ISfinder Mirror Site

ISRop1

Family IS1380
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012522	ND	Rhodococcus opacus	Rhodococcus opacus B4

DNA section

IS Length : 4160 bp

Ends

IR Length : 28/34

IRL : CCTGGATCCACCGATGTGGTGTCGGGGTAGGTGAGTCGAAACGGCGAGAT
IRR : CCCGGATCCACCGATGTGGTGCGGGCCGGGTGATGGGTTCGGTTGCGGCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTGAGTACTT	ATTAA	CCGCCCGCGG	5

DNA sequence

CCTGGATCCACCGATGTGGTGTCGGGGTAGGTGAGTCGAAACGGCGAGATGCCCTTGCGTGCTGAAAGAATTGGACTTCTCACAGAACCAGTTCGATCGA
CACGAAGGGCATCTCTAGGGTGGTGCGACACGAGGTCGCATGCGTCAAGTGGCGCGTTGATTGGAGGTCGGCGGTGTTGTCGATGACGTAGCCCGGGTTT
GTGTTCGATGGCCCGTCCCCCCACCTTGACGTGCGTCGCTGGTAACGGCGGGGTGCGAAGCTCAGGGGTAGAAGCCGAAGCTGTCCTGGATCCATCCCGG
GGCGGCAGGTGAGGGGAAGGCCGGACGGGTGAACGTGAGTGAACCGCCGAAACGTCTCGTCAAGATGAGCCTCGGATAGATGGAAGCTTGGGCCGGGGTA
TAGGGCTGGCCGTTGAGAAGCGGCAGACGGCGACCGGGGGCATCTGACCGGTCGTATGAACACCGTCGCCCCGGGATCGAGGCGGCACCCACCCCGGCCG
GGTCTTGCGCATGTGGAACGTGGAAACCCCGCTCTGGTCCGGACTGGTGGTTCGGTGGGCCGACCGTGAGGAAGGCTGAACGCCTGGGCGGGAACAGGAT
GGCCCAAGAAGCTAATGCCGGTGGCCGAAAGGCGACAGGAAACCGACCCGATTTGATGTCGTCTCCTCCGACGGCGGGGTTCGGATAACTGGCCGGATAC
GAGTGAACACTCGAACCCGTGGAGGGTGCTGACGTGGGCCAGGTGAGCCTTTGAAAGGGACGACGATGACAGCGCCAGAACTTGCGGACAAGTTGGACAC
CACTTCGGTGAACGGACCACAAGACGTCATGACGAACTGGGATGCGATCTCGTGGCGCTCGGTGGAGCAGGACGTACGGCGGCTGCGGCGGAGGATTTTC
AAGGCGTCGCAAGAAGGGGATCTCAAACGGGTCCGCAACTTGCAGAAACTGATGCTGCGAAGCCAGTCGAACACGCTCCACAGTGTGCGGCGGGTGACGC
AGCGCAACGCTGGACGCAGGACCGCGGGCATCGATGGAGAAGTCGTTTTGACTTCTTCTGCCAGGGCTGAGTTGGCGGTGGATCTGCACCGGCATACTGC
GCTGTGGCAGGCCCGACCGGTCAAGCGTGTCTACATTCCGAAGGCGAATGGAAAGCAGCGTCCACTGGGGATTCCCGTGCTTCGTGACCGGGTGCAACAA
GCTCGTGTCGCGAATGCGTTGGAGCCGGAGTGGGAAGCGCGGTTCGAACCACGCTCGTATGGGTTCCGGCCCGGGCGTGGCTGTCACGACGCGATCGAAG
CGATCTACTGGACGCTCAAAGGACGCAGATCCAAGCGCCAGTGGGTGCTCGACGCGGACCTGTCGGCAGCGTTCGACCGCATCGATCATGAGCGGCTGCT
GGCACATCTGGGAACCTTCCCCGCACGGGGGCTTGTCGCCGGATGGCTGGCTGCAGGAGTGATCGAGCAGGGCCGGTTCACCTCCACCGACGAAGGAACC
CCTCAAGGTGGGGTGATCAGCCCGATGCTGCTCAACATTGCGCTGCACGGGATGGAGGAAGCCGCTGGAGTTCGCTACACACGTAGCGACCCCCATGGGG
CGCACGTGGCAAGAGGAGCTCCGGTGCTGGTGAGATACGCCGACGACTTCGTCATCATGTGTCACACCAAGGGCCAAGCCGAACAGGCCCGGCACCGGTT
GGGTGAGTGGTTGACGCCACGGGGCCTCGAATTCAACGAAGACAAGACACGAATTGTCCACGTCGACGCAGGATTCGACTTCCTGGGGTTCAACGTCCGT
CGATACGACGGCAAACTGCTCATCAAACCCAGCGCAGCGGCAGTGAAGCGGATCCGGCATCGGCTCGCCGACGAGGTGCGTTCCCTACGGGGAACCAACT
CCGAAGCGGTGATCCGCCAACTGACCCCGATCATTCGGGGTTGGGCTGGCTACTACCGGAGCGTGGTCTCCAAAGAGGTGTTCTCCGCCGTGGATCACTA
TCTGTGGCAACACCTCTACGGGTGGGCGCTGCGCGCCCACCCGAGAAAGTCGAGGAACTGGGTCGTGGATCGATACTTCGGCACGTTCAACCCATCCAGA
ACAGACCGATGGGTGTTCGGCGACCGCGACAGCGGGAAGTACCTACGCCAGTTCGCCTGGACGAAGATCGTCCGCCACAACATGGTCATGGGCACCGCGT
CACCTGACGATCCTGCCCTGGACCAGTATTGGGCACGTCGGCGACGAAAGGCATACGCGCAACTCGGTGGTCTCACAGCCTCGCTGCTGCTCCAGCAGCG
CGGGCTATGCCCCGTGTGCGGGACATTCCTTCTGCACGCCGACCACGCTCCACAAAGCCCACAAGAGTGGGAACAGTGGATCAAGGCCATGACGAGAGCG
CTACGCGCTACTGCCCTCGTCTTGGGTAACGCCCATGGCGGCGATCGGGCCACACGCCTCACGCATGCCCACTGCCGACAACTCGAGCGACAGGGCAGTG
GGTCCAGCGGAATCAGGGGACAGACGCCCGTGGGGCTTGCTTGAGCCGGATGCGGTGAAAGTCGCACGTCCGGTTCTGAGGGGGCCTCGGCGCCGCAAGG
CGTCGGGGCTACCCGACCAACTATCCCACACCCACCCGGTGACGGCCTCGGCGTTCGACGACCGCAATCTCCTCGCCGAGGCCGGACTGATCCCGGTCCT
GGGGTTGGCTCAGGACTGCGGCCTCGATGACCTGGCAGACGAGCATCTGACGCTGCCCACCGACAAGGGCGCACACGCGGGCCGGAAGGTGACCGCACTG
GTAGCGGGCATGGTCGCCGGCGCCGACAGCATCGACGACATGGCGCTGCTGCGGCACGGCGCCATGCCCACGGTATTCGACCACCCGTACGCCCCGTCGA
CGCTGGGCTCCTTCCTGCGGACGTTCTCCTTCGGGCACGTCCGCCAACTCGACGCCGTCGCCGCCCGATTCCTGCAAGGCCTGGCCGAACGAACACCGCT
GGTCGGTGGGCCCGAAGTCGGTGGGCGGATCCTCGTCGACCTCGACGACACCATCGTCCCGGTGCACGGGCACGCCAAGCAGGGTTCGGGCTACGGATAT
ACGGGGGTCCGGGGGTTGAACGCGCTGATCGCCACCGCCACCACCGACGGGCGGGCACCGGTGATCGTCGGCCAACGCCTGCGCCGCGGCGGCTGCGGAT
CCCCACGCGGGGCATCCCGGATGGTCAGCGACGCCCTCGCCACACTCGGCCGCCTGCGCCCGCAGCTGTCACCGGGGTCTGTGCTGGTGCGGGCCGACTC
GGCCTTCTACGGGCACCCGACCGTGTCCGCGGCACTGCGCTCCGGCGCCGACGTCTCGGTCACGGTGCGGATGACCGCGACGGTGAAGGCGGCGATCGCC
GCCATCGACGAGACGGCGTGGACACCGATCGAATACACCGACGCCGTCTACGACGAACAGGCCGGCCGGTGGATCTCCCGCGCCGAGGTCGCCGAGATCG
ACTTCACCGCGTTCACCCGCCTCGCCTCCGAGCGGACACCGGGCCGGTTGGTGGTGCGCCGCATCCCCGACCTGCGGGAACACACCGATGGTCAGGGCAC
GTTGTTCGACGCCTGGCGGTTCCACGCGTTCTTCACCACCACCGACCCCGCCGTCCTGGACACCGTGGCCGCGGACAAGATCCACCGCGGGCACGCGATC
ATCGAGCAGGTCCACGCCGACCTGAAGAATTCCGCGCTCGCGCACCTGCCGTCGGGGCGGTTCGCCGCCAATAGTGCCTGGCTGGTGCTTGCGGTGATGG
CGTTCAATCTCACCCGCGCCGCCGCCACCCTCACCGGGGCCCGCCTGGTGCGGGCGACGACCGCCACCATCCGCCGGACCCTGATCTCGGTCCCGGCGCG
GATCGCGTCCTCGGCCCGCCGCCTCACCCTGCACCTGCCGCGGCGCTGGCCCTGGGAATCCGAATGGAACCAACTCTTCACCCACACCCGGCATCGAAGA
CCCCAGGTCACCTAATCCGCCCGGCCACCCCCGGCCCCGACCCGAAAGACCCAGTGGAACAACCCGACACATCGGTCGGGCGATCACCCACGCCCCGACA
GCCCATCAACGGCCGCAACCGAACCCATCACCCGGCCCGCACCACATCGGTGGATCCGGG

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1779 bp	592 aa	766	2544	+	No

ORF function : Passenger Gene
Annotation : Reverse transcriptaseDescription :

ORF sequence :

MTAPELADKLDTTSVNGPQDVMTNWDAISWRSVEQDVRRLRRRIFKASQEGDLKRVRNLQKLMLRSQSNTLHSVRRVTQRNAGRRTAGIDGEVVLTSSAR
AELAVDLHRHTALWQARPVKRVYIPKANGKQRPLGIPVLRDRVQQARVANALEPEWEARFEPRSYGFRPGRGCHDAIEAIYWTLKGRRSKRQWVLDADLS
AAFDRIDHERLLAHLGTFPARGLVAGWLAAGVIEQGRFTSTDEGTPQGGVISPMLLNIALHGMEEAAGVRYTRSDPHGAHVARGAPVLVRYADDFVIMCH
TKGQAEQARHRLGEWLTPRGLEFNEDKTRIVHVDAGFDFLGFNVRRYDGKLLIKPSAAAVKRIRHRLADEVRSLRGTNSEAVIRQLTPIIRGWAGYYRSV
VSKEVFSAVDHYLWQHLYGWALRAHPRKSRNWVVDRYFGTFNPSRTDRWVFGDRDSGKYLRQFAWTKIVRHNMVMGTASPDDPALDQYWARRRRKAYAQL
GGLTASLLLQQRGLCPVCGTFLLHADHAPQSPQEWEQWIKAMTRALRATALVLGNAHGGDRATRLTHAHCRQLERQGSGSSGIRGQTPVGLA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1461 bp	486 aa	2555	4015	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKVARPVLRGPRRRKASGLPDQLSHTHPVTASAFDDRNLLAEAGLIPVLGLAQDCGLDDLADEHLTLPTDKGAHAGRKVTALVAGMVAGADSIDDMALLR
HGAMPTVFDHPYAPSTLGSFLRTFSFGHVRQLDAVAARFLQGLAERTPLVGGPEVGGRILVDLDDTIVPVHGHAKQGSGYGYTGVRGLNALIATATTDGR
APVIVGQRLRRGGCGSPRGASRMVSDALATLGRLRPQLSPGSVLVRADSAFYGHPTVSAALRSGADVSVTVRMTATVKAAIAAIDETAWTPIEYTDAVYD
EQAGRWISRAEVAEIDFTAFTRLASERTPGRLVVRRIPDLREHTDGQGTLFDAWRFHAFFTTTDPAVLDTVAADKIHRGHAIIEQVHADLKNSALAHLPS
GRFAANSAWLVLAVMAFNLTRAAATLTGARLVRATTATIRRTLISVPARIASSARRLTLHLPRRWPWESEWNQLFTHTRHRRPQVT

Blast result :

Comments

The first ORF is a passenger gene annotated as reverse transcriptase. The second is the transposase and is 82% aa similar to ISRjo1.

References

1] Isfinder annotation (2009)
2] Sekine,M., Hosoyama,A., Kosugi,H., Yamada,R., Matsuo,Y., Tsukatani,N., Fujisawa,T., Takarada,H., Omata,S., Kishi,E., Shimizu,A., Koga,C., Saito,S., Nakazawa,H., Sekigawa,T., Nishiko,R., Takeyama,M., Tanikawa,S., Tago,S., Fujita,N., Kato,J., Na,K., Ohtake,H., Nagayasu,K. and Harayama,S. (2009) Direct submission GenBank.