TN Report

Transposon

Name: In2

Family: Tn402 Group: Class 1

Evidence of Transposition: no

Host

Host Organism:	Shigella flexneri	Molecular Source:	plasmid NR1 (R100)
Place of Origin:	Japan	Date of Isolation:	1950s

Map

Terminal Inverted Repeats (IR)

Sequence

DNA Sequence

Length 11000

--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100

TGTCATTTTC AGAAGACGAC TGCACCAGTT GATTGGGCGT AATGGCTGTT GTGCAGCCAG CTCCTGACAG TTCAATATCA GAAGTGATCT GCACCAATCT 100
CGACTATGCT CAATACTCGT GTGCACCAAA GCGAGGTGAG CATGGCGACG GACACCCCAC GGATTCCAGA ACAAGGCGTG GCCACTCTGC CTGATGAGGC 200
TTGGGAGCGT GCGCGCCGTC GTGCGGAGAT CATCAGTCCG TTGGCGCAGT CGGAGACGGT CGGGCACGAA GCGGCCGATA TGGCGGCTCA GGCGCTGGGC 300
TTGTCTCGGC GCCAGGTATA CGTTCTGATC CGGCGTGCCC GGCAAGGCAG CGGCCTCGTG ACGGATCTGG TGCCCGGCCA GTCCGGTGGA GGTAAAGGTA 400
AGGGGCGCTT GCCGGAACCG GTCGAGCGCG TCATCCACGA GCTACTGCAA AAGCGGTTCC TGACCAAGCA GAAGCGCAGC CTAGCGGCCT TTCACCGCGA 500
AGTCACTCAG GTGTGCAAGG CTCAAAAACT GCGAGTGCCG GCGCGCAATA CCGTGGCCTT ACGGATCGCT AGCCTTGACC CGCGCAAGGT CATCCGCCGG 600
CGGGAAGGCC AGGATGCCGC TCGTGACCTA CAAGGTGTGG GCGGCGAGCC TCCTGCCGTG ACCGCGCCGC TGGAGCAGGT GCAGATAGAC CATACGGTCA 700
TCGACCTGAT CGTGGTCGAT GACCGCGACC GGCAACCTAT TGGCCGCCCG TACCTGACCC TCGCCATCGA CGTGTTCACC CGCTGCGTGC TCGGCATGGT 800
CGTCACGCTG GAAGCGCCGT CTGCCGTTTC GGTTGGCCTG TGCCTCGTGC ATGTCGCCTG CGACAAGCGC CCTTGGCTGG AAGGACTGAA CGTGGAAATG 900
GATTGGCAGA TGAGCGGCAA GCCCTTGCTG CTCTACCTAG ACAACGCGGC CGAGTTCAAG AGCGAGGCCC TGCGCCGGGG TTGCGAGCAG CATGGCATCC 1000
GGCTGGACTA TCGCCCGCTG GGACAGCCGC ACTATGGCGG CATCGTGGAA CGGATCATCG GCACGGCGAT GCAGATGATT CACGACGAAC TGCCGGGAAC 1100
GACCTTCTCC AACCCTGACC AGCGCGGCGA CTACGATTCC GAAAACAAGG CCGCCCTGAC GCTGCGCGAG CTAGAGCGCT GGCTCACATT GGCGGTCGGC 1200
ACCTACCACG GTTCGGTGCA CAACGGCCTG CTCCAACCGC CGGCCGCGCG CTGGGCCGAG GCCGTGGCGC GTGTCGGCGT ACCGGCCGTC GTCACACGCG 1300
CTACTTCGTT CCTGGTCGAT TTTCTGCCGA TCCTCCGGCG CACGCTGACC CGCACCGGCT TTGTCATCGA CCACATCCAC TACTACGCCG ATGCGCTCAA 1400
GCCGTGGATT GCGCGGCGTG AACGCTGGCC GTCCTTTCTG ATCCGGCGCG ATCCGCGCGA CATCAGCCGT ATCTGGGTCC TGGAACCGGA GGGACAGCAT 1500
TACCTGGAAA TTCCCTACCG TACCTTGTCG CATCCGGCTG TCACCCTCTG GGAACAACGG CAGGCGCTGG CGAAACTGCG GCAGCAAGGG CGCGAACAGG 1600
TGGATGAGTC GGCGCTGTTC CGCATGATCG GCCAGATGCG TGAGATTGTG ACCAGCGCGC AGAAGGCCAC ACGCAAGGCG CGGCGTGACG CGGATCGCCG 1700
CCAGCACCTC AAGACATCAG CTCGGCCGGA CAAGCCCGTT CCGCCGGATA CGGATATTGC CGACCCGCAG GCAGACAACT TGCCACCCGC CAAACCGTTC 1800
GACCAGATTG AGGAGTGGTA GCCGTGGACG AATATCCCAT CATCGACCTG TCCCACCTGC TGCCGGCGGC CCAGGGCTTG GCCCGTCTTC CGGCGGACGA 1900
GCGCATCCAG CGCCTTCGCG CCGACCGCTG GATCGGCTAT CCGCGCGCAG TCGAGGCGCT GAACCGGCTG GAAGCCCTTT ATGCGTGGCC AAACAAGCAA 2000
CGCATGCCCA ACCTGCTGCT GGTTGGCCCG ACCAACAATG GCAAGTCGAT GATCGTCGAG AAGTTCCGCC GCACCCACCC GGCCAGCTCC GACGCCGACC 2100
AGGAGCACAT CCCGGTGTTG GTCGTGCAGA TGCCGTCCGA GCCGTCCGTG ATCCGCTTCT ACGTCGCGCT GCTCGCCGCG ATGGGCGCGC CGCTGCGCCC 2200
ACGCCCACGG TTGCCGGAAA TGGAGCAACT GGCTCTGGCA CTGCTGCGCA AGGTCGGCGT GCGCATGCTG GTGATCGACG AGCTGCACAA CGTGCTGGCC 2300
GGCAACAGCG TCAACCGCCG GGAATTCCTC AACCTGCTGC GCTTCCTCGG CAACGAACTG CGCATCCCGT TGGTTGGGGT AGGCACGCGC GACGCCTACC 2400
TAGCCATCCG CTCCGATGAC CAGTTGGAAA ATCGCTTCGA GCCGATGATG CTGCCGGTAT GGGAGGCCAA CGACGATTGC TGCTCACTGC TGGCCAGCTT 2500
CGCCGCTTCG CTCCCGCTGC GCCGGCCTTC CCCAATTGCC ACGCTGGACA TGGCTCGCTA CCTGCTCACA CGCAGCGAGG GCACCATAGG GGAACTGGCG 2600
CACTTGCTGA TGGCGGCGGC CATCGTCGCC GTGGAGAGCG GCGAGGAAGC GATCAACCAT CGCACACTCA GCATGGCCTG TTGAGTTGCA TCTAAAATTG 2700
ACCCACTGGG GGTGCGGACG ATTTCTTGGA CGGTTTATAC GGACATCAAT CCGACCGCAT GACGATACTC GATGGGACTA CGCCCGCCAA GCGACACTTT 2800
GATGCGGCGC TCGTTGTACC AGTGGATATA GGCATCGATT CGCGTCATGA GGTCTTTCAG CGTCACGTGC TGCCAATTCC TCGGGTAGAT TAGTTCGGTC 2900
TTCAATCGTC CGAAAAAGCC CTCGCATGCA GCATTGTCTG GCGAGCAGCC CTTTTTGGAC ATCGACCGCG TTAATTGGGC ATTTTCAGTG CGGCGGATCC 3000
ACGCAGGCCA GCGATAATGC GAGCCCCTGT CCGAATGGAT AACCGGATGC TCACCGGGTC GCAGTGTCCG TACCGCGTGA TCCAGCATGG TATTGACCAG 3100
GTTCGCATCC GGGCTGGTGC CGATATTCCA GGCCACCACC AGCCCATCGA AGCAATCGAC GATCGGCGAG ACGTAGACCT TCCCTGCCGG AATGTGTATT 3200
TCCGTCAGAT CGGTCAACCA TTTCGTATTC GGCGCCGACG CGTGAAAGTC GCGATTCAGC AGATTCGGGA CCGCTGGTGT CGGGTCGCCA GCATACGCCG 3300
AGAAGCGCCG GCGGCGCGGT GTTCTCACGA CCAGACGCTC TTGCGCCATC AAGCGACGCA CGACCTTCTC GGACACACGC ATGCCACCAA GGCGCAAGGC 3400
ACTATCAATG CGTCGATAGC CATAGCAGCG GTAGTTGTCC TCGAAGATAG TCCGAATGAC CTCACGCACC TGCGTGTACT TGTCGGGCCG CGTCTGCCGC 3500
AGGCGTTGAT AGAAGTATGT GCTGCGCGCC AGCTTCAGGC CGCACAACAG ATTGGCTAAT GGAAACGTGA CTCTGAGGGC ATCAACCACC TTCGTTTTTT 3600
CTCGGCTTGT CAGTTCGAGG GGGTTGATGC CCATGTCTTT TTTTATCAAT TCACTCGCCT TCTCCAGAAT TGCATTCTCC ATGCGAAGCC GCTGGTTCTG 3700
GCTCTCCAGT TCGGCCAGTT CCCTGAGTAG TGCCTCATGC CGCTGCTCGA GCGAGGTGTC ACCTTTCTTC TTTGTCATGG GTTTTAGGGG CACTTTGCCA 3800
AGTAATCGAT GCTGCCAGTT ATACAACGTT GGTCGCGATA CACCGACAGT GTCGGCCACA TCCTTTGCCG AACCTACGCG CAGGTTCAGT GCAATGACGG 3900
CTTGCTGCTT CTCGAGGCGA GAGCGGGCGA CTGTGGGAGC GCTGCTGCCG ACGACCGTCC TAGCGAATTC AGGGCGTAAA TCACGGATCC AGGCACGCAA 4000
GGCCTCGCGG CTTGGGTAGC CCAGGCTTCG GATTGTGTGA CTCAGGCAGT AGCCTTGTTC GATATAGTGA TCTACTGCCC GTTGCTTTTG CTCATCGGTG 4100
TACTGCCGTT TTATCCGTTG ATAGCCTCGG CGAAGATCCT GATTCCGTTC GAATTCTGCC AACCAGGCCT TCAGCGAGTT CTTGGTGGGG TATCCCAGCT 4200
GCCGTAGTGT GGCGCTCATC CGGCGCCCAA GCTTCAGGTA CAACCTCACG GCTCGAAGGC GATCTTCATA CGAATACATG AACTACTCCT AAAGTAGTCC 4300
AAGATTTTGT CCGCACCCCA ACTTAGGGTA AAGATTTGCG TCGAAATTTG ACCCACGTAT GACACTGTTT CCCGTCTGGA TATGGCGGGA GAAATCAAGG 4400
AGTGATAAAC GTGGCGATAT TGAGCGCAAT TCGACGCTGG CATTTTCGCG ATGGTGCGTC GATTCGGGAA ATAGCCCGAC GAAGCGGCCT GTCCAGGAAC 4500
ACCGTTCGCA AGTATTTGCA AAGCAAGGTG GTTGAACCGC AGTACCCAGC GCGAGACAGC GTTGGCAAGT TAAGTCCTTT TGAGCCCAAG TTAAGGCAGT 4600
GGCTCTCCAC CGAGCACAAA AAGACAAAGA AGCTGCGCAG AAACCTGCGC AGCATGTACC GGGATTTGGT CGCTTTGGGC TTTACCGGGT CTTATGACCG 4700
AGTGTGTGCC TTTGCCCGAC AGTGGAAAGA TTCCGAACAG TTCAAGGCGC AAACCTCGGG CAAGGGTTGT TTCATCCCCT TGCGCTTTGC TTGTGGCGAA 4800
GCCTTCCAAT TCGATTGGAG TGAGGACTTT GCCCGCATAG CGGGCAAACA GGTCAAACTT CAGATTGCCC AGTTTAAGTT GGCCCACAGC CGGGCCTTTG 4900
TGCTTCGGGC TTACTACCAG CAAAAACATG AAATGCTGTT TGATGCCCAC TGGCATGCCT TTCAAATCTT CGGTGGCATT CCCAAGCGCG GCATCTACGA 5000
CAACATGAAG ACCGCTGTGG ATTCGGTGGG GCGTGGCAAA GAGCGCAGGG TCAATCAGCG GTTCACTGCC ATGGTCAGCC ACTACCTGTT TGATGCGCAG 5100
TTCTGTAATC CAGCATCGGG TTGGGAGAAA GGCCAGATTG AGAAGAACGT GCAGGATTCC CGCCAACGCC TGTGGCAAGG GGCACCAGAC TTTCAAAGCC 5200
TTGCTGATTT GAATGTGTGG CTTGAGCATC GCTGCAAAGC GCTGTGGTCT GAGCTGCGCC ACCCCGAATT GGACCAAACC GTGCAAGAGG CCTTTGCCGA 5300
TGAACAAGGC GAGTTGATGG CGCTACCCAA TGCCTTTGAT GCATTCGTGG AGCAAACCAA GCGAGTCACT TCAACCTGCC TTGTTCACCA CGAGGGCAAT 5400
CGCTACAGCG TTCCTGCCAG TTACGCCAAC AGGGCCATCA GCCTTCGGAT TTATGCAGAC AAGCTGGTGA TGGCTGCCGA AGGCCAACAC ATTGCCGAGC 5500
ATCCAAGATT GTTTGGCAGT GGCCACGCTC GGCGTGGCCA CACACAATAC GACTGGCACC ATTACTTGTC TGTGCTTCAG AAGAAACCTG GGGCGTTGCG 5600
CAATGGTGCG CCATTTGCTG AATTGCCACC CGCGTTCAAG AAGCTTCAAT CCATCTTGCT GCAACGCCCC GGCGGTGACC GTGACATGGT GGAAATTCTG 5700
GCCCTTGTAT TGCACCACGA TGAAGGTGCG GTACTCAGTG CTGTGGAATT GGCATTGGAG TGTGGCAAGC CATCGAAGGA GCATGTGCTT AATCTGTTGG 5800
GACGTTTGAC CGAAGAACCT CCACCCAAAC CGATTCCAAT TCCCAAGGGG TTAAGGCTGA CATTGGAACC ACAGGCCAAC GTGAACCGCT ATGACAGTTT 5900
AAGGAGAGCC CATGATGCAG CATGAAGGCC ATGTGAGAAT CCTCAAATCC TTGAAACTCT TTGGCATGGC ACACGCCATT GAGGAGTTGG GCAATCAGAA 6000
TTCACCAGCA TTTAATCAAG CCTTGCCCAT GCTGGACAGC TTGATTAAAG CTGAAGTGGC AGAGCGTGAA GTACGTTCGG TGAACTATCA ATTGCGGGTG 6100
GCCAAGTTCC CCGTGTATCG GGACTTGGTG GGCTTTGACT TCAGTCAAAG CCTGGTTAAT GAGGCCACGG TCAAACAATT GCACCGGTGC GACTTCATGG 6200
AACAAGCCCA GAACGTGGTG CTGATTGGTG GGCCAGGCAC AGGCAAGACT CACCTGGCCA CAGCCATTGG TACACAAGCA GTGATGCACT TGAACCGACG 6300
GGTGCGTTTC TTCTCCACCG TGGATTTGGT CAATGCACTG GAGCAAGAGA AATCATCTGG GCGTCAGGGA CAAATCGCAA ACCGTCTGTT GTATGCCGAT 6400
TTGGTGATTC TGGATGAGCT GGGATATTTG CCTTTTAGCC AAACCGGTGG GGCACTGCTG TTTCACCTGC TCTCAAAGCT GTACGAAAAA ACCAGCGTGA 6500
TACTGACCAC CAACTTGAGC TTCTCGGAAT GGAGCCGAGT GTTTGGCGAT GAAAAGATGA CAACAGCGTT GTTGGACCGA CTAACCCACC ACTGCCACAT 6600
CCTGGAAACC GGCAATGAAA GTTACCGCTT CAAACACAGT TCAACTCAGA ATAAGCAGGA GGAAAAACAG ACCCGCAAAC TGAAAATCGA GACATAATTC 6700
TGACAACAAG GGGTGGGTCA AAATTCAATG CAAATCCCGG GTCAAATTTG GGTGCAAATC AACAGATATC GACAACCTCT CGCGCAACCA AGACATCGCG 6800
GTCGGACTGC AAGTGATCTT GAAGCCACGG GCCCGTCCCA CCCCGACATG GACCTCGATG CCCGAACGGA CGTTAGATTT CGAGTTCTAG GCGTTCTGCG 6900
ATGAAGGTTG GATCCCAGCC GGGATTGAAA GTGTCGACGT GGGTGAATCC GAGCCGCTCG TATAGGCCAC GCAGGTTCGG GTGGCAGTCG AGCCGCAGCT 7000
TGGCGCACCC CTGCGTTCGC GCGGCATGGC GGCAAGCCTC GATCAGCGCG GAGCTGACAC CCCGGCCCGC ATGTGTCCGT CGCACCGCGA GCTTGTGCAG 7100
ATATGCGGCC TCCCCCTTGA GGGCGTCGGG CCAGAACTCG GGATCCTCGG CCGACAAGGT GCAACAGCCG ACGATGCCGT CGCTGCAACT CGCGACTAGG 7200
AGCTCGGATC TCAGGACGAA GGTCTCCGCG AATGTCCGGT CGATCCGCGC GACGTCCCAG GCGGGCGTTC CCTTGGCGGA CATCCACGCC GCAGCGTCGT 7300
GCATCAGCCG CACAACCTCG TCGATATCAC CCGAGCAGGC GACCCGAACG TTCGGAGGCT CCTCGCTGTC CATTCGCTCC CCTGGCGCGG TATGAACCGC 7400
CGCCTCATAG TGCAGTTTGA TCCTGACGAG CCCAGCATGT CTGCGCCCAC CTTCGCGGAA CCTGACCAGG GTCCGCTAGC GGGCGGCCGG AAGGTGAATG 7500
CTAGGCATGA TCTAACCCTC GGTCTCTGGC GTCGCGACTG CGAAATTTCG CGAGGGTTTC CGAGAAGGTG ATTGCGCTTC GCAGATCTCC AGGCGCGTGG 7600
GTGCGGACGT AGTCAGCGCC ATTGCCGATC GCGTGAAGTT CCGCCGCAAG GCTCGCTGGA CCCAGATCCT TTACAGGAAG GCCAACGGTG GCGCCCAAGA 7700
AGGATTTCCG CGACACCGAG ACCAATAGCG GAAGCCCCAA CGCCGACTTC AGCTTTTGAA GGTTCGACAG CACGTGCAGC GATGTTTCCG GTGCGGGGCT 7800
CAAGAAAAAT CCCATCCCCG GATCGAGGAT GAGCCGGTCG GCAGCGACCC CGCTCCGTCG CAAGGCGGAA ACCCGCGCCT CGAAGAACCG CACAATCTCG 7900
TCGAGCGCGT CTTCGGGTCG AAGGTGACCG GTGCGGGTGG CGATGCCATC CCGCTGCGCT GAGTGCATAA CCACCAGCCT GCAGTCCGCC TCAGCAATAT 8000
CGGGATAGAG CGCAGGGTCA GGAAATCCTT GGATATCGTT CAGGTAGCCC ACGCCGCGCT TGAGCGCATA GCGCTGGGTT TCCGGTTGGA AGCTGTCGAT 8100
TGAAACACGG TGCATCTGAT CGGACAGGGC GTCTAAGAGC GGCGCAATAC GTCTGATCTC ATCGGCCGGC GATACAGGCC TCGCGTCCGG ATGGCTGGCG 8200
GCCGGTCCGA CATCCACGAC GTCTGATCCG ACTCGCAGCA TTTCGATCGC CGCGGTGACA GCGCCGGCGG GGTCTAGCCG CCGGCTCTCA TCGAAGAAGG 8300
AGTCCTCGGT GAGATTCAGA ATGCCGAACA CCGTCACCAT GGCGTCGGCC TCCGCAGCGA CTTCCACGAT GGGGATCGGG CGAGCAAAAA GGCAGCAATT 8400
ATGAGCCCCA TACCTACAAA GCCCCACGCA TCAAGCTTTT GCCCATGAAG CAACCAGGCA ATGGCTGTAA TTATGACGAC GCCGAGTCCC GACCAGACTG 8500
CATAAGCAAC ACCGACAGGG ATGGATTTCA GAACCAGAGA AAGAAAATAA AATGCGATGC CATAACCGAT TATGACAACG GCGGAAGGGG CAAGCTTAGT 8600
AAAGCCCTCG CTAGATTTTA ATGCGGATGT TGCGATTACT TCGCCAACTA TTGCGATAAC AAGAAAAAGC CAGCCTTTCA TGATATATCT CCCAATTTGT 8700
GTAGGGCTTA TTATGCACGC TTAAAAATAA TAAAAGCAGA CTTGACCTGA TAGTTTGGCT GTGAGCAATT ATGTGCTTAG TGCATCTAAC GCTTGAGTTA 8800
AGCCGCGCCG CGAAGCGGCG TCGGCTTGAA CGAATTGTTA GACATTATTT GCCGACTACC TTGGTGATCT CGCCTTTCAC GTAGTGGACA AATTCTTCCA 8900
ACTGATCTGC GCGCGAGGCC AAGCGATCTT CTTCTTGTCC AAGATAAGCC TGTCTAGCTT CAAGTATGAC GGGCTGATAC TGGGCCGGCA GGCGCTCCAT 9000
TGCCCAGTCG GCAGCGACAT CCTTCGGCGC GATTTTGCCG GTTACTGCGC TGTACCAAAT GCGGGACAAC GTAAGCACTA CATTTCGCTC ATCGCCAGCC 9100
CAGTCGGGCG GCGAGTTCCA TAGCGTTAAG GTTTCATTTA GCGCCTCAAA TAGATCCTGT TCAGGAACCG GATCAAAGAG TTCCTCCGCC GCTGGACCTA 9200
CCAAGGCAAC GCTATGTTCT CTTGCTTTTG TCAGCAAGAT AGCCAGATCA ATGTCGATCG TGGCTGGCTC GAAGATACCT GCAAGAATGT CATTGCGCTG 9300
CCATTCTCCA AATTGCAGTT CGCGCTTAGC TGGATAACGC CACGGAATGA TGTCGTCGTG CACAACAATG GTGACTTCTA CAGCGCGGAG AATCTCGCTC 9400
TCTCCAGGGG AAGCCGAAGT TTCCAAAAGG TCGTTGATCA AAGCTCGCCG CGTTGTTTCA TCAAGCCTTA CGGTCACCGT AACCAGCAAA TCAATATCAC 9500
TGTGTGGCTT CAGGCCGCCA TCCACTGCGG AGCCGTACAA ATGTACGGCC AGCAACGTCG GTTCGAGATG GCGCTCGATG ACGCCAACTA CCTCTGATAG 9600
TTGAGTCGAT ACTTCGGCGA TCACCGCTTC CCTCATGATG TTTAACTTTG TTTTAGGGCG ACTGCCCTGC TGCGTAACAT CGTTGCTGCT CCATAACATC 9700
AAACATCGAC CCACGGCGTA ACGCGCTTGC TGCTTGGATG CCCGAGGCAT AGACTGTACC CCAAAAAAAC AGTCATAACA AGCCATGAAA ACCGCCACTG 9800
CGCCGTTACC ACCGCTGCGT TCGGTCAAGG TTCTGGACCA GTTGCGTGAG CGCATACGCT ACTTGCATTA CAGCTTACGA ACCGAACAGG CTTATGTCCA 9900
CTGGGTTCGT GCCTTCATCC GTTTCCACGG TGTGCGTCAC CCGGCAACCT TGGGCAGCAG CGAAGTCGAG GCATTTCTGT CCTGGCTGGC GAACGAGCGC 10000
AAGGTTTCGG TCTCCACGCA TCGTCAGGCA TTGGCGGCCT TGCTGTTCTT CTACGGCAAG GTGCTGTGCA CGGATCTGCC CTGGCTTCAG GAGATCGGAA 10100
GACCTCGGCC GTCGCGGCGC TTGCCGGTGG TGCTGACCCC GGATGAAGTG GTTCGCATCC TCGGTTTTCT GGAAGGCGAG CATCGTTTGT TCGCCCAGCT 10200
TCTGTATGGA ACGGGCATGC GGATCAGTGA GGGTTTGCAA CTGCGGGTCA AGGATCTGGA TTTCGATCAC GGCACGATCA TCGTGCGGGA GGGCAAGGGC 10300
TCCAAGGATC GGGCCTTGAT GTTACCCGAG AGCTTGGCAC CCAGCCTGCG CGAGCAGCTG TCGCGTGCAC GGGCATGGTG GCTGAAGGAC CAGGCCGAGG 10400
GCCGCAGCGG CGTTGCGCTT CCCGACGCCC TTGAGCGGAA GTATCCGCGC GCCGGGCATT CCTGGCCGTG GTTCTGGGTT TTTGCGCAGC ACACGCATTC 10500
GACCGATCCA CGGAGCGGTG TCGTGCGTCG CCATCACATG TATGACCAGA CCTTTCAGCG CGCCTTCAAA CGTGCCGTAG AACAAGCAGG CATCACGAAG 10600
CCCGCCACAC CGCACACCCT CCGCCACTCG TTCGCGACGG CCTTGCTCCG CAGCGGTTAC GACATTCGAA CCGTGCAGGA TCTGCTCGGC CATTCCGACG 10700
TCTCTACGAC GATGATTTAC ACGCATGTGC TGAAAGTTGG CGGTGCCGGA GTGCGCTCAC CGCTTGATGC GCTGCCGCCC CTCACTAGTG AGAGGTAGGG 10800
CAGCGCAAGT CAATCCTGGC GGATTCACTA CCCCTGCGCG AAGGCCATCG GTGCCGCATC GAACGGCCGG TTGCGGAAAG TCCTCCCTGC GTCCGCTGAT 10900
GGCCGGCAGC AGCCCGTCGT TGCCTGATGG ATCCAACCCC TCCGCTGCTA TAGTGCAGTC GGCTTCTGAC GTTCAGTGCA GCCGTCTTCT GAAAACGACA 11000

Recombination Sites

Name	Coordinates	Gene	Sequence
attC qacH_sul1 core	7473-7506	34	CCGCTAGCGG GCGGCCGGAA GGTGAATGCT AGGC
attC aadA1a core	8790-8843	54	CGCTTGAGTT AAGCCGCGCC GCGAAGCGGC GTCGGCTTGA ACGAATTGTT AGAC
attI	9646-9701	56	CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCA

ORFs

ORF Summary

Gene Name	Associated TE	Coordinates	Class	Sub Class	Orientation
tniA	In2	142-1821	Transposase		+
tniB delta1	In2	1824-2684	Accessory Gene		+
tnp	IS1353	2735-4279	Transposase		-
istA	IS1326::IS1353	4402-5925	Transposase		+
istB	IS1326::IS1353	5912-6697	Accessory Gene	ATPase Transposition Helper	+
GNAT_fam	In2	6873-7373	Passenger Gene	Antibiotic Resistance	-
sul1 (ARO:3000410)	In2	7501-8340	Passenger Gene	Antibiotic Resistance	-
qacEdelta1 (ARO:3005010)	In2	8334-8681	Passenger Gene	Antibiotic Resistance	-
aadA (ARO:3002601)	In2	8845-9636	Passenger Gene	Antibiotic Resistance	-
intI1	In2	9785-10798	Integron Integrase	Class 1	+

ORF Details

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
tniA	TniA	In2	1680	142-1821	+

Class:	Transposase
Transpoase Chemistry:	DDE
Comment:	can be extended upstream by 12 amino acids\| identical to tniA (Tn1721 and In2)\| 25% amino acid sequence identity to TnsB from Tn7
Protein Sequence:	MATDTPRIPE QGVATLPDEA WERARRRAEI ISPLAQSETV GHEAADMAAQ ALGLSRRQVY VLIRRARQGS GLVTDLVPGQ SGGGKGKGRL PEPVERVIHE LLQKRFLTKQ KRSLAAFHRE VTQVCKAQKL RVPARNTVAL RIASLDPRKV IRRREGQDAA RDLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDDRDRQPI GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLVHVAC DKRPWLEGLN VEMDWQMSGK PLLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAVARVGV PAVVTRATSF LVDFLPILRR TLTRTGFVID HIHYYADALK PWIARRERWP SFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR EIVTSAQKAT RKARRDADRR QHLKTSARPD KPVPPDTDIA DPQADNLPPA KPFDQIEEW

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
tniB delta1	TniB delta1	In2	861	1824-2684	+

Class:	Accessory Gene
Function:	probable ATP-binding protein.
Comment:	probably truncated by insertion of IS1326::IS1353
Protein Sequence:	MDEYPIIDLS HLLPAAQGLA RLPADERIQR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLMAAAIVAV ESGEEAINHR TLSMAC

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
tnp	Tnp	IS1353	1545	2735-4279	-

Class:	Transposase
Function:	transposase
Transpoase Chemistry:	DDE
Protein Sequence:	MYSYEDRLRA VRLYLKLGRR MSATLRQLGY PTKNSLKAWL AEFERNQDLR RGYQRIKRQY TDEQKQRAVD HYIEQGYCLS HTIRSLGYPS REALRAWIRD LRPEFARTVV GSSAPTVARS RLEKQQAVIA LNLRVGSAKD VADTVGVSRP TLYNWQHRLL GKVPLKPMTK KKGDTSLEQR HEALLRELAE LESQNQRLRM ENAILEKASE LIKKDMGINP LELTSREKTK VVDALRVTFP LANLLCGLKL ARSTYFYQRL RQTRPDKYTQ VREVIRTIFE DNYRCYGYRR IDSALRLGGM RVSEKVVRRL MAQERLVVRT PRRRRFSAYA GDPTPAVPNL LNRDFHASAP NTKWLTDLTE IHIPAGKVYV SPIVDCFDGL VVAWNIGTSP DANLVNTMLD HAVRTLRPGE HPVIHSDRGS HYRWPAWIRR TENAQLTRSM SKKGCSPDNA ACEGFFGRLK TELIYPRNWQ HVTLKDLMTR IDAYIHWYNE RRIKVSLGGR SPIEYRHAVG LMSV

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
istA	IstA	IS1326::IS1353	1524	4402-5925	+

Class:	Transposase
Transpoase Chemistry:	DDE
Protein Sequence:	MINVAILSAI RRWHFRDGAS IREIARRSGL SRNTVRKYLQ SKVVEPQYPA RDSVGKLSPF EPKLRQWLST EHKKTKKLRR NLRSMYRDLV ALGFTGSYDR VCAFARQWKD SEQFKAQTSG KGCFIPLRFA CGEAFQFDWS EDFARIAGKQ VKLQIAQFKL AHSRAFVLRA YYQQKHEMLF DAHWHAFQIF GGIPKRGIYD NMKTAVDSVG RGKERRVNQR FTAMVSHYLF DAQFCNPASG WEKGQIEKNV QDSRQRLWQG APDFQSLADL NVWLEHRCKA LWSELRHPEL DQTVQEAFAD EQGELMALPN AFDAFVEQTK RVTSTCLVHH EGNRYSVPAS YANRAISLRI YADKLVMAAE GQHIAEHPRL FGSGHARRGH TQYDWHHYLS VLQKKPGALR NGAPFAELPP AFKKLQSILL QRPGGDRDMV EILALVLHHD EGAVLSAVEL ALECGKPSKE HVLNLLGRLT EEPPPKPIPI PKGLRLTLEP QANVNRYDSL RRAHDAA

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
istB	IstB	IS1326::IS1353	786	5912-6697	+

Class:	Accessory Gene
Sub Class:	ATPase Transposition Helper
Function:	stimulates transposition
Protein Sequence:	MMQHEGHVRI LKSLKLFGMA HAIEELGNQN SPAFNQALPM LDSLIKAEVA EREVRSVNYQ LRVAKFPVYR DLVGFDFSQS LVNEATVKQL HRCDFMEQAQ NVVLIGGPGT GKTHLATAIG TQAVMHLNRR VRFFSTVDLV NALEQEKSSG RQGQIANRLL YADLVILDEL GYLPFSQTGG ALLFHLLSKL YEKTSVILTT NLSFSEWSRV FGDEKMTTAL LDRLTHHCHI LETGNESYRF KHSSTQNKQE EKQTRKLKIE T

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
GNAT_fam	GNAT_fam	In2	501	6873-7373	-

Class:	Passenger Gene
Sub Class:	Antibiotic Resistance
Target:	aminoglycoside antibiotic (ARO:0000016)
Sequence Family:	GNAT
Protein Sequence:	MDSEEPPNVR VACSGDIDEV VRLMHDAAAW MSAKGTPAWD VARIDRTFAE TFVLRSELLV ASCSDGIVGC CTLSAEDPEF WPDALKGEAA YLHKLAVRRT HAGRGVSSAL IEACRHAART QGCAKLRLDC HPNLRGLYER LGFTHVDTFN PGWDPTFIAE RLELEI

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
sul1 (ARO:3000410)	Sul1	In2	840	7501-8340	-

Class:	Passenger Gene
Sub Class:	Antibiotic Resistance
Function:	antibiotic target replacement (ARO:0001002)
Transpoase Chemistry:	dihydropteroate synthase
Target:	sulfonamide antibiotic (ARO:3000282)\|\|sulfone antibiotic (ARO:3003401)
Sequence Family:	sulfonamide resistant sul (ARO:3004238)
Comment:	perfect match to reference sequence for ARO:3000410
Protein Sequence:	MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP GDLRSAITFS ETLAKFRSRD ARDRGLDHA

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
qacEdelta1 (ARO:3005010)	QacEdelta1	In2	348	8334-8681	-

Class:	Passenger Gene
Sub Class:	Antibiotic Resistance
Function:	antibiotic efflux (ARO:0010000)
Target:	disinfecting agents and antiseptics (ARO:3005386)
Sequence Family:	major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002)
Comment:	subunit of the qac multidrug efflux pump\|\|perfect match to reference sequence for ARO:3005010 (bitscore:219)
Protein Sequence:	MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL ARSPSWKSLR RPTPW

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
aadA (ARO:3002601)	AadA	In2	792	8845-9636	-

Class:	Passenger Gene
Sub Class:	Antibiotic Resistance
Function:	antibiotic inactivation (ARO:0001004)
Transpoase Chemistry:	aminoglycoside nucleotidyltransferase
Target:	aminoglycoside antibiotic (ARO:0000016)
Sequence Family:	ANT(3'') (ARO:3004275)
Comment:	perfect match to reference sequence for ARO:3002601\|\|Synonyms: aadA1-pm, aadA, aadA1, aad(3'')(9)
Protein Sequence:	MREAVIAEVS TQLSEVVGVI ERHLEPTLLA VHLYGSAVDG GLKPHSDIDL LVTVTVRLDE TTRRALINDL LETSASPGES EILRAVEVTI VVHDDIIPWR YPAKRELQFG EWQRNDILAG IFEPATIDID LAILLTKARE HSVALVGPAA EELFDPVPEQ DLFEALNETL TLWNSPPDWA GDERNVVLTL SRIWYSAVTG KIAPKDVAAD WAMERLPAQY QPVILEARQA YLGQEEDRLA SRADQLEEFV HYVKGEITKV VGK

Gene Name	Protein Name	Associated TE	Gene Length	Coordinates	Strand
intI1	IntI1	In2	1014	9785-10798	+

Class:	Integron Integrase
Sub Class:	Class 1
Transpoase Chemistry:	Tyrosine
Sequence Family:	Class 1 Integron Tyrosine Integrase
Protein Sequence:	MKTATAPLPP LRSVKVLDQL RERIRYLHYS LRTEQAYVHW VRAFIRFHGV RHPATLGSSE VEAFLSWLAN ERKVSVSTHR QALAALLFFY GKVLCTDLPW LQEIGRPRPS RRLPVVLTPD EVVRILGFLE GEHRLFAQLL YGTGMRISEG LQLRVKDLDF DHGTIIVREG KGSKDRALML PESLAPSLRE QLSRARAWWL KDQAEGRSGV ALPDALERKY PRAGHSWPWF WVFAQHTHST DPRSGVVRRH HMYDQTFQRA FKRAVEQAGI TKPATPHTLR HSFATALLRS GYDIRTVQDL LGHSDVSTTM IYTHVLKVGG AGVRSPLDAL PPLTSER

Internal Transposable Elements (TE)

TnCentral Accession	TE Name	Type	Coordinates	Length
IS1326_IS1353-AF071413	IS1326	Insertion Sequence	2679-6764	4086
IS1353-AF071413	IS1353	Insertion Sequence	2707-4320	1614

Internal Repeat Elements

Name	Associated Mobile Element	Coordinates	Sequence (Top Strand)
repeat t1	In2	9-27	TCAGAAGACG ACTGCACCA
repeat t2	In2	49-67	AACACGTCGG TCGAGGACT
repeat t3	In2	78-97	TCAGAAGTGA TCTGCACCAA
repeat t4	In2	110-128	TCAATACTCG TGTGCACCA
IRL	IS1326::IS1353	2679-2704	TGTTGAGTTG CATCTAAAAT TGACCC
IRR	IS1353	2707-2719	TGGGGGTGCG GAC
IRL	IS1353	4309-4320	CAGGCGTGGG GT
IRR	IS1326::IS1353	6739-6764	CCCAGTTTAA ACCCACGTTT AGTTGT
repeat i4	In2	10881-10899	AGGAGGGACG CAGGCGACT
repeat i3	In2	10909-10927	CGTCGGGCAG CAACGGACT
repeat i2	In2	10951-10969	ATCACGTCAG CCGAAGACT
IRi	In2	10968-11000	CTGCAAGTCA CGTCGGCAGA AGACTTTTGC TGT

References

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