ISfinder Mirror Site

ISRfsp2

Family IS110
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009523	ND	Roseiflexus sp.	Roseiflexus sp. RS1

DNA section

IS Length : 1160 bp

Ends

Left end : GCGCCTAGTATAATCTAACGGGACACGGTATACCGTTCTGCGCCAGGTTCACGAGATCGCCGCGGAGTGTTGGGTAGCCGTGTCCTTCAACGCGACCAAG
Right end : AAGCTGCTGACTATTCTCGGCGCAATATTGCGTCAGCAAAAGCCCTGGGATCCGGCTGTGCATACGAGCGCCCCTTGACAAGCAACACAGTTACTCTGCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGCGAACGCT		GTGACATACC	0
TGCGTCCACT		GTGACATGAC	0
GGAGTTCTCT		ACTCTGCGGT	0
CCGCGCCTTC		TGAGCCGGTC	0
TGCGTTTACT		GTGAGATGAT	0
CGCGCCCTCT		GTGAAAAATA	0
CCGCGCCTTC		TGAGCCGGTC	0
CGCGCCCGCT		CCCGCGCCCC	0
CCATATCTCC		TCTCCGCGTG	0
CCCGCTCTCC		TGAGCCGGTC	0
CTGGCAGTCC		GCGTACTTTC	0
CCCCATCTCC		TGAGCCGGTC	0
CCGCGCCTCC		TGAGCCGGTC	0
CCCTATCTCC		CCTCCGCGTG	0
CCGCGCCTTT		TGAGCCGGTC	0
CCGCGCCTCC		TGAGCCGGTT	0
TGCGTCCGCT		GTGAAATTGT	0
CCGCGTCTCC		TGAGACCATC	0
GAGCGCCTCC		CGTGCTCGCG	0
GGAAGACTCT		TCTCGGCGGT	0
CAGTTACTCA		GTGCGCCACT	0
CGCGCTCTCC		TGAGCCGGTC	0
CTGCCCTCCG		CGTGGTTCCG	0
CCCCATCTCC		GTGCATTACT	0
CCGCGCCTTC		TGAGCCGGTT	0
TGCGAACGCT		GTGAAATACC	0
GGCGTTCTCT		TCTAGTATAA	0
CCGCGCCTCT		TGAGCCGGTC	0

DNA sequence

GCGCCTAGTATAATCTAACGGGACACGGTATACCGTTCTGCGCCAGGTTCACGAGATCGCCGCGGAGTGTTGGGTAGCCGTGTCCTTCAACGCGACCAAG
CCGAACAGTTATCTGGGCCCTCAAACCCGCATATGCTAGCAAGGCTGGCGCCGTTTTCCTCAGGAGGTTCTGTGTATGGCTTCCCGTGAGTCGCTCTTTA
TCGGCATTGATGTCTCCAAACAGACGCTGGATGTGGCGTTTGGCGCCGACCCGCACGCGCCACGCGAGACGATACCGTCTACCGACGAAGGTGTCCAGCT
CCTGGTCACGCGACTCCAGCGCCTGCAGCCGACCCTGATTGTGCTGGAGGCGACCGGCGGGCTGGAGCGCATGGTGTTCGCCCAACTGCTCCAGGCTGGC
TTGCCGACGGCGCGGGTGCAGCCACGCCGCGTGCGCGCCCTGGCGCACGCGGAAGGACGCCAGGCGAAGACCGACCGCCTGGATGCCCGGTTGCTCGCCC
GCTTTGCCGAACGGGTGCGCCCGCCGCACCACCAAGCGACGGACGAGCAGCGCGCATCCTTGCGCGACCTGCTGGTCCGGCGGGAGCAGGTGATTCAGAT
GCGGACGGCTGAGATCAATCGGTTGACGGCTGCCGCGCCGAACCTCCGCCCGGGCATCCAGCAGCATATTGATTGGCTGGATCAGGAGATCCGTGCGCTT
GAGCAGGAACGCGACAACGAGGCGGAGCGCACCGACGAGGTGCGCCGGAAACGGGAGCTGCTCGAAAGCGTGCCCGGCATCGGCGCGATCACCGCACTGA
ACCTGCTGCTCCGCCTGCCCGAACTGGGGACCATCAATCGCAAGGAAGCGGCGGCCTTTGTGGGCGTTGCGCCGTATGCCAATCAGAGCGGCGCACAGCA
CAAACCCCGGCATATCTCCGGCGGCAGGAGGGATGTGCGCAGCGTGTTGTACATGGCGACCCTGGCGGCCACGCGGCGCCGTCTGGTCAGGCGCGCCTTC
GATCAGCGCCTGTGTCAAGCTGGCAAGCCGCGCAAGGTCGCCATCGTCGCTGCGATGCGCAAGCTGCTGACTATTCTCGGCGCAATATTGCGTCAGCAAA
AGCCCTGGGATCCGGCTGTGCATACGAGCGCCCCTTGACAAGCAACACAGTTACTCTGCG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
963 bp	320 aa	176	1138	+	No

ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MASRESLFIGIDVSKQTLDVAFGADPHAPRETIPSTDEGVQLLVTRLQRLQPTLIVLEATGGLERMVFAQLLQAGLPTARVQPRRVRALAHAEGRQAKTD
RLDARLLARFAERVRPPHHQATDEQRASLRDLLVRREQVIQMRTAEINRLTAAAPNLRPGIQQHIDWLDQEIRALEQERDNEAERTDEVRRKRELLESVP
GIGAITALNLLLRLPELGTINRKEAAAFVGVAPYANQSGAQHKPRHISGGRRDVRSVLYMATLAATRRRLVRRAFDQRLCQAGKPRKVAIVAAMRKLLTI
LGAILRQQKPWDPAVHTSAP

Blast result :

Comments

ISRfsp2 is 61% aa similar to ISGme8.

References

1] ISfinder annotation (2011)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Bryant,D. and Richardson,P. (2007) Direct submission GenBank.