ISfinder Mirror Site

ISPre3

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_004444	ND	Pseudomonas resinovorans	Pseudomonas resinovorans plasmid pCAR1

DNA section

IS Length : 2957 bp

Ends

IR Length : 17/24

IRL : GTAAGCGTCCGCCCTGCACCCACTGCCTGCCCTAGCCTTGAATAGCCACA
IRR : GTAAGCGTACGCTCGCTACCGTCTTGACCCTCGGGTCTTAATCCGCTTCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCCGCCTCCA	GAATAATT	GGCCTTTTGA	8
CTTTCAGCGG	GGTACATA	TCTGCTGAGG	8

DNA sequence

GTAAGCGTCCGCCCTGCACCCACTGCCTGCCCTAGCCTTGAATAGCCACAATGGTGCCCACCATTTTTTAGTGGGCACCATTGTGGATACGCTCCCTTCT
ACTCCTCGTCGCAGTCGACGCCCCAACTTTTCGGTGGAGTTCAAGCGTCGTGTCGTCGAGGCCACGCTATTGCCCGGCGCATCAGTCGCGTTGATCGCCC
GAGAGCATGAGATCAATGCCAATCTCGTCTTCAAATGGCGCCGTCACTATCGGGAGGGGCAACTGGGGTCAGTCGCCCAGCAGGCCACTTTGTTACCGGT
CAATCTGAGCAAGGCGCCGACGTCTTCAGCGGAAGAGGCCGTGCGACTGCCGCTGAGTCCGGGCGGTCTGGTCGTGGAGTGCGGTCGGGTCACCTTGCGC
ATTGAGGGCGTGCCCGACCCGCAAACCTTGCAACTGGTCTTGCAGCAGGTGTTGCGATGATTGCTCCGCCCATCGGGACTCGCATTTGGATCGCTGCCGG
GGTCACGGACATGCGGCGGGGGTTCGATGGCCTGGCGGCCCTGGTGCAAACTCAGCTTGAAGCGGATCCATTCTCTGGCCAGATCTTTGTGTTTCGAGGC
CGACGGGGTGACCGGATTAAATTGCTGTGGTGGGATGGCGATGGCTTGTGTTTGTTCTGCAAGCGGCTCGAGCAGGGGCGCTTCGTCTGGCCGCAGGCCG
CCAGCGGCAGCGTATCCTTGACGACGGCACAACTCGCGATGCTGTTGGAGGGCATCGATTGGCGCCGACCGATACGCACCGCACCGGTACGAACGGTCTG
ATTTTCAACGCAGGAGAACACCAGTAACATCGCGCCATGACCTGCGCGACTGACTGCCTCCCTGACGACATCCAGGCCTTGAAAGCCTTGGTAACCGCCC
AGCGCGGGGAAATCGAGCACCTGAAACTGATGATCGCCAAGCTGCGGCGCACGCAGTTTGGTCGGCGTTCTGAGCAACTCGATGGCATGCTCGACCAACT
GCAACTGACGCTCGAGGAGTTGCAGGTCAGCGAGGCCGCACTGGCCTCGCCACCACCAGCCGAATCGCATCCACGTCCCCCTGTACGACGCAAGCCATTG
CCCGCGCACTTGCCGCGTGAAATCCACGTCCATCGACCCGATACGCAGTGTCCTGGCTGTGGCGGCGAACTTCGCCATTTGGGTGATGACGTGGCTGAAG
TTCTGGAGTACGTGCCGGCACGGTTCAAGGTGGTTCGCCATGTGCGGCCCAAGTTGGCTTGCCGCTGCTGTGACGGGATTGTGCAGGTTCCTGCCCCTAG
CCGCCCGATTGCCCGGGGGCTTGCCGGGCCAGGATTGTTGGCCCATGTGCTGGTGTCCAAATACGTGGATCATCTGCCGCTGTATCGGCAGTCCGAAATC
TATGCGCGCGAGGGCGTGTCCCTGGAGCGCTCGACCATGGCCGACTGGGTCGGCGAAGCGAGCCGGCTCTTGCAGCCATTGGTCGGCCGGTTGCGGCAGC
ACGTCATGACCAGCAACAAAGTCCACGCCGACGATACGCCGATCGCCGTGCTCGCCCCCGGCCAGGGTAAAACCAAGACCGGACGCTTGTGGACGTATGT
GCGTGACGAGCGCCCCACAGGTAGTAGCACACCTGCGGCGGTCTGGTTTGCCTACTCGCCGGATCGCAAAGGCGAGCACCCCCGAGCCCATCTCAAAGGC
TTCGCCGGGACATTACAAGCCGATGGTTATGCCGGTTTCGCTCAACTCTATGCCGCGGGCACGATTCACGAGGCGGCTTGTTGGGCGCATGCCCGGCGCA
AGTTTTTCGACCTGCACAAGGCATTGGCCTCACCGATCGCCGCTGAAGCCTTGCAGCGGATTGGTGCGCTATACGCCATTGAGGCGGAAATTCGTGGACA
ACCGCCCGATCAACGACGCGCAGTGCGGCAGCGGCGAGCCAGCCCGCTGCTAGCGCAATTTCACGCCTGGCTTAACCACACGCTGACGCAACTGCCGTCC
AAATCGGCGCTGAGCGGTGCGATCTACTATGCCCTCGCACGTTGGCAGGCGCTGACGCGCTACTGCACGGATGGCCGCATTGAGATCGACAACAACGCCG
CCGAACGCGCCCTGCGTACAGTCGCGCTGGGTCGCAAGAATTATCTGTTCGTCGGTTCCGACGCCGGCGGAGAAAGAGCGGCGGCGATCTATAGCCTGGT
CGGTTCAGCCAAGCTCAACGCATTGAACCCACAGGCTTATTTGACGCACGTGCTGGAGCGCATCGCCGACTATCCAATCAATCAGCTCGACGATTTGCTG
CCCTGGAATATTGCTCTGCCTACCTTAGAACATGAGGCCGCTTGACCCATGCCCAACGTCACCCGTTTGCGCCAGCCGGCCAGCGACCGTCTCCTGCAAC
TGCACATCGAGCTGACGTGGATAAAACCCGCCATCTGGCGCCGGGTCGCCGTGCCCGAGCGCATCACCCTGAGCAAGCTGCACCAGGTCATTCAGGTGGT
GATGGGCTGGAGCGACACCCATCTTCACGAGTTTGAAATCGCTGGCGAAAGCTACGGTATTCCCGATCCCGACTGGGGCCCTTCGGTCGTTTCGGAACAG
CGCAAGACACTGACAAAGGTGCTGTATGGGTCGAAGACGTTTCGTTACGTCTATGACTTTGGCGACAACTGGGAGCACCGGATCAAGACCGAGCGGCTGT
TGCCGGCCATTGCGTGCCCGCAAGTGCCGTATTGCATCGACGGCGCCAATGCCAGTCCGCCGGAGGATGTTGGCGGCGCACCGGGCTACGCTGATTTTCT
GGATGCGCTTGCCGACCCCGAGCATCCCGAATACTTGAACATGCTCGACTGGTACGGCGATACCTTCGATCCCACGGCTTTTGACCGCGACGCCATCAAC
CAGCGCCTGAAGCGGATTAAGACCCGAGGGTCAAGACGGTAGCGAGCGTACGCTTAC

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
390 bp	129 aa	71	460	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MGTIVDTLPSTPRRSRRPNFSVEFKRRVVEATLLPGASVALIAREHEINANLVFKWRRHYREGQLGSVAQQATLLPVNLSKAPTSSAEEAVRLPLSPGGL
VVECGRVTLRIEGVPDPQTLQLVLQQVLR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
345 bp	114 aa	457	801	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIAPPIGTRIWIAAGVTDMRRGFDGLAALVQTQLEADPFSGQIFVFRGRRGDRIKLLWWDGDGLCLFCKRLEQGRFVWPQAASGSVSLTTAQLAMLLEGI
DWRRPIRTAPVRTV

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1509 bp	502 aa	837	2345	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTCATDCLPDDIQALKALVTAQRGEIEHLKLMIAKLRRTQFGRRSEQLDGMLDQLQLTLEELQVSEAALASPPPAESHPRPPVRRKPLPAHLPREIHVHR
PDTQCPGCGGELRHLGDDVAEVLEYVPARFKVVRHVRPKLACRCCDGIVQVPAPSRPIARGLAGPGLLAHVLVSKYVDHLPLYRQSEIYAREGVSLERST
MADWVGEASRLLQPLVGRLRQHVMTSNKVHADDTPIAVLAPGQGKTKTGRLWTYVRDERPTGSSTPAAVWFAYSPDRKGEHPRAHLKGFAGTLQADGYAG
FAQLYAAGTIHEAACWAHARRKFFDLHKALASPIAAEALQRIGALYAIEAEIRGQPPDQRRAVRQRRASPLLAQFHAWLNHTLTQLPSKSALSGAIYYAL
ARWQALTRYCTDGRIEIDNNAAERALRTVALGRKNYLFVGSDAGGERAAAIYSLVGSAKLNALNPQAYLTHVLERIADYPINQLDDLLPWNIALPTLEHE
AA

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
594 bp	197 aa	2349	2942	+	No

ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MPNVTRLRQPASDRLLQLHIELTWIKPAIWRRVAVPERITLSKLHQVIQVVMGWSDTHLHEFEIAGESYGIPDPDWGPSVVSEQRKTLTKVLYGSKTFRY
VYDFGDNWEHRIKTERLLPAIACPQVPYCIDGANASPPEDVGGAPGYADFLDALADPEHPEYLNMLDWYGDTFDPTAFDRDAINQRLKRIKTRGSRR

Blast result :

Comments

ISPre3 is 56%(ORF1) aa similar to ISBcen14, 79% (ORF2) to IS883, 71% (ORF3) to ISBcen14. The ORF4 is a passenger gene annotated as hypothetical protein.

References

1] Urata,M., Miyakoshi,M., Kai,S., Maeda,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H. (2004) J. Bacteriol. 186 (20), 6815-6823
2] Maeda,K., Nojiri,H., Shintani,M., Yoshida,T., Habe,H. and Omori,T.
(2003) J. Mol. Biol. 326 (1), 21-33