ISPpr14
- Family IS66
- Group ISBst12
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006371 | ND | Photobacterium profundum | Photobacterium profundum SS9 |
DNA section
IS Length : 1568 bp
Ends
IR Length : 19/27
IRL : GTAACCGTTCACCAGCCCCCTATTGACACAAAAAAATGTGATGGAGCGCA
IRR : GTAAGCGTTCACCAGAGTAGCTTGACTGCTACCCTGAACAAAGATTGAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAACAAAACC | CGTATTTCT | TCGATAAGTT | 9 |
| GATCAGAGAA | CTGGAACG | AGCGTAATAC | 8 |
| AACAGGTGCT | GAATTTTC | TGGTATACCA | 8 |
| GCCTTGGTAC | ACTTTATC | TTTATTAGCG | 8 |
| GCAGCAATGC | GAACATCG | AAGCTTGAGA | 8 |
| AACGCAGCTG | GCGAAAGG | CGCATTTCGC | 8 |
| TGAGTATGCC | GTTTGATT | AGCTTCTGAT | 8 |
| CACGTAGAGC | CTTTTGTC | TCAATGAAAA | 8 |
| GCTGAAACGA | GCATCTTG | AGGTAACGTA | 8 |
DNA sequence
GTAACCGTTCACCAGCCCCCTATTGACACAAAAAAATGTGATGGAGCGCACTTGATCCACATTTGGCTATTGAGTGATCCTCCCCACACGTCATGCTTAC
CAGTATGAAAAAGAAATTATCATTTAAAGAAGAACCACCGACGGTAAATAGCCTCAATGAAGCTCAAGCTCTTATCCAGGAGCTATGGGAGAAATTGCGG
CACTACGAAGACAAGCTCTCGACGAGTTCTAAAAACTCATCAAAGTCGCCATCATCTGATGGCCCAAAAGAGAGGGCTGAGCGAAAAAAGCTGAAAGCCC
TCGTAGCGGCAATAAGATTGGAGCTCAACAAGGTCACGAGGGCAAACGGCGAAAACTGAGCAAACTCAAAAAGACAGATACTGTTGTACCCTGTGTGCCC
AGTTCACTGTGCCCTTGTTGTGGCGATAGTCATATCGCGATTAATCAAAAACCATCCTATCGGCATCAAGTGCATGAAATCCCTGAGCCAATTGTAAATA
TTACTGAATATCAAGTCTTTCATGGCCGGTGTCAAAATTGCCAGACTTCCGTTAAGGGGAAGCGGCCTCAAGATACCCCACAGGGAATAATGGGCCCTAA
CCTCATGAGCTACATTGCCCTGCTGGCTGGACAGTTTCATCTCAGCGTTCGTAAAATACAAGAATTACTCAAGCTGCAGTTAGGCACCTCCTTTTCCACG
GGCGCTATCTCCGAGGCTCAAGGAAAAGTATCATCGATGCTTACGCCTTTACATCAAGCCGTTCGAGACGCCATTCAAAAAGCGCCAATGGTTCATATTG
ATGAGACTTCTCACATCAGAAATGGTGAAAGCAGCCTACGCTGGTGTTGGCTGATGTCGAGTGATGATTGGGTCTATGAACGTGTTTTGTTTTCACGCTC
AACCCACTCGGCAAAAGTGATGCTGAATGAAAAATTTGCTGGGGTTGTTATCTCCGATCAATGTGCCAGCTATAATTGGCTCAATCCTGAAAAACATCAA
TTTTGCTTAGGCCACCTAAAGCGAAATCTCCAACAAATGGCCGATTACAGTGGGGGTGGGTTAACCGCTTTTATTGGCAAACGACTGACTCTGCTTATCA
ATATGATTTTTCGCACTCAACATTGTTATGAAGAAGAAAACATTTCAGCGGAGATATACTTTCGGAGAATGAAGCGATTGAGAAAGAGCTTAACGCGCTG
GTTACACAAAGGCGCGGATGTCACAACATCTAGATATAGCGGTCGATGTAAATTCATTCTTAAGCACGAAGTCAGTTTGTGGGTATTTCTGACGGCTCCC
TTAACAATCCCATTAACCAACAATGAAGCCGAGCGCCGGTTACGTGGAAGCGTGATCATGCGAAAAATCAGTTTTGGTACAAGCTCCGATCGCGGTGATA
AGTTTCGAGGCCGAATACACACTCTTGTTGAAACCTGCAAGAAACGAAGTATGTCACCATTTATCGCTCTTCAACAGATTGTCAATGCTGTCGTGAAAAA
ACAGCCATATCCTGACATCTTCAATCTTTGTTCAGGGTAGCAGTCAAGCTACTCTGGTGAACGCTTAC
CAGTATGAAAAAGAAATTATCATTTAAAGAAGAACCACCGACGGTAAATAGCCTCAATGAAGCTCAAGCTCTTATCCAGGAGCTATGGGAGAAATTGCGG
CACTACGAAGACAAGCTCTCGACGAGTTCTAAAAACTCATCAAAGTCGCCATCATCTGATGGCCCAAAAGAGAGGGCTGAGCGAAAAAAGCTGAAAGCCC
TCGTAGCGGCAATAAGATTGGAGCTCAACAAGGTCACGAGGGCAAACGGCGAAAACTGAGCAAACTCAAAAAGACAGATACTGTTGTACCCTGTGTGCCC
AGTTCACTGTGCCCTTGTTGTGGCGATAGTCATATCGCGATTAATCAAAAACCATCCTATCGGCATCAAGTGCATGAAATCCCTGAGCCAATTGTAAATA
TTACTGAATATCAAGTCTTTCATGGCCGGTGTCAAAATTGCCAGACTTCCGTTAAGGGGAAGCGGCCTCAAGATACCCCACAGGGAATAATGGGCCCTAA
CCTCATGAGCTACATTGCCCTGCTGGCTGGACAGTTTCATCTCAGCGTTCGTAAAATACAAGAATTACTCAAGCTGCAGTTAGGCACCTCCTTTTCCACG
GGCGCTATCTCCGAGGCTCAAGGAAAAGTATCATCGATGCTTACGCCTTTACATCAAGCCGTTCGAGACGCCATTCAAAAAGCGCCAATGGTTCATATTG
ATGAGACTTCTCACATCAGAAATGGTGAAAGCAGCCTACGCTGGTGTTGGCTGATGTCGAGTGATGATTGGGTCTATGAACGTGTTTTGTTTTCACGCTC
AACCCACTCGGCAAAAGTGATGCTGAATGAAAAATTTGCTGGGGTTGTTATCTCCGATCAATGTGCCAGCTATAATTGGCTCAATCCTGAAAAACATCAA
TTTTGCTTAGGCCACCTAAAGCGAAATCTCCAACAAATGGCCGATTACAGTGGGGGTGGGTTAACCGCTTTTATTGGCAAACGACTGACTCTGCTTATCA
ATATGATTTTTCGCACTCAACATTGTTATGAAGAAGAAAACATTTCAGCGGAGATATACTTTCGGAGAATGAAGCGATTGAGAAAGAGCTTAACGCGCTG
GTTACACAAAGGCGCGGATGTCACAACATCTAGATATAGCGGTCGATGTAAATTCATTCTTAAGCACGAAGTCAGTTTGTGGGTATTTCTGACGGCTCCC
TTAACAATCCCATTAACCAACAATGAAGCCGAGCGCCGGTTACGTGGAAGCGTGATCATGCGAAAAATCAGTTTTGGTACAAGCTCCGATCGCGGTGATA
AGTTTCGAGGCCGAATACACACTCTTGTTGAAACCTGCAAGAAACGAAGTATGTCACCATTTATCGCTCTTCAACAGATTGTCAATGCTGTCGTGAAAAA
ACAGCCATATCCTGACATCTTCAATCTTTGTTCAGGGTAGCAGTCAAGCTACTCTGGTGAACGCTTAC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 255 bp | 84 aa | 105 | 359 | + | No |
Chemistry : DDE
ORF sequence :
MKKKLSFKEEPPTVNSLNEAQALIQELWEKLRHYEDKLSTSSKNSSKSPSSDGPKERAERKKLKALVAAIRLELNKVTRANGEN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1260 bp | 419 aa | 281 | 1540 | + | No |
Chemistry : DDE
ORF sequence :
AKKAESPRSGNKIGAQQGHEGKRRKLSKLKKTDTVVPCVPSSLCPCCGDSHIAINQKPSYRHQVHEIPEPIVNITEYQVFHGRCQNCQTSVKGKRPQDTP
QGIMGPNLMSYIALLAGQFHLSVRKIQELLKLQLGTSFSTGAISEAQGKVSSMLTPLHQAVRDAIQKAPMVHIDETSHIRNGESSLRWCWLMSSDDWVYE
RVLFSRSTHSAKVMLNEKFAGVVISDQCASYNWLNPEKHQFCLGHLKRNLQQMADYSGGGLTAFIGKRLTLLINMIFRTQHCYEEENISAEIYFRRMKRL
RKSLTRWLHKGADVTTSRYSGRCKFILKHEVSLWVFLTAPLTIPLTNNEAERRLRGSVIMRKISFGTSSDRGDKFRGRIHTLVETCKKRSMSPFIALQQI
VNAVVKKQPYPDIFNLCSG
QGIMGPNLMSYIALLAGQFHLSVRKIQELLKLQLGTSFSTGAISEAQGKVSSMLTPLHQAVRDAIQKAPMVHIDETSHIRNGESSLRWCWLMSSDDWVYE
RVLFSRSTHSAKVMLNEKFAGVVISDQCASYNWLNPEKHQFCLGHLKRNLQQMADYSGGGLTAFIGKRLTLLINMIFRTQHCYEEENISAEIYFRRMKRL
RKSLTRWLHKGADVTTSRYSGRCKFILKHEVSLWVFLTAPLTIPLTNNEAERRLRGSVIMRKISFGTSSDRGDKFRGRIHTLVETCKKRSMSPFIALQQI
VNAVVKKQPYPDIFNLCSG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1436 bp | 478 aa | 105 | 1540 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKKLSFKEEPPTVNSLNEAQALIQELWEKLRHYEDKLSTSSKNSSKSPSSDGPKERAERKKAESPRSGNKIGAQQGHEGKRRKLSKLKKTDTVVPCVPS
SLCPCCGDSHIAINQKPSYRHQVHEIPEPIVNITEYQVFHGRCQNCQTSVKGKRPQDTPQGIMGPNLMSYIALLAGQFHLSVRKIQELLKLQLGTSFSTG
AISEAQGKVSSMLTPLHQAVRDAIQKAPMVHIDETSHIRNGESSLRWCWLMSSDDWVYERVLFSRSTHSAKVMLNEKFAGVVISDQCASYNWLNPEKHQF
CLGHLKRNLQQMADYSGGGLTAFIGKRLTLLINMIFRTQHCYEEENISAEIYFRRMKRLRKSLTRWLHKGADVTTSRYSGRCKFILKHEVSLWVFLTAPL
TIPLTNNEAERRLRGSVIMRKISFGTSSDRGDKFRGRIHTLVETCKKRSMSPFIALQQIVNAVVKKQPYPDIFNLCSG
SLCPCCGDSHIAINQKPSYRHQVHEIPEPIVNITEYQVFHGRCQNCQTSVKGKRPQDTPQGIMGPNLMSYIALLAGQFHLSVRKIQELLKLQLGTSFSTG
AISEAQGKVSSMLTPLHQAVRDAIQKAPMVHIDETSHIRNGESSLRWCWLMSSDDWVYERVLFSRSTHSAKVMLNEKFAGVVISDQCASYNWLNPEKHQF
CLGHLKRNLQQMADYSGGGLTAFIGKRLTLLINMIFRTQHCYEEENISAEIYFRRMKRLRKSLTRWLHKGADVTTSRYSGRCKFILKHEVSLWVFLTAPL
TIPLTNNEAERRLRGSVIMRKISFGTSSDRGDKFRGRIHTLVETCKKRSMSPFIALQQIVNAVVKKQPYPDIFNLCSG
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPpr14 (orfAB) is 75% aa similar to ISSbe3.
ISPpr14 (orfAB) is 75% aa similar to ISSbe3.
References
1] ISfinder annotation (2008).
2] Vezzi,A., Campanaro,S., D'Angelo,M., Simonato,F., Vitulo,N., Lauro,F.M., Cestaro,A., Malacrida,G., Simionati,B., Cannata,N., Romualdi,C., Bartlett,D.H. and Valle,G. (2005) Science 307 (5714), 1459-1461
3] Cestaro,A. (2004) Direct Submission GenBank.
2] Vezzi,A., Campanaro,S., D'Angelo,M., Simonato,F., Vitulo,N., Lauro,F.M., Cestaro,A., Malacrida,G., Simionati,B., Cannata,N., Romualdi,C., Bartlett,D.H. and Valle,G. (2005) Science 307 (5714), 1459-1461
3] Cestaro,A. (2004) Direct Submission GenBank.