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Internal Transposable Elements | |
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Internal Repeats | |
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| Name: Tn163 |
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| Family: Tn3 Group: Tn4430 |
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| Evidence of Transposition: no |
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| Host |
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| Host Organism: | R. leguminosarum bv. viciae strain E163N | Molecular Source: | plasmid pRleE163Na |
| Place of Origin: | Zentrum ffir Agarlandschafts-und Landnutzungsforschung, Muincheberg, Germany | Date of Isolation: | 1994 |
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| Map |
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| Terminal Inverted Repeats (IR) |
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| IRL (Length: 38 bp) |  | GGCCCCCGAACATTAAAGGGGCACAGATATACGGGATG |
| IRR (Length: 38 bp) | | GGCCCCTGAACATTAAAGGGGCGCAGATATACGGTAGG |
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| Sequence |
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--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
GGCCCCCGAA CATTAAAGGG GCACAGATAT ACGGGATGGC TTTCATTTCT GGTCAGCCGA GTGCTGACAA CAGCCGATCA CGATTTTTCT GAACGTACTC 100
TATGTCCCTG AGGTATATGG CGGCGTCGCC GGCCTCCACG TCCTCCACGA AGGTTGAGTC TCCTGTTTCT GCTCTTGAAT GCATGAAATC CACCAATGCT 200
TGCAGTCGTC CGCAGATCGC CTCGGGAAGC CTGCGCTTTT CGTCGGCTGT CGCGCCATAG GATGCACAAA AGATGCTTGC GCGATGCAAT TGTTCACGGA 300
CATCGAACAC AACGCCATGG TTGGAATAGT CAGACAATGG CGCCCATCTG TAAACTGCGT AGGCCAGATC CCATAAGGCT GGCGCCGGAT GTGCGGTGTC 400
GAAATCAATG ATCCCGACAG CCGCATCGCC CTCAGTTGCG ACATTGTAGG GGGCGAAGTC ACCGTGGCAG ATAATCTCGC GAGGTTCTTG TGGCGGCAGC 500
ATCCACATTT GGATCTCGCG ATCAACGTCC AGGAACCCAC GCGAAGCTGA ATGGAATTTA CGCAGAAGCT CGCCTGCAGA GCGTAGCATC CGCTCCGAAC 600
CAACAAGTCG ATCCGCCAAA TGCTCACAAA CGCGCCCTTT GACGTAAGTT ACGGTTTCTT GATGTTGCGT TAGTTCGACA GGAATTGGTG CAGCAGAAAA 700
GCCGCTGTTT TGCAAATGGC GCAAAAACCT ATGTACTGTA GGCGTCCAAG GACCAGATGG CCTCCTGACG GTATTTCCGT CGCGCCAAAT CTGCCCTGCT 800
CGCCCTCCCT CCAATTCTTT CATTTTCTTA CTCCCAAAGT GATCTTTTTC CGTCCGAGCA GACGTTCACC ATCGCCCCGC AACTCACCCT TGGGACCTAC 900
AAACCGGTAT AAGGTCTGAC GGGTGATGCC GAGTTCTGCG CAGAGATCGG CCACCTTCGT CTCAGGTTTT CCCATCATTG CTTGAGCAAG GCGTAATTTC 1000
GCAGGCGTCA TTTTGAATGG CGCCCCGCCA TTCCGGCCAC GCGCTCGGGC TGCCGCTAGG CCTGCTTTGG TCCGCTCGAT GATCAGTGCC CGCTCAAATT 1100
CGGCAAGAGC GGCAAATATT CCGAACACCA GCCGGCCGTT CGCTGTGGAA GTGTCGATCG ATGCGCCTTC GCCTGCGAGT ACTTTCAGAC CGATGTTCCT 1200
CTTTGTGAGG TCGTCCACGA GATTGACGAG GTGACGCAGG TCACGACCGA GACGGTCAAG CTTCCAGACG GCGAGCGTGT CGCCAGCGCG CAGCTTCAAG 1300
CAAGCCGCTA GACCGGGACG ATCATCTTGC TTGCCCGAGG CGGAATCCTC ATAGACGTTA TCGACCTCGA CGCCAGCCTT GATCAGCGCA TCGCGCTGGA 1400
GGTCATGGAC CTGGCTGCCG TCGGCTTTCG ACACCCGCGC ATATCCGATC AGAGCTGTCA TTTATACGTC CGTTTGTGTG ACATTCTTCA TTTGGTCGTG 1500
CCAAAGTCTG TAGATTGTCA CATAACCCGT CTTGCAATGT AAGCTCTATG AACGGTTATC TCCGATCTTT TTGTGACGAA CGGAGTACCG ATGCCCCGCC 1600
GGATGATTTT GACGGATGCC GAACGGCAGA ATTTCCTTGC ATTGCCGACC GATGATGACA CCCTGATCCG GCATTGGAGC CTTGATGATG ATGATCGTCG 1700
TCTTATCGAA ACTCGACGCC ATGATGACAC TCGACTAGGA TTGGCGCTTC AGCTCTGTGC GCTTCGTTAT CCAGGCCGCC TAATCCAGCG CGGGGAAGTC 1800
ATCCCTTCTG TGGCGCTGTC GTTTCTGGCC GAACAACTTG GGATCGATCC GGAGGCGCTT TCCACTTTTG CACGGAGAGC GGCAACCCGC TATGAGCAGC 1900
TTGCCATTCT GCGGCAGCAC TATGGCTTCA CCGAATTGTC CCATCCTTTG CGCGCCGACC TGCTTGCTTT CGCGCGCGGC ATTGCGATCG CTGCGACGAA 2000
AGACAAATTT GTCGTTGCAA CACTGGCTGA CGAGATGCGG CGGCGGCGCA TCGTTATTCC CGGCATTACC GTGCTCGAAC GGCTGGCCGG GCAGGCTTGT 2100
ACCGAAGCCG AGGAGGCGCT GTATGCCGAC GTCGCTGGAA GGTTGGCGCC AGACCTTATC ATCCGAATGG AAGCTTTGCT CGGCGCGGGA CGACGCGCAA 2200
AACAGAGCGG CATATCCTGG TTGCGAGAAC CTCCTGGCAA AGCCGGAGTA TCGGCCATGC GGGGATTGAT CGATCGGCTT GAGGCCGTGC GACATGCCGG 2300
TATTCCCAAT GATGTGCTTC AGGCGATCCC GGCTCACCGT GTTCGTCGGA TGGCACAGGA AGGCCGTCGT CTGACTGCCC AGAACTTTGA GCAGATGCGA 2400
CCTGGCCGCC GTCACGCGAC ATTGGCAGCG TTTCTTCTGG AAATGGAAAT CGCACTCACC GACGCGGCGA TCGGCATGTT CGAGACGTTG ATCGGCAAGA 2500
GTTTTCGACA GGCAGAGGCA ACCCGTGACA AGCAACTCCT CGAAAGCGCA TCGTCTGCGA CATCCGCACT CGATTTCTTC GCGAACTTCG GCGAAGCCAT 2600
CACCGCCCAA CGCGAATCCG GGCTTTCCCT TGATGAGGCG GTGACCACCG TGACGAGCTG GGAAGAGCTC ATTCAGGCAA CTGCCTCGGC AAAAGCGGTC 2700
AAAAGGCCTC GAAGCGAGGA TGATCTGATC ACCTACCTGT CGGCTCAATA TGTGCGCATC CGGCGGTTTG TCACTCCATT TTTGGCATCG TTCACCTTCG 2800
AAGGAAACCG CCGAAACGCA CATTTCATCG AAGCTATCGT GCGGATGGAA ACTGCCTGGA GGAAAGGCTC CAGATCAGCA GAGCAGCCTT GGGTCCGCAA 2900
GGCCTTGTTG CTGGCGAACG CGCGATGGCG CAAAGAAATC GTTGGCGCCG ATGGCGCTAT CGACCGGAAG ATGCTGGAAC TTTTTCTCAT CGTCGAACTG 3000
AAGAACAGGA TTGCCGCCGG AGACATATGG ATCAGGGGTT CCCGCGCTTA CCGTGCACTA GACGAGCACA TGATCTCGCA ACAGACGTTT GCTGTCATCA 3100
AAGCCGAGGC CCGTATTCCG GTCGCGATTC CGATCGATGT CGAGACCTAT CTCACCCAGA AGGCCGAAGC GCTGGATCAA AAACTGCGTG AGGCGGCACG 3200
TCGCCTGGAA GCCGGCCGAG GCGATACCCG CATCGGCGCG AAGGGATTGC GGGTTCCAGC GGTCAGGACC ACGGAAACCG AAGCCGCAAC CGCATTTGCG 3300
AGACGTGTTG CAACCACCAT GCCGCCGGTC CGGTTGACGG ATCTTGTAGC CGATATTGAC CGGATGACCG GCTTCAGTTC GCTGTTCGAG CATCTTCAGA 3400
CCGGTCGGCC ACCTTCTAAC TTGCGCGTGT TCTATGCGGC GCTGATCGCC GAAGCCACAA ATCTCGGCTT CTCGAAGATG GCGCATGCCT GCCCCGGCAT 3500
CACACGCCGA CAGCTACAGC AGATGGCAAT CTGGCATTTC CGCGAAGAGA CCTTCACGCT AGCGCTCGCC AAGCTGGTGG AAGCTCAACA CTCCACGCCA 3600
TTCTCGACCA TGTTCGGATC GAACACAGTT TCCTCGTCGG ACGGACAGCA TATCCATCTT GGTGACGGTG GCGAAGTCGC TGGCAGCGTC AATGGCAGGT 3700
ATGGAAACAA CCCCATCATC AAACTCTACA CCGCGATCTC TGGCGGCTAC GCGCCCTTTC ACACAAAAAT CATCGCCGCG ACGGCGAGTG AAGCCGTTCA 3800
TGTGCTCGAC GCATTGCTGG AAACTGATGC CGGTCTCCAT ATTATCCGTC ATCATGTCGA CGGCGGCGGC GTCAGCGATC TTGTGTTTGC CCTCTGTCAT 3900
GCGCTCGGCT TCGCGTTCGT GCCGCGCATC CCCGATCTCG ACGGGCGATG CCTCTATGGC TTCGAACCGG CTAAGCAATA CGGAATTCTA CAGAATGTCA 4000
TGGGTGACCG GATCGACGCT GATCTGATCC GGGCAAATTG GGACGACATA TTGCGCCTGA TGACCTCGCT GCGCACACGC ACCGTCAGCG CTTCGCTCAT 4100
GCTGAAGCGG CTATCAGCAA CCACGAGGCA AAGTGGTCTC GCCCAGGCGT TGCGCCAGAT GGGCCGGATT GAACGAACCA TATTCACCCT CGACTGGATC 4200
AACGACGAGC AATTGCGCAA GACCACGACG GCCGAATTGA ACAAGGGAGA AAGCCGGAAC AGTCTCGTTC GTGCGGTCAA TCTTCATCGT CTGGGCCGTT 4300
TCCGCGATCG CAGCCAGGAA AACCTTTCGA TCCGGGCATC CGCACTCAAC CTGATCGTCA GCGCCATCAT TCATTGGAAC ACAATCTACA CCGGCCGCAT 4400
CGTTGATGCC CTGCGCAATG CTGGACAGGC TATTCCGGAC CATCTGCTGT CCAGCCTGTC ACCGCTCACA TGGGAGCATG TGAACCTGAC TGGCGATTAC 4500
CTCTGGGAAG AAAAACCTGC TCTTGATGAA AACGGGTTCC GCGCAATCCC GTTCGTCCTA TGATCCGCCT ACCGTATATC TGCGCCCCTT TAATGTTCAG 4600
GGGCC
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| Recombination Sites |
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| Name |
Coordinates |
Gene |
Sequence |
| res |
1524-1585 |
62 |
AACCCGTCTT GCAATGTAAG CTCTATGAAC GGTTATCTCC GATCTTTTTG TGACGAACGG
AG |
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| ORFs |
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| Gene Name |
Associated TE |
Coordinates |
Class |
Sub Class |
Orientation |
| aminoglycoside phosphotransferase |
Tn163 |
53-823 |
Passenger Gene |
Antibiotic Resistance |
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| tnpR |
Tn163 |
820-1461 |
Accessory Gene |
Resolvase |
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| tnpA |
Tn163 |
1591-4563 |
Transposase |
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| aminoglycoside phosphotransferase |
Aminoglycoside phosphotransferase |
Tn163 |
771 |
53-823 |
- |
| Class: | Passenger Gene |
| Sub Class: | Antibiotic Resistance |
| Target: | aminoglycoside antibiotic (ARO:0000016) |
| Sequence Family: | APH (Pfam:PF01636) |
| Comment: | no match in CARD |
Protein Sequence:
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MKELEGGRAG QIWRDGNTVR RPSGPWTPTV HRFLRHLQNS GFSAAPIPVE LTQHQETVTY VKGRVCEHLA DRLVGSERML RSAGELLRKF HSASRGFLDV
DREIQMWMLP PQEPREIICH GDFAPYNVAT EGDAAVGIID FDTAHPAPAL WDLAYAVYRW APLSDYSNHG VVFDVREQLH RASIFCASYG ATADEKRRLP
EAICGRLQAL VDFMHSRAET GDSTFVEDVE AGDAAIYLRD IEYVQKNRDR LLSALG
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnpR |
TnpR |
Tn163 |
642 |
820-1461 |
- |
| Class: | Accessory Gene |
| Sub Class: | Resolvase |
| Function: | resolvase |
| Transpoase Chemistry: | Serine |
| Sequence Family: | Serine Site-Specific Recombinase |
Protein Sequence:
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MTALIGYARV SKADGSQVHD LQRDALIKAG VEVDNVYEDS ASGKQDDRPG LAACLKLRAG DTLAVWKLDR LGRDLRHLVN LVDDLTKRNI GLKVLAGEGA
SIDTSTANGR LVFGIFAALA EFERALIIER TKAGLAAARA RGRNGGAPFK MTPAKLRLAQ AMMGKPETKV ADLCAELGIT RQTLYRFVGP KGELRGDGER
LLGRKKITLG VRK
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| tnpA |
TnpA |
Tn163 |
2973 |
1591-4563 |
+ |
| Class: | Transposase |
| Function: | transposase |
| Transpoase Chemistry: | DDE |
Protein Sequence:
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MPRRMILTDA ERQNFLALPT DDDTLIRHWS LDDDDRRLIE TRRHDDTRLG LALQLCALRY PGRLIQRGEV IPSVALSFLA EQLGIDPEAL STFARRAATR
YEQLAILRQH YGFTELSHPL RADLLAFARG IAIAATKDKF VVATLADEMR RRRIVIPGIT VLERLAGQAC TEAEEALYAD VAGRLAPDLI IRMEALLGAG
RRAKQSGISW LREPPGKAGV SAMRGLIDRL EAVRHAGIPN DVLQAIPAHR VRRMAQEGRR LTAQNFEQMR PGRRHATLAA FLLEMEIALT DAAIGMFETL
IGKSFRQAEA TRDKQLLESA SSATSALDFF ANFGEAITAQ RESGLSLDEA VTTVTSWEEL IQATASAKAV KRPRSEDDLI TYLSAQYVRI RRFVTPFLAS
FTFEGNRRNA HFIEAIVRME TAWRKGSRSA EQPWVRKALL LANARWRKEI VGADGAIDRK MLELFLIVEL KNRIAAGDIW IRGSRAYRAL DEHMISQQTF
AVIKAEARIP VAIPIDVETY LTQKAEALDQ KLREAARRLE AGRGDTRIGA KGLRVPAVRT TETEAATAFA RRVATTMPPV RLTDLVADID RMTGFSSLFE
HLQTGRPPSN LRVFYAALIA EATNLGFSKM AHACPGITRR QLQQMAIWHF REETFTLALA KLVEAQHSTP FSTMFGSNTV SSSDGQHIHL GDGGEVAGSV
NGRYGNNPII KLYTAISGGY APFHTKIIAA TASEAVHVLD ALLETDAGLH IIRHHVDGGG VSDLVFALCH ALGFAFVPRI PDLDGRCLYG FEPAKQYGIL
QNVMGDRIDA DLIRANWDDI LRLMTSLRTR TVSASLMLKR LSATTRQSGL AQALRQMGRI ERTIFTLDWI NDEQLRKTTT AELNKGESRN SLVRAVNLHR
LGRFRDRSQE NLSIRASALN LIVSAIIHWN TIYTGRIVDA LRNAGQAIPD HLLSSLSPLT WEHVNLTGDY LWEEKPALDE NGFRAIPFVL
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| References |
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Ulrich A, Pühler A. The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains. Mol Gen Genet. 1994 Mar;242(5):505-16. doi: 10.1007/BF00285274. PubMed ID: 8121409
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