Difference between revisions of "Glossary"

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==See also==
<br />
==='''The logic behind the TEs nomenclature and naming attribution'''===
<br />''<code>"Why should researchers contemplate naming a newly identified TE?"''<br />"Mainly to name it, so the discoverer and other researchers can specifically refer to it.<br />
<br/ >  
This is becoming increasingly important as our understanding about the influence of TEs upon their hosts becomes more apparent"<ref name=":6"><pubmed>31624505</pubmed></nowiki></ref></code>.
<htmlet nocache="yes" >foobar</htmlet>
<br /><br />
*'''Prokaryotic TE nomenclature and naming attribution'''
Transposable elements were discovered by [[wikipedia:Barbara_McClintock|Barbara McClintock]] during experiments conducted in 1944 on maize. However, her discovery was met with less than enthusiastic reception by the genetic community <ref name=":4"><pubmed>31278665</pubmed></nowiki></ref> (for a full detailed history, please see Evelyn Fox Keller's acclaimed biography: [https://www.amazon.com.br/Feeling-Organism-10th-Aniversary-McClintock/dp/0805074589 A Feeling for the Organism, 10th Aniversary Edition: The Life and Work of Barbara McClintock]).
Only some decades later her discovery was brought back to life after the Szybalski group which in the early 1970s discovered the bacterial insertion sequences <ref name=":4" /><ref><nowiki><pubmed>4567155</pubmed></nowiki></ref>. After that discovery, the picture of transposable elements started to change.
A committee assembled during the meeting on DNA Insertions at [https://www.cshl.edu/ Cold Spring Harbor] in 1976 proposed a set of rules to be used for the nomenclature of '''T'''ransposable '''E'''lements <ref name=":1"><pubmed>18778731</pubmed></nowiki></ref>. The first attempt to create an concise nomenclature system for '''T'''ransposable '''E'''lements started at [https://www.stanford.edu/ Stanford University] by Campbell and colleagues in 1979 <ref name=":0"><pubmed>467979</pubmed></nowiki></ref>. They classified the prokaryotes elements as simple IS elements to more complex Tn transposons, and self-replicating episomes. In addition, definitions and nomenclature rules for these three classes of prokaryotic TEs were specified <ref name=":0" /> ('''Table 1'''). ISs and TEs were named separately by having IS and Tn as a prefix, respectively, followed by a sequential number in italics such as IS''1'', IS''2'' and Tn''1'', Tn2, etc <ref name=":6" />. 
{| class="wikitable" style="width: 85%;"
|+'''Table 1.''' TE nomenclature rules defined by [https://pubmed.ncbi.nlm.nih.gov/467979/ Campbell et al., 1979]
!Naming rule
|'''IS elements'''
|IS elements contain no known genes unrelated to insertion functions and are shorter than 2kb.
|IS''1'', IS''2'', IS''3'', IS''4'', IS''5'', etc, with the numbers italicized
|'''Tn elements'''
|Tn are more complex, often containing two copies of an IS element. They are generally larger than 2kb, and contain additional genes unrelated to insertion functions.
|Tn''1'', Tn''2'', Tn''3'', etc, with the numbers italicized
|Episomes are complex, self-replicating elements, often containing IS and Tn elements. Examples include the phage λ and plasmid F of ''E. coli.''
| -
The allocation of numbers and database administration was carried out by the late [[wikipedia:Esther_Lederberg|Dr Esther Lederberg]] from Stanford University Medical School, CA, USA <ref name=":1" />. Lists for the registry of Tn number allocations were subsequently published <ref><nowiki><pubmed>3036649</pubmed></nowiki></ref>. The allocation of Tn numbers stopped with the retirement of Dr Lederberg and gradually a variety of rules were adopted for naming newly discovered transposons <ref name=":1" />. At the same time new types of transposable elements, such as the mobilizable and conjugative transposons, were being discovered <ref name=":1" />. Additionally, interactions between different elements including transposition and/or recombination events led to novel chimeric transposons. These exacerbated the nomenclature problem <ref name=":1" />. Subsequent nomenclature systems have become complicated, with different systems being adopted for related elements by different research groups <ref name=":1" />. Therefore, Robert and colleagues in 2008 proposed a new version of the early nomenclature system, but not including non-autonomous elements (such as integron cassettes and MITEs) <ref name=":1" /> ('''Table 2''').
{| class="wikitable" style="width: 85%;"
|+'''Table 2.''' Revised Nomenclature for Transposable Genetic Elements proposed by [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3836210/ Roberts et al., 2008].
!Type of transposable element <sup>a</sup>
|'''Composite transposons'''
|Flanked by IS elements. The transposase of the IS element is responsible for the catalysis of insertion and excision.
|'''Unit transposons'''
|Typical unit elements encode an enzyme involved in excision and integration (DD(35)E or tyrosine) often a site-specific recombinase or resolvase and one or several accessory (e.g. resistance) genes in one genetic unit.
|'''Conjugative transposons (CTns) / Integrative conjugative elements (ICEs)'''
|The conjugative transposons (CTns), also known as integrative conjugative elements (ICEs), carry genes for excision, conjugative transfer, and for integration within the new host genome. They carry a wide range of accessory genes, including antibiotic resistance
|'''Mobilisable transposons (MTns) / Integrative mobilisable elements (IMEs)'''
|The mobilizable transposons (MTns), also known as integrative mobilizable elements (IMEs), can be mobilized between bacterial cells by other “helper” elements that encode proteins involved in the formation of the conjugation pore or mating bridge. The MTns exploit these conjugation pores and generally provide their own DNA processing functions for intercellular transfer and subsequent transposition.
|'''Mobile genomic islands'''
|Some chromosomally integrated genomic islands encode tyrosine or serine site-specific recombinases that catalyze their own excision and integration but do not harbor genes involved in the transfer. They carry genes encoding for a range of phenotypes. The name of a genomic island reflects the phenotype it confers, e.g. pathogenicity islands encode virulence determinants (toxins, adhesins etc).
|'''Integrated or transposable prophage'''
|An integrated or transposable prophage is a phage genome inserted as part of the linear structure of the chromosome of a bacterium which is able to excise and insert from and into the genome.
|'''Integrated satellite prophage'''
|Bacteriophage genome inserted into that of the host which requires gene products from “helper” phages to complete its replication cycle
|'''Group I intron'''
|Small post-transcriptionally splicing (splicing occurs in the pre-mRNA), endonuclease encoding element. Will home to the allelic site
|'''Group II intron'''
|Small post-transcriptionally splicing (splicing occurs in the pre-mRNA), restriction endonuclease encoding element
|'''IStron intein'''
|Chimeric ribozyme consisting of a group I intron linked to an IS''605'' like transposase Small post-translational splicing (splicing occurs in the polypeptide), endonuclease encoding element. Will home to allelic site.
<sup>a</sup>; not all reported elements have been shown to be mobile
Following Roberts et al <ref name=":1" /> classification system, the [https://isfinder.biotoul.fr/ ISfinder] team ([https://scholar.google.com/citations?user=r8TYgVEAAAAJ&hl=pt-BR Mick Chandler] and [https://scholar.google.com/citations?user=WHAtfqcAAAAJ&hl=pt-BR Patricia Siguier]) and [https://scholar.google.com/citations?user=zb0GIgsAAAAJ&hl=pt-BR Jacques Mahillon] proposed a enhancement for this classification system, now including terms and definitions for MICs ('''M'''obile '''I'''nsertion '''C'''assette), composed of passenger genes but no transposase, and MITES ('''M'''iniature '''I'''nverted repeat '''T'''ransposable '''E'''lement), which are short IS-related elements with no internal open reading frame but which, like most ISs, transposons and MICs, include IS-like extremities. It is important to note that a single IS might in principle be represented in all four forms ('''Table 3''').
{| class="wikitable"
|+'''Table 3. IS classification system proposed by Siguier et al., 2008'''
!'''Passenger genes'''
|yes<br />
*'''Insertion Sequences nomenclature and naming attribution'''<br />Nowadays the IS nomenclature and naming attribution is controlled by [https://www-is.biotoul.fr/index.php ISfinder database] team <ref name=":5"><pubmed>16381877</pubmed></nowiki></ref>. They adopted a nomenclature similar to that of restriction enzymes <ref name=":5" />. Although this is not perfect, since some ISs are found in different species or even in different genera, the system is viable and has the advantage of indicating the host species rather than being confronted with a long series of numbers in the names as in the original nomenclature system <ref name=":0" /><ref name=":5" />. The original Campbell classification system assign blocks of IS numbers to individual scientists, groups or institutions<ref name=":0" />. The ISfinder database includes a listing of these original ISs numbers together with the last known address of the attribution <ref name=":5" /> (See [https://isfinder.biotoul.fr/reserved_blocks.php ISfinder page]). Moreover, ISfinder includes an online form for registering (requesting a name for) new ISs <ref name=":5" /> (See [https://isfinder.biotoul.fr/request_name_form.php ISfinder form]). Several journals now suggest that authors register their new IS elements with ISfinder before publication<ref name=":5" />. Since a unique name can be attributed, this avoids some of the confusion in the literature<ref name=":5" />.<br />
*'''Tn nomenclature and naming attribution'''<br />The Tn nomenclature and naming attribution is controlled by "[https://transposon.lstmed.ac.uk/ The Transposon Registry]" database <ref name=":6" />. The Transposon Registry is a nomenclature system for the assignment of Tn numbers for bacterial and archaeal autonomous TEs, including unit transposons, composite transposons, conjugative transposons (CTns)/Integrative Conjugative Elements (ICEs), Mobilisable transposons (MTns)/Integrative mobilizable elements (IMEs) and mobile genomic islands<ref name=":6" />. It excludes ISs, which are managed by ISfinder database and other TEs such as introns and inteins for which other databases already exist, and non-autonomous TEs such as integron cassettes and MITES <ref name=":6" />.<br />
*'''Bioinformatics approaches for TE identification and annotation in prokaryotes genomes'''
Most genome annotation pipelines developed to date are dedicated to the prediction and characterization of coding regions and their putative products, signal peptides, pseudogenes, and noncoding RNAs. Surprisingly, annotation of ubiquitous genomic features such as Mobile Genetic Elements (MGEs) is generally not fully addressed and/or is neglected in most of these pipelines, and thus MGEs are seldom well-characterized and annotated <ref name=":7"><pubmed>29277867</pubmed></nowiki></ref>.
To begin an analysis of the MGE content of a given genome, it is first necessary to identify and annotate ordinary genome features such as coding sequences (CDSs), rRNAs, and tRNAs <ref name=":7" /> . For MGE detection and annotation, it is essential to use a variety of approaches including additional specialized pipelines and software which are normally not implemented in a regular annotation pipeline <ref name=":7" />. Therefore, it is not a simple task and requires different approaches depending on the level of analysis. A recent review summarizes the currently available software used for prokaryotic genomes TEs annotation (reviewed in  <ref name=":7" />).<br />
*'''Eukaryotic TE nomenclature and naming attribution'''
TEs have been found in virtually all eukaryotic species investigated so far, displaying an extreme diversity, revealed by thousands or even tens of thousands of different TE families<ref name=":2"><pubmed> 2543105</pubmed></nowiki></ref>. Finnegan and colleagues in 1989,  proposed the first TE classification system, which distinguished two classes by their transposition intermediate: RNA ('''Class I or retrotransposons''') or DNA ('''Class II or DNA transposons''')<ref name=":2" /><ref name=":3"><pubmed>2543105</pubmed></nowiki></ref>. The transposition mechanism of '''Class I''' is commonly called ‘'''copy-and-paste'''’, and that of '''Class II''', ‘'''cut-and-paste'''’ <ref name=":3" />. Later, in 2007, Wicker and colleagues proposed a common TE classification system that can be easily handled by non-specialists during genome and TEs annotation procedures<ref name=":8"><pubmed>17984973</pubmed></nowiki></ref>. This system provided a consensus between the various conflicting classification and naming systems that are currently in use<ref name=":2" />. A key component of this system is a naming convention: a three-letter code with each letter respectively denoting '''class''', '''order''' and '''superfamily'''; the '''family''' (or '''subfamily''') name; the sequence (database accession number) on which the element was found; and the ‘running number’, which defines the individual insertion in the accession. The unified system is also intended to facilitate comparative and evolutionary studies on TEs from different species <ref name=":8" /> .
Nowadays, the Wicker TE classification system is well-recognized by the scientific community and is currently in use by the TE annotation pipelines.
<references />

Revision as of 11:06, 21 March 2022





  • Class I Elements: (see also)
  • Class II Elements: (see also)
  • Cre Recombinase: The Cre Recombinase is a DNA breaking and rejoining enzyme (from tyrosine recombinase family) derived from the P1 bacteriophage. It is widely used for conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes. (see also).
  • Co-integrate:
  • Copy-and-paste transposition model:
  • Copy-out–Paste-in transposition model:
  • Cut-and-paste transposition model:


  • Direct Repeats (DRs):
  • DDE Domain:
  • Donor Primed Replicative Transposition (DPRT):




  • Group II introns: The group II introns are mobile retroelements that use the combined activities of an autocatalytic RNA and an intron-encoded reverse transcriptase (RT) to propagate efficiently within genomes. They are intimately related with the evolution of eukaryotes, being ancestrally related to nuclear spliceosomal introns, retrotransposons and telomerase (see also).
  • Genome decay:
  • Genome Rearrangements:


  • Horizontal gene transfer (or Lateral gene transfer):
  • Homologous recombination:


  • Inverted Repeats (IR):
  • Integron: Integrons one class of Mobile Genetic Elements (MGEs) are specific genetic structure composed by genes (named as integron cassettes) that generally allow bacteria to adapt and rapidly evolve through the acquisition, stockpiling, excision, and reordering of open reading frames found the integron cassettes. The integron mobilization is mediated by site-specific recombination reactions by the integrase (see also).
  • Invertases:





  • Mobilome:
  • Methylase (Methylation):




  • Plasmidome:
  • plasmid F:
  • Pseudogenisation:
  • Peel-and-paste (Single-strand) transposition model:
  • Phage tyrosine integrase:
  • Phage serine integrase:



  • Resolution site:
  • Resolvase:
  • Rolling-circle transposition model:


  • Synaptic complex:
  • Serine recombinase: The serine recombinases are a family of DNA breaking and rejoining enzymes. Unlike homologous recombination, the serine recombinases promote rearrangements of DNA by breaking and rejoining strands at precisely defined sequence positions (see also).
  • Serine Resolvases: The serine resolvases and the closely related invertases, are a group of site-specific recombinases that, in their native contexts, resolve large fused replicons into smaller separated ones
  • Single-strand transposition:
  • Site-specific recombination:


  • Target specificity:
  • Transposase:
  • Transposome:
  • Target Primed Replicative Transposition (TPRT):
  • Tyrosine recombinase: Tyrosine site-specific recombinases (YRs) are a family of DNA breaking and rejoining enzymes which use the active site tyrosine nucleophile for DNA strand breakage (see also).



  • V(D)J Recombination:



  • Xer Site-Specific Recombination: