Difference between revisions of "General Information/The casposases"
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==The casposases== | ==The casposases== | ||
| − | More recently, TE related to [[wikipedia:CRISPR|CRISPRs]], Casposons have been identified<ref><nowiki><pubmed>24884953</pubmed></nowiki></ref> and a reassement of their ends has led to the identification of an 14-15 bp target duplication <ref><nowiki><pubmed>PMC4150552</pubmed></nowiki></ref>. Moreover, the purified [[wikipedia:Cas1|Cas1]] enzyme encoded by these ancestral transposons has been demonstrated to catalyse strand transfer of a pre-cleaved transposon ''in vitro'' but does not appear to promote transposon strand cleavage in this assay<ref><nowiki><pubmed>26573596</pubmed></nowiki></ref>. Cas1 "casposases" use similar chemistry to that used by the CRISPR Cas1-Cas2 complex but with opposite substrate specificities since CRISPR Cas1-Cas2 integrates "random" sequences into a specific site in the CRISPR locus whereas casposases integrate specific site (the casposon ends) into random target sequences [[:Image:1.44.png|(Fig.24.1)]]. | + | More recently, TE related to [[wikipedia:CRISPR|CRISPRs]], Casposons have been identified<ref><nowiki><pubmed>24884953</pubmed></nowiki></ref> and a reassement of their ends has led to the identification of an 14-15 bp target duplication <ref><nowiki><pubmed>PMC4150552</pubmed></nowiki></ref>. Moreover, the purified [[wikipedia:Cas1|Cas1]] enzyme encoded by these ancestral transposons has been demonstrated to catalyse strand transfer of a pre-cleaved transposon ''in vitro'' but does not appear to promote transposon strand cleavage in this assay<ref><nowiki><pubmed>26573596</pubmed></nowiki></ref>. [[wikipedia:Cas1|Cas1]] "casposases" use similar chemistry to that used by the CRISPR [[wikipedia:Cas1|Cas1]]-[[wikipedia:Cas2|Cas2]] complex but with opposite substrate specificities since CRISPR [[wikipedia:Cas1|Cas1]]-[[wikipedia:Cas2|Cas2]] integrates "random" sequences into a specific site in the CRISPR locus whereas casposases integrate a specific site (the casposon ends) into random target sequences [[:Image:1.44.png|(Fig.24.1)]]. |
[[Image:1.44.png|thumb|center|510x510px|'''Fig.24.1.''' Organization of the ''[[wikipedia:Aciduliprofundum_boonei|Aciduliprofundum boonei]]'' casposon. The casposon is shown as an unfilled box. Black filled arrow-heads represent the terminal inverted repeats (TIR); green arrows, the target site duplication (TSD); the genes are as follows: type B DNA polymerase (red box); HNH endonuclease (green); transcriptional regulator with HTH domain (pink); hypothetical protein with HTH domain (orange); cas1 (dark blue) with a c-terminal HTH (light blue); N6-methyltransferase (yellow). From Hickman and Dyda (2015). Non-CRISPR-associated cas1 form two distinct clades. One contains three distinct casposon families (1, 2, and 3) which share features with eukaryotic Polinton/Maverick eukaryotic transposons labeled ‘self-synthesizing’ since they include B family DNA polymerase genes. Members of the three families differ in gene content and evolutionary provenance of the DNA polymerases (protein-primed or RNA-primed).|alt=]] | [[Image:1.44.png|thumb|center|510x510px|'''Fig.24.1.''' Organization of the ''[[wikipedia:Aciduliprofundum_boonei|Aciduliprofundum boonei]]'' casposon. The casposon is shown as an unfilled box. Black filled arrow-heads represent the terminal inverted repeats (TIR); green arrows, the target site duplication (TSD); the genes are as follows: type B DNA polymerase (red box); HNH endonuclease (green); transcriptional regulator with HTH domain (pink); hypothetical protein with HTH domain (orange); cas1 (dark blue) with a c-terminal HTH (light blue); N6-methyltransferase (yellow). From Hickman and Dyda (2015). Non-CRISPR-associated cas1 form two distinct clades. One contains three distinct casposon families (1, 2, and 3) which share features with eukaryotic Polinton/Maverick eukaryotic transposons labeled ‘self-synthesizing’ since they include B family DNA polymerase genes. Members of the three families differ in gene content and evolutionary provenance of the DNA polymerases (protein-primed or RNA-primed).|alt=]] | ||
Revision as of 18:04, 9 August 2021
The casposases
More recently, TE related to CRISPRs, Casposons have been identified[1] and a reassement of their ends has led to the identification of an 14-15 bp target duplication [2]. Moreover, the purified Cas1 enzyme encoded by these ancestral transposons has been demonstrated to catalyse strand transfer of a pre-cleaved transposon in vitro but does not appear to promote transposon strand cleavage in this assay[3]. Cas1 "casposases" use similar chemistry to that used by the CRISPR Cas1-Cas2 complex but with opposite substrate specificities since CRISPR Cas1-Cas2 integrates "random" sequences into a specific site in the CRISPR locus whereas casposases integrate a specific site (the casposon ends) into random target sequences (Fig.24.1).
Fig.24.1. Organization of the Aciduliprofundum boonei casposon. The casposon is shown as an unfilled box. Black filled arrow-heads represent the terminal inverted repeats (TIR); green arrows, the target site duplication (TSD); the genes are as follows: type B DNA polymerase (red box); HNH endonuclease (green); transcriptional regulator with HTH domain (pink); hypothetical protein with HTH domain (orange); cas1 (dark blue) with a c-terminal HTH (light blue); N6-methyltransferase (yellow). From Hickman and Dyda (2015). Non-CRISPR-associated cas1 form two distinct clades. One contains three distinct casposon families (1, 2, and 3) which share features with eukaryotic Polinton/Maverick eukaryotic transposons labeled ‘self-synthesizing’ since they include B family DNA polymerase genes. Members of the three families differ in gene content and evolutionary provenance of the DNA polymerases (protein-primed or RNA-primed).
