Difference between revisions of "General Information/IS Distribution"

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Revision as of 13:46, 4 December 2022

ISs are widespread (Fig.6.1) and can occur in very high numbers in prokaryotic genomes. A recent study concluded that proteins annotated as Tpases, or as proteins with related functions, are by far the most abundant functional class in both the prokaryotic and eukaryotic genomic and metagenomic public databases[1] (Fig.2.5).

Fig.6.1. IS distribution in the prokaryotic world.

Since the last surveys (e.g. [2][3]) many new ISs have been identified largely as a result of the massive increase in available sequenced prokaryotic genomes. Careful analysis of a number of these has also revealed that some genomes contain significant levels of truncated and partial ISs devoid of Tpase genes. These genomic "scars" represent traces of numerous ancestral transposition events. However, genome annotations are often based simply on the presence of Tpase genes (e.g. [4]) and do not include the entire DNA sequence with the IS ends. Indeed, a significant number of solo IS-related Inverted Repeats (IRs) have been identified in various genomes. Small IS fragments are rarely taken into account even though they can provide important insights into the evolutionary history of the host genome (Fig.6.2), (Fig.6.3) and (Fig.6.4). Not only can this seriously impair studies attempting to provide an overview of the evolutionary influence of TEs on bacterial and archeal genomes, but such fragments may encode truncated proteins and these could influence gene regulation (e.g. [5][6][7]). In bacteria [8][9][10][11] and eukaryotes[12][13] truncated transposases have been shown to inhibit transposition. One example where annotation of IS fragments has provided important information is in the obligatory intracellular insect endosymbiont, Wolbachia, which also carries high numbers of full-length ISs. The sequence divergence observed suggests that several waves of IS invasion and elimination have occurred over evolutionary time [14].

Fig.6.2. Shigella an IS jungle. A Southern blot of EcoRI-digested genomic DNA of various Shigella species and hybridization using radioactively labeled probes of insertion sequences IS1, IS2 (IS3 family), IS15 (IS6 family) and IS911 (IS3 family).
Fig.6.3. The Shigella flexneri virulence plasmid circular map. The outer circle indicates (in green) both full length IS of different types and IS fragments, scars of previous transposition and recombination events, and IS decay. The second circle shows the location of complete IS copies. The inner-circle shows plasmid coordinates.


Fig.6.4. The Sulfolobus solfataricus P2 circular genome representation showing Complete IS (blue) and IS fragments (grey/brown). The IS family to which each IS belongs is shown in brackets. For a more detailed visualization, please go to ISbrowser Graphical Display of S. solfataricus P2 genome.

Bibliography

  1. ↑ Aziz RK, Breitbart M, Edwards RA . Transposases are the most abundant, most ubiquitous genes in nature. - Nucleic Acids Res: 2010 Jul, 38(13);4207-17 [PubMed:20215432] [DOI]
  2. ↑ Chandler M, Mahillon J. Insertion Sequences Revisited. In: Craig NL, Lambowitz AM, Craigie R, Gellert M, editors. Mobile DNA II. American Society of Microbiology; 2002. p. 305–366.
  3. ↑ Mahillon J, Chandler M . Insertion sequences. - Microbiol Mol Biol Rev: 1998 Sep, 62(3);725-74 [PubMed:9729608] [DOI]
  4. ↑ Touchon M, Rocha EP . Causes of insertion sequences abundance in prokaryotic genomes. - Mol Biol Evol: 2007 Apr, 24(4);969-81 [PubMed:17251179] [DOI]
  5. ↑ Cordaux R, Udit S, Batzer MA, Feschotte C . Birth of a chimeric primate gene by capture of the transposase gene from a mobile element. - Proc Natl Acad Sci U S A: 2006 May 23, 103(21);8101-6 [PubMed:16672366] [DOI]
  6. ↑ Shaheen M, Williamson E, Nickoloff J, Lee SH, Hromas R . Metnase/SETMAR: a domesticated primate transposase that enhances DNA repair, replication, and decatenation. - Genetica: 2010 May, 138(5);559-66 [PubMed:20309721] [DOI]
  7. ↑ Wray J, Williamson EA, Royce M, Shaheen M, Beck BD, Lee SH, Nickoloff JA, Hromas R . Metnase mediates resistance to topoisomerase II inhibitors in breast cancer cells. - PLoS One: 2009, 4(4);e5323 [PubMed:19390626] [DOI]
  8. ↑ Stalder R, Caspers P, Olasz F, Arber W . The N-terminal domain of the insertion sequence 30 transposase interacts specifically with the terminal inverted repeats of the element. - J Biol Chem: 1990 Mar 5, 265(7);3757-62 [PubMed:2154486]
  9. ↑ Salvatore P, Pagliarulo C, Colicchio R, Zecca P, Cantalupo G, Tredici M, Lavitola A, Bucci C, Bruni CB, Alifano P . Identification, characterization, and variable expression of a naturally occurring inhibitor protein of IS1106 transposase in clinical isolates of Neisseria meningitidis. - Infect Immun: 2001 Dec, 69(12);7425-36 [PubMed:11705917] [DOI]
  10. ↑ Gueguen E, Rousseau P, Duval-Valentin G, Chandler M . Truncated forms of IS911 transposase downregulate transposition. - Mol Microbiol: 2006 Nov, 62(4);1102-16 [PubMed:17078817] [DOI]
  11. ↑ de la Cruz NB, Weinreich MD, Wiegand TW, Krebs MP, Reznikoff WS . Characterization of the Tn5 transposase and inhibitor proteins: a model for the inhibition of transposition. - J Bacteriol: 1993 Nov, 175(21);6932-8 [PubMed:8226636] [DOI]
  12. ↑ Rio DC . Regulation of Drosophila P element transposition. - Trends Genet: 1991 Sep, 7(9);282-7 [PubMed:1662417] [DOI]
  13. ↑ Vos JC, van Luenen HG, Plasterk RH . Characterization of the Caenorhabditis elegans Tc1 transposase in vivo and in vitro. - Genes Dev: 1993 Jul, 7(7A);1244-53 [PubMed:8391505] [DOI]
  14. ↑ Cerveau N, Leclercq S, Leroy E, Bouchon D, Cordaux R . Short- and long-term evolutionary dynamics of bacterial insertion sequences: insights from Wolbachia endosymbionts. - Genome Biol Evol: 2011, 3;1175-86 [PubMed:21940637] [DOI]


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