ISWz1
- Family IS91
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| WZU14952 | ND | Weeksella zoohelcum | Weeksella zoohelcum |
DNA section
IS Length : 2270 bp
Ends
oriIS : TTCCGGGATTTGCGGAGCTTTTGCATAGGTTTGAACGTACGGTTTCTGTGTTGGGAAGAAGCCAAAGTACCTTTCAAAATTACTCCAGACACGTCGCTGC II struct. : No
terIS : CGCCCACAAAAAAAGAGACTCTTCTCAAAGTCTCTTGTATCTCTAAAAATCTTTTGTCGATAAACCCATAGTGTGGGAAAATACCCAACCAAAAGCTCCC II struct. : No
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| TCAATCAGGCAAAGCGC GAAG | GAAG CTTCCGTTCAACAGG |
DNA sequence
TTCCGGGATTTGCGGAGCTTTTGCATAGGTTTGAACGTACGGTTTCTGTGTTGGGAAGAAGCCAAAGTACCTTTCAAAATTACTCCAGACACGTCGCTGC
GGTGTCGCTACATTTCGGGAAAATTCCTACAGAATTGGATCCCGAGCAAATTCACGATTACCTTTTTTATCTACAGAAAAAATCTAAATCACCTTCACAG
TCGTACTTTAAACATACCGTTTACGGGCTTCGGTTTCTGCTAAAATCGGAAGGTTTAAGCTATGATTTTTTGAGTCTTCCGGAAATTAAAAGAGAGAAAA
AACTGCCTGTAGTTCTCAGTAAACAGGAGGTTTGGCAGATGCTTTCGGGGTGTAAGCTTTTAAAGCATAAAATCTTAATCGGGATTCTTTACGGTTGCGG
ATTGCGATGTATGGAAGTTCGAAATCTGCGTTTGTGCGACTTAGATTTTGACAGAAAACAACTCAAAGTGGTTCAGGGAAAAGGAAAAAAAGACCGTTAT
TTGCCACTTTCCGAGCATTTGATTCGGGGACTCAAAAAGTATATCGAAGCGGAAAAACCGGAAGATTATCTCTTTGGAGAACCTCGTGGAAACCGTGCCG
GCGGAGAGTTTGATTCCCGCTACTCACAACGAGGCGTTCAGTGGGCGGTAAAACAGGCATCAAAAACGGCAAACATCCTGAAAGAAGTGAGTGTCCATAC
GCTTCGTCACAGTTTTGCGACGCATCTTCTGGAAGATGGGATGGATATTTTGAGCATCAAAAATCTCCTCGGCCACGAAAGTATTGATACGACGTTGATT
TATCTCCAAATTGCACAACTTTCCACACAAAAACTCTTTTCACCGCTCGATACCCTTTTTTCAGAATTTGGGAAAAAATGAGCGGTTTAAAAACCTCAAA
AAAACAGTCAGTTCAACCTCAATCTCAACCTCAATACGAAGTCGCCGATGTTTTAAATATTTTAGGTTCAAAATTGGAAAATTTAGAACTTAATTCTTGG
CAGTTAAGAACTTTATTTGCCTTAAAAAAGTGCAGAACTTCGGCTTTGGGCGGTCATATCGATGCTTGTGATGAATGTGGAAATGTAAGCATCAGTTACA
ACTCCTGCCGAAACCGTCACTGCCCAAAATGTCAGGGAAAAAAACGAGAACAATGGATTGAGAATCGGGAAACCGAACTGCTTCCGGTACCTTATTTTAC
TCATTTTTTATTATTCGTTTTAATTGTATTCGTGATACGCTTCGCTTAATTCACGTGGTTTTTACGCTTCCTGATGTATTGAACAAAACCGCACTTCACG
AGCCCAAAATGTTGTACGATTTTTTATTTGAATCTGCCTGGGAAACGCTTCAAACGTTTGGTGAAAATCGAGGTTTAAAAATGGGAATGATTGCTGTTTT
GCATACTTGGGGACAGAATCTGAGCCTTCATCCGCATTTGCACTGCATTGTTCCGGGCGGTGGAGTAGATGAAAGCGGACGTTGGAAAAATCTACGTTCA
GACGGCAAATTTTTGTTTCCGGTAAAGGCTTTGAGTAAAGTTTTCAGGGCTAAATTTTGTGAGAAATTAAAAGATAAAAACCTTAAAGAATATACCAAAA
TCAGGCAAAATCTGTGGGAAAAACCTTGGGTGGTTTACGCCAAAAAGCCTTTTGGAAGCCCAAAATCTGTGGTAGAATATTTGGGCAGATACACGCATAA
AATCGCCATCAGCAACCAGAGAATCAGGAAAATTGACGCGAAAACGGTCACTTTTTCTTACAAAGATTACCGCCAAAAAGGTATCAAAAAGCAGATGGTT
TTGAGCCACGAGGAGTTTATCCGAAGATTTGCGATGCATATTTTACCGAAAAGATTTGTAAAAATCCGTCATTATGGTTTTTTGAGCAGCACTTGGAAGC
GTATCAAGCTTAAAAATCTACAACAAAAATTAGGCATCCAGCCTAAAGAAAACGTTCCGCCAAAAGTTTTTCAGCCGAAATGTAGTTGTTGTAAAGTTGG
AAATTTGGTAACGATTGCCACGTTCGATCTTCGAGGTCCGCCGCAATGGTTTTTGGAGATGAGCCAAAATTTATCTGCTCCTAAATCTGCATTTTAGCGG
ATTTGGGTAAGGGATTTTATGTCTAAACGTGAAAGAAAACACAATAAAACCAAGAAAAAACCACCAAAATCGCCCACAAAAAAAGAGACTCTTCTCAAAG
TCTCTTGTATCTCTAAAAATCTTTTGTCGATAAACCCATAGTGTGGGAAAATACCCAACCAAAAGCTCCC
GGTGTCGCTACATTTCGGGAAAATTCCTACAGAATTGGATCCCGAGCAAATTCACGATTACCTTTTTTATCTACAGAAAAAATCTAAATCACCTTCACAG
TCGTACTTTAAACATACCGTTTACGGGCTTCGGTTTCTGCTAAAATCGGAAGGTTTAAGCTATGATTTTTTGAGTCTTCCGGAAATTAAAAGAGAGAAAA
AACTGCCTGTAGTTCTCAGTAAACAGGAGGTTTGGCAGATGCTTTCGGGGTGTAAGCTTTTAAAGCATAAAATCTTAATCGGGATTCTTTACGGTTGCGG
ATTGCGATGTATGGAAGTTCGAAATCTGCGTTTGTGCGACTTAGATTTTGACAGAAAACAACTCAAAGTGGTTCAGGGAAAAGGAAAAAAAGACCGTTAT
TTGCCACTTTCCGAGCATTTGATTCGGGGACTCAAAAAGTATATCGAAGCGGAAAAACCGGAAGATTATCTCTTTGGAGAACCTCGTGGAAACCGTGCCG
GCGGAGAGTTTGATTCCCGCTACTCACAACGAGGCGTTCAGTGGGCGGTAAAACAGGCATCAAAAACGGCAAACATCCTGAAAGAAGTGAGTGTCCATAC
GCTTCGTCACAGTTTTGCGACGCATCTTCTGGAAGATGGGATGGATATTTTGAGCATCAAAAATCTCCTCGGCCACGAAAGTATTGATACGACGTTGATT
TATCTCCAAATTGCACAACTTTCCACACAAAAACTCTTTTCACCGCTCGATACCCTTTTTTCAGAATTTGGGAAAAAATGAGCGGTTTAAAAACCTCAAA
AAAACAGTCAGTTCAACCTCAATCTCAACCTCAATACGAAGTCGCCGATGTTTTAAATATTTTAGGTTCAAAATTGGAAAATTTAGAACTTAATTCTTGG
CAGTTAAGAACTTTATTTGCCTTAAAAAAGTGCAGAACTTCGGCTTTGGGCGGTCATATCGATGCTTGTGATGAATGTGGAAATGTAAGCATCAGTTACA
ACTCCTGCCGAAACCGTCACTGCCCAAAATGTCAGGGAAAAAAACGAGAACAATGGATTGAGAATCGGGAAACCGAACTGCTTCCGGTACCTTATTTTAC
TCATTTTTTATTATTCGTTTTAATTGTATTCGTGATACGCTTCGCTTAATTCACGTGGTTTTTACGCTTCCTGATGTATTGAACAAAACCGCACTTCACG
AGCCCAAAATGTTGTACGATTTTTTATTTGAATCTGCCTGGGAAACGCTTCAAACGTTTGGTGAAAATCGAGGTTTAAAAATGGGAATGATTGCTGTTTT
GCATACTTGGGGACAGAATCTGAGCCTTCATCCGCATTTGCACTGCATTGTTCCGGGCGGTGGAGTAGATGAAAGCGGACGTTGGAAAAATCTACGTTCA
GACGGCAAATTTTTGTTTCCGGTAAAGGCTTTGAGTAAAGTTTTCAGGGCTAAATTTTGTGAGAAATTAAAAGATAAAAACCTTAAAGAATATACCAAAA
TCAGGCAAAATCTGTGGGAAAAACCTTGGGTGGTTTACGCCAAAAAGCCTTTTGGAAGCCCAAAATCTGTGGTAGAATATTTGGGCAGATACACGCATAA
AATCGCCATCAGCAACCAGAGAATCAGGAAAATTGACGCGAAAACGGTCACTTTTTCTTACAAAGATTACCGCCAAAAAGGTATCAAAAAGCAGATGGTT
TTGAGCCACGAGGAGTTTATCCGAAGATTTGCGATGCATATTTTACCGAAAAGATTTGTAAAAATCCGTCATTATGGTTTTTTGAGCAGCACTTGGAAGC
GTATCAAGCTTAAAAATCTACAACAAAAATTAGGCATCCAGCCTAAAGAAAACGTTCCGCCAAAAGTTTTTCAGCCGAAATGTAGTTGTTGTAAAGTTGG
AAATTTGGTAACGATTGCCACGTTCGATCTTCGAGGTCCGCCGCAATGGTTTTTGGAGATGAGCCAAAATTTATCTGCTCCTAAATCTGCATTTTAGCGG
ATTTGGGTAAGGGATTTTATGTCTAAACGTGAAAGAAAACACAATAAAACCAAGAAAAAACCACCAAAATCGCCCACAAAAAAAGAGACTCTTCTCAAAG
TCTCTTGTATCTCTAAAAATCTTTTGTCGATAAACCCATAGTGTGGGAAAATACCCAACCAAAAGCTCCC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 780 bp | 259 aa | 102 | 881 | + | No |
AG : IS91 integrase/resolvase
ORF sequence :
MSLHFGKIPTELDPEQIHDYLFYLQKKSKSPSQSYFKHTVYGLRFLLKSEGLSYDFLSLPEIKREKKLPVVLSKQEVWQMLSGCKLLKHKILIGILYGCG
LRCMEVRNLRLCDLDFDRKQLKVVQGKGKKDRYLPLSEHLIRGLKKYIEAEKPEDYLFGEPRGNRAGGEFDSRYSQRGVQWAVKQASKTANILKEVSVHT
LRHSFATHLLEDGMDILSIKNLLGHESIDTTLIYLQIAQLSTQKLFSPLDTLFSEFGKK
LRCMEVRNLRLCDLDFDRKQLKVVQGKGKKDRYLPLSEHLIRGLKKYIEAEKPEDYLFGEPRGNRAGGEFDSRYSQRGVQWAVKQASKTANILKEVSVHT
LRHSFATHLLEDGMDILSIKNLLGHESIDTTLIYLQIAQLSTQKLFSPLDTLFSEFGKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 388 aa | 878 | 2097 | + | No |
Chemistry : Y2
ORF sequence :
MSGLKTSKKQSVQPQSQPQYEVADVLNILGSKLENLELNSWQLRTLFALKKCRTSALGGHIDACDECGNVSISYNSCRNRHCPKCQGKKREQWIENRETE
LLPVPYFHVVFTLPDVLNKTALHEPKMLYDFLFESAWETLQTFGENRGLKMGMIAVLHTWGQNLSLHPHLHCIVPGGGVDESGRWKNLRSDGKFLFPVKA
LSKVFRAKFCEKLKDKNLKEYTKIRQNLWEKPWVVYAKKPFGSPKSVVEYLGRYTHKIAISNQRIRKIDAKTVTFSYKDYRQKGIKKQMVLSHEEFIRRF
AMHILPKRFVKIRHYGFLSSTWKRIKLKNLQQKLGIQPKENVPPKVFQPKCSCCKVGNLVTIATFDLRGPPQWFLEMSQNLSAPKSAF
LLPVPYFHVVFTLPDVLNKTALHEPKMLYDFLFESAWETLQTFGENRGLKMGMIAVLHTWGQNLSLHPHLHCIVPGGGVDESGRWKNLRSDGKFLFPVKA
LSKVFRAKFCEKLKDKNLKEYTKIRQNLWEKPWVVYAKKPFGSPKSVVEYLGRYTHKIAISNQRIRKIDAKTVTFSYKDYRQKGIKKQMVLSHEEFIRRF
AMHILPKRFVKIRHYGFLSSTWKRIKLKNLQQKLGIQPKENVPPKVFQPKCSCCKVGNLVTIATFDLRGPPQWFLEMSQNLSAPKSAF
Blast result :
Comments
ISWz1 has been detected by Brassard et al. (1994) downstream the AccI restriction and methylase genes in Weeksella zoohelcum. This 1219 region shares homology with members of the IS91 family.
Moreover, its close structural association with a downstream integrase gene remains to be detailed. Finally, the authors have also reported a 53-bp insertion in the middle of ISWz1 major ORF: consequently, the 388-aa ORF considered here results from the fusion between a 107- and a 281-aa ORF. Note: Weeksella zoohelcum is commensal of the internal surfaces of humans and other warm-blooded animals. ISWz1 is adjacent to the AccI restriction-modification operon.
ISWz1 is 54% (ORFA : integrase) and 56% (ORFB: transposase) aa similar to ISAzo26.
Moreover, its close structural association with a downstream integrase gene remains to be detailed. Finally, the authors have also reported a 53-bp insertion in the middle of ISWz1 major ORF: consequently, the 388-aa ORF considered here results from the fusion between a 107- and a 281-aa ORF. Note: Weeksella zoohelcum is commensal of the internal surfaces of humans and other warm-blooded animals. ISWz1 is adjacent to the AccI restriction-modification operon.
ISWz1 is 54% (ORFA : integrase) and 56% (ORFB: transposase) aa similar to ISAzo26.
References
1] Brassard, S., Paquet, H., and Roy, P.H. (1994) EMBL Data Library, Rel. 41.