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Recombination Sites | |
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Internal Transposable Elements | |
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Internal Repeats | |
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| Name: IS1133 |
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| Family: IS3 Group: IS3 |
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| Evidence of Transposition: ND |
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| Map |
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| Terminal Inverted Repeats (IR) |
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| IRL (Length: 27 bp) |  | TGGACTTGCCTCGGTTTCGTTCCGGTC |
| IRR (Length: 27 bp) | | TGGACTTGCACCGGTTGTGTTCCGGTC |
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| Sequence |
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--------10 --------20 --------30 --------40 --------50 --------60 --------70 --------80 --------90 -------100
TGGACTTGCC TCGGTTTCGT TCCGGTCTTT TTGAGAGCAT TATTTGTTAT CAAGGAGACC ATATATGTCA TTAAAGCATA GTGATGAATT TAAGCGTGAT 100
GCAGTTCGCA TAGCACTCAC TAGTGGCTTA ACACGCCGTC AAGTTGCGTC AGATTTAAGT ATTGGGCTTT CCACGCTTGG GAAATGGATC GCATCAATTT 200
CCGATGAAAC TAAAATTCCT ACCCAAGACA CTGATCTTCT GCGTGAGAAT GAACGTTTAC GCAAAGAGAA CCGTATCCTT CGGGAGGAGA GGGAGATATT 300
AAAAAAGGCA GCAATATTTT TCGCAGTACA AAAGCTGTGA GATTTCAGTT TATTACGGAT TACCGTGGCT CTCTCTCACG TTCACGCATA TGTCGTTTGA 400
TGGGCGTAAC AGATCGTGGT TTACGTGCAT GGAAACGCCG TCCTCCATCA CTGCGCCAGC GTCGTGATCT TATACTTCTA GCGCATATAC GTGAGCAGCA 500
TCGGTTGTGT TTGGGGAGCT ATGGTAGGCC GCGTATGACA GAAGAGTTGA AAGCGCTGGG CCTGCAGGTT GGGCAGCGTC GGGTTGGACG TTTGATGCGC 600
CAGAATAACA TTACAGTTGT TCGAACGCGT AAATTCAAAC GGACAACGGA TAGTCATCAT ACCTTCAACA TTGCACCGAA CCTATTAAAA CAAGACTTTA 700
GCGCAAGCGC ACCCAACCAG AAATGGGCAG GCGATATCAC TTATGTTTGG ACCAGAGAAG GATGGGTCTA TCTTGCTGTT ATCCTTGACC TGTATTCCCG 800
TCGCGTGATT GGCTGGGCAA CAGGTGATCG ATTAAAGCAG GATCTTGCAT TAAGGGCACT GAATATGGCG TTGGCTTTAC GCAAACCACC ACCGGGTTGT 900
ATTCAACACA CAGACCGTGG GAGCCAATAT TGCGCTCATG AATATCAAAA GCTACTGCTC AAACATCAAT TGCTGCCGTC CATGAGCGGG AAAGGCAATT 1000
GTTTTGATAA CTCCGCAGTA GAAAGCTTCT TTAAATCATT AAAGGCTGAG TTGATTTGGC GCAGACACTG GCAAACAAGG CGAGATATTG AGATTGCAAT 1100
CTTCGAATAT ATAAATGGCT TTTATAATCC ACGCCGAAGA CATTCAACAC TCGGCTGGAA ATCGCCGGTG GCATTTGAGA AAAAAGCCGC TTAAAATGAG 1200
AGATAGACCG GAACACAACC GGTGCAAGTC CA
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| ORFs |
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| Gene Name |
Associated TE |
Coordinates |
Class |
Sub Class |
Orientation |
| orfAB |
IS1133 |
65-1194 |
Transposase |
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+ |
| orfA |
IS1133 |
65-340 |
Accessory Gene |
Regulator |
+ |
| orfB |
IS1133 |
337-1194 |
Transposase |
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| orfAB |
OrfAB |
IS1133 |
1130 |
65-1194 |
+ |
| Class: | Transposase |
| Transpoase Chemistry: | DDE |
| Comment: | fusion protein from -1 programmed frameshifting between orfA and orfB |
Protein Sequence:
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MSLKHSDEFK RDAVRIALTS GLTRRQVASD LSIGLSTLGK WIASISDETK IPTQDTDLLR ENERLRKENR ILREEREILK KAAIFFAVQK L*DFSLLRIT
VALSHVHAYV V*WA*QIVVY VHGNAVLHHC ASVVILYF*R IYVSSIGCVW GAMVGRV*QK S*KRWACRLG SVGLDV*CAR ITLQLFERVN SNGQRIVIIP
STLHRTY*NK TLAQAHPTRN GQAISLMFGP EKDGSILLLS LTCIPVA*LA GQQVID*SRI LH*GH*IWRW LYANHHRVVF NTQTVGANIA LMNIKSYCSN
INCCRP*AGK AIVLITPQ*K ASLNH*RLS* FGADTGKQGE ILRLQSSNI* MAFIIHAEDI QHSAGNRRWH LRKKPL
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| orfA |
OrfA |
IS1133 |
276 |
65-340 |
+ |
| Class: | Accessory Gene |
| Sub Class: | Regulator |
| Function: | transposase activity (GO:0004803) |
| Comment: | orfA, together with orfB, encodes a fusion protein formed by translational frame-shifting that is the transposase for IS1133 |
Protein Sequence:
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MSLKHSDEFK RDAVRIALTS GLTRRQVASD LSIGLSTLGK WIASISDETK IPTQDTDLLR ENERLRKENR ILREEREILK KAAIFFAVQK L
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| Gene Name |
Protein Name |
Associated TE |
Gene Length |
Coordinates |
Strand |
| orfB |
OrfB |
IS1133 |
858 |
337-1194 |
+ |
| Class: | Transposase |
| Function: | transposase activity (GO:0004803) |
| Transpoase Chemistry: | DDE |
| Comment: | orfB, together with orfA, encodes a fusion protein formed by translational frame-shifting that is the transposase for IS1133 |
Protein Sequence:
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MRFQFITDYR GSLSRSRICR LMGVTDRGLR AWKRRPPSLR QRRDLILLAH IREQHRLCLG SYGRPRMTEE LKALGLQVGQ RRVGRLMRQN NITVVRTRKF
KRTTDSHHTF NIAPNLLKQD FSASAPNQKW AGDITYVWTR EGWVYLAVIL DLYSRRVIGW ATGDRLKQDL ALRALNMALA LRKPPPGCIQ HTDRGSQYCA
HEYQKLLLKH QLLPSMSGKG NCFDNSAVES FFKSLKAELI WRRHWQTRRD IEIAIFEYIN GFYNPRRRHS TLGWKSPVAF EKKAA
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| References |
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| 1. | Siguier P, Varani A, Perochon J, Chandler M. Exploring bacterial insertion sequences with ISfinder: objectives, uses, and future developments. Methods Mol Biol. 2012;859:91-103. doi: 10.1007/978-1-61779-603-6_5. PubMed ID: 22367867
| | | 2. | Heinzel P, Werbitzky O, Distler J, Piepersberg W. A second streptomycin resistance gene from Streptomyces griseus codes for streptomycin-3"-phosphotransferase. Relationships between antibiotic and protein kinases. Arch Microbiol. 1988;150(2):184-92. doi: 10.1007/BF00425160. PubMed ID: 2844130
| | | 3. | Blot M, Hauer B, Monnet G. The Tn5 bleomycin resistance gene confers improved survival and growth advantage on Escherichia coli. Mol Gen Genet. 1994 Mar;242(5):595-601. doi: 10.1007/BF00285283. PubMed ID: 7510018
| | | 4. | Yuasa K, Sugiyama M. Bleomycin-induced beta-lactamase overexpression in Escherichia coli carrying a bleomycin-resistance gene from Streptomyces verticillus and its application to screen bleomycin analogues. FEMS Microbiol Lett. 1995 Oct 1;132(1-2):61-6. doi: 10.1111/j.1574-6968.1995.tb07811.x. PubMed ID: 7590166
| | | 5. | Reznikoff WS. The Tn5 transposon. Annu Rev Microbiol. 1993;47:945-63. doi: 10.1146/annurev.mi.47.100193.004501. PubMed ID: 7504907
| | | 6. | Steiniger-White M, Rayment I, Reznikoff WS. Structure/function insights into Tn5 transposition. Curr Opin Struct Biol. 2004 Feb;14(1):50-7. doi: 10.1016/j.sbi.2004.01.008. PubMed ID: 15102449
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