Difference between revisions of "IS Families/IS630 family"

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====Original Identification====
 
====Original Identification====
IS630 was first identified in the Shigella sonnei genome<ref><nowiki><pubmed>2824781</pubmed></nowiki></ref>. Another transpositionally active IS630 family member which showed similarity to the Tc1 eukaryotic transposon family<ref><nowiki><pubmed>9305771</pubmed></nowiki></ref> has received most attention was subsequently identified in the cyanobacterium Synechocystis PCC6803 and called ISS1987<ref><nowiki><pubmed>9305771</pubmed></nowiki></ref> or ISTcSa and subsequently renamed ISY100<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref>.
+
IS''630'' was first identified in the ''[[wikipedia:Shigella_sonnei|Shigella sonnei]]'' genome<ref><nowiki><pubmed>2824781</pubmed></nowiki></ref>. Another transpositionally active IS''630'' family member which showed similarity to the Tc1 eukaryotic transposon family<ref><nowiki><pubmed>9305771</pubmed></nowiki></ref> has received most attention was subsequently identified in the [[wikipedia:Cyanobacteria|cyanobacterium]] ''[[wikipedia:Synechocystis|Synechocystis]]'' PCC6803 and called IS''S1987''<ref><nowiki><pubmed>9305771</pubmed></nowiki></ref> or IS''TcSa'' and subsequently renamed IS''Y100''<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref>.
  
 
====Distribution====
 
====Distribution====
There are over 200 members from over 80 bacterial and archaeal genomes. IS630 itself has been used to cluster subspecies of Aeromonas salmonicida by high copy number IS630 restriction fragment length polymorphism (HCN-IS630-RFLP)<ref><nowiki><pubmed>23406017</pubmed></nowiki></ref>.
+
There are over 200 members from over 80 bacterial and archaeal genomes. IS''630'' itself has been used to cluster subspecies of ''[[wikipedia:Aeromonas_salmonicida|Aeromonas salmonicida]]'' by high copy number IS''630'' restriction fragment length polymorphism (HCN-IS630-RFLP)<ref><nowiki><pubmed>23406017</pubmed></nowiki></ref>.
  
 
====Organization====
 
====Organization====
Members are between 950 and 1250 bp in length with an average of 1100 bp (Fig. IS630.2). The have short terminal IRs (Fig. IS630.1) and generally include a single orf.  However, in about nearly half of the members, the Tpase orf is distributed over two reading frames suggesting that it may be produced as a fusion protein by frameshifting. These include IS895 and ISRm2011-2, identified several decades ago, which appear to contain two consecutive open reading frames<ref><nowiki><pubmed>1653219</pubmed></nowiki></ref>.
+
Members are between 950 and 1250 bp in length with an average of 1100 bp ([[:File:Fig. IS630.2.png|Fig. IS630.2]]). The have short terminal IRs ([[:File:Fig. IS630.1.png|Fig. IS630.1]]) and generally include a single orf.  However, in about nearly half of the members, the Tpase orf is distributed over two reading frames suggesting that it may be produced as a fusion protein by frameshifting. These include IS''895'' and IS''Rm2011-2'', identified several decades ago, which appear to contain two consecutive open reading frames<ref><nowiki><pubmed>1653219</pubmed></nowiki></ref>.
[[Image:Fig. IS630.1.png|thumb|center|850px|Fig. IS630.1]]
+
[[Image:Fig. IS630.1.png|thumb|center|600x600px|'''Fig. IS630.1.''' IS''630'' DDE domains and IRs.|alt=]]
[[Image:Fig. IS630.2.png|thumb|center|850px|Fig. IS630.2]]
+
[[Image:Fig. IS630.2.png|thumb|center|600x600px|'''Fig. IS630.2.''' IS630 length distribution.|alt=]]
  
Other members family carry a single long reading frame. Three elements (IS870, ISAr1, and ISRf1) show more than 70% identity, two (IS1066 and ISRj1) show about 50% identity with each other and 40% with the other members, while two show less than 20% identity between themselves and with the other members. This is reflected in the relatively low similarity of their IRs (Fig. IS630.1).  
+
Other members family carry a single long reading frame. Three elements (IS''870'', IS''Ar1'', and IS''Rf1'') show more than 70% identity, two (IS''1066'' and IS''Rj1'') show about 50% identity with each other and 40% with the other members, while two show less than 20% identity between themselves and with the other members. This is reflected in the relatively low similarity of their IRs ([[:File:Fig. IS630.1.png|Fig. IS630.1]]).  
It is interesting to note that the three most closely related on the basis of Tpase similarities (IS870, ISRf1, and ISAr1) appear not to carry translation termination codons for the Tpase gene. However, insertion into the specific target site, CTAG, with concomitant duplication (of either 2 or 4 base pairs) generates a TAG termination codon in phase with the Tpase gene. The influence of this arrangement on the transposition of the IS elements has yet to be determined.
+
It is interesting to note that the three most closely related on the basis of Tpase similarities (IS''870'', IS''Rf1'', and IS''Ar1'') appear not to carry translation termination codons for the Tpase gene. However, insertion into the specific target site, CTAG, with concomitant duplication (of either 2 or 4 base pairs) generates a TAG termination codon in phase with the Tpase gene. The influence of this arrangement on the transposition of the IS elements has yet to be determined.
  
Of the ISY100 copies in the Synechocystis PCC6803 genome, around 50%  were found to include a single orf while the others included 2 smaller orfs which had been split by the presence of an additional A nucleotide in a stretch of 9 As<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref>. While it is possible that these IS copies are inactive, it is also possible that a full length transposase is expressed by frameshifting. Although the absence of the characteristic secondary structure signals associated with -1 programmed translational frameshifting suggest that this is unlikely, it remains possible that a transcriptional mechanism is operating. One member, ISRm2011-2 from Rhizobium meliloti, also carries a group II intron which appears to be active in vivo<ref><nowiki><pubmed>9680217</pubmed></nowiki></ref>.  
+
Of the IS''Y100'' copies in the ''[[wikipedia:Synechocystis|Synechocystis]]'' PCC6803 genome, around 50%  were found to include a single orf while the others included 2 smaller orfs which had been split by the presence of an additional A nucleotide in a stretch of 9 As<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref>. While it is possible that these IS copies are inactive, it is also possible that a full length transposase is expressed by frameshifting. Although the absence of the characteristic secondary structure signals associated with -1 programmed translational frameshifting suggest that this is unlikely, it remains possible that a transcriptional mechanism is operating. One member, IS''Rm2011-2'' from ''[[wikipedia:Sinorhizobium_meliloti|Rhizobium meliloti]]'', also carries a [[wikipedia:Group_II_intron|group II intron]] which appears to be active ''in vivo''<ref><nowiki><pubmed>9680217</pubmed></nowiki></ref>.  
  
The IS630 family is related to the Tc1/mariner family of eukaryotic TE particularly at the level of the DDE signature<ref><nowiki><pubmed>9305771</pubmed></nowiki></ref> (Fig. IS630.1). There is also an N-terminal HTH motif<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref> (Fig. IS630.3) whose function in binding ISY100 ends has been verified in vitro<ref><nowiki><pubmed>17680987</pubmed></nowiki></ref>. Moreover, IS630 and the Tc1/mariner families target similar sequences, have similar DR and transposition of both involves cleavage two nucleotides inside the 5’ ends<ref><nowiki><pubmed>17680987</pubmed></nowiki></ref><ref><nowiki><pubmed>8556864</pubmed></nowiki></ref>.
+
The IS''630'' family is related to the Tc1/mariner family of eukaryotic TE particularly at the level of the DDE signature<ref><nowiki><pubmed>9305771</pubmed></nowiki></ref> ([[:File:Fig. IS630.1.png|Fig. IS630.1]]). There is also an N-terminal [[wikipedia:Helix-turn-helix|HTH motif]]<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref> ([[:File:Fig. IS630.3.png|Fig. IS630.3]]) whose function in binding IS''Y100'' ends has been verified ''in vitro''<ref><nowiki><pubmed>17680987</pubmed></nowiki></ref>. Moreover, IS''630'' and the Tc1/mariner families target similar sequences, have similar DR and transposition of both involves cleavage two nucleotides inside the 5’ ends<ref><nowiki><pubmed>17680987</pubmed></nowiki></ref><ref><nowiki><pubmed>8556864</pubmed></nowiki></ref>.
[[Image:Fig. IS630.3.png|thumb|center|850px|Fig. IS630.3 The amino acid sequence of transposase encoded by ISY100 is shown below the nucleotide sequence. The TA sequences adjacent to both ends of the ISY100 sequence indicate the target site sequence duplicated on the transposition of ISY100. The arrows show the terminal IRs, IRL and IRR. The underlined amino acid sequence is the possible DNA-binding region with a helix-turn-helix motif. Amino acid residues of the DDE motif are circled.]]
+
[[Image:Fig. IS630.3.png|thumb|center|600x600px|'''Fig. IS630.3.''' The amino acid sequence of transposase encoded by IS''Y100'' is shown below the nucleotide sequence. The TA sequences adjacent to both ends of the IS''Y100'' sequence indicate the target site sequence duplicated on the transposition of IS''Y100''. The arrows show the terminal IRs, IRL and IRR. The underlined amino acid sequence is the possible DNA-binding region with a helix-turn-helix motif. Amino acid residues of the DDE motif are circled.|alt=]]
  
Analysis of the Streptococcus pneumoniae genome revealed several hundred copies of short imperfect palindromic sequences (RUPs) whose ends show strong homology to those of a full length putative IS630-related element also present in the genome<ref><nowiki><pubmed>10537186</pubmed></nowiki></ref>. Comparison of empty and full sites from different S. pneumoniae strains indicated that, like most full-length members of the family, the RUPs are flanked by a TA dinucleotide target repeat. Although transposition of RUP sequences has yet to be demonstrated, they are structurally similar to the IS231-related MIC231 elements in B. thuringiensis (IS4 family) and to several eukaryote systems where many truncated copies of an element may exist together with a full length functional copy that is capable of complementing the non-autonomous copies to drive their transposition. Other IS630-based MITES have been identified in  Escherichia coli, Photorhabdus luminescens<ref><nowiki><pubmed>14528314</pubmed></nowiki></ref> and Yersinia pestis<ref><nowiki><pubmed>16963573</pubmed></nowiki></ref>.
+
Analysis of the ''[[wikipedia:Streptococcus_pneumoniae|Streptococcus pneumoniae]]'' genome revealed several hundred copies of short imperfect palindromic sequences (RUPs) whose ends show strong homology to those of a full length putative IS''630''-related element also present in the genome<ref><nowiki><pubmed>10537186</pubmed></nowiki></ref>. Comparison of empty and full sites from different ''[[wikipedia:Streptococcus_pneumoniae|S. pneumoniae]]'' strains indicated that, like most full-length members of the family, the RUPs are flanked by a TA dinucleotide target repeat. Although transposition of RUP sequences has yet to be demonstrated, they are structurally similar to the IS''231''-related MIC231 elements in ''[[wikipedia:Bacillus_thuringiensis|B. thuringiensis]]'' (see "[[IS Families/IS4 and related families|IS''4'' family]]") and to several eukaryote systems where many truncated copies of an element may exist together with a full length functional copy that is capable of complementing the non-autonomous copies to drive their transposition. Other IS''630''-based MITES have been identified in  ''[[wikipedia:Escherichia_coli|Escherichia coli]]'', ''[[wikipedia:Photorhabdus_luminescens|Photorhabdus luminescens]]''<ref><nowiki><pubmed>14528314</pubmed></nowiki></ref> and ''[[wikipedia:Yersinia_pestis|Yersinia pestis]]''<ref><nowiki><pubmed>16963573</pubmed></nowiki></ref>.
  
 
====Insertion Specificity====
 
====Insertion Specificity====
 
Family members show high target specificity inserting into and duplicating a TA dinucleotide with a preference for the sequence 5’-NTAN-3’<ref><nowiki><pubmed>1655702</pubmed></nowiki></ref>. Since the cleavages of the non-transferred strand occur 2 nts within the 5’ end of the IS, repair of the donor molecule after excision of the IS can result in a 2 bp scar at the excision site.  
 
Family members show high target specificity inserting into and duplicating a TA dinucleotide with a preference for the sequence 5’-NTAN-3’<ref><nowiki><pubmed>1655702</pubmed></nowiki></ref>. Since the cleavages of the non-transferred strand occur 2 nts within the 5’ end of the IS, repair of the donor molecule after excision of the IS can result in a 2 bp scar at the excision site.  
  
Detailed studies concerning the target DNA sequence have been carried out in the case of IS630<ref><nowiki><pubmed>2163390</pubmed></nowiki></ref>. The target sequence was determined before and after insertion and the results suggested that insertion generated a duplication of an invariant target TA dinucleotide. That this dinucleotide does not form an integral part of the IS was investigated by site-specific mutagenesis of the transposon donor to eliminate the terminal TA. Transposition of the IS from the mutated donor molecule resulted in insertions which all exhibited a flanking TA direct repeat. This clearly demonstrates that insertion results in the duplication of the central TA dinucleotide<ref><nowiki><pubmed>2163390</pubmed></nowiki></ref>. Further analysis demonstrated that IS630 exhibits a strong preference for a 5'-CTAG-3' target sequence<ref><nowiki><pubmed>1655702</pubmed></nowiki></ref>. Point mutation of the CTAG target sites reduced or eliminated their attractiveness as insertion hotspots. The two preferred insertion sites were identified in plasmid ColE1 corresponded to TA sequences in the inverted repeats of a 13-base-pair stem region of the [rho]-dependent transcription terminator. IS630 is flanked by TA, and in vitro mutagenesis of the flanking TA did not affect further transposition activity, the ability to insert preferentially into the TA within the 13-base-pair inverted repeat or to duplicate its target sequence<ref><nowiki><pubmed>1655702</pubmed></nowiki></ref><ref><nowiki><pubmed>2163390</pubmed></nowiki></ref>.
+
Detailed studies concerning the target DNA sequence have been carried out in the case of IS''630''<ref><nowiki><pubmed>2163390</pubmed></nowiki></ref>. The target sequence was determined before and after insertion and the results suggested that insertion generated a duplication of an invariant target TA dinucleotide. That this dinucleotide does not form an integral part of the IS was investigated by site-specific mutagenesis of the transposon donor to eliminate the terminal TA. Transposition of the IS from the mutated donor molecule resulted in insertions which all exhibited a flanking TA direct repeat. This clearly demonstrates that insertion results in the duplication of the central TA dinucleotide<ref><nowiki><pubmed>2163390</pubmed></nowiki></ref>. Further analysis demonstrated that IS''630'' exhibits a strong preference for a 5'-CTAG-3' target sequence<ref><nowiki><pubmed>1655702</pubmed></nowiki></ref>. Point mutation of the CTAG target sites reduced or eliminated their attractiveness as insertion hotspots. The two preferred insertion sites were identified in plasmid ColE1 corresponded to TA sequences in the inverted repeats of a 13-base-pair stem region of the [[wikipedia:Rho_factor|[rho]-dependent transcription terminator]]. IS''630'' is flanked by TA, and ''in vitro'' mutagenesis of the flanking TA did not affect further transposition activity, the ability to insert preferentially into the TA within the 13-base-pair inverted repeat or to duplicate its target sequence<ref><nowiki><pubmed>1655702</pubmed></nowiki></ref><ref><nowiki><pubmed>2163390</pubmed></nowiki></ref>.
  
All known insertions of these elements are consistent with a TA duplication. In two insertions of the Agrobacterium vitis element IS870 it was possible to determine the target sequence prior to insertion. This was found to carry a single CTAG copy [14], an observation which remains consistent with a simple TA dinucleotide target duplication. We assume that all members of this family generate an identical (TA) target duplication and the tips of the elements have therefore been defined accordingly in ISfinder.  
+
All known insertions of these elements are consistent with a TA duplication. In two insertions of the ''[[wikipedia:Allorhizobium_vitis|Agrobacterium vitis]]'' element IS''870'' it was possible to determine the target sequence prior to insertion. This was found to carry a single CTAG copy, an observation which remains consistent with a simple TA dinucleotide target duplication. We assume that all members of this family generate an identical (TA) target duplication and the tips of the elements have therefore been defined accordingly in [https://isfinder.biotoul.fr/ ISfinder].  
  
 
====Mechanism====
 
====Mechanism====
In vivo studies<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref> demonstrated that, when supplied with transposase, the IS630 family member, ISY100 (ISTcSa) first identified in Synechocystis sp. PCC6803<ref><nowiki><pubmed>9305771</pubmed></nowiki></ref> generates linear forms which terminate exactly at  the 3’ ends but which lack 2 nucleotides at the 5’ ends resulting in a 2 base overhang. Both in vivo<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref> and in vitro using circular plasmid DNA<ref><nowiki><pubmed>17680987</pubmed></nowiki></ref>. This is also a characteristic of transposition of the eukaryote Tc1 transposon<ref><nowiki><pubmed>10431195</pubmed></nowiki></ref>. Although IS circles had been observed, as for Tc1  these are thought to be dead-end products and not transposition intermediates<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref>.
+
''In vivo'' studies<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref> demonstrated that, when supplied with transposase, the IS''630'' family member, IS''Y100'' (IS''TcSa'') first identified in ''[[wikipedia:Synechocystis|Synechocystis]]'' sp. PCC6803<ref><nowiki><pubmed>9305771</pubmed></nowiki></ref> generates linear forms which terminate exactly at  the 3’ ends but which lack 2 nucleotides at the 5’ ends resulting in a 2 base overhang. Both ''in vivo''<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref> and ''in vitro'' using circular plasmid DNA<ref><nowiki><pubmed>17680987</pubmed></nowiki></ref>. This is also a characteristic of transposition of the eukaryote Tc1 transposon<ref><nowiki><pubmed>10431195</pubmed></nowiki></ref>. Although IS circles had been observed, as for Tc1  these are thought to be dead-end products and not transposition intermediates<ref><nowiki><pubmed>12193627</pubmed></nowiki></ref>.
 
   
 
   
In the case of IS630 itself, while direct insertions can be observed at reasonable frequencies, no cointegrates could be detected<ref><nowiki><pubmed>2163390</pubmed></nowiki></ref>.
+
In the case of IS''630'' itself, while direct insertions can be observed at reasonable frequencies, no cointegrates could be detected<ref><nowiki><pubmed>2163390</pubmed></nowiki></ref>.
  
IS630 transposition has been addressed in vitro using ISY100<ref><nowiki><pubmed>17680987</pubmed></nowiki></ref>. The Tpase was shown to specifically bind ISY100 IR using an N-terminal domain containing two potential HTH motifs. It is the only protein required for ISY100 excision and integration and introduces double-strand breaks on mini-ISY100 on a supercoiled DNA substrate. Tc1/mariner element transposition has also been extensively studied in vitro<ref><nowiki><pubmed>8556864</pubmed></nowiki></ref> and a Tpase structural model is available [e.g. <ref><nowiki><pubmed>12535535</pubmed></nowiki></ref><ref><nowiki><pubmed>16511570</pubmed></nowiki></ref>]. IS630 Tpase cleaves exactly at the 3’ (transferred strand) IS ends and two nucleotides inside the 5’ (non-transferred strand) ends. Cleavage is less precise on linear substrates. Both single-end and, less frequently, double-end insertion occur in vitro in a TA-target-specific manner<ref><nowiki><pubmed>19965773</pubmed></nowiki></ref>. Transposition does not involve a hairpin intermediate.  
+
IS''630'' transposition has been addressed ''in vitro'' using IS''Y100''<ref><nowiki><pubmed>17680987</pubmed></nowiki></ref>. The Tpase was shown to specifically bind IS''Y100'' IR using an N-terminal domain containing two potential [[wikipedia:Helix-turn-helix|HTH motifs]]. It is the only protein required for IS''Y100'' excision and integration and introduces double-strand breaks on mini-IS''Y100'' on a supercoiled DNA substrate. Tc1/mariner element transposition has also been extensively studied ''in vitro''<ref><nowiki><pubmed>8556864</pubmed></nowiki></ref> and a Tpase structural model is available [e.g. <ref><nowiki><pubmed>12535535</pubmed></nowiki></ref><ref><nowiki><pubmed>16511570</pubmed></nowiki></ref>]. IS''630'' Tpase cleaves exactly at the 3’ (transferred strand) IS ends and two nucleotides inside the 5’ (non-transferred strand) ends. Cleavage is less precise on linear substrates. Both single-end and, less frequently, double-end insertion occur ''in vitro'' in a TA-target-specific manner<ref><nowiki><pubmed>19965773</pubmed></nowiki></ref>. Transposition does not involve a hairpin intermediate.  
 
 
The related eukaryote Tc1 element is known to transpose by a cut and paste mechanism and leaves a “footprint” (additional bases in the original donor site) on excision (Fig. IS630.4). When purified vector backbone produced by transposase mediated cleavage of ISY100 in vitro was circularised In vitro, gel- produced by transposase-mediated cleavage of pISY100 -kan was efficiently circularized with T4 DNA ligase the predicted TATATA (i.e. a footprint) junction sequence was recovered after transformation into E. coli.
 
[[Image:Fig. IS630.4.png|thumb|center|850px|Fig. IS630.4]]
 
 
 
It has also been demonstrated that fusion of a zinc-finger DNA-binding domain of Zif268 to the ISY100 transposase C-terminus targets integration into TA dinucleotides positioned 6-17 bp to one side of a Zif268 binding site. The targeting specificity can be changed with Zif268 variants which recognize other sequences<ref><nowiki><pubmed>19965773</pubmed></nowiki></ref>.
 
<br/>
 
  
 +
The related eukaryote Tc1 element is known to transpose by a cut and paste mechanism and leaves a “footprint” (additional bases in the original donor site) on excision ([[:File:Fig. IS630.4.png|Fig. IS630.4]]). When purified vector backbone produced by transposase mediated cleavage of IS''Y100'' ''in vitro'' was circularised ''In vitro'', gel- produced by transposase-mediated cleavage of pISY100 -kan was efficiently circularized with T4 DNA ligase the predicted TATATA (i.e. a footprint) junction sequence was recovered after transformation into ''E. coli''.
 +
[[Image:Fig. IS630.4.png|thumb|center|600x600px|'''Fig. IS630.4.''' Proposed IS''Y100'' transposition mechanism.|alt=]]
  
 +
It has also been demonstrated that fusion of a zinc-finger DNA-binding domain of Zif268 to the IS''Y100'' transposase C-terminus targets integration into TA dinucleotides positioned 6-17 bp to one side of a Zif268 binding site. The targeting specificity can be changed with Zif268 variants which recognize other sequences<ref><nowiki><pubmed>19965773</pubmed></nowiki></ref>.
 +
<br />
 
==Bibliography==
 
==Bibliography==
<references/>
+
<references />

Revision as of 15:12, 1 June 2020

Original Identification

IS630 was first identified in the Shigella sonnei genome[1]. Another transpositionally active IS630 family member which showed similarity to the Tc1 eukaryotic transposon family[2] has received most attention was subsequently identified in the cyanobacterium Synechocystis PCC6803 and called ISS1987[3] or ISTcSa and subsequently renamed ISY100[4].

Distribution

There are over 200 members from over 80 bacterial and archaeal genomes. IS630 itself has been used to cluster subspecies of Aeromonas salmonicida by high copy number IS630 restriction fragment length polymorphism (HCN-IS630-RFLP)[5].

Organization

Members are between 950 and 1250 bp in length with an average of 1100 bp (Fig. IS630.2). The have short terminal IRs (Fig. IS630.1) and generally include a single orf. However, in about nearly half of the members, the Tpase orf is distributed over two reading frames suggesting that it may be produced as a fusion protein by frameshifting. These include IS895 and ISRm2011-2, identified several decades ago, which appear to contain two consecutive open reading frames[6].

Fig. IS630.1. IS630 DDE domains and IRs.
Fig. IS630.2. IS630 length distribution.

Other members family carry a single long reading frame. Three elements (IS870, ISAr1, and ISRf1) show more than 70% identity, two (IS1066 and ISRj1) show about 50% identity with each other and 40% with the other members, while two show less than 20% identity between themselves and with the other members. This is reflected in the relatively low similarity of their IRs (Fig. IS630.1). It is interesting to note that the three most closely related on the basis of Tpase similarities (IS870, ISRf1, and ISAr1) appear not to carry translation termination codons for the Tpase gene. However, insertion into the specific target site, CTAG, with concomitant duplication (of either 2 or 4 base pairs) generates a TAG termination codon in phase with the Tpase gene. The influence of this arrangement on the transposition of the IS elements has yet to be determined.

Of the ISY100 copies in the Synechocystis PCC6803 genome, around 50% were found to include a single orf while the others included 2 smaller orfs which had been split by the presence of an additional A nucleotide in a stretch of 9 As[7]. While it is possible that these IS copies are inactive, it is also possible that a full length transposase is expressed by frameshifting. Although the absence of the characteristic secondary structure signals associated with -1 programmed translational frameshifting suggest that this is unlikely, it remains possible that a transcriptional mechanism is operating. One member, ISRm2011-2 from Rhizobium meliloti, also carries a group II intron which appears to be active in vivo[8].

The IS630 family is related to the Tc1/mariner family of eukaryotic TE particularly at the level of the DDE signature[9] (Fig. IS630.1). There is also an N-terminal HTH motif[10] (Fig. IS630.3) whose function in binding ISY100 ends has been verified in vitro[11]. Moreover, IS630 and the Tc1/mariner families target similar sequences, have similar DR and transposition of both involves cleavage two nucleotides inside the 5’ ends[12][13].

Fig. IS630.3. The amino acid sequence of transposase encoded by ISY100 is shown below the nucleotide sequence. The TA sequences adjacent to both ends of the ISY100 sequence indicate the target site sequence duplicated on the transposition of ISY100. The arrows show the terminal IRs, IRL and IRR. The underlined amino acid sequence is the possible DNA-binding region with a helix-turn-helix motif. Amino acid residues of the DDE motif are circled.

Analysis of the Streptococcus pneumoniae genome revealed several hundred copies of short imperfect palindromic sequences (RUPs) whose ends show strong homology to those of a full length putative IS630-related element also present in the genome[14]. Comparison of empty and full sites from different S. pneumoniae strains indicated that, like most full-length members of the family, the RUPs are flanked by a TA dinucleotide target repeat. Although transposition of RUP sequences has yet to be demonstrated, they are structurally similar to the IS231-related MIC231 elements in B. thuringiensis (see "IS4 family") and to several eukaryote systems where many truncated copies of an element may exist together with a full length functional copy that is capable of complementing the non-autonomous copies to drive their transposition. Other IS630-based MITES have been identified in Escherichia coli, Photorhabdus luminescens[15] and Yersinia pestis[16].

Insertion Specificity

Family members show high target specificity inserting into and duplicating a TA dinucleotide with a preference for the sequence 5’-NTAN-3’[17]. Since the cleavages of the non-transferred strand occur 2 nts within the 5’ end of the IS, repair of the donor molecule after excision of the IS can result in a 2 bp scar at the excision site.

Detailed studies concerning the target DNA sequence have been carried out in the case of IS630[18]. The target sequence was determined before and after insertion and the results suggested that insertion generated a duplication of an invariant target TA dinucleotide. That this dinucleotide does not form an integral part of the IS was investigated by site-specific mutagenesis of the transposon donor to eliminate the terminal TA. Transposition of the IS from the mutated donor molecule resulted in insertions which all exhibited a flanking TA direct repeat. This clearly demonstrates that insertion results in the duplication of the central TA dinucleotide[19]. Further analysis demonstrated that IS630 exhibits a strong preference for a 5'-CTAG-3' target sequence[20]. Point mutation of the CTAG target sites reduced or eliminated their attractiveness as insertion hotspots. The two preferred insertion sites were identified in plasmid ColE1 corresponded to TA sequences in the inverted repeats of a 13-base-pair stem region of the [rho]-dependent transcription terminator. IS630 is flanked by TA, and in vitro mutagenesis of the flanking TA did not affect further transposition activity, the ability to insert preferentially into the TA within the 13-base-pair inverted repeat or to duplicate its target sequence[21][22].

All known insertions of these elements are consistent with a TA duplication. In two insertions of the Agrobacterium vitis element IS870 it was possible to determine the target sequence prior to insertion. This was found to carry a single CTAG copy, an observation which remains consistent with a simple TA dinucleotide target duplication. We assume that all members of this family generate an identical (TA) target duplication and the tips of the elements have therefore been defined accordingly in ISfinder.

Mechanism

In vivo studies[23] demonstrated that, when supplied with transposase, the IS630 family member, ISY100 (ISTcSa) first identified in Synechocystis sp. PCC6803[24] generates linear forms which terminate exactly at the 3’ ends but which lack 2 nucleotides at the 5’ ends resulting in a 2 base overhang. Both in vivo[25] and in vitro using circular plasmid DNA[26]. This is also a characteristic of transposition of the eukaryote Tc1 transposon[27]. Although IS circles had been observed, as for Tc1 these are thought to be dead-end products and not transposition intermediates[28].

In the case of IS630 itself, while direct insertions can be observed at reasonable frequencies, no cointegrates could be detected[29].

IS630 transposition has been addressed in vitro using ISY100[30]. The Tpase was shown to specifically bind ISY100 IR using an N-terminal domain containing two potential HTH motifs. It is the only protein required for ISY100 excision and integration and introduces double-strand breaks on mini-ISY100 on a supercoiled DNA substrate. Tc1/mariner element transposition has also been extensively studied in vitro[31] and a Tpase structural model is available [e.g. [32][33]]. IS630 Tpase cleaves exactly at the 3’ (transferred strand) IS ends and two nucleotides inside the 5’ (non-transferred strand) ends. Cleavage is less precise on linear substrates. Both single-end and, less frequently, double-end insertion occur in vitro in a TA-target-specific manner[34]. Transposition does not involve a hairpin intermediate.

The related eukaryote Tc1 element is known to transpose by a cut and paste mechanism and leaves a “footprint” (additional bases in the original donor site) on excision (Fig. IS630.4). When purified vector backbone produced by transposase mediated cleavage of ISY100 in vitro was circularised In vitro, gel- produced by transposase-mediated cleavage of pISY100 -kan was efficiently circularized with T4 DNA ligase the predicted TATATA (i.e. a footprint) junction sequence was recovered after transformation into E. coli.

Fig. IS630.4. Proposed ISY100 transposition mechanism.

It has also been demonstrated that fusion of a zinc-finger DNA-binding domain of Zif268 to the ISY100 transposase C-terminus targets integration into TA dinucleotides positioned 6-17 bp to one side of a Zif268 binding site. The targeting specificity can be changed with Zif268 variants which recognize other sequences[35].

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