ISfinder Mirror Site

ISRpa1

Family IS1595
Group ISNwi1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007778	ND	Rhodopseudomonas palustris	Rhodopseudomonas palustris HaA2

DNA section

IS Length : 2091 bp

Ends

IR Length : 27/28

IRL : CCCAATATTATACTTTGCGTACCCAAAATTCTGTGAGATATTGGCTTCAA
IRR : CCCAATATTATACTTTGCGTACCCACAAAACCCGGTGCTACCATGAGTAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACGAACGAGAGCTAGG	TTTTTTTT	CCTAAATTGCT	8

DNA sequence

CCCAATATTATACTTTGCGTACCCAAAATTCTGTGAGATATTGGCTTCAACACGAGGTTGAAGCCATGAAGTCCTTTTTGAACGCCAAGCACCTGCAGAA
CGAAGAAGCGGCCTACGCTTGGGTTGAAGCCCGTATTTGGCCCAACGGCCCGGTTTGCCCGCACTGTGGCGGCGTTGACCGCATTTCCAAGATGCAGGGC
AAGTCCACTCGGATCGGCGCTTACAAGTGCTATCAGTGCCGCAAGCCCTTTACCGTCAAGGTTGGCACCATCTTCGAATCCAGCCACGTCCCCATGCACC
TGTGGCTGCAGGCGATCTTCCTGCTTTCGTCCTCAAAGAAGGGGATCAGCAGCAACCAGCTTCACCGCACCCTTGGCTGCACCCTCAAAACCGCGTGGTT
CATTTCTCACCGCGTCCGCGAAGCGATGCGCGATGGTGGCCTTGCCCCGATGGGCGGCGCTGGCGGAATTGTCGAGGTTGACGAAACCTATTTCGGCAAG
ACCAAAGAAAAGAAGCCGTCACCGCAGCGCAAGGGCCGTCCTTTCATTCATCGTGGCGGCGGCCCGTCCGGCAAGCGCGCTATCGTTTCGCTTGTCGAGC
GCGGCGGCAAAGTGCGCTCGTTCCATGTCGAGAACGCCGACAAGCCGACTGTCGTTGGCATCGTTACTGCCAACGTCGCGAAAGAAAGCCGCCTGCACAC
CGACGAAAGCCGCCTCTACATCGGCGCGGACCAACACTTTTCCTCTCACGAAACTATCAATCACACCGCCAAGGAATACGCTCGCGGCGATGTCACCACC
AATTCTGTTGAAGGCTTCTTTTCGATCTTCAAGCGCGGCATGAAGGGCGTCTATCAGCACTGCGGCGAAAACCATCTTCATCGCTATCTGTCGGAATACG
ATTTCCGCTATAACAACCGCGTCGCACTTGGCGTGAACGACTACGAGCGCGCCGACCGTGTGTTGGCTGGCGTGGTTGGCAAGCGTCTCACCTATCAAAC
GACTCGTTAAAGGCAAAATTCGATGGATAAGAAGCCAAAAGCTCGCGGCCCTACGAAGAAAGCAGCGAAGCCTAAAGCGAAAGATGAAAAGCAATTCGAG
CGGTTTATAGAAACCGCTCGTGAGCTTGATGTTGATGAGAGCGGGAAGAGCTTTGATGTAGCGTTTAGGAAGATTGCCACGCCGACAGTGAGGACTAAAT
CAAAATCGCCAACTGGTTCATGAAGGTGCGAACGCACTGAAATATATCTTTGCTTTGTTCCGGCGAGTAAGTCGAGATCGGGTGCATCGTGCTGTTACGC
CATGCTTGCTTCACATGGTAAAGATTTGCGTGACATTCTGACCATGCGTCGCGCTTCTTGTCTTTGGGCATCGCGCCGATTTTAGCGTGCAAGTCGCTCA
ATAGCTTACCCCACTCTCTATCGGGGTTAGGGATCTGCAAAGAGGTGCACAACTCCCCGACCGCACATTCCATCGCTCTCATGAGGTGAAATACTGAGGC
GGTGTCCTGACCAAGGGCGATGCACTTGCCCGCATTTTCAATGTCGGGAATCGAGTTGGGAAAACGCTCGGTTACTCGGTTTCCGAATTGGTCCTTATCT
TCGTAATACCGCACTAACTCTGGCCGAACGTAGTACATTTTGTGAGCAGGCAATTCGTCTTGGATGCGGTTTCTCAGATCGGTAACGAGTCCAAGAATTT
CACTAAGTTGATTGCTAGACATCGGAGGGGTAAACGAGCCTATGGCCCGCCAGACGCGTTGTACTTGAGCCTCAACCGACGGCATAGACATCGGCAGCTT
AAGGACGATGGTTTTGCTATCGTCCTCGTCCCTCGAAATTATTTTTTCCCAGATAGGGGCGAGGCGGGCTTCCAAAGTTGCCCCAGAAGATATGTCTAGC
GCCTTTACTACGTCAGGGTTCGATATTCCGCCTCCCTTTTCCATCTCGGCAATGAGTTGAGCGATAAAGAAGAATTCAGATACGTATGCCCGCAACATAT
CAAACAAGCTCCATAGCTTGCCGGGCTGGACGCCCCCGGCGCTACTCATGGTAGCACCGGGTTTTGTGGGTACGCAAAGTATAATATTGGG

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
945 bp	314 aa	66	1010	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKSFLNAKHLQNEEAAYAWVEARIWPNGPVCPHCGGVDRISKMQGKSTRIGAYKCYQCRKPFTVKVGTIFESSHVPMHLWLQAIFLLSSSKKGISSNQLH
RTLGCTLKTAWFISHRVREAMRDGGLAPMGGAGGIVEVDETYFGKTKEKKPSPQRKGRPFIHRGGGPSGKRAIVSLVERGGKVRSFHVENADKPTVVGIV
TANVAKESRLHTDESRLYIGADQHFSSHETINHTAKEYARGDVTTNSVEGFFSIFKRGMKGVYQHCGENHLHRYLSEYDFRYNNRVALGVNDYERADRVL
AGVVGKRLTYQTTR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
855 bp	284 aa	2049	1195	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSSAGGVQPGKLWSLFDMLRAYVSEFFFIAQLIAEMEKGGGISNPDVVKALDISSGATLEARLAPIWEKIISRDEDDSKTIVLKLPMSMPSVEAQVQRVW
RAIGSFTPPMSSNQLSEILGLVTDLRNRIQDELPAHKMYYVRPELVRYYEDKDQFGNRVTERFPNSIPDIENAGKCIALGQDTASVFHLMRAMECAVGEL
CTSLQIPNPDREWGKLLSDLHAKIGAMPKDKKRDAWSECHANLYHVKQAWRNSTMHPISTYSPEQSKDIFQCVRTFMNQLAILI

Blast result :

Comments

ISRpa1 is 74% aa similar to ISNwi1. The second ORF is a passenger gene on the complementary strand.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Pelletier,D.A., Kyrpides,N., Anderson,I., Oda,Y., Harwood,C.S. and Richardson,P. US DOE Joint Genome Institute (2006) Direct submission GenBank.