ISSa4
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF165983 | ND | Streptococcus agalactiae | Streptococcus agalactiae BSP2001 |
DNA section
IS Length : 962 bp
Ends
IR Length : 24
IRL : CCCGTGGTGCTAGTTAATCTCAAAAATACAATAAAAAGCCTAAAAGGACT
IRR : CCCGTGGTGCTAGTTAATCTCAAAGTATCTTAGATTATAAGCCAGTATCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAGACCAAAA | TTAAGATAA | TAACTGATAA | 9 |
DNA sequence
CCCGTGGTGCTAGTTAATCTCAAAAATACAATAAAAAGCCTAAAAGGACTATACTGTGAGTGCCTAAACAAACAGGAGAGTTAGTCCCAATGAGCCACTT
ACAGTATACAGCTAAATCTCATCACTTACAATGGAATTTGAAGCAATTATCAAAAATATGCCATCAGCTGTATAGGGATTATTGTCCTGAATCTTTCAAA
CATCGTCACAATATCAGTTTATCTAAGGTTTCAGATCAATCACTATTGGTTTTGCTTATTTTACAAGCTGAGCTAGGTATTAAGTCGCAACGACACTTCT
ATCGTATCTGTCACTTATTTCCCTGCGGGTGTCTCCTAGAAAGAAGTCGCTTCAATCGGCGAGCAAGACAGTTGATTTGGTTAGTTCAAATCATTAGACA
AGCCATGAATAGTCACATGTCTCATGAGACCATCGTCATCATAGACAGTTTGCCCTTACTGCTTTGTCAACCTATCCGTCATCATAGAGTTCGTATTTTT
GAAGGATTAGCAGATATCGGTTACAATGCTTCCAAACACCTATGGTTCTATGGGTTTAAAGTACATATGCTGGTTACTTTATCTGGCTATATCCTGAACT
ATGTTGTGACATCGGCTTCTGTTCATGACATCAGAGCAGTTGATGACTTACTAGAAAATTGCCGACAACCTTATATTTTAGCTGATTTAGGCTATCTTAG
TAGAGACCTTAAAGACCATTTGGTTCAAAAAGGCTATCATCTTTGGACTCCTTTACGACAAAACATGGAAGGGTCTAAACAACATAACCATTGGAAATTA
ATGACCATGAGACGAACCATTGAGACACGCTTTTCAGAACTTTGCAGTCTTTTTGATATAGAACACACTCTGACTAGAGGTTTAACAGGACTACAGTTAA
GTATTGAGCAAATGATACTGGCTTATAATCTAAGATACTTTGAGATTAACTAGCACCACGGG
ACAGTATACAGCTAAATCTCATCACTTACAATGGAATTTGAAGCAATTATCAAAAATATGCCATCAGCTGTATAGGGATTATTGTCCTGAATCTTTCAAA
CATCGTCACAATATCAGTTTATCTAAGGTTTCAGATCAATCACTATTGGTTTTGCTTATTTTACAAGCTGAGCTAGGTATTAAGTCGCAACGACACTTCT
ATCGTATCTGTCACTTATTTCCCTGCGGGTGTCTCCTAGAAAGAAGTCGCTTCAATCGGCGAGCAAGACAGTTGATTTGGTTAGTTCAAATCATTAGACA
AGCCATGAATAGTCACATGTCTCATGAGACCATCGTCATCATAGACAGTTTGCCCTTACTGCTTTGTCAACCTATCCGTCATCATAGAGTTCGTATTTTT
GAAGGATTAGCAGATATCGGTTACAATGCTTCCAAACACCTATGGTTCTATGGGTTTAAAGTACATATGCTGGTTACTTTATCTGGCTATATCCTGAACT
ATGTTGTGACATCGGCTTCTGTTCATGACATCAGAGCAGTTGATGACTTACTAGAAAATTGCCGACAACCTTATATTTTAGCTGATTTAGGCTATCTTAG
TAGAGACCTTAAAGACCATTTGGTTCAAAAAGGCTATCATCTTTGGACTCCTTTACGACAAAACATGGAAGGGTCTAAACAACATAACCATTGGAAATTA
ATGACCATGAGACGAACCATTGAGACACGCTTTTCAGAACTTTGCAGTCTTTTTGATATAGAACACACTCTGACTAGAGGTTTAACAGGACTACAGTTAA
GTATTGAGCAAATGATACTGGCTTATAATCTAAGATACTTTGAGATTAACTAGCACCACGGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 864 bp | 287 aa | 90 | 953 | + | No |
Chemistry : DDE
ORF sequence :
MSHLQYTAKSHHLQWNLKQLSKICHQLYRDYCPESFKHRHNISLSKVSDQSLLVLLILQAELGIKSQRHFYRICHLFPCGCLLERSRFNRRARQLIWLVQ
IIRQAMNSHMSHETIVIIDSLPLLLCQPIRHHRVRIFEGLADIGYNASKHLWFYGFKVHMLVTLSGYILNYVVTSASVHDIRAVDDLLENCRQPYILADL
GYLSRDLKDHLVQKGYHLWTPLRQNMEGSKQHNHWKLMTMRRTIETRFSELCSLFDIEHTLTRGLTGLQLSIEQMILAYNLRYFEIN
IIRQAMNSHMSHETIVIIDSLPLLLCQPIRHHRVRIFEGLADIGYNASKHLWFYGFKVHMLVTLSGYILNYVVTSASVHDIRAVDDLLENCRQPYILADL
GYLSRDLKDHLVQKGYHLWTPLRQNMEGSKQHNHWKLMTMRRTIETRFSELCSLFDIEHTLTRGLTGLQLSIEQMILAYNLRYFEIN
Blast result :
Comments
ISSa4 is 69% aa similar with ISLh1.
The transposase end is in the IRR.
ISSa4 could be detected only in strains isolated after 1996 which might indicate a recent acquisition of this IS by Streptococcus agalactiae.
The number of copies of this IS varies between strains : Streptococcus agalactiae BSP2001 harbored more than 15 copies of ISSa4. One had occured 87bp downstream of the ATG start codon of cylB.
The transposase end is in the IRR.
ISSa4 could be detected only in strains isolated after 1996 which might indicate a recent acquisition of this IS by Streptococcus agalactiae.
The number of copies of this IS varies between strains : Streptococcus agalactiae BSP2001 harbored more than 15 copies of ISSa4. One had occured 87bp downstream of the ATG start codon of cylB.
References
1] Spellerberg,B., Martin,S., Franken,C., Berner,R. and Luetticken,R. (2000) Gene 241 (1), 51-56