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IS Families/IS982 family

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General

The IS982 family has over 70 entries in ISfinder from over 40 bacterial and archaeal species. In the case of ISLpl4 from Lactobacillus plantarum identical copies have been detected in Leuconostoc mesenteroides, Oenococcus oeni, and Lactobacillus sakei indicating horizontal gene transfer. Historically, a single IS982 family member, ISPfu3, was first identified in the archaea from the archaea Pyrococcus furiosus and this was later joined by ISTli1 from Thermococcus litoralis (both mrmbero of the order Thermococcales).

A phylogenetic tree of the transposase of family members (Fig. IS982.1) shows three deeply branching clades (a, b and c) which broadly correspond to those identified by Fayad et al. [1]. They can be further subdivided into several subclades (ai; aii and aii; bi, which includes streptococci and lactococci; and bii which contain bacilli and lactobacilli)

Fig. IS982.1. A dendrogram of 63 IS982 family members. The figure shows three major clades. The surrounding colored circles and the insert indicate the clades identified by Fayad et al. [1]


A recent extensive BLAST search in the non-redundant public databases using transposase examples from each of the major clades and a threshold of greater than 30% identity [1] indicated that members of this family are widely distributed over bacterial and archaeal genera and species. This includes the Euryarchaeota Methanotorris formicicus and Methanocaldococcus bathoardescencs and alpha-, beta-, gama- and epsilon-protobacteria, firmicutes, deinococci, cyanobacteria and actinobacteria. In total 265 new genera containing IS982-related elements were ideidentified,though the IS copies from this analysis were not further characterized [1].

Like many IS, members of this family are able to increase gene expression presumably by supplying a promoter or by formation of a hybrid promoter on insertion upstream of a resident gene. At least two members, IS982B [2] and IS1187 [see [3][4][5]] can provide a -35 hexamer in their right IR capable of forming a hybrid promoter with a resident -10 .

A recent review of this family [1] lists a number of additional elements including ISAba4, ISAba9, ISAba47 and ISAba825 whose insertion can lead to increased carbapenem resistance in Acinetobacter baumannii [6][7][8][9][10][11][12]. In addition, as also pointed out by Fayad et al. [1], insertion of members of the family which lead to gene inactivation has been observed to result in: increased antibiotic resistance (vancomycin and teicoplanin by IS19 [13] and ISEfm1 [14] in Enterococcus faecium; carbapenem by ISAba825 disruption of the CarO membrane protein [15] in A. baumannii); reduced antibiotic resistance (daptomycin in Enterococcus faecium; [16]); or in decreased virulence (ISSa4 in S. agalactiae [17]; ISScr1 in Streptococcus criceti [18]; IS195 in Porphyromonas gingivalis [19]).

Organization

IS982 family members are between 962 and 1155 bp long (Fig.IS982.2), carry similar terminal IRs of between 18 and 35 bp with conserved ends: 5’-ACCC-3’ and generate DR of 6 to 8 bp e.g. ISSa4 from Streptococcus agalactiae generates 7 bp (or perhaps 9 bp) flanking DRs [17] and ISCce1 from Clostridium cellulovorans is flanked by 7 bp repeats [20]. There is some evidence that the IR consensus varies slightly according to each IS subgroup [1] (Fig.IS982.2a)

Fig. IS982. Top: Distribution of IS length (base pairs) of IS982 family members. The number of examples used in the sample is shown above each column. Bottom: Left (IRL) and right IRR inverted terminal repeats are shown in WebLogo format.
Fig. IS982.2.a. Left (IRL) inverted terminal repeats are shown in WebLogo format.

Family members encode a single orf of between of 271 and 313 amino acids with a possible DDE motif but without a convincing conserved downstream K/R residue. They exhibit between 33% and 44% amino acid identity. The identities are shown in the alignment included in (Fig.IS982.3 a and b) together with the DDE motif suggested by Fayad et al. [1]. The N-terminal domain, which includes a potential HTH DNA binding motif [1] is not shown.

Fig. IS982.3.a. Alignment of the proposed IS982 family transposase catalytic domain. The conserved DDE triad identified by Fayad et al [1] is indicated by vertical red arrows. The N-terminal HTH domain is not shown.


Although the Tpase of a majority of members occupies a single reading phase, there are several examples in which the gene is distributed over two phases. It has been reported that a +1 nucleotide insertion in the Tpase orf of an ISLpl4 from Oenococcus oeni may undergo programmed translational frameshifting at a low rate [21]. Although this must be confirmed, it would represent the first functional case of +1 frameshifting in IS. The Tpase of archaeal element ISPfu3 is also distributed over two phases. ISPfu3 carries a potential transcriptional frameshift signal A7 (PTR) present in all five copies, suggesting that ISPfu3 is active.


Fig. IS982.3.b. Alignment of the proposed IS982 family transposase catalytic domain. The conserved DDE triad identified by Fayad et al [1] is indicated by vertical red arrows. The N-terminal HTH domain is not shown.

Mechanism

At present, there is no information concerning the transposition mechanism of this family.

Bibliography

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Fayad et al.. IS982 and kin: new insights into an old IS family. Mobile DNA. 2020. 11. pp. 24. doi: 10.1186/s13100-020-00221-z. PMID: 32641975.
  2. ↑ López de Felipe et al.. Transcriptional activation of the citrate permease P gene of Lactococcus lactis biovar diacetylactis by an insertion sequence-like element present in plasmid pCIT264. Molecular & general genetics : MGG. 1996. 250. pp. 428-36. doi: 10.1007/BF02174031. PMID: 8602160.
  3. ↑ Kato et al.. New insertion sequence elements in the upstream region of cfiA in imipenem-resistant Bacteroides fragilis strains. Antimicrobial agents and chemotherapy. 2003. 47. pp. 979-85. doi: 10.1128/AAC.47.3.979-985.2003. PMID: 12604530.
  4. ↑ Sóki et al.. Molecular analysis of the carbapenem and metronidazole resistance mechanisms of Bacteroides strains reported in a Europe-wide antibiotic resistance survey. International journal of antimicrobial agents. 2013. 41. pp. 122-5. doi: 10.1016/j.ijantimicag.2012.10.001. PMID: 23158541.
  5. ↑ Podglajen et al.. Multiple mobile promoter regions for the rare carbapenem resistance gene of Bacteroides fragilis. Journal of bacteriology. 2001. 183. pp. 3531-5. doi: 10.1128/JB.183.11.3531-3535.2001. PMID: 11344163.
  6. ↑ Lopes et al.. ISAba825 controls the expression of the chromosomal bla(OXA-51-like) and the plasmid borne bla(OXA-58) gene in clinical isolates of Acinetobacter baumannii isolated from the USA. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 2012. 18. pp. E446-51. doi: 10.1111/j.1469-0691.2012.03979.x. PMID: 22862829.
  7. ↑ Ravasi et al.. ISAba825, a functional insertion sequence modulating genomic plasticity and bla(OXA-58) expression in Acinetobacter baumannii. Antimicrobial agents and chemotherapy. 2011. 55. pp. 917-20. doi: 10.1128/AAC.00491-10. PMID: 21098239.
  8. ↑ Khorsi et al.. High prevalence of multidrug-resistance in Acinetobacter baumannii and dissemination of carbapenemase-encoding genes blaOXA-23-like, blaOXA-24-like and blaNDM-1 in Algiers hospitals. Asian Pacific journal of tropical medicine. 2015. 8. pp. 438-46. doi: 10.1016/j.apjtm.2015.05.011. PMID: 26194827.
  9. ↑ Villalón et al.. Epidemiology of the Acinetobacter-derived cephalosporinase, carbapenem-hydrolysing oxacillinase and metallo-β-lactamase genes, and of common insertion sequences, in epidemic clones of Acinetobacter baumannii from Spain. The Journal of antimicrobial chemotherapy. 2013. 68. pp. 550-3. doi: 10.1093/jac/dks448. PMID: 23143900.
  10. ↑ Levy-Blitchtein et al.. Emergence and spread of carbapenem-resistant Acinetobacter baumannii international clones II and III in Lima, Peru. Emerging microbes & infections. 2018. 7. pp. 119. doi: 10.1038/s41426-018-0127-9. PMID: 29970918.
  11. ↑ Potron et al.. Genetic and biochemical characterization of the first extended-spectrum CARB-type beta-lactamase, RTG-4, from Acinetobacter baumannii. Antimicrobial agents and chemotherapy. 2009. 53. pp. 3010-6. doi: 10.1128/AAC.01164-08. PMID: 19380596.
  12. ↑ Figueiredo et al.. Overexpression of the naturally occurring blaOXA-51 gene in Acinetobacter baumannii mediated by novel insertion sequence ISAba9. Antimicrobial agents and chemotherapy. 2009. 53. pp. 4045-7. doi: 10.1128/AAC.00292-09. PMID: 19564367.
  13. ↑ Perichon et al.. Glycopeptide-resistant Enterococcus faecium BM4416 is a VanD-type strain with an impaired D-Alanine:D-Alanine ligase. Antimicrobial agents and chemotherapy. 2000. 44. pp. 1346-8. doi: 10.1128/AAC.44.5.1346-1348.2000. PMID: 10770774.
  14. ↑ Boyd et al.. Molecular characterization of the vanD gene cluster and a novel insertion element in a vancomycin-resistant enterococcus isolated in Canada. Journal of clinical microbiology. 2000. 38. pp. 2392-4. doi: 10.1128/JCM.38.6.2392-2394.2000. PMID: 10835012.
  15. ↑ Mussi et al.. Acquisition of resistance to carbapenems in multidrug-resistant clinical strains of Acinetobacter baumannii: natural insertional inactivation of a gene encoding a member of a novel family of beta-barrel outer membrane proteins. Antimicrobial agents and chemotherapy. 2005. 49. pp. 1432-40. doi: 10.1128/AAC.49.4.1432-1440.2005. PMID: 15793123.
  16. ↑ Sinel et al.. Sequential steps of daptomycin resistance in Enterococcus faecium and reversion to hypersusceptibility through IS-mediated inactivation of the liaFSR operon. The Journal of antimicrobial chemotherapy. 2016. 71. pp. 2793-7. doi: 10.1093/jac/dkw229. PMID: 27353469.
  17. ↑ 17.0 17.1 Spellerberg et al.. Identification of a novel insertion sequence element in Streptococcus agalactiae. bspeller@imib.rwth-aachen.de. Gene. 2000. 241. pp. 51-6. doi: 10.1016/s0378-1119(99)00469-2. PMID: 10607898.
  18. ↑ Tamura et al.. Characterization of Streptococcus criceti insertion sequence ISScr1. Genes & genetic systems. 2012. 87. pp. 153-60. doi: 10.1266/ggs.87.153. PMID: 22976390.
  19. ↑ Lewis & Macrina. IS195, an insertion sequence-like element associated with protease genes in Porphyromonas gingivalis. Infection and immunity. 1998. 66. pp. 3035-42. doi: 10.1128/IAI.66.7.3035-3042.1998. PMID: 9632563.
  20. ↑ Tamaru & Doi. The engL gene cluster of Clostridium cellulovorans contains a gene for cellulosomal manA. Journal of bacteriology. 2000. 182. pp. 244-7. doi: 10.1128/JB.182.1.244-247.2000. PMID: 10613891.
  21. ↑ de Las Rivas et al.. Characterization of ISLpl4, a functional insertion sequence in Lactobacillus plantarum. Gene. 2005. 363. pp. 202-10. doi: 10.1016/j.gene.2005.09.005. PMID: 16278055.


How to Cite?

TnPedia Team. (2025). TnPedia: IS982 Family of Prokaryotic Insertion Sequences. Zenodo. https://doi.org/10.5281/zenodo.15636291

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