ISPpu10

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Pseudomonas putida
Pseudomonas putida KT2440
DNA section
IS Length : 1314 bp

Ends


Left end : TGTATGGTAGGACTTGAAGGGAGGAGGAGGGCCATAACTCGCTTCTGACTGTTCGCGCAGTACAGACCGTAGGAGAAATCACCCCTCCTCCTTTCAACAC
Right end : AATGCTGGGGAGAAATTCTCTGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCCTCCGGCCATAGAAT

Insertion site


Left flankDirect repeatRight flankDR Length
TACCGTATTCGCGGGTAAACCCGCTCCTACAGTGG2
CGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGCT2
TGCCTGCTTCGCGGGTAAACCCGCTCCTACGGGGG2
CGGCTCTTTCGCGGGTAAACCCGCTCCTACAGGGG2
TGGCCTCTTCGCGGGTAAACCCGCTCCTACATCGG2
CACCGTATTCGCGGGTAAACCCGCTCCTACCCCAA2
TGGCCCTTTCGCGGGTAAACCCGCTCCTACATGGG2

DNA sequence

TGTATGGTAGGACTTGAAGGGAGGAGGAGGGCCATAACTCGCTTCTGACTGTTCGCGCAGTACAGACCGTAGGAGAAATCACCCCTCCTCCTTTCAACAC
CCAAGCGCCGATAAGGAATGCCTCGCGAGCAAGCGACAATAGAAGCAAGCCTGCGCCAGGGTGTGCCCTTCAAGTCACACTACGCTACAAGGAGCAGCTA
TTCATGGCAATGTCCGCAATCCCAATCGAAGCTGGCGTGGACACCTCCAAAGATGAACTGGTGATTCAAGCCGCTCCGAATACCAAATCCTTCGCCATCC
CTAACACTCCGAAAGCAATCAAAGCCTGGCTCAAGACGCTGCCCAAAGGCTCAGCGCTGGCCATCGAAGCGACCAGTACCTATCACATGGAAATGGCGGA
GCAAGCCCATGCTGCGGGCTTCGCGGTATACGTTATCGATGGGCTGAGGCTGAGTAAGTACCGGGAAAGTGTTGCTATACGGGCAAAAACAGATGCCCAT
GATGCCGCTTTGCTCGCCCGCTTCCTGAGCAACGAGCGGGGCTCTTTGAAGGCTTGGACTCCGCCACCAGCCGGGCATCGTGAGATCCAGGTGCTGCTGC
GCCGCCGCGCCAAACTTGTAGCAGTGCGCGGCATGCTGCGGATGAGCTTGTCGGGCGACAAATTGTTTGCCTCAGAACTCAAACGTGTTGAGGAAGTGCT
CGAACGCATAGAGCTTTCGCTGGAAAAACAGCTGCGTGCGGTGATCAAGAAGGCGGGGCTTTCCGATCAGATGCGCCGTGTTCAGAGGCTTCCGGGTGTT
GGTTTCTTAACGGCTGCCGGCCTGGTGATGTCCTTCATGCGCGGCGAGTTCAAGAACAGCGACGCCTTTGTCGCTTATCTAGGCATGGATGTAACGGTTT
CCCAATCCGGTAAATGGGCAGGCAGAGGGAAGTTGAGCAAGCGCGGAGACTCGGAGGTCCGTAGACTCCTGTACAACGCCTCGATGAGCGGCAGTCGAAC
CGCGACCTGGAAGCAGTATTACGCCCATCACCAAGCCCGGGGGAAGAAAACCACTCAAGCCTTGGTGATCCTTGCACGTCGCTTGGCTCGGCTGGCCTTC
GGCCTGATGAGGCATCAGGCCGACTGGAAGCCCGAGATATATACCGGAGGTGCCAAGCCGGCCAGCTGAGGCTGGGGCTGCAACGCAGCCCGCCCAAACT
GACGGGCAGTATCCAATGCTGGGGAGAAATTCTCTGGCACGCTGACTCGTACCTCGAGGTTGCTGAGAGAGGCCGATGGCGTTTTTACTGAAAATTTGCC
TCCGGCCATAGAAT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
966 bp321 aa2041169+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MAMSAIPIEAGVDTSKDELVIQAAPNTKSFAIPNTPKAIKAWLKTLPKGSALAIEATSTYHMEMAEQAHAAGFAVYVIDGLRLSKYRESVAIRAKTDAHD
AALLARFLSNERGSLKAWTPPPAGHREIQVLLRRRAKLVAVRGMLRMSLSGDKLFASELKRVEEVLERIELSLEKQLRAVIKKAGLSDQMRRVQRLPGVG
FLTAAGLVMSFMRGEFKNSDAFVAYLGMDVTVSQSGKWAGRGKLSKRGDSEVRRLLYNASMSGSRTATWKQYYAHHQARGKKTTQALVILARRLARLAFG
LMRHQADWKPEIYTGGAKPAS

 

Blast result :
Comments
There are 7 copies of ISPpu10 in this strain. he conserved target site is a species-specific repetitive extragenic palindromic (REP) sequence identified previously in Pseudomonas putida KT2440 (Aranda-Olmedo et.al. Nucleic Acids Res. 2002 Apr 15;30(8):1826-33.).
ISPpu10 is 45% aa similar to IS1000A.
References
1] DeBoy., R. (2002) Direct submission