Transposon
Name: Tn4352
Family: Compound Transposon        Group: IS26
Evidence of Transposition: no
 Host     

Host Organism:Salmonella typhimurium Molecular Source:plasmid NTP16
Place of Origin:Middle East Date of Isolation:1987
Other Geographic Information:clinical isolate

 Map     



 Terminal Inverted Repeats (IR)     


 Sequence     
DNA SequenceLength  2704 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
TTTGCCCCAT ATCACCGTCG GTACGGCACT GTTGCAAATA GTCGGTGGTG ATAAACTTAT CATCCCCTTT TGCTGATGGA GCTGCACATG AACCCATTCA 100
AAGGCCGGCA TTTTCAGCGT GACATCATTC TGTGGGCCGT ACGCTGGTAC TGCAAATACG GCATCAGTTA CCGTGAGCTG CAGGAGATGC TGGCTGAACG 200
CGGAGTGAAT GTCGATCACT CCACGATTTA CCGCTGGGTT CAGCGTTATG CGCCTGAAAT GGAAAAACGG CTGCGCTGGT ACTGGCGTAA CCCTTCCGAT 300
CTTTGCCCGT GGCACATGGA TGAAACCTAC GTGAAGGTCA ATGGCCGCTG GGCGTATCTG TACCGGGCCG TCGACAGCCG GGGCCGCACT GTCGATTTTT 400
ATCTCTCCTC CCGTCGTAAC AGCAAAGCTG CATACCGGTT TCTGGGTAAA ATCCTCAACA ACGTGAAGAA GTGGCAGATC CCGCGATTCA TCAACACGGA 500
TAAAGCGCCC GCCTATGGTC GCGCGCTTGC TCTGCTCAAA CGCGAAGGCC GGTGCCCGTC TGACGTTGAA CACCGACAGA TTAAGTACCG GAACAACGTG 600
ATTGAATGCG ATCATGGCAA ACTGAAACGG ATAATCGGCG CCACGCTGGG ATTTAAATCC ATGAAGACGG CTTACGCCAC CATCAAAGGT ATTGAGGTGA 700
TGCGTGCACT ACGCAAAGGC CAGGCCTCAG CATTTTATTA TGGTGATCCC CTGGGCGAAA TGCGCCTGGT AAGCAGAGTT TTTGAAATGT AAGGCCTTTG 800
AATAAGACAA AAGGCTGCCT CATCGCTAAC TTTGCAACAG TGCCAATTTA TGAGTAAAGG ATTATGTCCA CGATAAGCAC CTGGGTCGAT TCCTGGGAGG 900
CGGCCATGAG GGTAGGGAAG CGCCGTCCCG TCAAGTCAGC GTAATGCTCT GCCAGTGTTA CAACCAATTA ACCAATTCTG ATTAGAAAAA CTCATCGAGC 1000
ATCAAATGAA ACTGCAATTT ATTCATATCA GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG AGGCAGTTCC 1100
ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA 1200
GAAATCACCA TGAGTGACGA CTGAATCCGG TGAGAATGGC AAAAGCTTAT GCATTTCTTT CCAGACTTGT TCAACAGGCC AGCCATTACG CTCGTCATCA 1300
AAATCACTCG CATCAACCAA ACCGTTATTC ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGC TGTTAAAAGG ACAATTACAA ACAGGAATCG 1400
AATGCAACCG GCGCAGGAAC ACTGCCAGCG CATCAACAAT ATTTTCACCT GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTCC CGGGGATCGC 1500
AGTGGTGAGT AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC GTCAGCCAGT TTAGTCTGAC CATCTCATCT 1600
GTAACATCAT TGGCAACGCT ACCTTTGCCA TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA ATCGATAGAT TGTCGCACCT GATTGCCCGA 1700
CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA TTTAATCGCG GCCTCGAGCA AGACGTTTCC CGTTGAATAT GGCTCATAAC 1800
ACCCCTTGTA TTACTGTTTA TGTAAGCAGA CAGTTTTATT GTTCATGATG ATATATTTTT ATCTTGTGCA ATGTAACATC AGAGGGCACT GTTGCAAATA 1900
GTCGGTGGTG ATAAACTTAT CATCCCCTTT TGCTGATGGA GCTGCACATG AACCCATTCA AAGGCCGGCA TTTTCAGCGT GACATCATTC TGTGGGCCGT 2000
ACGCTGGTAC TGCAAATACG GCATCAGTTA CCGTGAGCTG CAGGAGATGC TGGCTGAACG CGGAGTGAAT GTCGATCACT CCACGATTTA CCGCTGGGTT 2100
CAGCGTTATG CGCCTGAAAT GGAAAAACGG CTGCGCTGGT ACTGGCGTAA CCCTTCCGAT CTTTGCCCGT GGCACATGGA TGAAACCTAC GTGAAGGTCA 2200
ATGGCCGCTG GGCGTATCTG TACCGGGCCG TCGACAGCCG GGGCCGCACT GTCGATTTTT ATCTCTCCTC CCGTCGTAAC AGCAAAGCTG CATACCGGTT 2300
TCTGGGTAAA ATCCTCAACA ACGTGAAGAA GTGGCAGATC CCGCGATTCA TCAACACGGA TAAAGCGCCC GCCTATGGTC GCGCGCTTGC TCTGCTCAAA 2400
CGCGAAGGCC GGTGCCCGTC TGACGTTGAA CACCGACAGA TTAAGTACCG GAACAACGTG ATTGAATGCG ATCATGGCAA ACTGAAACGG ATAATCGGCG 2500
CCACGCTGGG ATTTAAATCC ATGAAGACGG CTTACGCCAC CATCAAAGGT ATTGAGGTGA TGCGTGCACT ACGCAAAGGC CAGGCCTCAG CATTTTATTA 2600
TGGTGATCCC CTGGGCGAAA TGCGCCTGGT AAGCAGAGTT TTTGAAATGT AAGGCCTTTG AATAAGACAA AAGGCTGCCT CATCGCTAAC TTTGCAACAG 2700
TGCCTTCTGG AACAGGAGAC CGGGCTGAAT

 Recombination Sites     

Name Coordinates Gene Sequence
res_core_IRR 364-371 8 CGGGCCGT
res_core_IRR 2224-2231 8 CGGGCCGT

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
tnpA IS26 88-792 Transposase   +
APH(3')-Ia (ARO:3002641) Tn4352 982-1797 Passenger Gene Antibiotic Resistance -
tnpA IS26 1948-2652 Transposase   +

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA IS26 705 88-792 +
Class:   Transposase
Transpoase Chemistry:   DDE
Protein Sequence:  
MNPFKGRHFQ RDIILWAVRW YCKYGISYRE LQEMLAERGV NVDHSTIYRW VQRYAPEMEK RLRWYWRNPS DLCPWHMDET YVKVNGRWAY LYRAVDSRGR
TVDFYLSSRR NSKAAYRFLG KILNNVKKWQ IPRFINTDKA PAYGRALALL KREGRCPSDV EHRQIKYRNN VIECDHGKLK RIIGATLGFK SMKTAYATIK
GIEVMRALRK GQASAFYYGD PLGEMRLVSR VFEM

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
APH(3')-Ia (ARO:3002641) APH(3')-Ia Tn4352 816 982-1797 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic inactivation (ARO:0001004)
Target:   aminoglycoside antibiotic (ARO:0000016)
Sequence Family:  APH(3') (ARO:3000126)
Comment:   strict match to reference sequence for ARO:3002641 (bitscore: 560)||Synonyms: aphA-1, apha1-1AB, APH(3')-Ic, apha7
Protein Sequence:  
MSHIQRETSC SRPRLNSNMD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP ELFLKHGKGS VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA
IPGKTAFQVL EEYPDSGENI VDALAVFLRR LHSIPVCNCP FNSDRVFRLA QAQSRMNNGL VDASDFDDER NGWPVEQVWK EMHKLLPFSP DSVVTHGDFS
LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS PSLQKRLFQK YGIDNPDMNK LQFHLMLDEF F

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA IS26 705 1948-2652 +
Class:   Transposase
Transpoase Chemistry:   DDE
Protein Sequence:  
MNPFKGRHFQ RDIILWAVRW YCKYGISYRE LQEMLAERGV NVDHSTIYRW VQRYAPEMEK RLRWYWRNPS DLCPWHMDET YVKVNGRWAY LYRAVDSRGR
TVDFYLSSRR NSKAAYRFLG KILNNVKKWQ IPRFINTDKA PAYGRALALL KREGRCPSDV EHRQIKYRNN VIECDHGKLK RIIGATLGFK SMKTAYATIK
GIEVMRALRK GQASAFYYGD PLGEMRLVSR VFEM

 Internal Transposable Elements (TE)     

TnCentral Accession TE Name Type Coordinates Length
IS26-MH257753 IS26 Insertion Sequence 25-844 820
IS26-MH257753 IS26 Insertion Sequence 1885-2704 820

 Internal Repeat Elements     

Name Associated Mobile Element Coordinates Sequence (Top Strand)
IR IS26 831-844 AAACGTTGTC ACGG
IR IS26 1885-1898 GGCACTGTTG CAAA

 References     

1.Wrighton CJ, Strike P. A pathway for the evolution of the plasmid NTP16 involving the novel kanamycin resistance transposon Tn4352. Plasmid. 1987 Jan;17(1):37-45. doi: 10.1016/0147-619x(87)90006-0. PubMed ID: 3033719
2.Anderson ES, Threlfall EJ, Carr JM, McConnell MM, Smith HR. Clonal distribution of resistance plasmid-carrying Salmonella typhimurium, mainly in the Middle East. J Hyg (Lond). 1977 Dec;79(3):425-48. doi: 10.1017/s0022172400053286. PubMed ID: 336792
3.Lambert CM, Wrighton CJ, Strike P. Characterization of the drug resistance plasmid NTP16. Plasmid. 1987 Jan;17(1):26-36. doi: 10.1016/0147-619x(87)90005-9. PubMed ID: 3033718