Transposon
Name: Tn10
Family: compound        Group: IS10
Evidence of Transposition: no
 Host     

Host Organism:Shigella flexneri Molecular Source:plasmid R100
Place of Origin:U.S.A and U.K. Date of Isolation:1975

 Map     



 Terminal Inverted Repeats (IR)     

IRL (Length: 17 bp)CTGATGAATCCCCTAAT
IRL (Length: 17 bp)CTGATGAATCCCCTAAT

 Sequence     
DNA SequenceLength  9147 
--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
CTGATGAATC CCCTAATGAT TTTTATCAAA ATCATTAAGT TAAGGTAGAT ACACATCTTG TCATATGATC AAATGGTTTC GCCAAAAATC AATAATCAGA 100
CAACAAAATG TGCGAACTCG ATATTTTACA CGACTCTCTT TACCAATTCT GCCCCGAATT ACACTTAAAA CGACTCAACA GCTTAACGTT GGCTTGCCAC 200
GCCTTACTTG ACTGTAAAAC TCTCACTCTT ACCGAACTTG GCCGTAACCT GCCAACCAAA GCGAGAACAA AACATAACAT CAAACGAATC GACCGATTGT 300
TAGGTAATCG TCACCTCCAC AAAGAGCGAC TCGCTGTATA CCGTTGGCAT GCTAGCTTTA TCTGTTCGGG CAATACGATG CCCATTGTAC TTGTTGACTG 400
GTCTGATATC CGTGAGCAAA AACGGCTTAT GGTATTGCGA GCTTCAGTCG CACTACACGG TCGTTCTGTT ACTCTTTATG AGAAAGCGTT CCCGCTTTCA 500
GAGCAATGTT CAAAGAAAGC TCATGACCAA TTTCTAGCCG ACCTTGCGAG CATTCTACCG AGTAACACCA CACCGCTCAT TGTCAGTGAT GCTGGCTTTA 600
AAGTGCCATG GTATAAATCC GTTGAGAAGC TGGGTTGGTA CTGGTTAAGT CGAGTAAGAG GAAAAGTACA ATATGCAGAC CTAGGAGCGG AAAACTGGAA 700
ACCTATCAGC AACTTACATG ATATGTCATC TAGTCACTCA AAGACTTTAG GCTATAAGAG GCTGACTAAA AGCAATCCAA TCTCATGCCA AATTCTATTG 800
TATAAATCTC GCTCTAAAGG CCGAAAAAAT CAGCGCTCGA CACGGACTCA TTGTCACCAC CCGTCACCTA AAATCTACTC AGCGTCGGCA AAGGAGCCAT 900
GGATTCTAGC AACTAACTTA CCTGTTGAAA TTCGAACACC CAAACAACTT GTTAATATCT ATTCGAAGCG AATGCAGATT GAAGAAACCT TCCGAGACTT 1000
GAAAAGTCCT GCCTACGGAC TAGGCCTACG CCATAGCCGA ACGAGCAGCT CAGAGCGTTT TGATATCATG CTGCTAATCG CCCTGATGCT TCAACTAACA 1100
TGTTGGCTTG CGGGCGTTCA TGCTCAGAAA CAAGGTTGGG ACAAGCACTT CCAGGCTAAC ACAGTCAGAA ATCGAAACGT ACTCTCAACA GTTCGCTTAG 1200
GCATGGAAGT TTTGCGGCAT TCTGGCTACA CAATAACAAG GGAAGACTCA CTCGTGGCTG CAACCCTGCT TACTCAAAAT CTATTCACAC ATGGTTACGT 1300
TTTGGGGAAA TTATGAGGGG ATCTCTCAGT GCTCAGCATG TTTGAGTGCT TTACTCGCCG CGGGTTGAGA AATATGTAAA ACTTCAGCCG CACGCGTTAA 1400
GGAACCACAA GTCATTACAG CATAAAAGAT ATCGAGATGT CTTAATTTCA TTCCATGTAG CCTACTGATT ATTTATTTCT ACGGACTATT CTAACGAATA 1500
AAGTATAAAT AATCAGATAT ACTCGTCATA ATTCAAATTT TGGTTTAGTT GTCACGAGAA TTAATTCGTA AACGTGCAAG CTAAATAGAT TGACTAGGGG 1600
AATATACGGG GTAAGAAAGC GGCTCGGCAT ACTGCCCTAT AATAAATTGT TAGGAGAAGT ACGCCGAGTA GTGTGTAGCT ATTAGGTAAA GTATGTACCA 1700
ATTTCAATAA ATATTTTAAT TAAGATACTG TTTGAAATAT CAACAATAAA GGCACCGACC ATAGGAACGA CAAGGAAAGC TTTATGTGAT GGTCCAAATG 1800
CTTTTGTGAC TGTTTGCATA TTCGCAATTG CTGTTGGTGT TGCTCCCATA CCAAAGCCAC AGTGACCCGC GCTGATCACG ACAGCATCAT AATCTTTGCC 1900
CATCATTTTG AAGGTGACAA AGCAGGCAAA TAGCACCATG ACAACAGTTT GTACAGCAAT GATAATTAAT ACTGGCCCTG CCATGCTTGC CAATTGACCA 2000
AATTTTAATG ACATTAACGC CATTGCCAAG AAAAGCGATA AAGCAACGCT ACCTAATACA TCGACGGTCG GCTCAAACAC TTCGTGTTTA AATACATGAG 2100
TCAGTGTATT ACGGATAATA ATACCGACAA ATAAACACCA GACAAAAGTA GGCAGTTGCA GAAAAGTATC TTTAAACAAT GCACTGATAT AGCCACCAAC 2200
AACAATACAG ATAATCAGCA TTGAAATGGT TTCAATAACG TTATTTGCAT TGATTTTTCT TTTGACGCTT GGTTGCTCAA AAGCTTCAAC GATAGTGTCG 2300
CGCTCTTGCT CGGTTGTTTT AGGAATAGAG ACCTTTTTCA AAAGATGACG GGCAACAGGG CCGCCAACTA AACCACCCAA CACTAATCCA AGTGTTGCAC 2400
AAGCCATCGC TAATTCAACG GCGCCTGTTA CACCATATTT ATCAGCGAGA ATAGGGCCCC ATGCTCCGGC ATTACCATGA CCACCTGTTA GAGTAATTGA 2500
ACCTGCAATT AAGCCAATAA ATGGACTTTC ATTCATCATG ACAGCCATAC TCATGCCGAC AGTATTTTGA ATGGCGATTA GGATCGTTAC TGCAATAGTT 2600
AATAGAACTA ACGGCTTTCC TCCTTTAATC AGTCGAGAAA AGTCAGAACT TAGCCCGATA GAGGAAAAGA ATGTGAGCAT TAATAAACTT TGCAATGAAG 2700
CATCAAAAGT AAACGAATAA CCTGATGTTT TATCAATAAT TAACAGAACA ATTGCAACAA TAAAGCCACC AACGACGGCT TCTGGTATGT GGTTTTTTTG 2800
TAGGAACGGG GTAAATTTTA CGACAAACAT TCCTATGAGT AGCGCGATAC ATGCGACTAA TAATGTATAA CTGGCATCTA GGATCATTTT TTTACCTCTA 2900
TTAGTTTACC AGAATTTTCA CTATAACAAT TTAACAATAG TGAAAAATGA AGTTACTTTG GTTAACTAAT CTTTAACAAT GAGTTTTATT AGGGTATCCA 3000
TAACTTTTAG TTAATAGGGT CATAATTTTA GTGTGATCTT AATCATGAAA TATTAAAAAG ATTAAAATAA CAACAATGGT ATTTAATGAT GGGTAAACCT 3100
AAATACCATG CGGGATCCGC ACTATTTTCG GTGATAGCAA AAAATAAAAA AGTATAAATA ATAAACAAAA TTATAAGAGA AGGAGAACAT GATAAGCGCA 3200
GGCCGAGCAA TTTAAAGAAG TGTGATATAC AACATAAAAC AGCAGCATGA CCTTTCTATA TTTCGTTGCT TATCGAAGTG TTAATTTTCG GGTGGATTAC 3300
TCTGTTTGTT GATTAATAAC GGAGAAAAAT ATGATTGCTG TAATATTTGA GGTGCAAATA CAACCCGACC AACAAACTCG CTATTTGACT TTAGCTGAGG 3400
AGTTAAGACC ACTATTAAGT CATGTAGCTG GTTTTATTTC AATTGAACGT TTTCAAAGTC TAGCTACAGA AGGAAAAATG TTATCGCTAT CTTGGTGGGA 3500
AAACGAATAC GCAGTTCTGC AATGGAAAAA TCATGTTTTA CATGCGAAAG CTCAACAAGA AGGGCGAGAG TCAATATTTG ATTTTTACAA AATTAGTATT 3600
GCTCATATTA CTCGCGAATA TTCATTTAAA AAGGACAAGG ATAATGTTTG ATGTTCACGT TGTTTTAGAT AATCAAATAG GACAATTAGC ATTACTAGGA 3700
AAAACATTAG GTAATAAAGG TATTGGATTG GAAGGGGGAG GGATATTTAC GGTTGGTGAT GAATGCCATG CTCATTTTCT TGTTGAACAA GGAAAGGAAG 3800
CTAAAATAGC GCTAGAGCAA GCTGGACTGT TAGTACTTGC GATCCGGACA CCATTAATTC GTAAGTTAAA ACAGGAAAAA CCGGGGGAAC TTGGCGAAAT 3900
AGCACGAGTA TTGGCGGAGA ATAACATTAA TATTTTAGTG CAATACAGTG ACCATGCTAA CCAACTGATA TTAATAACGG ACAATGATAG TATGGCTGCA 4000
TCTGTTACGC TCCCTTGGGC AATAAAGTGA ACTTGCGATG GCTAATTTAA TACGAAAAGA GGTTACCTTT GAGTCCTCAA TAGCCGCGAT AGGGGCGGCT 4100
ATGTCTGACA TTTCACGAGT TAAAATACTC AGTGCTTTGA TGGATGGGCG AGCTTGGACG GCCACTGAGC TAAGTTCTGT GGCGAATATA TCAGCTTCAA 4200
CGGCGAGCAG TCATTTATCT AAATTATTAG ATTGCCAGCT AATCACAGTA GTAGCTCAAG GCAAGCATCG TTATTTTCGG CTAGCAGGAA AAGATATTGC 4300
TGAATTGATG GAAAGTATGA TGGGGATCTC CTTAAACCAT GGCGTACATG CCAAAGTTTC CACGCCAGTG CATTTACGAA AAGCACGTAC TTGCTATGAT 4400
CATTTAGCTG GCGAAGTTGC CGTTAAGATC TATGATTCCC TTTGTCAACA GCAATGGATC ACTGAAAATG GTTCAATGAT CACATTAAGT GGTATTCAAT 4500
ATTTTCATGA AATGGGAATT GACGTTCCTT CCAAACATTC ACGTAAAATC TGTTGTGCGT GTTTAGATTG GAGTGAACGC CGTTTCCATT TAGGTGGGTA 4600
CGTTGGAGCC GCATTATTTT CGCTTTATGA ATCTAAAGGG TGGTTAACTC GACATCTTGG TTACCGTGAA GTTACCATCA CGGAAAAAGG TTATGCTGCT 4700
TTTAAGACCC ACTTTCACAT TTAAGTTGTT TTTCTAATCC GCATATGATC AATTCAAGGC CGAATAAGAA GGCTGGCTCT GCACCTTGGT GATCAAATAA 4800
TTCGATAGCT TGTCGTAATA ATGGCGGCAT ACTATCAGTA GTAGGTGTTT CCCTTTCTTC TTTAGCGACT TGATGCTCTT GATCTTCCAA TACGCAACCT 4900
AAAGTAAAAT GCCCCACAGC GCTGAGTGCA TATAATGCAT TCTCTAGTGA AAAACCTTGT TGGCATAAAA AGGCTAATTG ATTTTCGAGA GTTTCATACT 5000
GTTTTTCTGT AGGCCGTGTA CCTAAATGTA CTTTTGCTCC ATCGCGATGA CTTAGTAAAG CACATCTAAA ACTTTTAGCG TTATTACGTA AAAAATCTTG 5100
CCAGCTTTCC CCTTCTAAAG GGCAAAAGTG AGTATGGTGC CTATCTAACA TCTCAATGGC TAAGGCGTCG AGCAAAGCCC GCTTATTTTT TACATGCCAA 5200
TACAATGTAG GCTGCTCTAC ACCTAGCTTC TGGGCGAGTT TACGGGTTGT TAAACCTTCG ATTCCGACCT CATTAAGCAG CTCTAATGCG CTGTTAATCA 5300
CTTTACTTTT ATCTAATCTA GACATCATTA ATTCCTAATT TTTGTTGACA CTCTATCATT GATAGAGTTA TTTTACCACT CCCTATCAGT GATAGAGAAA 5400
AGTGAAATGA ATAGTTCGAC AAAGATCGCA TTGGTAATTA CGTTACTCGA TGCCATGGGG ATTGGCCTTA TCATGCCAGT CTTGCCAACG TTATTACGTG 5500
AATTTATTGC TTCGGAAGAT ATCGCTAACC ACTTTGGCGT ATTGCTTGCA CTTTATGCGT TAATGCAGGT TATCTTTGCT CCTTGGCTTG GAAAAATGTC 5600
TGACCGATTT GGTCGGCGCC CAGTGCTGTT GTTGTCATTA ATAGGCGCAT CGCTGGATTA CTTATTGCTG GCTTTTTCAA GTGCGCTTTG GATGCTGTAT 5700
TTAGGCCGTT TGCTTTCAGG GATCACAGGA GCTACTGGGG CTGTCGCGGC ATCGGTCATT GCCGATACCA CCTCAGCTTC TCAACGCGTG AAGTGGTTCG 5800
GTTGGTTAGG GGCAAGTTTT GGGCTTGGTT TAATAGCGGG GCCTATTATT GGTGGTTTTG CAGGAGAGAT TTCACCGCAT AGTCCCTTTT TTATCGCTGC 5900
GTTGCTAAAT ATTGTCACTT TCCTTGTGGT TATGTTTTGG TTCCGTGAAA CCAAAAATAC ACGTGATAAT ACAGATACCG AAGTAGGGGT TGAGACGCAA 6000
TCGAATTCGG TATACATCAC TTTATTTAAA ACGATGCCCA TTTTGTTGAT TATTTATTTT TCAGCGCAAT TGATAGGCCA AATTCCCGCA ACGGTGTGGG 6100
TGCTATTTAC CGAAAATCGT TTTGGATGGA ATAGCATGAT GGTTGGCTTT TCATTAGCGG GTCTTGGTCT TTTACACTCA GTATTCCAAG CCTTTGTGGC 6200
AGGAAGAATA GCCACTAAAT GGGGCGAAAA AACGGCAGTA CTGCTCGGAT TTATTGCAGA TAGTAGTGCA TTTGCCTTTT TAGCGTTTAT ATCTGAAGGT 6300
TGGTTAGTTT TCCCTGTTTT AATTTTATTG GCTGGTGGTG GGATCGCTTT ACCTGCATTA CAGGGAGTGA TGTCTATCCA AACAAAGAGT CATCAGCAAG 6400
GTGCTTTACA GGGATTATTG GTGAGCCTTA CCAATGCAAC CGGTGTTATT GGCCCATTAC TGTTTGCTGT TATTTATAAT CATTCACTAC CAATTTGGGA 6500
TGGCTGGATT TGGATTATTG GTTTAGCGTT TTACTGTATT ATTATCCTGC TATCGATGAC CTTCATGTTA ACCCCTCAAG CTCAGGGGAG TAAACAGGAG 6600
ACAAGTGCTT AGTTATTTCG TCACCAAATG ATGTTATTCC GCGAAATATA ATGACCCTCT TGATAACCCA AGAGGGCATT TTTTACGATA AAGAAGATTT 6700
AGCTTCAAAT AAAACCTATC TATTTTATTT ATCTTTCAAG CTCAATAAAA AGCCGCGGTA AATAGCAATA AATTGGCCTT TTTTATCGGC AAGCTCTTTT 6800
AGGTTTTTCG CATGTATTGC GATATGCATA AACCAGCCAT TGAGTAAGTT TTTAAGCACA TCATCATCAT AAGCTTTAAG TTGGTTCTCT TGGATCAATT 6900
TGCTGACAAT GGCGTTTACC TTACCAGTAA TGTATTCAAG GCTAATTTTT TCAAGTTCAT TCCAACCAAT GATAGGCATC ACTTCTTGGA TAGGGATAAG 7000
GTTTTTATTA TTATCAATAA TATAATCAAG ATAATGTTCA AATATACTTT CTAAGGCAGA CCAACCATTT GTTAAATCAG TTTTTGTTGT GATGTAGGCA 7100
TCAATCATAA TTAATTGCTG CTTATAACAG GCACTGAGTA ATTGTTTTTT ATTTTTAAAG TGATGATAAA AGGCACCTTT GGTCACCAAC GCTTTTCCCG 7200
AGATCTCATC TATTGAAACA GCTTGATAGC CTTTTTCAAC AAACAATATT CGTGCTGAGT TAACCAGTGA TTGATAGGTA CTCTTAAAAT TTTCTTGTTG 7300
ATGATTTTTA TTTTCCATGA TAGATTTAAA ATAACATACC GTCAGTATGT TTATGGTATC ATGATGATGT GGTCGTGACA ATCTTAAGAA CATTTAGGTT 7400
ATTTTATGTA TATTGAACAG CATTCTCGCT ATCAAAATAA AGCTAATAAC ATCCAATTAA GATATGATGA TAAGCAGTTT CATACAACGG TTATCAAAGA 7500
TGTTCTATTA TGGATTGAAC ATAATTTAGA TCAGTCTTTA CTGCTTGATG ATGTGGCGAA TAAAGCGGGT TATACCAAGT GGTATTTTCA GCGGCTGTTC 7600
AAAAAAGTAA CAGGGGTCAC ACTGGCTAGC TATATTCGTG CTCGTCGTTT GACGAAAGCG GCTGTTGAGT TGAGGTTGAC GAAAAAAACT ATCCTTGAGA 7700
TCGCATTAAA ATATCAATTT GATTCCCAAC AATCTTTTAC ACGTCGATTT AAGTACATTT TTAAGGTTAC ACCAAGTTAT TATCGGCGTA ATAAATTATG 7800
GGAATTGGAG GCAATGCACT GAGAGATCCC CTCATAATTT CCCCAAAGCG TAACCATGTG TGAATAAATT TTGAGCTAGT AGGGTTGCAG CCACGAGTAA 7900
GTCTTCCCTT GTTATTGTGT AGCCAGAATG CCGCAAAACT TCCATGCCTA AGCGAACTGT TGAGAGTACG TTTCGATTTC TGACTGTGTT AGCCTGGAAG 8000
TGCTTGTCCC AACCTTGTTT CTGAGCATGA ACGCCCGCAA GCCAACATGT TAGTTGAAGC ATCAGGGCGA TTAGCAGCAT GATATCAAAA CGCTCTGAGC 8100
TGCTCGTTCG GCTATGGCGT AGGCCTAGTC CGTAGGCAGG ACTTTTCAAG TCTCGGAAGG TTTCTTCAAT CTGCATTCGC TTCGAATAGA TATTAACAAG 8200
TTGTTTGGGT GTTCGAATTT CAACAGGTAA GTTAGTTGCT AGAACCCATG GCTCCTTTGC CGACGCTGAG TAGATTTTAG GTGACGGGTG GTGACAATGA 8300
GTCCGTGTCG AGCGCTGATT TTTTCGGCCT TTAGAGCGAG ATTTATACAA TAGAATTTGG CATGAGATTG GATTGCTTTT AGTCAGCCTC TTATAGCCTA 8400
AAGTCTTTGA GTGACTAGAT GACATATCAT GTAAGTTGCT GATAGGTTTC CAGTTTTCCG CTCCTAGGTC TGCATATTGT ACTTTTCCTC TTACTCGACT 8500
TAACCAGTAC CAACCCAGCT TCTCAACGGA TTTATACCAT GGCACTTTAA AGCCAGCATC ACTGACAATG AGCGGTGTGG TGTTACTCGG TAGAATGCTC 8600
GCAAGGTCGG CTAGAAATTG GTCATGAGCT TTCTTTGAAC ATTGCTCTGA AAGCGGGAAC GCTTTCTCAT AAAGAGTAAC AGAACGACCG TGTAGTGCGA 8700
CTGAAGCTCG CAATACCATA AGTCGTTTTT GCTCACGAAT ATCAGACCAG TCAACAAGTA CAATGGGCAT CGTATTGCCC GAACAGATAA AGCTAGCATG 8800
CCAACGGTAT ACAGCGAGTC GCTCTTTGTG GAGGTGACGA TTACCTAACA ATCGGTCGAT TCGTTTGATG TTATGTTTTG TTCTCGCTTT GGTTGGCAGG 8900
TTACGGCCAA GTTCGGTAAG AGTGAGAGTT TTACAGTCAA GTAATGCGTG GCAAGCCAAC GTTAAGCTGT TGAGTCGTTT TAAGTGTAAT TCGGGGCAGA 9000
ATTGGTAAAG AGAGTCGTGT AAAATATCGA GTTCGCACAT CTTGTTGTCT GATTATTGAT TTTTCGCGAA ACCATTTGAT CATATGACAA GATGTGTATC 9100
CACCTTAACT TAATGATTTT TACCAAAATC ATTAGGGGAT TCATCAG

 ORFs     
ORF Summary
Gene Name Associated TE Coordinates Class Sub Class Orientation
tnp IS10L 108-1316 Transposase   +
jemA Tn10 1682-2887 Passenger Gene Other -
jemB Tn10 3331-3651 Passenger Gene Other +
CPT07_26605 Tn10 3644-4030 Passenger Gene Hypothetical +
jemC Tn10 4038-4724 Passenger Gene Other +
tetR (ARO:3003479) Tn10 4702-5328 Passenger Gene Antibiotic Resistance -
tet(B) (ARO:3000166) Tn10 5407-6612 Passenger Gene Antibiotic Resistance +
tetC Tn10 6725-7318 Passenger Gene Antibiotic Resistance -
tetD Tn10 7406-7822 Passenger Gene Antibiotic Resistance +
tnp IS10R 7832-9040 Transposase   -

ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp Tnp IS10L 1209 108-1316 +
Class:   Transposase
Function:   transposase
Transpoase Chemistry:   DDE
Protein Sequence:  
MCELDILHDS LYQFCPELHL KRLNSLTLAC HALLDCKTLT LTELGRNLPT KARTKHNIKR IDRLLGNRHL HKERLAVYRW HASFICSGNT MPIVLVDWSD
IREQKRLMVL RASVALHGRS VTLYEKAFPL SEQCSKKAHD QFLADLASIL PSNTTPLIVS DAGFKVPWYK SVEKLGWYWL SRVRGKVQYA DLGAENWKPI
SNLHDMSSSH SKTLGYKRLT KSNPISCQIL LYKSRSKGRK NQRSTRTHCH HPSPKIYSAS AKEPWILATN LPVEIRTPKQ LVNIYSKRMQ IEETFRDLKS
PAYGLGLRHS RTSSSERFDI MLLIALMLQL TCWLAGVHAQ KQGWDKHFQA NTVRNRNVLS TVRLGMEVLR HSGYTITRED SLVAATLLTQ NLFTHGYVLG
KL

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
jemA JemA Tn10 1206 1682-2887 -
Class:   Passenger Gene
Sub Class:   Other
Function:   glutamate:sodium symporter activity (GO:0015501)
Comment:   sodium-dependent glutamate permease
Protein Sequence:  
MILDASYTLL VACIALLIGM FVVKFTPFLQ KNHIPEAVVG GFIVAIVLLI IDKTSGYSFT FDASLQSLLM LTFFSSIGLS SDFSRLIKGG KPLVLLTIAV
TILIAIQNTV GMSMAVMMNE SPFIGLIAGS ITLTGGHGNA GAWGPILADK YGVTGAVELA MACATLGLVL GGLVGGPVAR HLLKKVSIPK TTEQERDTIV
EAFEQPSVKR KINANNVIET ISMLIICIVV GGYISALFKD TFLQLPTFVW CLFVGIIIRN TLTHVFKHEV FEPTVDVLGS VALSLFLAMA LMSLKFGQLA
SMAGPVLIII AVQTVVMVLF ACFVTFKMMG KDYDAVVISA GHCGFGMGAT PTAIANMQTV TKAFGPSHKA FLVVPMVGAF IVDISNSILI KIFIEIGTYF
T

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
jemB JemB Tn10 321 3331-3651 +
Class:   Passenger Gene
Sub Class:   Other
Sequence Family:  Antibiotic biosynthesis monooxygenase (Pfam:PF03992)
Protein Sequence:  
MIAVIFEVQI QPDQQTRYLT LAEELRPLLS HVAGFISIER FQSLATEGKM LSLSWWENEY AVLQWKNHVL HAKAQQEGRE SIFDFYKISI AHITREYSFK
KDKDNV

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
CPT07_26605 CPT07_26605 Tn10 387 3644-4030 +
Class:   Passenger Gene
Sub Class:   Hypothetical
Comment:   Amino acid-binding protein
Protein Sequence:  
MFDVHVVLDN QIGQLALLGK TLGNKGIGLE GGGIFTVGDE CHAHFLVEQG KEAKIALEQA GLLVLAIRTP LIRKLKQEKP GELGEIARVL AENNINILVQ
YSDHANQLIL ITDNDSMAAS VTLPWAIK

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
jemC JemC Tn10 687 4038-4724 +
Class:   Passenger Gene
Sub Class:   Other
Sequence Family:  HTH ArsR-type DNA-binding domain (InterPro:IPR001845)
Comment:   N-terminal 100 amino acids similar to bacterial transcriptional repressors and metal-binding proteins (PMID:10781570)
Protein Sequence:  
MANLIRKEVT FESSIAAIGA AMSDISRVKI LSALMDGRAW TATELSSVAN ISASTASSHL SKLLDCQLIT VVAQGKHRYF RLAGKDIAEL MESMMGISLN
HGVHAKVSTP VHLRKARTCY DHLAGEVAVK IYDSLCQQQW ITENGSMITL SGIQYFHEMG IDVPSKHSRK ICCACLDWSE RRFHLGGYVG AALFSLYESK
GWLTRHLGYR EVTITEKGYA AFKTHFHI

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tetR (ARO:3003479) TetR Tn10 627 4702-5328 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic target alteration (ARO:0001001); antibiotic efflux (ARO:0010000)
Transpoase Chemistry:   repressor of the tetracycline resistance element
Target:   tetracycline antibiotic (ARO:3000050)
Sequence Family:  major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002)
Comment:   perfect match to reference sequence for ARO:3003479
Protein Sequence:  
MMSRLDKSKV INSALELLNE VGIEGLTTRK LAQKLGVEQP TLYWHVKNKR ALLDALAIEM LDRHHTHFCP LEGESWQDFL RNNAKSFRCA LLSHRDGAKV
HLGTRPTEKQ YETLENQLAF LCQQGFSLEN ALYALSAVGH FTLGCVLEDQ EHQVAKEERE TPTTDSMPPL LRQAIELFDH QGAEPAFLFG LELIICGLEK
QLKCESGS

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tet(B) (ARO:3000166) Tet(B) Tn10 1206 5407-6612 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   antibiotic efflux (ARO:0010000)
Transpoase Chemistry:   antibiotic efflux (ARO:0010000)
Target:   tetracycline antibiotic (ARO:3000050)
Sequence Family:  major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002)
Comment:   strict match to reference sequence for ARO:3000166 (bitscore:786)||Synonym: tetB
Protein Sequence:  
MNSSTKIALV ITLLDAMGIG LIMPVLPTLL REFIASEDIA NHFGVLLALY ALMQVIFAPW LGKMSDRFGR RPVLLLSLIG ASLDYLLLAF SSALWMLYLG
RLLSGITGAT GAVAASVIAD TTSASQRVKW FGWLGASFGL GLIAGPIIGG FAGEISPHSP FFIAALLNIV TFLVVMFWFR ETKNTRDNTD TEVGVETQSN
SVYITLFKTM PILLIIYFSA QLIGQIPATV WVLFTENRFG WNSMMVGFSL AGLGLLHSVF QAFVAGRIAT KWGEKTAVLL GFIADSSAFA FLAFISEGWL
VFPVLILLAG GGIALPALQG VMSIQTKSHQ QGALQGLLVS LTNATGVIGP LLFAVIYNHS LPIWDGWIWI IGLAFYCIII LLSMTFMLTP QAQGSKQETS
A

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tetC TetC Tn10 594 6725-7318 -
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Function:   DNA binding (GO:0003677)
Target:   tetracycline antibiotic (ARO:3000050)
Sequence Family:  Bacterial regulatory proteins, tetR family (Pfam:PF00440)
Comment:   no match in CARD
Protein Sequence:  
MENKNHQQEN FKSTYQSLVN SARILFVEKG YQAVSIDEIS GKALVTKGAF YHHFKNKKQL LSACYKQQLI MIDAYITTKT DLTNGWSALE SIFEHYLDYI
IDNNKNLIPI QEVMPIIGWN ELEKISLEYI TGKVNAIVSK LIQENQLKAY DDDVLKNLLN GWFMHIAIHA KNLKELADKK GQFIAIYRGF LLSLKDK

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tetD TetD Tn10 417 7406-7822 +
Class:   Passenger Gene
Sub Class:   Antibiotic Resistance
Target:   tetracycline antibiotic (ARO:3000050)
Comment:   no match in CARD
Protein Sequence:  
MYIEQHSRYQ NKANNIQLRY DDKQFHTTVI KDVLLWIEHN LDQSLLLDDV ANKAGYTKWY FQRLFKKVTG VTLASYIRAR RLTKAAVELR LTKKTILEIA
LKYQFDSQQS FTRRFKYIFK VTPSYYRRNK LWELEAMH

Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp Tnp IS10R 1209 7832-9040 -
Class:   Transposase
Function:   transposase
Transpoase Chemistry:   DDE
Protein Sequence:  
MCELDILHDS LYQFCPELHL KRLNSLTLAC HALLDCKTLT LTELGRNLPT KARTKHNIKR IDRLLGNRHL HKERLAVYRW HASFICSGNT MPIVLVDWSD
IREQKRLMVL RASVALHGRS VTLYEKAFPL SEQCSKKAHD QFLADLASIL PSNTTPLIVS DAGFKVPWYK SVEKLGWYWL SRVRGKVQYA DLGAENWKPI
SNLHDMSSSH SKTLGYKRLT KSNPISCQIL LYKSRSKGRK NQRSTRTHCH HPSPKIYSAS AKEPWVLATN LPVEIRTPKQ LVNIYSKRMQ IEETFRDLKS
PAYGLGLRHS RTSSSERFDI MLLIALMLQL TCWLAGVHAQ KQGWDKHFQA NTVRNRNVLS TVRLGMEVLR HSGYTITRED LLVAATLLAQ NLFTHGYALG
KL

 Internal Transposable Elements (TE)     

TnCentral Accession TE Name Type Coordinates Length
IS10L-AF162223 IS10L Insertion Sequence 1-1329 1329
IS10R-AF162223 IS10R Insertion Sequence 7819-9147 1329

 Internal Repeat Elements     

Name Associated Mobile Element Coordinates Sequence (Top Strand)
IRR IS10L 1308-1329 TTTAATACTC CCCTAGAGAG TC
IRR IS10R 7819-7840 CTGAGAGATC CCCTCATAAT TT

 References     

1.Foster TJ, Howe TG, Richmond KM. Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K-12 chromosome. J Bacteriol. 1975 Dec;124(3):1153-8. doi: 10.1128/JB.124.3.1153-1158.1975. PubMed ID: 1104574
2.Kleckner N, Chan RK, Tye BK, Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975 Oct 5;97(4):561-75. doi: 10.1016/s0022-2836(75)80059-3. PubMed ID: 1102715