ISSpo8
- Family IS1595
- Group ISNwi1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003911 | ND | Silicibacter pomeroyi | Silicibacter pomeroyi DSS-3 |
DNA section
IS Length : 1863 bp
Ends
IR Length : 21/22
IRL : CCCAACTATCCACGAAACAAAGCGTATCCGATTCCATGGTGTTGAAAACC
IRR : CCCAATTATCCACGAAAAAAAGCAAAGCAGTAGAGAGCCTTCTGAAACTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCATTCATCAGGAATG | AAAACTTT | CTGCCCGAAGCGGCAC | 8 |
DNA sequence
CCCAACTATCCACGAAACAAAGCGTATCCGATTCCATGGTGTTGAAAACCATATAGAAAACACACTTGCTCCCCTAATTCGCGCGATTTGAGGCGTGTCT
CTTTGAAATTCGCGGTTTCGGCCAAGATTGCCGATCCACGGAGTTGGAGACAAGTATGATCACAGAATTGAAGTCGGTGCGAGATATCTGGAAGAAGATG
CCCAGCGAGCGCCACGCACGCATTTTCCTTGAAGAGATGATCTGGCCGTCTGGTCGCCATTGCCCACATTGTGGCAGCCTTAGCTCAACCCCAATCCGAG
GGCGCACTGCTCGCCCTGGCCTCTACCAATGCGCCGAACGCGAGTGCTGCCTCCAATTCACGGTGACCACAAAGACCCCGATGCATGCCACGAAACTGGA
CCTTCGGATCTGGATTGCAGCAATCTTTCTGATGCTGACGTCCAGCAAGGGAATCTCGTCTGTGGTGATGGCGCGGATCCTGGGTGTGAATCAGAAAACG
GCCTGGAAACTGGGGCATGCGATCCGGGAACTGATGGATGAACGTGAGGCTGTTTCCGCAAGGTTGGCCGGCGTTGTCGAAGTTGATGAGGCATATGTTG
GTGGCGCGCCGAAGTTCAAGAAAGGCGTGAAGAACAAGCGGGGTCGAGGCACAGGCAAGCCCATGGTTCTGGTCGCGGCGGATCGCACCGGGCAGGCAAA
GGCGACTCTGATCCCGAATGCGAAGGGCGCGACGCTCGGACCGATCATGAAAGAATGGATTGATCCCTCATCGGCCCTCATGACTGACAGCAACACCGCT
TATCGGAAGATCGGTCGGAGCTTTGCATCACACCACACTGTTAACCATGGCAAACGACAGCATTCGCGGCCTTCCAAAGGAGCGCATATCAACACCGTGG
AGGCTGTGAACTCCCAAGTGCAGCGGGCGTTGATTGGTGTCTATCACCGCTTGGGGCGGCAACATCTTCAAAGATATCTGGACGAGATCCTGTGGCAATG
GAACCATCGTCAGCAGGAGGTGAAGATCAGGAACCGAAAAACATCATCTGGCACCAAGACAATTGCTACGAGGGTCTGGAAACCCATCCCTGTCGTTGAT
CAGATGCGCGGTCTTCTTTGCTGCGCCGTAGGGCGACAGTTGCGGCGAACACCTCAATGGGGTTTATGTTGGCCAATACACCTTTTTGAGCACAAAGGAA
CCTTTGCTTGATTCCCTTTCCGTTGGCTCTTTCGCTGGTTCCAATTTTCATTGGGTGTGCCCTGCTCCTTTTGACACGAAAATCAACCTATCAGAGACTT
CACGAACATGCTCGGCGGATCAACCCGATTTATCGCTGGCCCTTTCCGAAGTCAGAACAGAAGCAACTACGTTTCGTAGATTTCAACAGGCTATTCTTGA
GAGGAGTAGGTTATGCTGCCCTCTCCGTAGGAGCACTCTTTTTTTGCGGGTATTTGGTCACTGTTGTCGATCCTTTCCGGGGAGACCGGTTTGACGCTCA
GGCATGGGCTGAAGCCGGAAGTTGTAGAGGACTGAGTGACTGGGAATGCGTAGAGAAAGAGAGCACTTGTCCACGCGGAAGTATGGTGCGTGATCTAACC
AACAACCACTTGCTGCCACAAACGACCCGCCGCGAAGAAGTAATAGAGCTCCTTGGCCGGTCGACATCCAACGTGCAGATCAATGGTTTGGAGTGCCCAG
CATACAGCCTTGGCATGTGCAGTGGTATCAGGATTGACTACGACTCGCTCTATGTGTGCTTTAATGAAGATGGCACCGTCGCATCTGCGGGACATGTGCA
GCATTGAGCATCAGAGTTTCAGAAGGCTCTCTACTGCTTTGCTTTTTTTCGTGGATAATTGGG
CTTTGAAATTCGCGGTTTCGGCCAAGATTGCCGATCCACGGAGTTGGAGACAAGTATGATCACAGAATTGAAGTCGGTGCGAGATATCTGGAAGAAGATG
CCCAGCGAGCGCCACGCACGCATTTTCCTTGAAGAGATGATCTGGCCGTCTGGTCGCCATTGCCCACATTGTGGCAGCCTTAGCTCAACCCCAATCCGAG
GGCGCACTGCTCGCCCTGGCCTCTACCAATGCGCCGAACGCGAGTGCTGCCTCCAATTCACGGTGACCACAAAGACCCCGATGCATGCCACGAAACTGGA
CCTTCGGATCTGGATTGCAGCAATCTTTCTGATGCTGACGTCCAGCAAGGGAATCTCGTCTGTGGTGATGGCGCGGATCCTGGGTGTGAATCAGAAAACG
GCCTGGAAACTGGGGCATGCGATCCGGGAACTGATGGATGAACGTGAGGCTGTTTCCGCAAGGTTGGCCGGCGTTGTCGAAGTTGATGAGGCATATGTTG
GTGGCGCGCCGAAGTTCAAGAAAGGCGTGAAGAACAAGCGGGGTCGAGGCACAGGCAAGCCCATGGTTCTGGTCGCGGCGGATCGCACCGGGCAGGCAAA
GGCGACTCTGATCCCGAATGCGAAGGGCGCGACGCTCGGACCGATCATGAAAGAATGGATTGATCCCTCATCGGCCCTCATGACTGACAGCAACACCGCT
TATCGGAAGATCGGTCGGAGCTTTGCATCACACCACACTGTTAACCATGGCAAACGACAGCATTCGCGGCCTTCCAAAGGAGCGCATATCAACACCGTGG
AGGCTGTGAACTCCCAAGTGCAGCGGGCGTTGATTGGTGTCTATCACCGCTTGGGGCGGCAACATCTTCAAAGATATCTGGACGAGATCCTGTGGCAATG
GAACCATCGTCAGCAGGAGGTGAAGATCAGGAACCGAAAAACATCATCTGGCACCAAGACAATTGCTACGAGGGTCTGGAAACCCATCCCTGTCGTTGAT
CAGATGCGCGGTCTTCTTTGCTGCGCCGTAGGGCGACAGTTGCGGCGAACACCTCAATGGGGTTTATGTTGGCCAATACACCTTTTTGAGCACAAAGGAA
CCTTTGCTTGATTCCCTTTCCGTTGGCTCTTTCGCTGGTTCCAATTTTCATTGGGTGTGCCCTGCTCCTTTTGACACGAAAATCAACCTATCAGAGACTT
CACGAACATGCTCGGCGGATCAACCCGATTTATCGCTGGCCCTTTCCGAAGTCAGAACAGAAGCAACTACGTTTCGTAGATTTCAACAGGCTATTCTTGA
GAGGAGTAGGTTATGCTGCCCTCTCCGTAGGAGCACTCTTTTTTTGCGGGTATTTGGTCACTGTTGTCGATCCTTTCCGGGGAGACCGGTTTGACGCTCA
GGCATGGGCTGAAGCCGGAAGTTGTAGAGGACTGAGTGACTGGGAATGCGTAGAGAAAGAGAGCACTTGTCCACGCGGAAGTATGGTGCGTGATCTAACC
AACAACCACTTGCTGCCACAAACGACCCGCCGCGAAGAAGTAATAGAGCTCCTTGGCCGGTCGACATCCAACGTGCAGATCAATGGTTTGGAGTGCCCAG
CATACAGCCTTGGCATGTGCAGTGGTATCAGGATTGACTACGACTCGCTCTATGTGTGCTTTAATGAAGATGGCACCGTCGCATCTGCGGGACATGTGCA
GCATTGAGCATCAGAGTTTCAGAAGGCTCTCTACTGCTTTGCTTTTTTTCGTGGATAATTGGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1068 bp | 355 aa | 144 | 1211 | + | No |
Chemistry : DDE
ORF sequence :
METSMITELKSVRDIWKKMPSERHARIFLEEMIWPSGRHCPHCGSLSSTPIRGRTARPGLYQCAERECCLQFTVTTKTPMHATKLDLRIWIAAIFLMLTS
SKGISSVVMARILGVNQKTAWKLGHAIRELMDEREAVSARLAGVVEVDEAYVGGAPKFKKGVKNKRGRGTGKPMVLVAADRTGQAKATLIPNAKGATLGP
IMKEWIDPSSALMTDSNTAYRKIGRSFASHHTVNHGKRQHSRPSKGAHINTVEAVNSQVQRALIGVYHRLGRQHLQRYLDEILWQWNHRQQEVKIRNRKT
SSGTKTIATRVWKPIPVVDQMRGLLCCAVGRQLRRTPQWGLCWPIHLFEHKGTFA
SKGISSVVMARILGVNQKTAWKLGHAIRELMDEREAVSARLAGVVEVDEAYVGGAPKFKKGVKNKRGRGTGKPMVLVAADRTGQAKATLIPNAKGATLGP
IMKEWIDPSSALMTDSNTAYRKIGRSFASHHTVNHGKRQHSRPSKGAHINTVEAVNSQVQRALIGVYHRLGRQHLQRYLDEILWQWNHRQQEVKIRNRKT
SSGTKTIATRVWKPIPVVDQMRGLLCCAVGRQLRRTPQWGLCWPIHLFEHKGTFA
Blast result :
Comments
There is one copy of ISSpo8 in Silicibacter pomeroyi DSS-3.
ISSpo8 is 65% aa similar to ISPepr2.
The genome sequence showed that the entire IS element is significantly longer than the transposase ORF.(ISFinder annotation)
ISSpo8 is 65% aa similar to ISPepr2.
The genome sequence showed that the entire IS element is significantly longer than the transposase ORF.(ISFinder annotation)
References
1] Moran,M.A., Buchan,A., Gonzalez,J.M., Heidelberg,J.F., Whitman,W.B., Kiene,R.P., Henriksen,J.R., King,G.M., Belas,R., Fuqua,C., Brinkac,L., Lewis,M., Johri,S., Weaver,B., Pai,G.,Eisen,J.A., Rahe,E., Sheldon,W.M., Ye,W., Miller,T.R., Carlton,J., Rasko,D.A., Paulsen,I.T., Ren,Q., Daugherty,S.C., Deboy,R.T., Dodson,R.J., Durkin,A.S., Madupu,R., Nelson,W.C., Sullivan,S.A., Rosovitz,M.J., Haft,D.H., Selengut,J. and Ward,N. (2004) Nature 432 (7019), 910-913