ISShvi3

  • Family IS91
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_014012 ND Shewanella violacea
Shewanella violacea DSS12
DNA section
IS Length : 2319 bp

Ends


oriIS : GAAGCCGCTATGCGGCAGAAAGATAAAACTATCTCTATCCTCTCATCCTAAATACTTACTAGTGCAGGCCTTGTTAGGCCATTAATACTAGGAGTATTGT II struct. : No
terIS : CTAGTTAAAAGTGTCACTTAAGATAATCCACCAAAACCAGCGTGAACTCTCGCGCCACTATTTACTATATAAAGGTATCGCTTCTAGTAAGCTGGGGCTT II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TTGGCTTGTT GAACGTTCAACACTTGGG
TTGGCTTGTT GAACGTTCAACACTTGGG

DNA sequence

GAAGCCGCTATGCGGCAGAAAGATAAAACTATCTCTATCCTCTCATCCTAAATACTTACTAGTGCAGGCCTTGTTAGGCCATTAATACTAGGAGTATTGT
CATGAACACATCCGAACAACAACGCTTTGATTTTCTTTATGAACAACATCTTACCAACTTAACCCTGCAAGGCAAGCGACCCGCAACCATCGACGCATAC
AGTCGTGCTGTAAGGCGCATTGCAGCATTCTTTGACTGCTGTCCCGATAATCTCACAACCGATGATTTGAAGCGCTATTTTGCTTCTTTAATCGATTCAC
ACTCTTGGAGCACCGTGAAGTTAGACCGTAATGGCTTACAGTTTTTTTATCGTTACGTGCTTAACCACAGCTGGGAGTGGCTCAACATCGTCAAGCCGCC
CCAAGTGCAGCGCTTGCCCGATATTCTTACGCCTTGTGAGGTTGCCATCGTCATTAGCCTGACTCGACAACTGCGATATCAGGTCTGCTTTTTGACCTTG
TACAGCATGGGATTACGACTCGGTGAAGCGATTTCATTGCGTGTTGGCGATATCGATTCACAAATAATGCAAGTGCATATTCGTAATGCTAAAGGCGGCA
AGGATAGATTGGTTCCTCTGCCAAAACGTACCTTGCAGGCTTTACGTTATTACTGGAAAACCCATCACCATCCTCGTTATCTCTTTCCTGGTAAAGATAG
CCAGCCAGACTCATTGATAGATAAAGGCGGTATTCAGAAAGCGCTAAAGCGGGTTATCGGAGAGTGCAATATTCATAAATCCATCAGCCCTCATAATTTA
CGACACAGCTATGCGACCCATCTGCTCGAACAAGGACTAGACTTGCGTTCGGTACAGAGTTTGCTTGGCCATAACAGCCTTAACACCACTGCACGCTATA
CACGGCTCACCCAAATTACCCGCAAAAATACCGTTGAAGCGATTAACCAACTTGCAGACGGCTTGGCACTGTCATGGGAGTGTGGCGCATGACGTTCATC
GACATTTTACGCGACCACCTAGATGCGTTTAATCAGGCTTACGAGCAGCAAATTACCTCTTCGATGCAACAAGCGATACACGCCATGCTCAGTTGCCGCA
GCAATACTGAGCGAACGAGTCAGTGGGCTTGCCAGAGTTGCACTCATATCGCTGACTTTCCACTTTCATGCGGCCATCGCAGTTGCCCACAGTGCCAGCA
CAACACTACGATAGATTGGCTCGCTAAACAGCAAGCTAAACTACTCCCTGTTGAATACTATATGGTCACTTTTACTTTGCCTTTTGAGCTTCGAGTCATT
GCAAAACATCAACCAGAGTCGATGTATCAAGCTATGTTCTGGGGTGCCGCCAGCGTGCTAAAGGAGTTCGCCAAAAACTCAAAGCAACTCGGAGGTGAGA
TTGGCTTTACTGGCGTATTGCATACCCATAACAGGCGGCGTGATTTTCATCCCCACATCCACTTCATCATCCCTGCAGGCAGCTTTAACAAAGACAAAAA
ACAGTGGCGCAAGAGCAAAGGCAAGTACCTGTTCAACGCCTTCAACCTTGCCACTGTATGGCGTGCACGTTTGCTAGAGCTACTGACAAATAAACTCGGG
ATCAAGTTACCAGAACACCTGCCCAACAAATGGGTGGTCGACTGCCAGCACGTTGGTAAAGGCTTGCCAGCACTCAAGTACCTGTCCCGATATCTCTATC
GCGGTGTATTGCCAGATAAATGCATAACCAGTGTCGTCAATGAACAAGTCAGCTTCGAGTATCAAAATAGCCAAACCAAAACAACTGAAATACGTACCTT
ACCAGTAACTGAATTTCTATGGTTAGTGCTTCAGCATGTTTTACCCAAAGGGTTACGGAGAGTGAGAGACTATGGACTGTTAAGAGGGAGTGCTGCCAAT
CTGCGGCAACGAATACGCTTGATGCTGGCAGTTGCAGGAGTGGTATTTGCCCCGCTCGAAAGTAGCACTAAAACTCAAGCCATACGACCTTGCCCCTGCT
GCAAACAGCCAATGAAATTTATGGGGGTTCATGGGAATAAGCATGCACGACCGACAAGTTTTATCATGCAGATAACAAGGGAGCCTAACGCCACTGTTTA
AGCAGAAGGCAGATAAAGTTAAGGCTTAAGGTACAGAGCAAGTCACAAATGAGGAGGTGTTAAGGTATTAATATTATTGAGCAATTCAATTAGCTAGCAA
TGCTAAGGGATCGCTGTGACTAGTTAAAAGTGTCACTTAAGATAATCCACCAAAACCAGCGTGAACTCTCGCGCCACTATTTACTATATAAAGGTATCGC
TTCTAGTAAGCTGGGGCTT
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
891 bp296 aa102992+No
ORF function : Accessory Gene
AG : IS91 integrase/resolvase

ORF sequence :

MNTSEQQRFDFLYEQHLTNLTLQGKRPATIDAYSRAVRRIAAFFDCCPDNLTTDDLKRYFASLIDSHSWSTVKLDRNGLQFFYRYVLNHSWEWLNIVKPP
QVQRLPDILTPCEVAIVISLTRQLRYQVCFLTLYSMGLRLGEAISLRVGDIDSQIMQVHIRNAKGGKDRLVPLPKRTLQALRYYWKTHHHPRYLFPGKDS
QPDSLIDKGGIQKALKRVIGECNIHKSISPHNLRHSYATHLLEQGLDLRSVQSLLGHNSLNTTARYTRLTQITRKNTVEAINQLADGLALSWECGA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1113 bp370 aa9892101+No
ORF function : Transposase
Chemistry : Y2

ORF sequence :

MTFIDILRDHLDAFNQAYEQQITSSMQQAIHAMLSCRSNTERTSQWACQSCTHIADFPLSCGHRSCPQCQHNTTIDWLAKQQAKLLPVEYYMVTFTLPFE
LRVIAKHQPESMYQAMFWGAASVLKEFAKNSKQLGGEIGFTGVLHTHNRRRDFHPHIHFIIPAGSFNKDKKQWRKSKGKYLFNAFNLATVWRARLLELLT
NKLGIKLPEHLPNKWVVDCQHVGKGLPALKYLSRYLYRGVLPDKCITSVVNEQVSFEYQNSQTKTTEIRTLPVTEFLWLVLQHVLPKGLRRVRDYGLLRG
SAANLRQRIRLMLAVAGVVFAPLESSTKTQAIRPCPCCKQPMKFMGVHGNKHARPTSFIMQITREPNATV

 

Blast result :
Comments
There are two copies of ISShvi3 encoded in Shewanella violaceae DSS12. Both elements insert 3' to AttC sites. Like other IS91 elements found in Shewanella this element encodes 2 proteins, a site-specific recombinase, phage integrase family and the IS91 family transposase. The transposase in ISShvi3 has 76% identity to the IS91 Tn encoded by ISSde12. The recombinase has 84% identity to the ISSde12 recombinase.
References
1] Romine, M. F. (2010) Direct submission.