:3000126)
Protein Sequence:  
MIEQDGLHAG SPAAWVERLF GYDWAQQTIG CSDAAVFRLS AQGRPVLFVK TDLSGALNEL QDEAARLSWL ATTGVPCAAV LDVVTEAGRD WLLLGEVPGQ
DLLSSHLAPA EKVSIMADAM RRLHTLDPAT CPFDHQAKHR IERARTRMEA GLVDQDDLDE EHQGLAPAEL FARLKARMPD GEDLVVTHGD ACLPNIMVEN
GRFSGFIDCG RLGVADRYQD IALATRDIAE ELGGEWADRF LVLYGIAAPD SQRIAFYRLL DEFF

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
BRP(MBL) (ARO:3001205) BRP(MBL) Tn5 381 2366-2746 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   glycopeptide antibiotic (ARO:3000081)
Sequence Family:  Bleomycin resistant protein (ARO:3004256)
Comment:   loose match 57% to reference sequence for ARO:3001205 (bitscore: 142)||Synonyms: blmS, ble, BRP, blmA, BLMT
Protein Sequence:  
MTDQATPNLP SRDFDSTAAF YERLGFGIVF RDAGWMILQR GDLMLEFFAH PGLDPLASWF SCCLRLDDLA EFYRQCKSVG IQETSSGYPR IHAPELQEWG
GTMAALVDPD GTLLRLIQNE LLAGIS

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(6)-Ic (ARO:3002659) APH(6)-Ic Tn5 801 2785-3585 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(6) (ARO:3000151)
Comment:   perfect match to ARO:3002659 reference sequence||Synonym: str
Protein Sequence:  
MERWRLLRDG ELLTTHSSWI LPVRQGDMPA MLKVARIPDE EAGYRLLTWW DGQGAARVFA SAAGALLMER ASGAGDLAQI AWSGQDDEAC RILCDTAARL
HAPRSGPPPD LHPLQEWFQP LFRLAAEHAA LAPAASVARQ LLAAPREVCP LHGDLHHENV LDFGDRGWLA IDPHGLLGER TFDYANIFTN PDLSDPGRPL
AILPGRLEAR LSIVVATTGF EPERLLRWII AWTGLSAAWF IGDGDGEGEG AAIDLAVNAM ARRLLD

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp Tnp IS50R 1431 4297-5727 -
Class:   Transposase
Transpoase Chemistry:   DDE
Protein Sequence:  
MITSALHRAA DWAKSVFSSA ALGDPRRTAR LVNVAAQLAK YSGKSITISS EGSEAMQEGA YRFYRNPNVS AEAIRKAGAM QTVKLAQEFP ELLAIEDTTS
LSYRHQVAEE LGKLGSIQDK SRGWWVHSVL LLEATTFRTV GLLHQEWWMR PDDPADADEK ESGKWLAAAA TSRLRMGSMM SNVIAVCDRE ADIHAYLQDR
LAHNERFVVR SKHPRKDVES GLYLIDHLKN QPELGGYQIS IPQKGVVDKR GKRKNRPARK ASLSLRSGRI TLKQGNITLN AVLAEEINPP KGETPLKWLL
LTGEPVESLA QALRVIDIYT HRWRIEEFHK AWKTGAGAER QRMEEPDNLE RMVSILSFVA VRLLQLRESF TLPQALRAQG LLKEAEHVES QSAETVLTPD
ECQLLGYLDK GKRKRKEKAG SLQWAYMAIA RLGGFMDSKR TGIASWGALW EGWEALQSKL DGFLAAKDLM AQGIKI

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
inh Inh IS50R 1265 4298-5562 -
Class:   Accessory Gene
Sub Class:   Inhibitor
Function:   Inhibitor of transposition
Comment:   has an internal start in the transposase gene from an internal promoter
Protein Sequence:  
MQEGAYRFYR NPNVSAEAIR KAGAMQTVKL AQEFPELLAI EDTTSLSYRH QVAEELGKLG SIQDKSRGWW VHSVLLLEAT TFRTVGLLHQ EWWMRPDDPA
DADEKESGKW LAAAATSRLR MGSMMSNVIA VCDREADIHA YLQDRLAHNE RFVVRSKHPR KDVESGLYLI DHLKNQPELG GYQISIPQKG VVDKRGKRKN
RPARKASLSL RSGRITLKQG NITLNAVLAE EINPPKGETP LKWLLLTGEP VESLAQALRV IDIYTHRWRI EEFHKAWKTG AGAERQRMEE PDNLERMVSI
LSFVAVRLLQ LRESFTLPQA LRAQGLLKEA EHVESQSAET VLTPDECQLL GYLDKGKRKR KEKAGSLQWA YMAIARLGGF MDSKRTGIAS WGALWEGWEA
LQSKLDGFLA AKDLMAQGIK I

 Internal Transposable Elements (TE)     

TnCentral Accession TE Name Type Coordinates Length
IS50L-U00004.1 IS50L Insertion Sequence 1-1533 1533
IS50R-U00004.1 IS50R Insertion Sequence 4286-5818 1533

 Internal Repeat Elements     

Name Associated Mobile Element Coordinates Sequence (Top Strand)
IRR IS50L 1525-1533 TTCTCTGTC
IRR IS50R 4286-4294 CTGTCTCTT

 References     

1.Berg DE. Julian Davies and the discovery of kanamycin resistance transposon Tn5. J Antibiot (Tokyo). 2017 Apr;70(4):339-346. doi: 10.1038/ja.2016.120. Epub 2016 Oct 12. PubMed ID: 27731334
2.Berg DE, Davies J, Allet B, Rochaix JD. Transposition of R factor genes to bacteriophage lambda. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32. doi: 10.1073/pnas.72.9.3628. PubMed ID: 1059152
3.Auerswald EA, Ludwig G, Schaller H. Structural analysis of Tn5. Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:107-13. doi: 10.1101/sqb.1981.045.01.019. PubMed ID: 6271452
4.Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene. 1982 Oct;19(3):327-36. doi: 10.1016/0378-1119(82)90023-3. PubMed ID: 6295884
5.Mazodier P, Cossart P, Giraud E, Gasser F. Completion of the nucleotide sequence of the central region of Tn5 confirms the presence of three resistance genes. Nucleic Acids Res. 1985 Jan 11;13(1):195-205. doi: 10.1093/nar/13.1.195. PubMed ID: 3889831
6.Johnson RC, Yin JC, Reznikoff WS. Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat. Cell. 1982 Oct;30(3):873-82. doi: 10.1016/0092-8674(82)90292-6. PubMed ID: 6291786
7.Rothstein SJ, Jorgensen RA, Yin JC, Yong-di Z, Johnson RC, Reznikoff WS. Genetic organization of Tn5. Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:99-105. doi: 10.1101/sqb.1981.045.01.018. PubMed ID: 6271497
8.Rothstein SJ, Reznikoff WS. The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology. Cell. 1981 Jan;23(1):191-9. PubMed ID: 6260374
9.Johnson RC, Reznikoff WS. DNA sequences at the ends of transposon Tn5 required for transposition. Nature. 1983 Jul 21-27;304(5923):280-2. doi: 10.1038/304280a0. PubMed ID: 6306482