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Recombination Sites | |
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| Name: Tn4401d |
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| Family: Tn3 Group: Tn3000 |
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| Evidence of Transposition: no |
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| Host |
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| Host Organism: | Klebsiella pneumoniae KP529-1 | Molecular Source: | plasmid pKP529-1 |
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| Map |
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| Terminal Inverted Repeats (IR) |
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| IRL (Length: 40 bp) |  | GGGGTTCTAAGCGGGAATCCCAGAAAATTCCGTCATTCCG |
| IRR (Length: 40 bp) | | GGGGGGGTAAGCGGGAACCCCAGAAAATTCCGCCATTCCG |
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| Sequence |
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--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
GGGGTTCTAA GCGGGAATCC CAGAAAATTC CGTCATTCCG TATGATGAAA ATTCAGTCGA CTCCTCGCTT GACGTGACGA GCGCCAAAAG CCTGCCGCAT 100
CTGCCTGGCG CGCTCGAGCT CATCGCCATG CAAATACATC GACGTCGTGG CGATCGACGC ATGGCGTAGG TTGTCGCGCA CCGCGGTGAG CTGAGCTCCC 200
CGGGCCAACG CATGGCTGGC ATGACTGTGA CGCATCCAAT GAGGCGTGGC ACGGCGCAGC TTTTCGGCGG CGACCGGATG ATCGGCCTCA ATGGCCTCAG 300
CGGCTTGCGT AAAGAATCTT CGCAACACGC ACCAAAGGTG AGGGCCGGTG ATGCCGGCAT TACCGTCTTC CCCAAGACTG CCCACCAGGG GCGTTTTCGG 400
ATTCCACCGT TCCCGGCTGA CTGGCAGTTG ACGCTGTGCC AAGTATTGGT CCAGCGCCGT ATACGCGAGC GGCGGCAAGG CCACCTTACC TGGTTTGCCA 500
CCTTTGCCAA CCAAATGCAG CCAGTGGTCA CCGTGCTCGT CGAGGCGGAC ATTACCCAGG ACGGCGCCGA CCAGTTCGCT AGCGCGCAGG CCCGTGGCAT 600
AGCCGAAGTC GAGCATGAAG CGCAGGCGTT GTGCTGCCGG CTCGGACCAG CCGTAGGACC ATTCCAGGCC GTCTGCAATG GCGCGCAGCA GTAGCCATTC 700
TCCCTCGGTA AAGCCTCGCG CCGTATCCAG TGCGGGCCGC AAGGCGTGAC CGCGTACCTT GACGCCGGCG AAAGGGTTGG CCAGCACATA GCGCTGCTCG 800
ATGAGCCACC GGAACAGCGC CGAGAGCACC GTCAGCGCAT ACGCTGCCGA GCGTGCGGAC AAGCCGCCCG AGAAGGGGCG CCATTCCACC GAATCGCGTG 900
GTCGAGGTGG CCCCACCCAA CGCTCGCGCG GGGTCGGGTG CCGGACAAAG GCCCGGTACG CGATTGCATC GTCCGTGGTC AGGGATGATA GCGCCCGTCC 1000
GCGTTCGACG ATGGCCCACA GGATTAGCCG CGCAGCCTCC TTGCGATAGG CGCGCTGTGT GGCGACAGTC TCGTGCAGCG CCAGCCAGGC CTGCACGGCC 1100
TCGTAGTCGT TGGTCGCATT CAGCGTGCAA GCGTTGCGCG GGGCCCGGAA ATTGCCCTGC GAGCCGTCGA CTTCGTTCGG CAGACGCAAC TGCTCCCACG 1200
GCACGATGCC GCTGGGCTGA ATAATCGGGA TCAGGGCCCG GGCGCGCTCG GTGAGCTGCG GATGTGCGGC AAAGAAAGCC TCGACCTGCC GGGCGCCTGT 1300
CATGCCGAGT CCCTCGATGG CTTTCCACCA GCCACGCCGG CGGGGAATGC GCACGGTCAG CGCCGAGAGG GTGTGGATAC CGTACGCCTG CAGTGCCCTG 1400
ACGGTCCTCG GAGACAGCCA CTGGTCGATG CTGTCGCTGA TCTGGGGCTT CGGCGCCGGC AGCGTGGGCA AGAGCCGGAT CGCGCGTTCG ACGGCACCCG 1500
CGCGCCCCTG TCGCTCTTCG GGGGCGTGCT GGAACACGTC GGCCAGATCC GGCCGGTGGC GGCTGTTGGC GAAAACGATG AGCTGGCGGC GGATATGCCC 1600
CAGCATCCCT CGCGCTGATT GACCGGCGGC CTTGCGTGCC GCCAGGTAGT GTTCAACGGC CGCGCGAGAG CTGAGTCCCG CGTACCAGCC GCGCAAGGCA 1700
GCCAGCGAAT CCGCATCGGG AAAGCCGACT GGGGTAGAAC TCGAAGCCGT AGTCTGGCCC GTTTTCATAT GGACAGGTTG CGATTCGAAA AGCGAGTAAC 1800
GCGATAATAA CGATTATTTC AGTAGAATCG AAAATGCCCC ATGTTTCTAC GAAACCTCAA GATAGCAAGA ACGGGCCATG AGCAAGAAGA ACAAGCTACT 1900
CAGCGTCCTA TCCGACGCCG AACAGGAGGC CTTGTATGGC CTGCCGGAGT TCGATGATGC GCAGCAGCTG GAATATCTGG CTGTGACAGA AACCGAGTTG 2000
GCGCTTGCCA ACAGCCGGCC CAGTCTTCAT GCCAAGGTCT ATTGCCTCTT GCAGATCGGC TACTTCAAGG CCAAGCATGC CTTCTTCAGT TTCGACTGGG 2100
ACGAAGTCGA GGACGATTGC GCTTTCGTGC TGAGCCGCTA TTTCCACGGC GAGGTGTTCG AACCCAAGGC GATCACCAAG CATGAACGCT ACACCCAGCG 2200
CGAGCAGATC GCCAAACTAT TCGGCTACTG GCCGTGGTCG GCCGCCTTCC TGCCGCAGCT CGAGCAGCAG GCCGCACGGA CCGTGCGGCG TGACGTAACG 2300
CCGGGGTTCG TCGCCGCCGA GCTGATCGTC TGGCTCAATG AGCACAAGAT TATCCGGCCC GGCTATACGA CCTTGCAAGA GTTGGTCAGC GAAACGCTAT 2400
CCGCCGAGCG CCGTGACGGA AAGCTTACAA AAACTGACAC TGGGCTCTGC ACTGGCTGCG CCGCAGCGGC AGCAGTTTGT TGATTGGCTA AAGGGAAACA 2500
CGACCGGCAA CCACCGCATC CGCGCGGCGG TGCCGGCAGA CTGGGCAGTC GGAGACAAAA CCGGAACCTG CGGAGTGTAT GGCACGGCAA ATGACTATGC 2600
CGTCGTCTGG CCCACTGGGC GCGCACCTAT TGTGTTGGCC GTCTACACCC GGGCGCCTAA CAAGGATGAC AAGTACAGCG AGGCCGTCAT CGCCGCTGCG 2700
GCTAGACTCG CGCTCGAGGG ATTGGGCGTC AACGGGCAGT AAGGCTCTGA AAATCATCTA TTGGCCCACC ACCGCCGCCC TTGCGGGCGG CATGGATTAC 2800
CAACCACTGT CACATTTAGG CTAGGAGTCT GCGCGGCAGA GCCGTGTGAC CGGTTTTCTG TAGAGCACTG ACGATGGCGG CGGCGCTCTC TGCAATTGGC 2900
AAGGCGTCGG CGCCAAGGAT ACCAATCTTG CGGCGCGCGG CGTGTTATGA CGACTGGGGT GCATTTGAGC CGCCCCATTT AACCTTCGCC CTCACAGATA 3000
CGCCATTCGC CTCAGATTTA GCGCCATGCA GACGAGCTTC CACTCGGCTT GCACCTTGTC CAGGCCCCTC ATGCTGAACT GACGCAATCC CATCACCGCC 3100
TTGATCCAAC CATTCGGAGC CTCCACGATC GACTTGCGCC GGCGGTAAGC TGCATCGCCT TGCTCCGTTT TCAATTTCGC CGCAATCGCC GCCGTATGCG 3200
GATGGGTCTT GGCATTGACC TTGGCATCTT CACGTCCCTC GCGGCCGAGG GCAACGATGA CATCGCCGTG GTGATCGGCG ACCTTTGCCA GAACAGCCTC 3300
ACTACGGAAT CCCGCATCCG CCAGCGTCTG GGCCGGCATT TCTCCGGTGT TGGCCTGAAC TGCTGCCAGC ATGCCCAGCA GCGCCTGACT GTCCGCGGCG 3400
CAGTTGGTCA ACTCCGCCGC CACGATGATC TGGTGCTCGG CATCGACCGC TGTGTACCCG TTGTAGCTCT GCTCGGAGCC ACCACCGGCG TGTTTCATGA 3500
TCCGGCTGTC CGGATCGGTG AAGCTTTCCT GATCACGGTC ATCCGGCACA CCAAACTCGC GTTTGTACGA GCCACCGCCC TTGTCCGAGC CATCCGGATG 3600
GCGAGGCCGG CGGCCATCGT CTTCGCTGCG CCCCCGGGCC TGGTCCGCTT CACGCTGGCG CGCTTCCAGG CGCGCCTTTG CCGCCTGGAT CGCCTCCAGG 3700
CGCTTCTCGC GGCGAGAAAT CTCGGCAGGA ATGTCCAGCT CCGGCTCGTT ACGCTCCTGG TCGTCGGTAG CCTTGGCGCG ATCAAGCAGC GCCTTGATCT 3800
CGCAATGCAA TTCGTCCTCG GCCGGCTTCA TGCGCTTATA GCTCATCGCC TTGTGGCGGC TGGCGTTGGC TTTTACCTTG GTGCCGTCGA CCGCGATCGT 3900
GCCAAGCTTC ACCAGCCCAC ATTCGCGCGC CAGTTGCACC ACCTGAACGA ACAAATTCTC AAGCTCGGTC AGGTGTAGGG CACGGAAGTC ACTCAGCGTG 4000
CGGTGGGCCG GGAAGTTTCC AGCGGCCAGC ACACGCAACG CGACATCCTC GTACAGCTTC CTGGCTAGTT TGCGCGAAGA GAAGACGCCG CTCGCGTAAC 4100
CATAGATCAG CACTTTGACC ATCATCGCCG GATGAAACGG CTGATTGCCC GGACCACCGC CGGCATACCG GGCGTGGAAT GCGCTCAAGT CCAGCGTATC 4200
GACAGTCTCG CTGATGAAGT AGGCAAGATG CCCTTCAGGT AGCCACTCTC CCAGAGAAGG GGGCAGCAGA TAGGATTGGT CGGGTCGGTA AGGAAGGTAA 4300
CTGGCAGCCA TCCCCGTATC GTCCTCGATC TTCCGCCGAT TTGGCTTCTG CCGCGCAGGC TCCTAGGCCA CCGTAGCTAA ACATACGAGA AAAACCGCTT 4400
CTTGAGCGCT CTCTGAGACC ACCTTGCCGA CAACGCAGAA ACGGTTGCGG AAAAATCGGC GTTTCCGGCG TACGCCACCC GACCTGGACG AACTATTGCG 4500
GACCTGGAAC TGGAGTAGCG GAATTCCCCG GGGTTTCAGC GTCGCGCCAC AGCGCCTCAG ATAGATGCGG TAGCCTTTGG CGGGTCGTCA TGCCGCGGAC 4600
CACCCCCCCA AAAAATTCCA CAATGCGGAA TGGCGGAATT TTCTGGGGTT CCCGCTTACC CCCCC
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| ORFs |
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| Gene Name |
Associated TE |
Coordinates |
Class |
Sub Class |
Orientation |
| tnpR |
Tn4401d |
53-1768 |
Accessory Gene |
Resolvase |
- |
| tnpA_p |
Tn4401d |
1878-2413 |
Transposase |
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+ |
| bla KPC-3_p (ARO:3002313) |
Tn4401d |
2414-2742 |
Passenger Gene |
Antibiotic Resistance |
+ |
| tnp |
ISKpn6 |
2992-4311 |
Transposase |
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnpR |
TnpR |
Tn4401d |
1716 |
53-1768 |
- |
| Class: | Accessory Gene |
| Sub Class: | Resolvase |
| Transpoase Chemistry: | Serine |
| Sequence Family: | Serine Site-Specific Recombinase |
Protein Sequence:
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MKTGQTTASS STPVGFPDAD SLAALRGWYA GLSSRAAVEH YLAARKAAGQ SARGMLGHIR RQLIVFANSR HRPDLADVFQ HAPEERQGRA GAVERAIRLL
PTLPAPKPQI SDSIDQWLSP RTVRALQAYG IHTLSALTVR IPRRRGWWKA IEGLGMTGAR QVEAFFAAHP QLTERARALI PIIQPSGIVP WEQLRLPNEV
DGSQGNFRAP RNACTLNATN DYEAVQAWLA LHETVATQRA YRKEAARLIL WAIVERGRAL SSLTTDDAIA YRAFVRHPTP RERWVGPPRP RDSVEWRPFS
GGLSARSAAY ALTVLSALFR WLIEQRYVLA NPFAGVKVRG HALRPALDTA RGFTEGEWLL LRAIADGLEW SYGWSEPAAQ RLRFMLDFGY ATGLRASELV
GAVLGNVRLD EHGDHWLHLV GKGGKPGKVA LPPLAYTALD QYLAQRQLPV SRERWNPKTP LVGSLGEDGN AGITGPHLWC VLRRFFTQAA EAIEADHPVA
AEKLRRATPH WMRHSHASHA LARGAQLTAV RDNLRHASIA TTSMYLHGDE LERARQMRQA FGARHVKRGV D
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnpA_p |
N |
Tn4401d |
536 |
1878-2413 |
+ |
| Class: | Transposase |
| Transpoase Chemistry: | DDE |
| Comment: | fragment consisting of N-terminal amino acids 1 to 178 |
Protein Sequence:
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MSKKNKLLSV LSDAEQEALY GLPEFDDAQQ LEYLAVTETE LALANSRPSL HAKVYCLLQI GYFKAKHAFF SFDWDEVEDD CAFVLSRYFH GEVFEPKAIT
KHERYTQREQ IAKLFGYWPW SAAFLPQLEQ QAARTVRRDV TPGFVAAELI VWLNEHKIIR PGYTTLQELV SETLSAER
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| bla KPC-3_p (ARO:3002313) |
Bla KPC-3_p |
Tn4401d |
329 |
2414-2742 |
+ |
| Class: | Passenger Gene |
| Sub Class: | Antibiotic Resistance |
| Function: | antibiotic inactivation (ARO:0001004) |
| Target: | cephalosporin (ARO:0000032)||monobactam (ARO:0000004)||penam (ARO:3000008)||carbapenem (ARO:0000020) |
| Sequence Family: | KPC beta-lactamase (ARO:3000059) |
| Comment: | fragment of KPC-3 missing 553bp from 5'-end |
Protein Sequence:
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*RKAYKN*HW ALHWLRRSGS SLLIG*RETR PATTASARRC RQTGQSETKP EPAECMARQM TMPSSGPLGA HLLCWPSTPG RLTRMTSTAR PSSPLRLDSR
SRDWASTGS
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnp |
Tnp |
ISKpn6 |
1320 |
2992-4311 |
- |
| Class: | Transposase |
| Transpoase Chemistry: | DDE |
Protein Sequence:
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MAASYLPYRP DQSYLLPPSL GEWLPEGHLA YFISETVDTL DLSAFHARYA GGGPGNQPFH PAMMVKVLIY GYASGVFSSR KLARKLYEDV ALRVLAAGNF
PAHRTLSDFR ALHLTELENL FVQVVQLARE CGLVKLGTIA VDGTKVKANA SRHKAMSYKR MKPAEDELHC EIKALLDRAK ATDDQERNEP ELDIPAEISR
REKRLEAIQA AKARLEARQR EADQARGRSE DDGRRPRHPD GSDKGGGSYK REFGVPDDRD QESFTDPDSR IMKHAGGGSE QSYNGYTAVD AEHQIIVAAE
LTNCAADSQA LLGMLAAVQA NTGEMPAQTL ADAGFRSEAV LAKVADHHGD VIVALGREGR EDAKVNAKTH PHTAAIAAKL KTEQGDAAYR RRKSIVEAPN
GWIKAVMGLR QFSMRGLDKV QAEWKLVCMA LNLRRMAYL
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| Internal Transposable Elements (TE) |
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| TnCentral Accession |
TE Name |
Type |
Coordinates |
Length |
| ISKpn6-KT378596 |
ISKpn6 |
Insertion Sequence |
2824-4363 |
1540 |
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| Internal Repeat Elements |
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| Name |
Associated Mobile Element |
Coordinates |
Sequence (Top Strand) |
| IRR |
ISKpn6 |
2824-2839 |
GGAGTCTGCG CGGCAG |
| IRL |
ISKpn6 |
4348-4363 |
GACGGCGCGT CCGAGG |
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| References |
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Chen L, Chavda KD, Mediavilla JR, Jacobs MR, Levi MH, Bonomo RA, Kreiswirth BN. Partial excision of blaKPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae. Antimicrob Agents Chemother. 2012 Mar;56(3):1635-8. doi: 10.1128/AAC.06182-11. Epub 2011 Dec 27. PubMed ID: 22203593
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