ISfinder Mirror Site

ISSav7

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_003155	ND	Streptomyces avermitilis	Streptomyces avermitilis MA-4680

DNA section

IS Length : 2992 bp

Ends

IR Length : 17/22

IRL : TGTATTGACCACGAGCGTTGTTAACAGTGGGCGGCGGAGACGTGATCAAG
IRR : TGTATTGACCCGCAGGATTGTTGACGCGGCTGATGGGTGGTTGGCCGTCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGCCCGACTCCGACGAGA	TCTAGC	GGTTCGGTCAAGCC	6

DNA sequence

TGTATTGACCACGAGCGTTGTTAACAGTGGGCGGCGGAGACGTGATCAAGGCTGGAGGCGCGTTATGGGCAAGGGGGAGCCAGCCGGAGCCGTGTGGTCG
CCCACTGTCTCATCTGGTGGACACCGCTTCATCGCACGATCTCTCCTCGGAAGGTTCAACCTGCACGAGGGCGCCTGAGCGCGATCAGGCATGCGGCGCG
CCACCGTCCGCCGCCAGGCGCGTGAGCCACTCACGCAGCAGGCGCTGCTCGGCGTCGCTCAGGACATCGGCGTCGTCGGGGAGGGCGGCGCGCAGCGCGC
GGGCCGCTGCGGCGGGACCGGACTCCGCGGTGGGGACTGTCGGCTCGGCGCGGGTGACGGCCGCGACCATGGACTCGCGCAGGACGGTCAACAGCGCCGG
ATTGCGGCGGTCCGCAGGGGTGGAGTGCCAGGTGGTGACCGCCCCCTGGCCGGTGGCCTGGATGATCTGGGCGGCCAGCTCCTCGTCGACCCGCAGCCAT
CCCCCGGCCGCCAGCCGACGCACCCGCCCATGGAGGATCTCCAGGCCCGCGCGGTGTGCTGCGTCCGATCCCCGGCCGGTGGCCCTGTTCATCACCGCGA
ACAACTCGGGGCGACAGACCCCGAACTCGACCACCATGTCCCAGCCGCGGCGCAGCTCCTCCACCGGGTCCTGCGGAGCGGGGTCGAGCTGCGCGCGCTT
TCTCTCCAGGAACTGCGCGTAGCCGTGCTCGGCGACGGCCTCAAGGAGCCCCTCCTTGTCGCCGAAGAGGCGGTAGATCGCCGGCGGCTGCATTCCGGCC
GCGGCGGCGACCGCGCGGGTGCTCATCGCGTCAGGGCCACCGTTCTCCAGCAGCTCGACGGCCGCCTCGACGATGCGGCGCCGAGGGGTGTCGGTGGAGT
CGCGCGTAGCCATGAACCGATGGTACCGAAGATTTGGTTCCACCGGAATTTCCAGTGTTACCGTTGAAGCGATTCCAGCGGAAATAGTGTCGGGAGAACC
CAATGATCATCGTGACCGGAGCCACCGGAAAGCTCGGCCGCCGCATCGTCGAGCGCCTCCTGGAGCGCGTCCCCGCCGACCGCGTCGGCGTCAGCGTCCG
CGACCCCCGCAAGGCCCAGGACCTCGCCGACCGCGGCGTCCGCGTCCGGCAGGGGAGCTTCGACGACCCCGCCTCGCTCGTGCACGCCTTCGAGGGCGCC
GAGCAACTGCTCCTCGTCTCCCTCGACCGCACGGGCCAGGAGTGCGTCAGCGGCCACCGCGCCGCCATCGACGCCGCCGTGAAGGCCGGCGTCGGCCGCA
TCCTCTACACCAGCCAGATGGGCGCCGCCCACGACTCCCGGTTCCAGGCATGCCGCGACCACGCCCAGACCGAGGACCTGCTGCGCGCCACCGGCCTGCC
CTGGACCGCGCTGCGCAACGGCTTCTACGCCTCCAGCGCCCTGCAGTTCCTGGAGTCCGCCCGCCACACCGGCGACATCGCCCTTCCCGCCGACGGCCCC
GTCGCCTGGACCGGCCACGACGACCTCGCCGAGGCCACCGCGGCGATCCTCGCCGAGGAGCACCGCTTCGAGGGGCCCACCCCGCCGCTCACCGGCCCGG
CGGCGCTGGACTTCGACACCGTCGCCGAGATCGCGTCCCAGACCACCGGACGGCCCTTCACCCGCACCGTCGTCCCCGACGACGCCTTCCGCGAGCAGGT
CCTGGCGCACGGCGCCCCGGCCCCGATCGGCAACCTGATGCTGAGCATCTTCGCCGCGGCGCGGAATGGCGAGTTCACCGCTGTCGACTCGACGCTGGCC
GAGCTGATCGGGCGAGAGCCCGCCACCTTCCGCACCCAGCTGGAGCATGCCTGGGCCGAATAGATCCAGTGGGGCGCGCAGACGGCCCCGGCCTGACTCG
CCGCCCACAAAAGGCTCGCCCTCTATCGCCCGAGGACGTCGATCACCAGATCGGCGGCCTCGGGCACAAACTCAGACTCCGCATCAGATTCCGCACCATC
CCAGAGGTTTCCCGTGCCCCACCCCCATGCCCGGCTGACCGTCCACGGCAGACGGCTCCTCGTCGACCGGGTCCGCGCTGGCAGGCCCATCGCCCACGTC
GCCGAAGAAATGGGCATCTCCCGCACCACAGCCCACAAATGGGTCCGGCGCTGGCGAGCCGAAGGCGATCCGGGGTTGTATGACCGTTCCAGTCGACCCC
TGACGACACCGCACCGAACCCCTGCCGATGTCGAGAATCGCATCTGCGAGCTACGACGCGACCGCAAGCTCGGTCCTGCACGGATCGGCCCGGTCCTGGG
CATACCCAGCTCGACGGTCCACCGTGTCCTGACCCACTACGGCCTGAACCGGTTGCGCTGGATGGACCGGCCCACCGGTCAGATCATCCGACGCTACGAA
CGATCACATCCCGGCGAACTGGTCGACGTCGACATCAAGAAGCTCGGCAACATTCCAGACGGCGGCGGACATCGGATCATGCCCCGCCAGCAGGCCGCGG
GCAATCGGCAGGCCACAACTGACGTGCGTAGGGGCGGCAGTCCCGTGATCGGCTACAGCTTCGTTCACACAGCCGTCGACGATCACTCCCGGCTCGCGTA
CAGCGAGGTTCTGACCGACGAACGCAAAGAGACCGCGGCCTCCTTCTGGCAGCGCGCGAATACCTTCTTCGCCGAGCACGGCATCAGCGTCGAGCGCGTC
CTCACCGACAACGGCTCCTGCTACAGATCCCGCCTGTTCGGCCAGACTCTCAGCGCCACCGGCATCGTCCATAAGCGCACGCGCCCCTATCGCCCGCAGA
CGAACGGCAAGGTGGAGCGCCTAAATCGCACCCTGCTCGACGAATGGGCATACGTGCGCCCGTACTCCAGCAACGCCGAACGCACCGAAGCCCTGACGGA
CTTCCTCCACACCTACAACTACCACCGGTGCCACACCGCACTTGACGGCCAACCACCCATCAGCCGCGTCAACAATCCTGCGGGTCAATACA

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
759 bp	252 aa	943	185	-	No

ORF function : Passenger Gene
Annotation : Transcriptional regulator, TetR familyDescription : Transcriptional Regulator factor

ORF sequence :

MEPNLRYHRFMATRDSTDTPRRRIVEAAVELLENGGPDAMSTRAVAAAAGMQPPAIYRLFGDKEGLLEAVAEHGYAQFLERKRAQLDPAPQDPVEELRRG
WDMVVEFGVCRPELFAVMNRATGRGSDAAHRAGLEILHGRVRRLAAGGWLRVDEELAAQIIQATGQGAVTTWHSTPADRRNPALLTVLRESMVAAVTRAE
PTVPTAESGPAAAARALRAALPDDADVLSDAEQRLLREWLTRLAADGGAPHA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
861 bp	286 aa	1003	1863	+	No

ORF function : Passenger Gene
Annotation : Dihydrodipicolinate reductaseDescription :

ORF sequence :

MIIVTGATGKLGRRIVERLLERVPADRVGVSVRDPRKAQDLADRGVRVRQGSFDDPASLVHAFEGAEQLLLVSLDRTGQECVSGHRAAIDAAVKAGVGRI
LYTSQMGAAHDSRFQACRDHAQTEDLLRATGLPWTALRNGFYASSALQFLESARHTGDIALPADGPVAWTGHDDLAEATAAILAEEHRFEGPTPPLTGPA
ALDFDTVAEIASQTTGRPFTRTVVPDDAFREQVLAHGAPAPIGNLMLSIFAAARNGEFTAVDSTLAELIGREPATFRTQLEHAWAE

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
984 bp	327 aa	2014	2997	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPHPHARLTVHGRRLLVDRVRAGRPIAHVAEEMGISRTTAHKWVRRWRAEGDPGLYDRSSRPLTTPHRTPADVENRICELRRDRKLGPARIGPVLGIPSS
TVHRVLTHYGLNRLRWMDRPTGQIIRRYERSHPGELVDVDIKKLGNIPDGGGHRIMPRQQAAGNRQATTDVRRGGSPVIGYSFVHTAVDDHSRLAYSEVL
TDERKETAASFWQRANTFFAEHGISVERVLTDNGSCYRSRLFGQTLSATGIVHKRTRPYRPQTNGKVERLNRTLLDEWAYVRPYSSNAERTEALTDFLHT
YNYHRCHTALDGQPPISRVNNPAGQYI

Blast result :

Comments

ISSav7 transposase (third ORF) is 69% aa similar to ISMysp4. The STOP codon is in the insertion site. The first ORF is annotated as a TetR family transcriptional regulator and the second as a Dihydrodipicolinate reductase.
This IS is inserted into a monooxygenase gene.

References

1] ISfinder annotation (2008)
2] Ikeda,H., Ishikawa,J., Hanamoto,A., Shinose,M., Kikuchi,H., Shiba,T., Sakaki,Y., Hattori,M. and Omura,S. (2003) Nat. Biotechnol. 21 (5), 526-531
3]Omura,S., Ikeda,H., Ishikawa,J., Hanamoto,A., Takahashi,C., Shinose,M., Takahashi,Y., Horikawa,H., Nakazawa,H., Osonoe,T., Kikuchi,H., Shiba,T., Sakaki,Y. and Hattori,M. (2001) Proc. Natl. Acad. Sci. U.S.A. 98 (21), 12215-12220