ISfinder Mirror Site

ISPfr21

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Propionibacterium freudenreichii	Propionibacterium freudenreichii

DNA section

IS Length : 2632 bp

Ends

IR Length : 21/22

IRL : TGTACTGACCGGACACGTTGGTTGAGGAGCGAAACGCGGTTCGGTCCTGT
IRR : TGTACTGACCGGAGACGTTGGTCAATCGTGAGAAGGGTGGCTGGTTGCCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GACCGGCGCA	GCTAGG	CCCACAACAA	6

DNA sequence

TGTACTGACCGGACACGTTGGTTGAGGAGCGAAACGCGGTTCGGTCCTGTTGTGAGGGAGAACTCCTCACCGTCGGACGAGGAGTTCCTGTGATGCGTTG
CAGGCTTGGGCGAACACCCTTGGGTCGCCTTGGATTGACGCGGTGACCTGCGCTCCTTCATGGAGCAGGTGCAGCAGGGTCCCAGTGTTCTCGTCGCCGC
TGGCGATCTGCGTGAACAGGGCCAGCATCCAGGCTTTCTCTGCCCGGATGAACTTGGTGGCCTCATGGTCGCTGGCGCCGATCTCGGACCACGCGTTGAC
GAACGCGCAACCGCGGGGGGTGCTGGTCGCCCATTGGGCAGTGTGCTGGTAGACGATGTCCAAGGCTTGAGCTGGGGAGGCATCGGTCAATGTTTCCAGG
AGTTCGCCTTGCCAGGCGTGGGCGCGTTGTTGTAGGTAGCAGACGATCAACCGATCCTTGGATCCGTAGCGTTGGTACAAGGTCCTCTTTGTGGTCCCTG
CGTGCTCGACGATGCTGTCGACACCGGTGGCCGTGATCCCGTGGTCGCGGAAGAGTTCGGCGGCAGCATCGAGAATCCGGCGGCCGACCGGCGTGGTGTC
GACAGGCTCGAATACCTCGAGGTCGATGTCCATGCTTTTGTCCCCTCAGCCGACGGTGGTGCACTGACAGTATACCGACCGGTGTGTTTACCTGACTGGA
TGCGACGGGTCATGTGTGTAGCGTTTGTGGTGGCGTGGAGTTCCGGGTTCGTCGGGGCGACGCTGGCGGACCGGACAGGCGTGGGGACGTGGTCCCTGCT
GGCCTGGCGTTACCTGGCGACCGCCCTCGTACTCCTCGTCGGGTGTCTCCTGGTTCCTTCACTGCGCCACTCTTTCTGCGCGCTGGGTCGACGTGAGGTG
TTGCAACAGGTCATCCTGTCCGTCTTGTCTCATGTGCTCTTCCTGGGCGGTGTCTTCCTCGCCGCCGAAGAGGGGCTGGATGCCGGCTTGTCGGCGTTGG
TGTGTGCACTCCAGCCGCTGATCGTGACCGTGGCAGGACGAATCTTTTTCGGTGACCGGGTCAGAATCGTCCAGTGGGTTGGCTTGCTCATTGCCCTGGG
TGGCGTCTCCCTGAGCGTGGGAGGCATCAGCGCAGCGGGGGTGGGAAGCGTGGGCCTGGTAGCAATGAGTCTGCTGGGGCTGTCTACGGCCTCACTGCTG
GAACGCTCGTGGAATCCCCAGGTGCCAGTGACGGTCAGCCTGACCGTGCAGGTGAGCGTCGCGGCCGTGATCTTCAGCGGTGTCGCCCTCGTGACCGGCG
GTCTCGCACTGCAGGTCACGGGAGCACTGGTGCTGGCCATCAGTTGGCTGGTTCTCTTGTCAGGTCTCGGCGGCTACGCCACGTTCATCTGGTGCCTACG
CCACGTGGGAGCCACCACGACCAGCACTCTGCTCTACCTGACTGCACCGGTCACGATGCTGTGGGCGTGGTGCATGTTTGGCCAGCAGCCCTCGCCCCTG
CAGTGGATTGGGCTCGCGGTCGTCCTGAGCGGCGTCGGCCTCGCGACAAGACGCACCAACCACCGTGAGCAAGGCACAGCCTGACCACCGGCCCCAATGC
GGCCGGATCGTACGCAACGACACCACGTCAATATCAGGAGCAAGGCTTCCATGACCCACACGAATGCTGCTCTCACACCGCGCCACCGGCTGATCGTCGC
TCGGCTGGTCGTCGAGGAAGACTGGCCGGTCAGCGAGGTCGCTGCCCGGTTCCAGGTGTCTTGGCCGACCGTGAAGCGCTGGGCCGACCGCTACCGGGCC
GGCCAGTCCATGTAGGACCGCTCCTCGCGGCCGCAGCACTCGCCGAACAAGACCGACCAGGCCACCACGCGGTGCTGTATCCAGCTCCGGCTACGTCTGC
GCGAGGGGCCGGTTCAGCTCGCGTGCCGGCTCGGCGTCGCGCCGTCCACGGTCCACCGGATCTTGGTCGACGCCCGCCTGAACCGGCTGTCGAACGTTGA
CCGCGCGACTGGGGAACCCGTCCGTCGCTACGAACACGATCACCCCGGGGCCATGCTGCACGTCGATGTGAAGAAGCTCGGCAACATCCCCGACGGCGGG
GGCTGGCGTTACGTCGGCCGCCGGCAAGGCGAGAAGAACCGGGCCGCCACGCCTGACAAGGCCAAGAACAAGTGGCGGGACCCTTTGATGGGCAAGGCCT
ATGTCCACACCGCCATCGACGACCACTCCCGTGTCGCCTATGCCGAAGTGCACGACGACGAAACCGCCTTCACCGCCGCCGCTGTTCTGGTGAGGGCTGT
CGAGTGGTTCAACGCCTGCGGGGTCACGGTGGAGCGGGTGCTGTCTGACAACGGTGGCGCTTACCGGTCGCACCTGTGGCGAGACACCTGCGCCGAGCTG
GGCATCCGGCACAAGCGCACCAGGCCGTATCGACCGCAGACCAACGGCAAGATCGAGCGCTTCCGCCGAACCCTGTCCGACGGCTGGGCCTATGCCCGCT
GCTACACCTCTGAGACGGAGCGCCGCGGGGAGCTCGAGGGCTGGCTGCACAACTACAACCATCACCGACCCCACACGGCTTGCGGCAACCAGCCACCCTT
CTCACGATTGACCAACGTCTCCGGTCAGTACA

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
567 bp	188 aa	633	67	-	No

ORF function : Passenger Gene
Annotation : Transcriptional regulator, TetR familyDescription : Transcriptional Regulator factor

ORF sequence :

MDIDLEVFEPVDTTPVGRRILDAAAELFRDHGITATGVDSIVEHAGTTKRTLYQRYGSKDRLIVCYLQQRAHAWQGELLETLTDASPAQALDIVYQHTAQ
WATSTPRGCAFVNAWSEIGASDHEATKFIRAEKAWMLALFTQIASGDENTGTLLHLLHEGAQVTASIQGDPRVFAQACNASQELLVRR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
858 bp	285 aa	727	1584	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MVAWSSGFVGATLADRTGVGTWSLLAWRYLATALVLLVGCLLVPSLRHSFCALGRREVLQQVILSVLSHVLFLGGVFLAAEEGLDAGLSALVCALQPLIV
TVAGRIFFGDRVRIVQWVGLLIALGGVSLSVGGISAAGVGSVGLVAMSLLGLSTASLLERSWNPQVPVTVSLTVQVSVAAVIFSGVALVTGGLALQVTGA
LVLAISWLVLLSGLGGYATFIWCLRHVGATTTSTLLYLTAPVTMLWAWCMFGQQPSPLQWIGLAVVLSGVGLATRRTNHREQGTA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
987 bp	328 aa	1651	2637	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTHTNAALTPRHRLIVARLVVEEDWPVSEVAARFQVSWPTVKRWADRYRAGQSM*DRSSRPQHSPNKTDQATTRCCIQLRLRLREGPVQLACRLGVAPST
VHRILVDARLNRLSNVDRATGEPVRRYEHDHPGAMLHVDVKKLGNIPDGGGWRYVGRRQGEKNRAATPDKAKNKWRDPLMGKAYVHTAIDDHSRVAYAEV
HDDETAFTAAAVLVRAVEWFNACGVTVERVLSDNGGAYRSHLWRDTCAELGIRHKRTRPYRPQTNGKIERFRRTLSDGWAYARCYTSETERRGELEGWLH
NYNHHRPHTACGNQPPFSRLTNVSGQYS

Blast result :

Comments

ISPfr21 is 85%(ORFC : the transposase) aa similar to ISKrh1. ORFA and ORFB are passenger gene annotated respectively as transcriptional regulator and hypothetical protein.
The transposase had a STOP codon in frame and was probably inactive.

References

1] ISfinder annotation (2009)