https://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&feed=atom&action=historyTransposons families/Tn402 family - Revision history2024-03-28T14:28:27ZRevision history for this page on the wikiMediaWiki 1.34.0https://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=3077&oldid=prevTnCentral at 12:56, 5 December 20222022-12-05T12:56:49Z<p></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 12:56, 5 December 2022</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l238" >Line 238:</td>
<td colspan="2" class="diff-lineno">Line 238:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br/></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><hr></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><hr></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">{{TnPedia}}</ins></div></td></tr>
</table>TnCentralhttps://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2995&oldid=prevTnCentral at 13:50, 4 December 20222022-12-04T13:50:20Z<p></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 13:50, 4 December 2022</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l238" >Line 238:</td>
<td colspan="2" class="diff-lineno">Line 238:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br/></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><hr></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><hr></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del style="font-weight: bold; text-decoration: none;">{{TnPedia}}</del></div></td><td colspan="2"> </td></tr>
</table>TnCentralhttps://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2648&oldid=prevTnCentral at 23:01, 20 March 20222022-03-20T23:01:33Z<p></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 23:01, 20 March 2022</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l235" >Line 235:</td>
<td colspan="2" class="diff-lineno">Line 235:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Bibliography==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==Bibliography==</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><<del class="diffchange diffchange-inline">references </del>/></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">{{Reflist|32em}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><<ins class="diffchange diffchange-inline">br</ins>/></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline"><hr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">{{TnPedia}}</ins></div></td></tr>
</table>TnCentralhttps://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2309&oldid=prevTnCentral at 22:54, 13 August 20212021-08-13T22:54:13Z<p></p>
<a href="https://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2309&oldid=2308">Show changes</a>TnCentralhttps://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2308&oldid=prevTnCentral at 22:47, 13 August 20212021-08-13T22:47:51Z<p></p>
<a href="https://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2308&oldid=2307">Show changes</a>TnCentralhttps://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2307&oldid=prevTnCentral at 22:15, 13 August 20212021-08-13T22:15:11Z<p></p>
<a href="https://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2307&oldid=2306">Show changes</a>TnCentralhttps://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2306&oldid=prevTnCentral at 21:47, 13 August 20212021-08-13T21:47:40Z<p></p>
<a href="https://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=2306&oldid=1994">Show changes</a>TnCentralhttps://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=1994&oldid=prevTnCentral at 15:28, 17 March 20212021-03-17T15:28:33Z<p></p>
<a href="https://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=1994&oldid=1993">Show changes</a>TnCentralhttps://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=1993&oldid=prevTnCentral at 15:25, 17 March 20212021-03-17T15:25:28Z<p></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 15:25, 17 March 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l93" >Line 93:</td>
<td colspan="2" class="diff-lineno">Line 93:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>A phylogenetic tree of the concatenated TniA,B,Q and R proteins ([[:File:Fig-Tn402.8.png|Fig. Tn402.8]]) indicated that Tn''5053'' group members carrying mercury resistance genes tend to be clustered with Tn''502'' and Tn''512'' although several are intermingled with Tn''402'' IntI1 carrying transposons. This is perhaps not surprising since many Tn''5053'' members were selected using a BLAST search. A similar argument can be proposed for the three Tn''Pfu1'' related transposons which carry an IntI3 gene.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>A phylogenetic tree of the concatenated TniA,B,Q and R proteins ([[:File:Fig-Tn402.8.png|Fig. Tn402.8]]) indicated that Tn''5053'' group members carrying mercury resistance genes tend to be clustered with Tn''502'' and Tn''512'' although several are intermingled with Tn''402'' IntI1 carrying transposons. This is perhaps not surprising since many Tn''5053'' members were selected using a BLAST search. A similar argument can be proposed for the three Tn''Pfu1'' related transposons which carry an IntI3 gene.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Fig-Tn402.8.png|center|thumb|800x800px|'''Fig. Tn402.8.''' '''Phylogeny of Tn''402'' Family Transposition Enzymes.''' TnpA protein sequences retrieved from the TnCentral curated data set were aligned with MAFFT 7.309, and their best-fit evolutionary models were predicted with ProTest 3.2.4. A maximum-likelihood tree was reconstructed with RaxML 8.2.9 using a bootstrap value of 1,000. The final tree was visualized in [http://tree.bio.ed.ac.uk/software/figtree FigTree 1.4.4] and edited with [http://www.inkscape.org Inkscape 0.92.4]. The colored circles indicate which group the transposons belong to. The insert gives the color code for members of family members with IntI1, InI3 and those carrying mercury resistance genes. Tn included together with the accession numbers from which they were drawn are: Tn''402''-[https://www.ncbi.nlm.nih.gov/nuccore/U67194.4 U67194.4;] Tn''402.1''-[https://www.ncbi.nlm.nih.gov/nuccore/GQ857074.1 GQ857074.1]; Tn''402.2''-[https://www.ncbi.nlm.nih.gov/nuccore/CP045551 CP045551]; Tn''402.3''-[https://www.ncbi.nlm.nih.gov/nuccore/CP063457 CP063457]; Tn''402.4''-[https://www.ncbi.nlm.nih.gov/nuccore/MW150990 MW150990]; Tn''402.5''-[https://www.ncbi.nlm.nih.gov/nuccore/MH392234 MH392234]; Tn''402.6''-[https://www.ncbi.nlm.nih.gov/nuccore/KT345947 KT345947]; Tn''402.8''-[https://www.ncbi.nlm.nih.gov/nuccore/MF490433 MF490433]; Tn''402.10''-[https://www.ncbi.nlm.nih.gov/nuccore/CP040126 CP040126]; Tn''502''-[https://www.ncbi.nlm.nih.gov/nuccore/EU306743 EU306743]; Tn''512''-[https://www.ncbi.nlm.nih.gov/nuccore/EU306744 EU306744]; In_TnAs3-[https://www.ncbi.nlm.nih.gov/nuccore/CP000645.1 CP000645.1]; In31-[https://www.ncbi.nlm.nih.gov/nuccore/AJ223604 AJ223604]; In32-[https://www.ncbi.nlm.nih.gov/nuccore/L36547 L36547]; In34-[https://www.ncbi.nlm.nih.gov/nuccore/AY123253 AY123253]; In73-[https://www.ncbi.nlm.nih.gov/nuccore/KY978631 KY978631]; In_Tn1721.1-[https://www.ncbi.nlm.nih.gov/nuccore/HQ730118.1 HQ730118.1]; In0-[https://www.ncbi.nlm.nih.gov/nuccore/U49101 U49101]; In2-[https://www.ncbi.nlm.nih.gov/nuccore/AF071413 AF071413]; Tn''6112''-[https://www.ncbi.nlm.nih.gov/nuccore/HQ423158.1 HQ423158.1]; TnpRSB105-[https://www.ncbi.nlm.nih.gov/nuccore/DQ839391.1 DQ839391.1]; Tn''5053''-[https://www.ncbi.nlm.nih.gov/nuccore/L40585.1 L40585.1]; Tn''5053.1''-[https://www.ncbi.nlm.nih.gov/nuccore/CP002451 CP002451]; Tn''5053.2''-[https://www.ncbi.nlm.nih.gov/nuccore/CP024682 CP024682]; Tn''5053.3''-[https://www.ncbi.nlm.nih.gov/nuccore/GQ983559 GQ983559]; Tn''5053.4''-[https://www.ncbi.nlm.nih.gov/nuccore/CP001919 CP001919]; Tn''5053.6''-[https://www.ncbi.nlm.nih.gov/nuccore/JX469833 JX469833]; Tn''5053.7''-[https://www.ncbi.nlm.nih.gov/nuccore/AM261760 AM261760]; Tn''5053.8''-[https://www.ncbi.nlm.nih.gov/nuccore/CP017991 CP017991]; Tn''5053.9''-[https://www.ncbi.nlm.nih.gov/nuccore/CP048650 CP048650]; Tn''6008''-[https://www.ncbi.nlm.nih.gov/nuccore/EU591509.1 EU591509.1]; In_TnCfrpOZ172-[https://www.ncbi.nlm.nih.gov/nuccore/CP016763.1 CP016763.1]; Tn''6007''-[https://www.ncbi.nlm.nih.gov/nuccore/EU591509.1 EU591509.1]; Tn''5718''-[https://www.ncbi.nlm.nih.gov/nuccore/AJ304453 AJ304453]; Tn''5058''-[https://www.ncbi.nlm.nih.gov/nuccore/Y17897 Y17897]; Tn''Pfu1''-[https://www.ncbi.nlm.nih.gov/nuccore/LC331665.1 LC331665.1]; Tn''Pfu1.1''-[https://www.ncbi.nlm.nih.gov/nuccore/LC589064 LC589064]; Tn''Pfu1.2''-[https://www.ncbi.nlm.nih.gov/nuccore/LC589062 LC589062]; Tn''7017''-[https://www.ncbi.nlm.nih.gov/nuccore/NC_022242.1 NC_022242.1]; TnpHS87a-[https://www.ncbi.nlm.nih.gov/nuccore/KR106190.1 KR106190.1]; TnXorYNA12-[https://www.ncbi.nlm.nih.gov/nuccore/HQ662557.1 HQ662557.1]; TnpTB11-[https://www.ncbi.nlm.nih.gov/nuccore/AJ744860 AJ744860].]]</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[[File:Fig-Tn402.8.png|center|thumb|800x800px|'''Fig. Tn402.8.''' '''Phylogeny of Tn''402'' Family Transposition Enzymes.''' TnpA protein sequences retrieved from the TnCentral curated data set were aligned with MAFFT 7.309, and their best-fit evolutionary models were predicted with ProTest 3.2.4. A maximum-likelihood tree was reconstructed with RaxML 8.2.9 using a bootstrap value of 1,000. The final tree was visualized in [http://tree.bio.ed.ac.uk/software/figtree FigTree 1.4.4] and edited with [http://www.inkscape.org Inkscape 0.92.4]. The colored circles indicate which group the transposons belong to. The insert gives the color code for members of family members with IntI1, InI3 and those carrying mercury resistance genes. Tn included together with the accession numbers from which they were drawn are: Tn''402''-[https://www.ncbi.nlm.nih.gov/nuccore/U67194.4 U67194.4;] Tn''402.1''-[https://www.ncbi.nlm.nih.gov/nuccore/GQ857074.1 GQ857074.1]; Tn''402.2''-[https://www.ncbi.nlm.nih.gov/nuccore/CP045551 CP045551]; Tn''402.3''-[https://www.ncbi.nlm.nih.gov/nuccore/CP063457 CP063457]; Tn''402.4''-[https://www.ncbi.nlm.nih.gov/nuccore/MW150990 MW150990]; Tn''402.5''-[https://www.ncbi.nlm.nih.gov/nuccore/MH392234 MH392234]; Tn''402.6''-[https://www.ncbi.nlm.nih.gov/nuccore/KT345947 KT345947]; Tn''402.8''-[https://www.ncbi.nlm.nih.gov/nuccore/MF490433 MF490433]; Tn''402.10''-[https://www.ncbi.nlm.nih.gov/nuccore/CP040126 CP040126]; Tn''502''-[https://www.ncbi.nlm.nih.gov/nuccore/EU306743 EU306743]; Tn''512''-[https://www.ncbi.nlm.nih.gov/nuccore/EU306744 EU306744]; In_TnAs3-[https://www.ncbi.nlm.nih.gov/nuccore/CP000645.1 CP000645.1]; In31-[https://www.ncbi.nlm.nih.gov/nuccore/AJ223604 AJ223604]; In32-[https://www.ncbi.nlm.nih.gov/nuccore/L36547 L36547]; In34-[https://www.ncbi.nlm.nih.gov/nuccore/AY123253 AY123253]; In73-[https://www.ncbi.nlm.nih.gov/nuccore/KY978631 KY978631]; In_Tn1721.1-[https://www.ncbi.nlm.nih.gov/nuccore/HQ730118.1 HQ730118.1]; In0-[https://www.ncbi.nlm.nih.gov/nuccore/U49101 U49101]; In2-[https://www.ncbi.nlm.nih.gov/nuccore/AF071413 AF071413]; Tn''6112''-[https://www.ncbi.nlm.nih.gov/nuccore/HQ423158.1 HQ423158.1]; TnpRSB105-[https://www.ncbi.nlm.nih.gov/nuccore/DQ839391.1 DQ839391.1]; Tn''5053''-[https://www.ncbi.nlm.nih.gov/nuccore/L40585.1 L40585.1]; Tn''5053.1''-[https://www.ncbi.nlm.nih.gov/nuccore/CP002451 CP002451]; Tn''5053.2''-[https://www.ncbi.nlm.nih.gov/nuccore/CP024682 CP024682]; Tn''5053.3''-[https://www.ncbi.nlm.nih.gov/nuccore/GQ983559 GQ983559]; Tn''5053.4''-[https://www.ncbi.nlm.nih.gov/nuccore/CP001919 CP001919]; Tn''5053.6''-[https://www.ncbi.nlm.nih.gov/nuccore/JX469833 JX469833]; Tn''5053.7''-[https://www.ncbi.nlm.nih.gov/nuccore/AM261760 AM261760]; Tn''5053.8''-[https://www.ncbi.nlm.nih.gov/nuccore/CP017991 CP017991]; Tn''5053.9''-[https://www.ncbi.nlm.nih.gov/nuccore/CP048650 CP048650]; Tn''6008''-[https://www.ncbi.nlm.nih.gov/nuccore/EU591509.1 EU591509.1]; In_TnCfrpOZ172-[https://www.ncbi.nlm.nih.gov/nuccore/CP016763.1 CP016763.1]; Tn''6007''-[https://www.ncbi.nlm.nih.gov/nuccore/EU591509.1 EU591509.1]; Tn''5718''-[https://www.ncbi.nlm.nih.gov/nuccore/AJ304453 AJ304453]; Tn''5058''-[https://www.ncbi.nlm.nih.gov/nuccore/Y17897 Y17897]; Tn''Pfu1''-[https://www.ncbi.nlm.nih.gov/nuccore/LC331665.1 LC331665.1]; Tn''Pfu1.1''-[https://www.ncbi.nlm.nih.gov/nuccore/LC589064 LC589064]; Tn''Pfu1.2''-[https://www.ncbi.nlm.nih.gov/nuccore/LC589062 LC589062]; Tn''7017''-[https://www.ncbi.nlm.nih.gov/nuccore/NC_022242.1 NC_022242.1]; TnpHS87a-[https://www.ncbi.nlm.nih.gov/nuccore/KR106190.1 KR106190.1]; TnXorYNA12-[https://www.ncbi.nlm.nih.gov/nuccore/HQ662557.1 HQ662557.1]; TnpTB11-[https://www.ncbi.nlm.nih.gov/nuccore/AJ744860 AJ744860].]]</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>When considered individually, the different gene products are very closely related: TniA (Fig. Tn402.<del class="diffchange diffchange-inline">8A; '''XXX-YYY''' identity, '''ZZZ-AAA''' similar</del>); TniB (Fig. Tn402.<del class="diffchange diffchange-inline">8B; '''XXX-YYY''' identity, '''ZZZ-AAA''' similar</del>); TniQ (<del class="diffchange diffchange-inline">(</del>Fig. Tn402.<del class="diffchange diffchange-inline">8C; '''XXX-YYY''' identity, '''ZZZ-AAA''' similar</del>) and TniR (Fig. Tn402.<del class="diffchange diffchange-inline">8D; '''XXX-YYY''' identity, '''ZZZ-AAA''' similar</del>).</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>When considered individually, the different gene products are very closely related: TniA (<ins class="diffchange diffchange-inline">[[:File:Fig-Tn402.9a.png|</ins>Fig. Tn402.<ins class="diffchange diffchange-inline">9]] see the slide show below</ins>); TniB (<ins class="diffchange diffchange-inline">[[:File:Fig-Tn402.9a.png|</ins>Fig. Tn402.<ins class="diffchange diffchange-inline">9]] see the slide show below</ins>); TniQ (<ins class="diffchange diffchange-inline">[[:File:Fig-Tn402.9a.png|</ins>Fig. Tn402.<ins class="diffchange diffchange-inline">9]] see the slide show below</ins>) and TniR (<ins class="diffchange diffchange-inline">[[:File:Fig-Tn402.9a.png|</ins>Fig. Tn402.<ins class="diffchange diffchange-inline">9]] see the slide show below</ins>).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The fact that the TniA,B,Q and R proteins are highly conserved ([[:File:Fig-Tn402.9a.png|Fig. Tn402.9]]) whereas the DNA sequences are more variable (see [[:File:Fig-Tn402.11.png|Fig. Tn402.11]] and [[:File:Fig-Tn402.13.png|Fig. Tn402.13]]) (and therefore the DNA variability mainly introduces synonymous mutations together with some conservative amino acid changes; [[:File:Fig-Tn402.9a.png|Fig. Tn402.9]]) suggests either that the phylogenetic branches are quite recent or that there is significant selective pressure on the protein ensemble.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The fact that the TniA,B,Q and R proteins are highly conserved ([[:File:Fig-Tn402.9a.png|Fig. Tn402.9]]) whereas the DNA sequences are more variable (see [[:File:Fig-Tn402.11.png|Fig. Tn402.11]] and [[:File:Fig-Tn402.13.png|Fig. Tn402.13]]) (and therefore the DNA variability mainly introduces synonymous mutations together with some conservative amino acid changes; [[:File:Fig-Tn402.9a.png|Fig. Tn402.9]]) suggests either that the phylogenetic branches are quite recent or that there is significant selective pressure on the protein ensemble.</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l189" >Line 189:</td>
<td colspan="2" class="diff-lineno">Line 189:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:Fig-Tn402.17a.png|center|thumb|800x800px|'''Fig. Tn402.17.''' '''Insertion sequence specificity. Res subsequences are shown in green boxes.''' Vertical arrowheads show individual insertions. These are colored according to the different inserted TE. '''A)''' insertions of <del class="diffchange diffchange-inline">Tn402 </del>into and near <del class="diffchange diffchange-inline">to </del>the par site of plasmid RP1 which functions in assuring separation of plasmid dimers and their correct segregation. <del class="diffchange diffchange-inline">(Kamali-Moghaddam and Sundström 2000) </del>'''B)''' Insertions of different family members into the par site of RP1 (BN000925). Tn''502''; Tn''502''DtniQ; Tn''512''; In0; In2 <ref name=":34" />.]]</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:Fig-Tn402.17a.png|center|thumb|800x800px|'''Fig. Tn402.17.''' '''Insertion sequence specificity. Res subsequences are shown in green boxes.''' Vertical arrowheads show individual insertions. These are colored according to the different inserted TE. '''A)''' insertions of <ins class="diffchange diffchange-inline">Tn''402'' </ins>into and near the <ins class="diffchange diffchange-inline">''</ins>par<ins class="diffchange diffchange-inline">'' </ins>site of plasmid RP1 which functions in assuring separation of plasmid dimers and their correct segregation <ins class="diffchange diffchange-inline"><ref name=":9" /></ins>. <ins class="diffchange diffchange-inline"> </ins>'''B)''' Insertions of different family members into the par site of RP1 (<ins class="diffchange diffchange-inline">[https://www.ncbi.nlm.nih.gov/nuccore/BN000925 </ins>BN000925<ins class="diffchange diffchange-inline">]</ins>). Tn''502''; Tn''502''DtniQ; Tn''512''; In0; In2 <ref name=":34" />.]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Tn''2521'', a multi-resistant transposon identified in the chromosome of ''[[wikipedia:Pseudomonas_aeruginosa|Pseudomonas aeruginosa]]'' clinical isolates in 1982 <ref name=":31"><nowiki><pubmed>PMC220297</pubmed></nowiki></ref> was also observed to have marked integration site-specificity in transposition into IncP plasmids (in this case R18-1, identical to R68). Tn''2521'' was later shown to be an integron (In33) without ''tni'' genes and its insertion point in the R18-18::Tn''2521'' example examined was shown to have inserted at the R18-18 equivalent to RP1/RP4 site ''resII'' <ref name=":25" />. Moreover, additional analysis of the flanking sequences of other integron structures indicated that insertion occurred in the ''res'' sites of several [[Transposons families/Tn3 family|Tn''3'' family transposon]] <ref><nowiki><pubmed>PMC90776</pubmed></nowiki></ref>: In28 in the ''resI'' region of Tn''1403''; In4 abutting ''resI'' in Tn''1696''; In1 upstream of ''resP'' in R46 ( <ref><nowiki><pubmed>6264522</pubmed></nowiki></ref>, also called TP120 <ref><nowiki><pubmed>325391</pubmed></nowiki></ref>), the region expected to include the plasmid ''res'' site which has an adjacent functional resolvase-like gene, ''uvp1'' <ref><nowiki><pubmed>9648746</pubmed></nowiki></ref>, and is similar to and can undergo recombination with that of Tn''1'' <ref><nowiki><pubmed>6306704</pubmed></nowiki></ref><ref name=":35"><nowiki><pubmed>3020154</pubmed></nowiki></ref><ref>Dodd HM, Bennett PM. The R46 Site-specific Recombination System is a Homoiogue of the Tn3 and gamma delta (Tn1000) Cointegrate Resolution System.</ref>. In these examples, the inserted integron platform does not contain the set of Tn''402'' transposition enzymes. This implies either that transposition preceded decay of the transposition genes or that their products were provided ''in trans'' from another transposon in the cell as is suggested by transposition of In33 (Tn''2521''). A further example, however, is the insertion into Tn''1721'' of an integron with a full complement of Tn''402'' transposition enzymes ([https://www.ncbi.nlm.nih.gov/nuccore/HQ730118 HQ730118]) <ref name=":27" />.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Tn''2521'', a multi-resistant transposon identified in the chromosome of ''[[wikipedia:Pseudomonas_aeruginosa|Pseudomonas aeruginosa]]'' clinical isolates in 1982 <ref name=":31"><nowiki><pubmed>PMC220297</pubmed></nowiki></ref> was also observed to have marked integration site-specificity in transposition into IncP plasmids (in this case R18-1, identical to R68). Tn''2521'' was later shown to be an integron (In33) without ''tni'' genes and its insertion point in the R18-18::Tn''2521'' example examined was shown to have inserted at the R18-18 equivalent to RP1/RP4 site ''resII'' <ref name=":25" />. Moreover, additional analysis of the flanking sequences of other integron structures indicated that insertion occurred in the ''res'' sites of several [[Transposons families/Tn3 family|Tn''3'' family transposon]] <ref><nowiki><pubmed>PMC90776</pubmed></nowiki></ref>: In28 in the ''resI'' region of Tn''1403''; In4 abutting ''resI'' in Tn''1696''; In1 upstream of ''resP'' in R46 ( <ref><nowiki><pubmed>6264522</pubmed></nowiki></ref>, also called TP120 <ref><nowiki><pubmed>325391</pubmed></nowiki></ref>), the region expected to include the plasmid ''res'' site which has an adjacent functional resolvase-like gene, ''uvp1'' <ref><nowiki><pubmed>9648746</pubmed></nowiki></ref>, and is similar to and can undergo recombination with that of Tn''1'' <ref><nowiki><pubmed>6306704</pubmed></nowiki></ref><ref name=":35"><nowiki><pubmed>3020154</pubmed></nowiki></ref><ref>Dodd HM, Bennett PM. The R46 Site-specific Recombination System is a Homoiogue of the Tn3 and gamma delta (Tn1000) Cointegrate Resolution System.</ref>. In these examples, the inserted integron platform does not contain the set of Tn''402'' transposition enzymes. This implies either that transposition preceded decay of the transposition genes or that their products were provided ''in trans'' from another transposon in the cell as is suggested by transposition of In33 (Tn''2521''). A further example, however, is the insertion into Tn''1721'' of an integron with a full complement of Tn''402'' transposition enzymes ([https://www.ncbi.nlm.nih.gov/nuccore/HQ730118 HQ730118]) <ref name=":27" />.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
</table>TnCentralhttps://tncentral.ncc.unesp.br/TnPedia/index.php?title=Transposons_families/Tn402_family&diff=1992&oldid=prevTnCentral: /* Insertion Sites: res Hunters */2021-03-16T21:14:28Z<p><span dir="auto"><span class="autocomment">Insertion Sites: res Hunters</span></span></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr class="diff-title" lang="en">
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 21:14, 16 March 2021</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l81" >Line 81:</td>
<td colspan="2" class="diff-lineno">Line 81:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>A similar ambiguity arises with TniB. A DNA alignment ([[:File:Fig-Tn402.7.png|Fig. Tn402.7]] '''A'''), however, not only shows a high degree of identity but suggests that there may be some form of translational coupling in the expression of TniA and TniB. There are potential TniB GTG start codons 1bp upstream and 2bp downstream of the predicted TniA termination codon (TAG) <ref name=":11"><nowiki><pubmed>PMC6728339</pubmed></nowiki></ref>. </div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>A similar ambiguity arises with TniB. A DNA alignment ([[:File:Fig-Tn402.7.png|Fig. Tn402.7]] '''A'''), however, not only shows a high degree of identity but suggests that there may be some form of translational coupling in the expression of TniA and TniB. There are potential TniB GTG start codons 1bp upstream and 2bp downstream of the predicted TniA termination codon (TAG) <ref name=":11"><nowiki><pubmed>PMC6728339</pubmed></nowiki></ref>. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Although we have chosen to use the downstream GTG (Fig. Tn402.9 TniB), it should be remembered that if the upstream GTG is used, TniB would include an N-terminal extension of three amino acids, MVA. Note that the 2 mutations shown in the figure are synonymous (ATT/ATC) and conservative (GAC/GAA) respectively.</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Although we have chosen to use the downstream GTG (<ins class="diffchange diffchange-inline">[[:File:Fig-Tn402.9a.png|</ins>Fig. Tn402.9<ins class="diffchange diffchange-inline">]] </ins>TniB), it should be remembered that if the upstream GTG is used, TniB would include an N-terminal extension of three amino acids, MVA. Note that the 2 mutations shown in the figure are synonymous (ATT/ATC) and conservative (GAC/GAA) respectively.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=====TniQ Expression, Translation Initiation and Translational Coupling with TniB=====</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>=====TniQ Expression, Translation Initiation and Translational Coupling with TniB=====</div></td></tr>
<tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l194" >Line 194:</td>
<td colspan="2" class="diff-lineno">Line 194:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>This type of insertion site-specificity was also observed for Tn''5053'' <ref name=":0" />, again using RP1 and an RP4 derivative plasmid target. Sequence analysis showed that the insertions were flanked by a 5 bp direct target repeat as expected <ref name=":0" /><ref name=":22" />. A survey of conjugative plasmids (cited in <ref name=":32"><nowiki><pubmed>10476039</pubmed></nowiki></ref>) identified only a single example which acted as an efficient Tn''5053'' target. This plasmid carried Tn''701'', a close relative of Tn''1721'' and The Tn''5053'' insertion were reported to have occurred in the ''res'' site of this transposon. Introduction of Tn''701'' or Tn''1721'' into plasmids not generally active targets such as R387, pOXGen or pBR322, these became efficient Tn''5053'' targets <ref name=":32" />. In addition, a database search using the first 50 bp of the ends of Tn''5053'' and Tn''402'' as the query sequences revealed a number of Tn junctions in which the IRi ends were located upstream of resolvase-like genes <ref name=":32" />.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>This type of insertion site-specificity was also observed for Tn''5053'' <ref name=":0" />, again using RP1 and an RP4 derivative plasmid target. Sequence analysis showed that the insertions were flanked by a 5 bp direct target repeat as expected <ref name=":0" /><ref name=":22" />. A survey of conjugative plasmids (cited in <ref name=":32"><nowiki><pubmed>10476039</pubmed></nowiki></ref>) identified only a single example which acted as an efficient Tn''5053'' target. This plasmid carried Tn''701'', a close relative of Tn''1721'' and The Tn''5053'' insertion were reported to have occurred in the ''res'' site of this transposon. Introduction of Tn''701'' or Tn''1721'' into plasmids not generally active targets such as R387, pOXGen or pBR322, these became efficient Tn''5053'' targets <ref name=":32" />. In addition, a database search using the first 50 bp of the ends of Tn''5053'' and Tn''402'' as the query sequences revealed a number of Tn junctions in which the IRi ends were located upstream of resolvase-like genes <ref name=":32" />.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>In these studies <ref name=":32" />, Insertion of Tn''5035'' was observed experimentally using as target the cloned insertion-free ''res'' site of Tn''5044'' <ref><nowiki><pubmed>10875286</pubmed></nowiki></ref> together with the native TnpR gene. Very strong clustering was observed between resI and resII and all but one insertion occurred in the same orientation (Fig. Tn402.<del class="diffchange diffchange-inline">17C</del>). Moreover, when cloned and in the absence of TnpR, the ''res'' sites of Tn''1721'' <ref><nowiki><pubmed>1312499</pubmed></nowiki></ref>, Tn''5036'' <ref name=":21" />, Tn''5051'' (no sequence available) <ref name=":21" /><ref name=":32" /> and Tn''5059'' ([https://www.ncbi.nlm.nih.gov/nuccore/Y10102 Y10102, partial sequence]) were not attractive to Tn''5035'' insertion, however, when TnpR from Tn''1721'' was supplied in trans, efficient targeted insertion occurred and sequence analysis showed strong clustering (Fig. Tn402.<del class="diffchange diffchange-inline">17D</del>). This behavior rules out the possibility that expression (transcription or translation) of TnpR in its natural proximity to res provides a suitable integration substrate. A similar requirement for the ParA resolvase was reported for insertion into the RP1 res. Surprisingly target attractiveness appeared to be limited to the Tn''21'' branch of the [[Transposons families/Tn3 family|Tn''3'' family]] <ref name=":20" /><ref><nowiki><pubmed>6312271</pubmed></nowiki></ref> neither Tn''1'' nor Tn''1000'' were found to be efficient targets for Tn''5053'' insertion.</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>In these studies <ref name=":32" />, Insertion of Tn''5035'' was observed experimentally using as target the cloned insertion-free ''res'' site of Tn''5044'' <ref><nowiki><pubmed>10875286</pubmed></nowiki></ref> together with the native TnpR gene. Very strong clustering was observed between resI and resII and all but one insertion occurred in the same orientation (<ins class="diffchange diffchange-inline">[[:File:Fig-Tn402.17b.png|</ins>Fig. Tn402.<ins class="diffchange diffchange-inline">17]] '''C'''</ins>). Moreover, when cloned and in the absence of TnpR, the ''res'' sites of Tn''1721'' <ref><nowiki><pubmed>1312499</pubmed></nowiki></ref>, Tn''5036'' <ref name=":21" />, Tn''5051'' (no sequence available) <ref name=":21" /><ref name=":32" /> and Tn''5059'' ([https://www.ncbi.nlm.nih.gov/nuccore/Y10102 Y10102, partial sequence]) were not attractive to Tn''5035'' insertion, however, when TnpR from Tn''1721'' was supplied in trans, efficient targeted insertion occurred and sequence analysis showed strong clustering (<ins class="diffchange diffchange-inline">[[:File:Fig-Tn402.17a.png|</ins>Fig. Tn402.<ins class="diffchange diffchange-inline">17]] '''D'''</ins>). This behavior rules out the possibility that expression (transcription or translation) of TnpR in its natural proximity to res provides a suitable integration substrate. A similar requirement for the ParA resolvase was reported for insertion into the RP1 res. Surprisingly target attractiveness appeared to be limited to the Tn''21'' branch of the [[Transposons families/Tn3 family|Tn''3'' family]] <ref name=":20" /><ref><nowiki><pubmed>6312271</pubmed></nowiki></ref> neither Tn''1'' nor Tn''1000'' were found to be efficient targets for Tn''5053'' insertion.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Tn''402'' family members with mercury resistance genes, Tn''502'' and Tn''512'', have also been found to show quasi-identical insertion specificity in an RP1 res target <ref name=":24" /> as Tn''402'' itself (Fig. Tn402.<del class="diffchange diffchange-inline">17B</del>). Remarkably, the insertion site observed in resII was identical to that observed for Tn''402''/Tn''5090'' (cluster 1 in [[:File:Fig-Tn402.17a.png|Fig. Tn402.17]] '''A''')<ref name=":30" />. The authors also address the potential mobility of integron platforms, such as In0 ([https://www.ncbi.nlm.nih.gov/nuccore/U49101 U49101]) and In2 ([https://www.ncbi.nlm.nih.gov/nuccore/AF071413 AF071413]) which do not contain a full set of Tn''402'' ''tni'' genes but carry both IRi and IRt ends and are found with flanking 5 bp direct target repeats. Transposition of In2 from its location in Tn''21'' and of In0 from its location in plasmid pVS1 was tested by supplying Tn''502'' ''tni'' genes ''in trans''. Transposition occurred at significant frequencies if both ''tni'' and the RP1 ''par'' site were present. Moreover, the vast majority of insertions occurred in the expected orientation at the cluster 1 site (Fig. Tn402.<del class="diffchange diffchange-inline">17B</del>). One puzzling observation was that although neither In0 nor In2 include a Tn402 res site never-the-less, the transposition products had undergone resolution.</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Tn''402'' family members with mercury resistance genes, Tn''502'' and Tn''512'', have also been found to show quasi-identical insertion specificity in an RP1 res target <ref name=":24" /> as Tn''402'' itself (<ins class="diffchange diffchange-inline">[[:File:Fig-Tn402.17a.png|</ins>Fig. Tn402.<ins class="diffchange diffchange-inline">17]] '''B'''</ins>). Remarkably, the insertion site observed in resII was identical to that observed for Tn''402''/Tn''5090'' (cluster 1 in [[:File:Fig-Tn402.17a.png|Fig. Tn402.17]] '''A''')<ref name=":30" />. The authors also address the potential mobility of integron platforms, such as In0 ([https://www.ncbi.nlm.nih.gov/nuccore/U49101 U49101]) and In2 ([https://www.ncbi.nlm.nih.gov/nuccore/AF071413 AF071413]) which do not contain a full set of Tn''402'' ''tni'' genes but carry both IRi and IRt ends and are found with flanking 5 bp direct target repeats. Transposition of In2 from its location in Tn''21'' and of In0 from its location in plasmid pVS1 was tested by supplying Tn''502'' ''tni'' genes ''in trans''. Transposition occurred at significant frequencies if both ''tni'' and the RP1 ''par'' site were present. Moreover, the vast majority of insertions occurred in the expected orientation at the cluster 1 site (<ins class="diffchange diffchange-inline">[[:File:Fig-Tn402.17a.png|</ins>Fig. Tn402.<ins class="diffchange diffchange-inline">17]] '''B'''</ins>). One puzzling observation was that although neither In0 nor In2 include a Tn402 res site never-the-less, the transposition products had undergone resolution.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Complementation by a resident Tn''402'' family transposon would provide an explanation for transposition of Tn''2521'' (In33) into the R18-1 <ref name=":31" /> ''res'' site even though it does not contain a single Tn''402'' transposition gene <ref name=":25" />.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Complementation by a resident Tn''402'' family transposon would provide an explanation for transposition of Tn''2521'' (In33) into the R18-1 <ref name=":31" /> ''res'' site even though it does not contain a single Tn''402'' transposition gene <ref name=":25" />.</div></td></tr>
</table>TnCentral