ISPa25
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| DQ393782 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa |
DNA section
IS Length : 1409 bp
Ends
IR Length : 15/18
IRL : tgatggaATGGACTCCTCCCGCATCGCCCACACGGCTTCGGGAGCCAGAC
IRR : ----tatATGGACTCCTCCGTCAACCAGTAATTGCGCGATGGATCCTGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGACCTTGAG | CGACTTGTTA | 0 |
DNA sequence
TGATGGAATGGACTCCTCCCGCATCGCCCACACGGCTTCGGGAGCCAGACTGATATTTCCATCAGGACACGGGATCACGAGAGGAGTCCGCCATGCATGC
TACGACCGTTGCGGTTGATCTTGCAAAAGACGTATTCGAGCTGGCATTTGCCGATGGCCAGGGTCGGGTGATCGAACGGAAACGGCTGACGCGGCGGGCG
TTTGCCCGCGCGTTCGAGCAGCGACCGCCGCTGCGGATCGTGATGGAAGCCTGCGGCAGCGCGCACCACTGGGCGCGGCGCTTCGTGCGGCAGGGGCACG
CGGTGCGGCTGCTGCCGGCCAGCGACGTGCGCCCTTACGTGCGTGGCAACAAGACCGATCGCAACGATGCATCCGGCATCCTCGAGGCCGACCGCTGCGG
CGGCATCGCCAGCGTGCCGGTGAAGACGCCGGAGCAGCAGGCCATCCAGGCGCAGCACCGGTTGCGTGAGCACCTCAAGCGCGAGCGCACGGCGACGATC
AATCTGCTGCGCGGCGTGCTGCGCGAGTTCGGCATCGTCATCGCACAGGGCGCGGCCCGGGTGTCGGCGCAAGTGCGCGATGCGCTGGAGGACGGCGACA
ACGATCTGCCGATGCCGTTGCGGCACAGCCTGGGCGAGCAGCTGCAGCGGCTGCTGCACATCCAGGCAGACATGCGGGCCATCGAACAACGGCTCGACGA
GTACGCGCAGCGCGATGTCGCCGTGCAGCGCTACCAGCGCGTGCCGGGAGTGGGCCTGCTCACCGCCACGGCCCTGCGCGCCGGTGCCGGCGACCTGGCG
CGGTTCAGGAGCGGTCGCCAGTTGTCGGCGTGGCTGGGCCTGACCCCGCGCGAGCATTCCTCTGGCCAGCAGCGACGGCTGGGGCGGATGACCAAGCGAG
GCGATGCCTACCTGCGCACCTTGTTCATTCACGGCGCCCGCGCGGTGTTGCAAGGCGCGCGGCTCAAGCACAAACGCGGCCAGCCGCTGGATCCGCTGCA
GGCCTGGGCGCTGGGCGTGCAGGACAAGCGCGGCCACAACAAGGCGGCCTGCGCGCTGGCCAACAAGCTGGCGCGGCGGCTGTGGGCAATGGAGCACCAC
GGCAAGCCCTTCGATGCACACCACGTCAGCCGGCAACCGCTCGCCGCCTGACCCGCCACTTCACCGTTCCACCCGATCAGGCATTGCCGGAAACATCTTG
CTCATGGCGAAGGGTTCGGTCCCGAAGCGGAACACGCCGATAACAATGCCGGCCCACGAGGCCGCTTGAACGATAGGCACCCGCTTCGCGACTTCCATGA
TGGCCCGGGCGCTTGCGCCCACCGCAAGGCCGAATATACGAATGCAACCGCACCGCTTCCCGACCGCAGGATCCATCGCGCAATTACTGGTTGACGGAGG
AGTCCATATA
TACGACCGTTGCGGTTGATCTTGCAAAAGACGTATTCGAGCTGGCATTTGCCGATGGCCAGGGTCGGGTGATCGAACGGAAACGGCTGACGCGGCGGGCG
TTTGCCCGCGCGTTCGAGCAGCGACCGCCGCTGCGGATCGTGATGGAAGCCTGCGGCAGCGCGCACCACTGGGCGCGGCGCTTCGTGCGGCAGGGGCACG
CGGTGCGGCTGCTGCCGGCCAGCGACGTGCGCCCTTACGTGCGTGGCAACAAGACCGATCGCAACGATGCATCCGGCATCCTCGAGGCCGACCGCTGCGG
CGGCATCGCCAGCGTGCCGGTGAAGACGCCGGAGCAGCAGGCCATCCAGGCGCAGCACCGGTTGCGTGAGCACCTCAAGCGCGAGCGCACGGCGACGATC
AATCTGCTGCGCGGCGTGCTGCGCGAGTTCGGCATCGTCATCGCACAGGGCGCGGCCCGGGTGTCGGCGCAAGTGCGCGATGCGCTGGAGGACGGCGACA
ACGATCTGCCGATGCCGTTGCGGCACAGCCTGGGCGAGCAGCTGCAGCGGCTGCTGCACATCCAGGCAGACATGCGGGCCATCGAACAACGGCTCGACGA
GTACGCGCAGCGCGATGTCGCCGTGCAGCGCTACCAGCGCGTGCCGGGAGTGGGCCTGCTCACCGCCACGGCCCTGCGCGCCGGTGCCGGCGACCTGGCG
CGGTTCAGGAGCGGTCGCCAGTTGTCGGCGTGGCTGGGCCTGACCCCGCGCGAGCATTCCTCTGGCCAGCAGCGACGGCTGGGGCGGATGACCAAGCGAG
GCGATGCCTACCTGCGCACCTTGTTCATTCACGGCGCCCGCGCGGTGTTGCAAGGCGCGCGGCTCAAGCACAAACGCGGCCAGCCGCTGGATCCGCTGCA
GGCCTGGGCGCTGGGCGTGCAGGACAAGCGCGGCCACAACAAGGCGGCCTGCGCGCTGGCCAACAAGCTGGCGCGGCGGCTGTGGGCAATGGAGCACCAC
GGCAAGCCCTTCGATGCACACCACGTCAGCCGGCAACCGCTCGCCGCCTGACCCGCCACTTCACCGTTCCACCCGATCAGGCATTGCCGGAAACATCTTG
CTCATGGCGAAGGGTTCGGTCCCGAAGCGGAACACGCCGATAACAATGCCGGCCCACGAGGCCGCTTGAACGATAGGCACCCGCTTCGCGACTTCCATGA
TGGCCCGGGCGCTTGCGCCCACCGCAAGGCCGAATATACGAATGCAACCGCACCGCTTCCCGACCGCAGGATCCATCGCGCAATTACTGGTTGACGGAGG
AGTCCATATA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 966 bp | 322 aa | 93 | 1058 | + | No |
Chemistry : DEDD
ORF sequence :
MHATTVAVDLAKDVFELAFADGQGRVIERKRLTRRAFARAFEQRPPLRIVMEACGSAHHWARRFVRQGHAVRLLPASDVRPYVRGNKTDRNDASGILEAD
RCGGIASVPVKTPEQQAIQAQHRLREHLKRERTATINLLRGVLREFGIVIAQGAARVSAQVRDALEDGDNDLPMPLRHSLGEQLQRLLHIQADMRAIEQR
LDEYAQRDVAVQRYQRVPGVGLLTATALRAGAGDLARFRSGRQLSAWLGLTPREHSSGQQRRLGRMTKRGDAYLRTLFIHGARAVLQGARLKHKRGQPLD
PLQAWALGVQDKRGHNKAACALANKLARRLWAMEHHGKPFDAHHVSRQPLAA
RCGGIASVPVKTPEQQAIQAQHRLREHLKRERTATINLLRGVLREFGIVIAQGAARVSAQVRDALEDGDNDLPMPLRHSLGEQLQRLLHIQADMRAIEQR
LDEYAQRDVAVQRYQRVPGVGLLTATALRAGAGDLARFRSGRQLSAWLGLTPREHSSGQQRRLGRMTKRGDAYLRTLFIHGARAVLQGARLKHKRGQPLD
PLQAWALGVQDKRGHNKAACALANKLARRLWAMEHHGKPFDAHHVSRQPLAA
Blast result :
Comments
The IR of ISPa25 are not at its termini. In the IS sequence, as given, 7nt separate IRl from the left-hand end of the element and 3nt separates IRr from the right-hand end. Like other IS1111 family, ISKpn4 subgroup members, ISPa25 targets a specific site in the 59be (or attC sites) in gene cassettes associated with integrons and is inserted in only one orientation. ISPa25 is also found in GenBank Accession Number DQ902344 from K. pneumoniae.
ISPa25 is 60% aa similar to ISPst6.
ISPa25 is 60% aa similar to ISPst6.
References
1] Partridge and Hall (2003) J. Bacteriol. 185, 6371-63841
2] Post, V. and Hall, R.M. (2007) unpublished
2] Post, V. and Hall, R.M. (2007) unpublished