ISVvu1
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AP005332 | ND | Vibrio vulnificus | Vibrio vulnificus |
DNA section
IS Length : 793 bp
Ends
IR Length : 13/14
IRL : GGTCGTGACTCCAACATGGAGATGTTGTGTTAAAATGGTAGCCTTTTCAC
IRR : GGTCATGACTCCAATCGGTTGATTTAAAAATAGAATTCCCGTTCAATGAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGTCGTGACTCCAACATGGAGATGTTGTGTTAAAATGGTAGCCTTTTCACCCTCAAATTAGAAATCATGGCTACCATTCAAGCTCAATGTCGATTTTGCA
ACCAAACTGAACATGTTCGTAAACACGGAACTGGAGTAACAGGCTTTCAGCGCTTTCGTTGCATTGAGTGCAAGCGTTCTTTTCAGCTTGATTATGTATA
TGAGGCATATAAGCCTAATGTCAAAAAACAAATCATTGATATGGCAATGAATAGCTCTGGTGTAAGAGAAACAAGCCGTGTCTTAAAAGTTGGCTATAAC
ACAGTGCTACGCACTTTAAAAAACTCTCGCCAAGGCAAGTGACCTCGATTCCATTCGATAAAGCAAATATCTCTCTCATCTGTGAAGTTGATGAGCAATG
GTCATTCGTCGGTAAAAAGAAAAATCGCCGCTGGCTTTGGTATGCTTGGGAGCCTCGCTATAAACGCATCATTGCTCACGCATTTGGTAAACGAAATACG
GATGCTCTTCGATCGTTACTCACTTTGCTAAAGCCGTTCGACATCAGCTTCTTTTTTACCGATGACTTCAGCGTTTACGCAAAAGAATTACCAAAGGAAA
AGCATGTTGTAGGCAAAAGGTTCACTCAGCGGATTGAGCGAACCAATCTGACCTTACGTTCCAGATTAAAACGTTTAGTGCGTAGAACTATCGGCTTTTC
TAGATCGGAGGAGATGCACGATAAGGTGATCGGAACATTCATTGAACGGGAATTCTATTTTTAAATCAACCGATTGGAGTCATGACC
ACCAAACTGAACATGTTCGTAAACACGGAACTGGAGTAACAGGCTTTCAGCGCTTTCGTTGCATTGAGTGCAAGCGTTCTTTTCAGCTTGATTATGTATA
TGAGGCATATAAGCCTAATGTCAAAAAACAAATCATTGATATGGCAATGAATAGCTCTGGTGTAAGAGAAACAAGCCGTGTCTTAAAAGTTGGCTATAAC
ACAGTGCTACGCACTTTAAAAAACTCTCGCCAAGGCAAGTGACCTCGATTCCATTCGATAAAGCAAATATCTCTCTCATCTGTGAAGTTGATGAGCAATG
GTCATTCGTCGGTAAAAAGAAAAATCGCCGCTGGCTTTGGTATGCTTGGGAGCCTCGCTATAAACGCATCATTGCTCACGCATTTGGTAAACGAAATACG
GATGCTCTTCGATCGTTACTCACTTTGCTAAAGCCGTTCGACATCAGCTTCTTTTTTACCGATGACTTCAGCGTTTACGCAAAAGAATTACCAAAGGAAA
AGCATGTTGTAGGCAAAAGGTTCACTCAGCGGATTGAGCGAACCAATCTGACCTTACGTTCCAGATTAAAACGTTTAGTGCGTAGAACTATCGGCTTTTC
TAGATCGGAGGAGATGCACGATAAGGTGATCGGAACATTCATTGAACGGGAATTCTATTTTTAAATCAACCGATTGGAGTCATGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 276 bp | 91 aa | 67 | 342 | + | No |
Description : First part of the transposase
ORF sequence :
MATIQAQCRFCNQTEHVRKHGTGVTGFQRFRCIECKRSFQLDYVYEAYKPNVKKQIIDMAMNSSGVRETSRVLKVGYNTVLRTLKNSRQGK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 465 bp | 154 aa | 300 | 764 | + | No |
Description : Second part of the transposase
ORF sequence :
HSATHFKKLSPRQVTSIPFDKANISLICEVDEQWSFVGKKKNRRWLWYAWEPRYKRIIAHAFGKRNTDALRSLLTLLKPFDISFFFTDDFSVYAKELPKE
KHVVGKRFTQRIERTNLTLRSRLKRLVRRTIGFSRSEEMHDKVIGTFIEREFYF
KHVVGKRFTQRIERTNLTLRSRLKRLVRRTIGFSRSEEMHDKVIGTFIEREFYF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 698 bp | 221 aa | 67 | 764 | + | Yes |
Chemistry : DDE
ORF sequence :
MATIQAQCRFCNQTEHVRKHGTGVTGFQRFRCIECKRSFQLDYVYEAYKPNVKKQIIDMAMNSSGVRETSRVLKKLSPRQVTSIPFDKANISLICEVDEQ
WSFVGKKKNRRWLWYAWEPRYKRIIAHAFGKRNTDALRSLLTLLKPFDISFFFTDDFSVYAKELPKEKHVVGKRFTQRIERTNLTLRSRLKRLVRRTIGF
SRSEEMHDKVIGTFIEREFYF
WSFVGKKKNRRWLWYAWEPRYKRIIAHAFGKRNTDALRSLLTLLKPFDISFFFTDDFSVYAKELPKEKHVVGKRFTQRIERTNLTLRSRLKRLVRRTIGF
SRSEEMHDKVIGTFIEREFYF
Blast result :
Comments
ISVvu1 is 75% (ORf1) and 78% (ORF2) aa similar to ISEhe5.
The third ORF is the putaive ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putaive ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chen,C.Y., Wu,K.M., Chang,Y.C., Chang,C.H., Tsai,H.C., Liao,T.L., Liu,Y.M., Chen,H.J., Shen,A.B., Li,J.C., Su,T.L., Shao,C.P., Lee,C.T., Hor,L.I. and Tsai,S.F.(2003) Genome Res. 13 (12), 2577-2587