ISPpy1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AM992204 | Y | Psychrobacter maritimus | Psychrobacter maritimus MR29-12. |
DNA section
IS Length : 1275 bp
Ends
IR Length : 34/50
IRL : TATAATACTCCCGGAAAACTAGACCAAAAAATTGTAGCAAATAAGATAGA
IRR : TAAGGTACTCCCCAATGTCTAGACCAAATTATCATAAAATTAAGATAGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GATAGCAAAG | AGGTGTGGTT | 0 | |
| ACAATTAAAG | AGGTGTGGTT | 0 | |
| TTTGCGCAAC | GTT | GTTGCCATTGCT | 3 |
| AGTTTGCGCA | ACGT | TGTTGCCATT | 4 |
| TAGTTATCTA | CACGA | CGGGGAGTCA | 5 |
DNA sequence
TATAATACTCCCGGAAAACTAGACCAAAAAATTGTAGCAAATAAGATAGAATAACCTTTAGAAAAAGAGGTCTATCTAATGACAAAAGTACGTAAGCGTC
ACAATGCTGAATTTAAAAGCAAAGTCGCCGTTGAAGCCATCAAAGAACACAAGACCCTCAACGAGTTAACCACAGAATATGGGGTTCATGCAACCCAAAT
CAGCAACTGGAAAAAGCAGGCTTTGGCAGTTATCCCTACTGCATTCAATACCAAACAGCAAGCCAACGAACAAGCCCAGCAAGCTATTATCGATGAACTA
CATAGGCAGCTAGGGCAAGTTATAAGCGAAAGAGACTGGCTTAAAAAAAAGTCCTTACAGCTACCCTGAGCACTCGTAAACAACTGCTAGAACCTGACAA
CAAGGATTTTAGTGTTCGTAAGCAATGTGAATTACTCAGTATCAACCGCTCAAGTCTGTACTATCAGCCAAAGCCCATCAGCGAGCTTGATATTACCCTG
ATGAACCTGCTTGACCAGCAGTACACCAAGACCCCATTTTATGGGGTTAAACGCATGACAGCGTATTTGAGGCAACTAGGCTATCAAGTGGGAGAAAAGC
GAGTTAGGCGCTTACTACGGCAAATGGGGCTAGACGCTATTTATCAGCATCCTAACACGAGTAAGCCTAACTCTGAGCATCAAGTTTACCCGTACTTGCT
TAGGCATGTACCGATTAGCCGTTGTAATCAAGTGTGGAGTACTGATATCACCTATATTCGCCTAGCCAAGGGCTTCGTATATTTGATGGCGGTGATAGAT
TGGTACAGTCGTTATGTTCTAGACTGGTCGCTATCTACCACGCTTGAGGCGGATTTCTGCGTGGATACGGTGAGCAGCTTATTGCACAATGGGTTACGCT
GTGAGATTTTTAATACAGATCAAGGCTCTCAGTTTACCAGCCCAAGATTTACCAGACCGCTCATTGAGCAGGGTATTGCTATCAGTATGGATGGTCGCGG
TAGGGCACTGGATAATATCTTTGTGGAAAGGCTTTGGCGGTCAGTGAAGTATGAATGCGTGTATTTGCGACAGTTTGAGACAGTCAGTCAGGCAAGAGCA
GGTTTGAAAGAGTATTTCGAGTTTTACAATCATGAGCGTTTACATCAGTCGCTCGATTACCATACTCCTGCACAGGTTTATCTGGCTAACAATAGTTCGG
ATAATAAATCGTTTTATCAACCCAACTCTATCTTAATTTTATGATAATTTGGTCTAGACATTGGGGAGTACCTTA
ACAATGCTGAATTTAAAAGCAAAGTCGCCGTTGAAGCCATCAAAGAACACAAGACCCTCAACGAGTTAACCACAGAATATGGGGTTCATGCAACCCAAAT
CAGCAACTGGAAAAAGCAGGCTTTGGCAGTTATCCCTACTGCATTCAATACCAAACAGCAAGCCAACGAACAAGCCCAGCAAGCTATTATCGATGAACTA
CATAGGCAGCTAGGGCAAGTTATAAGCGAAAGAGACTGGCTTAAAAAAAAGTCCTTACAGCTACCCTGAGCACTCGTAAACAACTGCTAGAACCTGACAA
CAAGGATTTTAGTGTTCGTAAGCAATGTGAATTACTCAGTATCAACCGCTCAAGTCTGTACTATCAGCCAAAGCCCATCAGCGAGCTTGATATTACCCTG
ATGAACCTGCTTGACCAGCAGTACACCAAGACCCCATTTTATGGGGTTAAACGCATGACAGCGTATTTGAGGCAACTAGGCTATCAAGTGGGAGAAAAGC
GAGTTAGGCGCTTACTACGGCAAATGGGGCTAGACGCTATTTATCAGCATCCTAACACGAGTAAGCCTAACTCTGAGCATCAAGTTTACCCGTACTTGCT
TAGGCATGTACCGATTAGCCGTTGTAATCAAGTGTGGAGTACTGATATCACCTATATTCGCCTAGCCAAGGGCTTCGTATATTTGATGGCGGTGATAGAT
TGGTACAGTCGTTATGTTCTAGACTGGTCGCTATCTACCACGCTTGAGGCGGATTTCTGCGTGGATACGGTGAGCAGCTTATTGCACAATGGGTTACGCT
GTGAGATTTTTAATACAGATCAAGGCTCTCAGTTTACCAGCCCAAGATTTACCAGACCGCTCATTGAGCAGGGTATTGCTATCAGTATGGATGGTCGCGG
TAGGGCACTGGATAATATCTTTGTGGAAAGGCTTTGGCGGTCAGTGAAGTATGAATGCGTGTATTTGCGACAGTTTGAGACAGTCAGTCAGGCAAGAGCA
GGTTTGAAAGAGTATTTCGAGTTTTACAATCATGAGCGTTTACATCAGTCGCTCGATTACCATACTCCTGCACAGGTTTATCTGGCTAACAATAGTTCGG
ATAATAAATCGTTTTATCAACCCAACTCTATCTTAATTTTATGATAATTTGGTCTAGACATTGGGGAGTACCTTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 79 | 369 | + | No |
Description : First part of the transposase
ORF sequence :
MTKVRKRHNAEFKSKVAVEAIKEHKTLNELTTEYGVHATQISNWKKQALAVIPTAFNTKQQANEQAQQAIIDELHRQLGQVISERDWLKKKSLQLP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 900 bp | 299 aa | 345 | 1244 | + | No |
Description : Second part of the transposase
ORF sequence :
KKVLTATLSTRKQLLEPDNKDFSVRKQCELLSINRSSLYYQPKPISELDITLMNLLDQQYTKTPFYGVKRMTAYLRQLGYQVGEKRVRRLLRQMGLDAIY
QHPNTSKPNSEHQVYPYLLRHVPISRCNQVWSTDITYIRLAKGFVYLMAVIDWYSRYVLDWSLSTTLEADFCVDTVSSLLHNGLRCEIFNTDQGSQFTSP
RFTRPLIEQGIAISMDGRGRALDNIFVERLWRSVKYECVYLRQFETVSQARAGLKEYFEFYNHERLHQSLDYHTPAQVYLANNSSDNKSFYQPNSILIL
QHPNTSKPNSEHQVYPYLLRHVPISRCNQVWSTDITYIRLAKGFVYLMAVIDWYSRYVLDWSLSTTLEADFCVDTVSSLLHNGLRCEIFNTDQGSQFTSP
RFTRPLIEQGIAISMDGRGRALDNIFVERLWRSVKYECVYLRQFETVSQARAGLKEYFEFYNHERLHQSLDYHTPAQVYLANNSSDNKSFYQPNSILIL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1166 bp | 388 aa | 79 | 1244 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKVRKRHNAEFKSKVAVEAIKEHKTLNELTTEYGVHATQISNWKKQALAVIPTAFNTKQQANEQAQQAIIDELHRQLGQVISERDWLKKKVLTATLSTR
KQLLEPDNKDFSVRKQCELLSINRSSLYYQPKPISELDITLMNLLDQQYTKTPFYGVKRMTAYLRQLGYQVGEKRVRRLLRQMGLDAIYQHPNTSKPNSE
HQVYPYLLRHVPISRCNQVWSTDITYIRLAKGFVYLMAVIDWYSRYVLDWSLSTTLEADFCVDTVSSLLHNGLRCEIFNTDQGSQFTSPRFTRPLIEQGI
AISMDGRGRALDNIFVERLWRSVKYECVYLRQFETVSQARAGLKEYFEFYNHERLHQSLDYHTPAQVYLANNSSDNKSFYQPNSILIL
KQLLEPDNKDFSVRKQCELLSINRSSLYYQPKPISELDITLMNLLDQQYTKTPFYGVKRMTAYLRQLGYQVGEKRVRRLLRQMGLDAIYQHPNTSKPNSE
HQVYPYLLRHVPISRCNQVWSTDITYIRLAKGFVYLMAVIDWYSRYVLDWSLSTTLEADFCVDTVSSLLHNGLRCEIFNTDQGSQFTSPRFTRPLIEQGI
AISMDGRGRALDNIFVERLWRSVKYECVYLRQFETVSQARAGLKEYFEFYNHERLHQSLDYHTPAQVYLANNSSDNKSFYQPNSILIL
Blast result :
Comments
ISPpy1 is 72% aa similar to ISAzs33.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
This IS has atypic IRs: it begins with 'TA' instead of 'TG'.
The two copies of ISPpy1 flank the antibiotic resistance region as transposon Tn5080.
Tn5080 codes for tetracycline and streptomycin resistance.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
This IS has atypic IRs: it begins with 'TA' instead of 'TG'.
The two copies of ISPpy1 flank the antibiotic resistance region as transposon Tn5080.
Tn5080 codes for tetracycline and streptomycin resistance.
References
1] Petrova, M., Gorlenko, Zh., and Mindlin, S. (2009) FEMS Microbiol Lett. 296, 190-197
2] Petrova, M., Gorlenko, Zh., Shcherbatova, N., and Mindlin, S. (2012) Genetika 48, 324-332. Russian.
2] Petrova, M., Gorlenko, Zh., Shcherbatova, N., and Mindlin, S. (2012) Genetika 48, 324-332. Russian.