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Recombination Sites | |
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Internal Transposable Elements | |
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Internal Repeats | |
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| Name: Tn3000 (Synonyms: IS3000) |
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| Family: Tn3 Group: Tn3000 |
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| Evidence of Transposition: no |
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| Host Organism: | Escherichia coli | Molecular Source: | plasmid pMSP071 |
| | Date of Isolation: | 1999 |
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| Map |
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| Terminal Inverted Repeats (IR) |
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| IRL (Length: 50 bp) |  | GGGGTTCTAGGGATTTTCCGTCCAAAAACGACAAAGTGCTCTGGAGGCCC |
| IRR (Length: 50 bp) | | GGGGTTCTAGGGATTTCCGTCCAAAAACGACAAAGTGCTCTGGAGGCCGC |
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--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
GGGGTTCTAG GGATTTTCCG TCCAAAAACG ACAAAGTGCT CTGGAGGCCC CGCCGTCTGT GGCCTCCAGA GGGGTATTAC TTTTTTCGAG ATCCCTATTT 100
GGGCTCTGTT TGAGTAAGAT TTAGTCTTTT TGGGCTAAAT CCATGGCATT CGAAGAACGT GTCCAAATCT TGTCCGAGGC AGAACAAGAT GAGTTATATG 200
GACCCCCCGC TTTCACCTCC GCCGACCAAC GGTTCTTTTT CTCGCTGAAC GATAAGGAAC TGGCGATCGC TAAAAGCCTC CGTCATCGGG GCCAGCGTTA 300
CATGCTGGTG GTACTGCTGG GCTATTTCAA AGCCAAGCCG GTGGTGCTGA ATCCCGGCTT TCATCAGATC AAGCAGGACC TCAAATACGT TTATCAAACC 400
GTTCTACCAG GCCCAGGCTG TAGACCCTTT AATCTCACGC CAAAAGAGAA CGAACGGATT TACCAACGTG TTTTCCAGCT CTGCAACTAC CAGCGCTGGA 500
ATGTCAAAGA CCATGGAGCG GCGTTGAGAG ATTACTTATC CCAGCAAGCC AGAGCCTGGA CAGCGCCTAG GCACCTCTTC GATGCGGCCA TTGAATATTG 600
TTCGGGGCAG AAGATCGCAA TCCCTGCCTA CAGCACGCTG CAAAAGATCA TCAGTCAGGT GGTGGGAGAC GAACAGGAAC ACATGGCCGC GCACCTTGAG 700
CGTGCAATGT CACGCGGTCT TAAGCAAGCA CTGGCGGAGC TCGTGAACGG CACAGGTCCA CTGCCGTTCC GACAGTTGCG ACAATCGGCT CGTAACTTCA 800
CCGGAACCGA GTTGGAGAAA GAACTGATTG TCTATCGCCA TATCCAGCAT TGGATGCCGG AAGTGGATCT GCTGTTGAGC ACGCTATCAC TGTCGCAGAA 900
AAATCTGCAA CACCTGGCAG AGAAAGTCGA CTACTACGGT GCCAAACTGA AACGTCAAAC CGTAGGCAGC CAATGGCTGT ATTTATTGTG CTATCTTCAA 1000
ACGCGATGGC AGCAAGCGCT GGAACGTATT GCCGATGGCT TTGTGCATCA TGTCAGACAG ACCAAACAGA AGGCAAAGGA TTATGCGCAG GAAGCGGTGT 1100
TTAAGGATTG GCAAAAAGCA GCTAAAAATG TCAGCAAGGC AGCTGAAGTA CTGCACCTGT TCATTGATGA CAGCATTGAT CTGCAACTAC CGTTTGCAAC 1200
AGTAAGACAG CAAGCACTGA GCCTGCTGAC CAAAAGGGAT CTGGAATCTG TATGTCTCTT CCTGAACGAG CAGCGACGAT CGGTCGATGA AGCCATGTGG 1300
CAGTACTGCG ACGAGAAAGA AAGTCTGCGG AAAGGTTTGC TGCGAGAGTT GTTCCTATGT CTGCGCTTCG AAGGCTGCGA CGGCACCCAG CACTTAGCGG 1400
CCGCCTTGGC GAAAACACAA AACGAACTCA ACGGCCAGGA CGCTCAGTTG CAAACTGCCG ACACCAGACT CCTTTCCAAA AAATCACGTG AATTCCTGCT 1500
GGATGGTGAA GGGAATATCC TGATCGATCG TTATGAGTGG TTCCTCTATC AACAGATTCC TGATCGCCTG AATGGCCAGC TGACGGTGCC TGATATCACT 1600
AAATACCGGG CACTCGACGC CGACCTGATC GACGGGGAAC ATTGGCGAAA AAACAAATAT ACGCTGCTTC AACAGAGCCA TTTTACAAAA TTAGCGGAGG 1700
AGCCTGAAAA GCTGATCAAA CAGATGGCCA TGGAATTGGA TACCCGTTTG TACGAAGTCG GCGAATATCT TGAACAGGAA GACAACCGGA ATATCATCTT 1800
GCGTAATCCG CAGGGTAAAC GCTTCTGGCG CCTGCCTTCG GCCAGCAAAC ATCATCTGGT CAACAATCCC TTCTTCCAGC AAATTCCCAC AACGGGGATT 1900
GCGGATGTAC TGCGCATGGT TGATCGTGAC ACCGGTTTCA TTGACTGCTT CGCTCATGTG CTGGGTTCCC AATCCAGAAG CCGTTCCCAT GAATATGACC 2000
TGTTGGCAAT TCTGGTCGGC AATGCAACCA ATCAAGGCAT TTACGGCATG GCACAGATCT CTGATCGTAC CTATGATCAG CTCAGCACTA TCCAGGCGAA 2100
CTATCTTCGC CTGGAAACAT TGAATGCTGC TAACGACAAC ATCAATAACG CGACAGCCAA GCTACCCATC TTCCGGTACT ACAACATCCA GGAAGATGTG 2200
ATCCACGCCA GTGCCGATGG TCAAAAGTTC GAAGCCCGGC GCGAGACCTT CAAAACCCGT TATTCGTCGA AGTACTTTGG CACTCAAAAA GGTGTTTCTG 2300
CCATGACCTT GATCGCCAAT CACGCTGCGA TCAACGCCAG AGTGATCGGC GCCAACGAAC ATGAATCGCA CTACATCTTT GATTTGTTGA TGAGCAATAC 2400
GTCAGACATC ATTCCGGATG TGCTCTCAAC CGATACCCAT GGGGTGAACC ATGTGAACTT CGCGTTACTG GATCTGTTCG GATACCAGTT TGCCCCACGC 2500
TATGCCCAGG TTGGCAAAGT GATCAATGAC ATGTTTGATG TCAAGGAAGA CAAAGAACAC CGAATTCAGC TGTGCTTAAA AAAGCCAATC AATACCCATC 2600
GTATTGCGCA GCACTGGGAT ACCATCCAAC GGATTGCAGT ATCACTTAAG CAGCGGAAAA CAACGCAAGC CACCTTGGTG AGAAAGCTCT CGGAGTACAA 2700
GCGCAATCAC CCGCTGCTGG AAGCCCTGAC GGAATACAAT CGCCTGGTGA AAGCGAATTA TCTACTGTGC TACATCGATG ATGCCAGTTT AAGAAACTAT 2800
GTTCAGCGCG CGCTGAACCG GGGAGAGGCC TATCACCAAC TGCGTCGGCC CGTGAGCAGC GTCAATGGGG ATCAGTTCCG GGGCAGTTCA GACGAAGAAA 2900
TCCAGCTATG GAATGAGTGC GCTCGCCTGG TCACCAATGC CATCGTTTAC TTCAACTCCA GGATACTCAG CCAGCTGTTG ACCAGCTTCG AATACCAAGG 3000
AGATACCAAG AGAATAGATA TCGTCAAACA GGCATCCCCT GTGGCCTGGC ACAACATTAA CCTCAAGGGG ACTTACCACT TCGAATTGAG CGAAAAATTG 3100
CCAGATCTGG AGGAGCTTAT GCGCTCAATC GAGGGATATT TACCCGTCAG CGAAAAGTAA TACCCCTCTG GAGGCCACGG ACGGCGCGGC CTCCAGAGCA 3200
CTTTGTCGTT TTTGGACGGA AATCCCTAGA ACCCC
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| ORFs |
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| Gene Name |
Associated TE |
Coordinates |
Class |
Sub Class |
Orientation |
| tnpA |
Tn3000 |
143-3160 |
Transposase |
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnpA |
TnpA |
Tn3000 |
3018 |
143-3160 |
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| Class: | Transposase |
| Function: | transposase |
| Transpoase Chemistry: | DDE |
Protein Sequence:
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MAFEERVQIL SEAEQDELYG PPAFTSADQR FFFSLNDKEL AIAKSLRHRG QRYMLVVLLG YFKAKPVVLN PGFHQIKQDL KYVYQTVLPG PGCRPFNLTP
KENERIYQRV FQLCNYQRWN VKDHGAALRD YLSQQARAWT APRHLFDAAI EYCSGQKIAI PAYSTLQKII SQVVGDEQEH MAAHLERAMS RGLKQALAEL
VNGTGPLPFR QLRQSARNFT GTELEKELIV YRHIQHWMPE VDLLLSTLSL SQKNLQHLAE KVDYYGAKLK RQTVGSQWLY LLCYLQTRWQ QALERIADGF
VHHVRQTKQK AKDYAQEAVF KDWQKAAKNV SKAAEVLHLF IDDSIDLQLP FATVRQQALS LLTKRDLESV CLFLNEQRRS VDEAMWQYCD EKESLRKGLL
RELFLCLRFE GCDGTQHLAA ALAKTQNELN GQDAQLQTAD TRLLSKKSRE FLLDGEGNIL IDRYEWFLYQ QIPDRLNGQL TVPDITKYRA LDADLIDGEH
WRKNKYTLLQ QSHFTKLAEE PEKLIKQMAM ELDTRLYEVG EYLEQEDNRN IILRNPQGKR FWRLPSASKH HLVNNPFFQQ IPTTGIADVL RMVDRDTGFI
DCFAHVLGSQ SRSRSHEYDL LAILVGNATN QGIYGMAQIS DRTYDQLSTI QANYLRLETL NAANDNINNA TAKLPIFRYY NIQEDVIHAS ADGQKFEARR
ETFKTRYSSK YFGTQKGVSA MTLIANHAAI NARVIGANEH ESHYIFDLLM SNTSDIIPDV LSTDTHGVNH VNFALLDLFG YQFAPRYAQV GKVINDMFDV
KEDKEHRIQL CLKKPINTHR IAQHWDTIQR IAVSLKQRKT TQATLVRKLS EYKRNHPLLE ALTEYNRLVK ANYLLCYIDD ASLRNYVQRA LNRGEAYHQL
RRPVSSVNGD QFRGSSDEEI QLWNECARLV TNAIVYFNSR ILSQLLTSFE YQGDTKRIDI VKQASPVAWH NINLKGTYHF ELSEKLPDLE ELMRSIEGYL
PVSEK
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| References |
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| 1. | Sabate M, Tarrago R, Navarro F, Miro E, Verges C, Barbe J, Prats G. Cloning and sequence of the gene encoding a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-9) from Escherichia coli in Spain. Antimicrob Agents Chemother. 2000 Jul;44(7):1970-3. doi: 10.1128/aac.44.7.1970-1973.2000. PubMed ID: 10858363
| | | 2. | Sabate M, Navarro F, Miro E, Campoy S, Mirelis B, Barbe J, Prats G. Novel complex sul1-type integron in Escherichia coli carrying bla(CTX-M-9). Antimicrob Agents Chemother. 2002 Aug;46(8):2656-61. doi: 10.1128/aac.46.8.2656-2661.2002. PubMed ID: 12121950
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