ISSm1
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF389912 | ND | Serratia marcescens | Serratia marcescens |
DNA section
IS Length : 1369 bp
Ends
IR Length : 12/13
IRL : gaatggAAGGGACTTCCCCTGATTATGCGCCTGATTAACTCTTGTGCAAT
IRR : ---tatAAGGG-CTTCCCCGGTATGTCAACGCCGGTGTTTACGGTTTCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNCTTAGC | GTACAATAAT | 0 | |
| NNNCTTAACG | ACAATAATT | 0 |
DNA sequence
GAATGGAAGGGACTTCCCCTGATTATGCGCCTGATTAACTCTTGTGCAATCATGGGGTAAACACAGCCCCGGAGGGATTCGTTATGACCATCACGACAGT
CGGTATCGATCTTGCTAAAAATGTGTTCGCTGTTCACGGTGTTGACCAGAATGGCAAAACAGTTCTGATTAAATCCAGAGTAACCCGCACCGCTCTTCCC
GGGCTGATTGCTGGTTTGCCGCCCTGTGTTATCGGAATGGAGGCCTGTTCCGGAGCGCATTACTGGGCAAGACTGTTCCGGCAGTATGGTCATGAGCCGC
GTCTGATGGCCGCTAAATTTGTATCTCCTTACCGCATGGCCGGTAAATCAGGGAAAAATGATGCAGCTGATGCTCAGGCTATCTGCGAAGCAGTCTGCCG
ACCGCATATGCGGTTTGTGCCAGTTAAGGATGAAAGCCAGCAGGCGATGCAGTGCCTGCACCGCACCCGTCAGGGATTTATCGAAGAGAAAACAGCAACG
TATAATCGCCTGCGAGGATTGATATCTGAATTCGGCGTCATTGCGCCACAAAGCACTGATGCCCTGCGACACATGGTTTCTGCACAGAAGAATTCTTTAC
CACTTCAGGTTCAGCAATGTGTCGATGATTTGCTGGAGCACGTTGCTCGCATCGAAGTCAACATAGCTGAATATGACCGAATTTTGTCCCGCATGGCCAA
AACAGATCATCGCAGTCAGCGGCTGATGGAATTGAAGGGCGTTGGCCCTACAACGGCCTGTGCGCTGGTCGCCAGTATCGGTAATGCACATGATTTTAAG
AATGGGCGCCAGCTGGCAGCCTGGCTGGGACTGACACCATCGCAGTACAGCAGCGGCGGAAAGTCGAAGCTTGGCAGGATAACGAAAGCTGGCGATTCGT
ATCTGAGAACACTTCTGGTTCAGGGTGCCCGCTCGGTACTGATCGGGGCGGAGAAAAGGACTGATTCATTCAGTCGTTGGGTCTGCTCACAGGTCGAACG
CAGAGGATACTGGCGCGCTGTTGTTGCCATCGCTGCCAAAAATGCAAGACTGTGCTGGGCATCATTGCATTACGGTGATGATTTCAGGTTGTACTCAGCC
AGCTAAAGCACGAAGCAGCAGTATAACCATCTGGCCTGCAACTCGTTGATGATAAAGGGTTAGACCCCGTGAGGTATATCTGATTATTGTACAGGATATA
CGTATCCGCTTAACGAACAAGAGCCTCACGAGCGTCTTTCATCAGGGTCCGAATCGATGACGATTCATCATGACCGTTTATAGTACCGCAGTCTCTCCCC
TTTATTTTTAACTGTAGTGCAGACAGAAACCGTAAACACCGGCGTTGACATACCGGGGAAGCCCTTATA
CGGTATCGATCTTGCTAAAAATGTGTTCGCTGTTCACGGTGTTGACCAGAATGGCAAAACAGTTCTGATTAAATCCAGAGTAACCCGCACCGCTCTTCCC
GGGCTGATTGCTGGTTTGCCGCCCTGTGTTATCGGAATGGAGGCCTGTTCCGGAGCGCATTACTGGGCAAGACTGTTCCGGCAGTATGGTCATGAGCCGC
GTCTGATGGCCGCTAAATTTGTATCTCCTTACCGCATGGCCGGTAAATCAGGGAAAAATGATGCAGCTGATGCTCAGGCTATCTGCGAAGCAGTCTGCCG
ACCGCATATGCGGTTTGTGCCAGTTAAGGATGAAAGCCAGCAGGCGATGCAGTGCCTGCACCGCACCCGTCAGGGATTTATCGAAGAGAAAACAGCAACG
TATAATCGCCTGCGAGGATTGATATCTGAATTCGGCGTCATTGCGCCACAAAGCACTGATGCCCTGCGACACATGGTTTCTGCACAGAAGAATTCTTTAC
CACTTCAGGTTCAGCAATGTGTCGATGATTTGCTGGAGCACGTTGCTCGCATCGAAGTCAACATAGCTGAATATGACCGAATTTTGTCCCGCATGGCCAA
AACAGATCATCGCAGTCAGCGGCTGATGGAATTGAAGGGCGTTGGCCCTACAACGGCCTGTGCGCTGGTCGCCAGTATCGGTAATGCACATGATTTTAAG
AATGGGCGCCAGCTGGCAGCCTGGCTGGGACTGACACCATCGCAGTACAGCAGCGGCGGAAAGTCGAAGCTTGGCAGGATAACGAAAGCTGGCGATTCGT
ATCTGAGAACACTTCTGGTTCAGGGTGCCCGCTCGGTACTGATCGGGGCGGAGAAAAGGACTGATTCATTCAGTCGTTGGGTCTGCTCACAGGTCGAACG
CAGAGGATACTGGCGCGCTGTTGTTGCCATCGCTGCCAAAAATGCAAGACTGTGCTGGGCATCATTGCATTACGGTGATGATTTCAGGTTGTACTCAGCC
AGCTAAAGCACGAAGCAGCAGTATAACCATCTGGCCTGCAACTCGTTGATGATAAAGGGTTAGACCCCGTGAGGTATATCTGATTATTGTACAGGATATA
CGTATCCGCTTAACGAACAAGAGCCTCACGAGCGTCTTTCATCAGGGTCCGAATCGATGACGATTCATCATGACCGTTTATAGTACCGCAGTCTCTCCCC
TTTATTTTTAACTGTAGTGCAGACAGAAACCGTAAACACCGGCGTTGACATACCGGGGAAGCCCTTATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 84 | 1106 | + | No |
Chemistry : DEDD
ORF sequence :
MTITTVGIDLAKNVFAVHGVDQNGKTVLIKSRVTRTALPGLIAGLPPCVIGMEACSGAHYWARLFRQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAI
CEAVCRPHMRFVPVKDESQQAMQCLHRTRQGFIEEKTATYNRLRGLISEFGVIAPQSTDALRHMVSAQKNSLPLQVQQCVDDLLEHVARIEVNIAEYDRI
LSRMAKTDHRSQRLMELKGVGPTTACALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRTLLVQGARSVLIGAEKRTDSFSRWV
CSQVERRGYWRAVVAIAAKNARLCWASLHYGDDFRLYSAS
CEAVCRPHMRFVPVKDESQQAMQCLHRTRQGFIEEKTATYNRLRGLISEFGVIAPQSTDALRHMVSAQKNSLPLQVQQCVDDLLEHVARIEVNIAEYDRI
LSRMAKTDHRSQRLMELKGVGPTTACALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRTLLVQGARSVLIGAEKRTDSFSRWV
CSQVERRGYWRAVVAIAAKNARLCWASLHYGDDFRLYSAS
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 6 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 5 nt on the left and 4 on the right.
ISSm1 is inserted within a 38 bp sequence corresponding to TIRtnp of Tn6901, one side of the 7th base in from the end of the transposon.
The transposase protein is 94% identical to that of IS1618 from Yersina pestis.
By analogy with IS4321, ISSm1 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
ISSm1 is inserted within a 38 bp sequence corresponding to TIRtnp of Tn6901, one side of the 7th base in from the end of the transposon.
The transposase protein is 94% identical to that of IS1618 from Yersina pestis.
By analogy with IS4321, ISSm1 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Horng et al. (2002) Mol. Microbiol. 45, 1655-1671
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384