IS Families

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Prokaryotic Insertion Sequences (IS)
1. IS1 family
2. IS1595 family
3. IS3 family [updated on 04/10/22]
4. IS481 family
5. IS4 and related families
5a. IS701 family
5b. ISH3 family
5c. IS1634 family
6. IS5 and related IS1182 families
7. IS6 family [updated on 05/21/22]
8. IS21 family
9. IS30 family [updated on 05/21/22]
10. IS66 family
11. IS110 and IS1111 families
12. IS256 family
13. IS630 family
14. IS982 family
15. IS1202 family [New Family - under construction]
16. IS1380 family
17. ISAs1 family
18. ISL3 family
19. ISAzo13 family
20. IS607 family
21. IS91 and related ISCR families
22. IS200/IS605 family [updated on 02/21/22]


Quick Access Table: Main Characteristics and Summary of Each IS family and Sub-Groups

Characteristics of insertion sequence families. Abbreviations: DR, duplication repeat; IS, insertion sequence; ORF, open reading frame.
Families Sub-Groups Typical size-range (bp) DR (bp) Ends IRs No ORFs Frameshift Catalytic residues Mechanism
IS1 740–1180 8–9 GGnnnTG Y 2 ORFAB DDE copy-and-paste and cointegrate
single ORF 800–1200 0–9 N 1
ISMhu11 900–4600 0–10 Y 2 ORFAB
IS1595 ISPna2 1000–1150 8 GGCnnTG Y 1 DDNK copy-and-paste (?)
ISPna2+pass 1500–2600 8 1+pass
ISH4 1000 8 CGCTCTT 1 DDNK
IS1016 700–745 7–9 GGGgctg DDEK
IS1595 900–1100 8 CcTGATT DDNK+ER4R7
ISSod11 1000–1100 8 nnnGcnTATC DDHK+ER4R7
ISNwi1 1080–1200 8 ggnnatTAT DDEK+ER4
ISNwi1+pass 1750–4750 8 1+pass
ISNha5 3450–7900 8 CGGnnTT 1 DDER/K
IS3 IS150 1200–1600 3–4 TG Y 2 ORFAB DDE copy-and-paste
IS407 1100–1400 4 TG
IS51 1000–1400 3–4 TG
IS3 1150–1750 3–4 TGa/g
IS2 1300–1400 5 TG
IS481 950–1300 4–15 TGT Y 1 DDE copy-and-paste (?)
IS4 IS10 1200–1350 9 CT Y 1 DDE hairpin intermediate cut-and-paste
IS50 1350–1550 8–9 C hairpin intermediate
ISPepr1 1500–1600 7–8 -T-AA ?
IS4 1400–1600 10–13 -AAT ?
IS4Sa 1150–1750 8–10 CA ?
ISH8 1400–1800 10 ?
IS231 1450–5400 10–12 CAT 1 or + * *passenger genes
IS701 1400–1550 4 Y 1 DDE
ISAba11
ISH3 1225–1500 4–5 C-GT Y 1 DDE
IS1634 1500–2000 5–6 C Y 1 DDE
IS5 IS903 950–1150 9 GG Y 1 DDE
ISL2 850–1200 2–3
ISH1 900–1150 8 -GC
IS5 1000–1500 4 Ga/g
IS1031 850–1050 3 GAa/g
IS427 800–1000 2–4 Ga/g 2 ORFAB
IS1182 1330–1950 0–60 Y 1 DDE
IS6 700–900 8 GG Y 1 DDE co-integrate
IS21 1750–2600 4–8 TG Y 2 * DDE
IS30 1000–1700 2–3 Y 1 DDE copy-and-paste
IS66 2000–3000 8–9 GTAA Y 3* DDE*
ISBst12 1350–1900 1 DDE
IS256 1200–1500 8–9 Ga/g Y 1 DDE copy-and-paste
IS1249 1300 0–10 GG
ISC1250 1250 0–9 GG
ISH6 1450 8 GGT Y 1 DDE
ISLre2 1500–2000 9 Y 1 DDE
ISKra4 ISAzba1 1400–2900 0 Y 1 or + * DDE
ISMich2 1250–1400 8 GGG 1 or 2 ORFAB
ISKra4 1400–3700 9 GGG 1 or + *
IS630 1000–1400 2* Y 1 or 2 ORFAB DDE cut-and-paste
IS982 1000 3–9 AC Y 1 DDE
IS1380 1550–2000 4–5 CC Y 1 DDE
ISAs1 1200–1500 8–10 CAGGG Y 1
ISL3 1300–2300 8 GG Y 1
Tn3 >3000 0 GGGG Y >1 DDE co-integrate
ISAzo13 1250–2200 0–4 Ga/g Y 1
IS110 1200–1550 0 N 1 DEDD
IS1111 Y*
IS91 1500–2000 0 N 1 HUH/Y2 rolling-circle
IS200/IS605 IS200 600–750 0 0 1* HUH/Y1 peel-and-paste
IS605 1300–2000 2* HUH/Y1**
IS607 1700–2500 0 N 2* Serine**
IS1202 1400–1700 5 TGT Y 1 DDE
ISNCY * IS892 1600 0–8 CTAG Y 2 ORFAB
ISLbi1 1400–1500 5 Y 1
ISMae2 1400–2400 9 CAG Y 1
ISPlu15 800–1000 0 N 1
ISA1214 1000–1200 8–12 Y 2
ISC1217 1200 6–8 TAG Y 1
ISM1 1300–1600 8–9 Y 1
ISDol1 1600–1900 6–7 Y 1 DDE
* ISNCY = Insertion Sequence Not Classified Yet