Difference between revisions of "General Information/ IS derivatives of Tn3 family transposons"
Line 1: | Line 1: | ||
− | '''<big>A</big>'''nother source of ambiguity for classification purposes occurs in the Tn''3'' family (see section “[[Transposons families|Tn families]]” and "[[Transposons families/Tn3 family|Tn''3'' family]]") [[:Image:1.15.1.png|(Fig.10.1]] and [[:Image:1.15.1.png|10.2)]]. Tn''3'' family members are quite variable. They include a number of diverse passenger genes that can represent entire operons, notably mercury resistance, or individual genes involved in antibiotic resistance, breakdown of halogenated aromatics, or virulence [e.g. <ref><nowiki><pubmed>10477306</pubmed></nowiki></ref>]]. They often carry integron recombination platforms enabling them to incorporate additional resistance genes by recruiting integron cassettes. Members are quite characteristic: they have long relatively well conserved IR and a particularly long Tpase (950 to 1025 aa). They also encode a site-specific recombination (“resolution”) system necessary for completion of their transposition ([https://www.asmscience.org/content/book/10.1128/9781555817954.chap14 Grindley 2002] - Chapter 14 from [https://www.asmscience.org/content/book/10.1128/9781555817954 Mobile DNA II] book). There are a number of different resolution systems associated with different members of this family [[:Image:1.15.2.png|(Fig.10.2)]] IS''1071'', [[:Image:1.15.1.png|(Fig.10.1)]] composed of Tn''3''-like IR and Tpase gene but lacking both the site-specific recombination system and passenger genes was identified many years ago<ref><nowiki><pubmed>1656436</pubmed></nowiki></ref><ref><nowiki><pubmed>8117095</pubmed></nowiki></ref>. This clearly accords with the definition of an IS. Several other examples have now been identified (e.g. IS''Vsa19'', IS''Shfr9'', IS''Busp1''). | + | '''<big>A</big>'''nother source of ambiguity for classification purposes occurs in the Tn''3'' family (see section “[[Transposons families|Tn families]]” and "[[Transposons families/Tn3 family|Tn''3'' family]]") [[:Image:1.15.1.png|(Fig.10.1]] and [[:Image:1.15.1.png|10.2)]] <ref><nowiki><pubmed>26350313</pubmed></nowiki></ref>. Tn''3'' family members are quite variable. They include a number of diverse passenger genes that can represent entire operons, notably mercury resistance, or individual genes involved in antibiotic resistance, breakdown of halogenated aromatics, or virulence [e.g. <ref><nowiki><pubmed>10477306</pubmed></nowiki></ref>]]. They often carry integron recombination platforms enabling them to incorporate additional resistance genes by recruiting integron cassettes <ref><nowiki><pubmed>16845431</pubmed></nowiki></ref>. Members are quite characteristic: they have long relatively well conserved IR and a particularly long Tpase (950 to 1025 aa). They also encode a site-specific recombination (“resolution”) system necessary for completion of their transposition ([https://www.asmscience.org/content/book/10.1128/9781555817954.chap14 Grindley 2002] - Chapter 14 from [https://www.asmscience.org/content/book/10.1128/9781555817954 Mobile DNA II] book). There are a number of different resolution systems associated with different members of this family [[:Image:1.15.2.png|(Fig.10.2)]] IS''1071'', [[:Image:1.15.1.png|(Fig.10.1)]] composed of Tn''3''-like IR and Tpase gene but lacking both the site-specific recombination system and passenger genes was identified many years ago<ref><nowiki><pubmed>1656436</pubmed></nowiki></ref><ref><nowiki><pubmed>8117095</pubmed></nowiki></ref>. This clearly accords with the definition of an IS. Several other examples have now been identified (e.g. IS''Vsa19'', IS''Shfr9'', IS''Busp1''). |
<center> | <center> | ||
{| | {| |
Revision as of 19:59, 27 June 2020
Another source of ambiguity for classification purposes occurs in the Tn3 family (see section “Tn families” and "Tn3 family") (Fig.10.1 and 10.2) [1]. Tn3 family members are quite variable. They include a number of diverse passenger genes that can represent entire operons, notably mercury resistance, or individual genes involved in antibiotic resistance, breakdown of halogenated aromatics, or virulence [e.g. [2]]]. They often carry integron recombination platforms enabling them to incorporate additional resistance genes by recruiting integron cassettes [3]. Members are quite characteristic: they have long relatively well conserved IR and a particularly long Tpase (950 to 1025 aa). They also encode a site-specific recombination (“resolution”) system necessary for completion of their transposition (Grindley 2002 - Chapter 14 from Mobile DNA II book). There are a number of different resolution systems associated with different members of this family (Fig.10.2) IS1071, (Fig.10.1) composed of Tn3-like IR and Tpase gene but lacking both the site-specific recombination system and passenger genes was identified many years ago[4][5]. This clearly accords with the definition of an IS. Several other examples have now been identified (e.g. ISVsa19, ISShfr9, ISBusp1).